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1.
Urologe A ; 53(5): 715-24, 2014 May.
Artigo em Alemão | MEDLINE | ID: mdl-24700162

RESUMO

OBJECTIVE: Measurement of prostate-specific antigen (PSA) is not only used as a screening instrument by urologists, but also by general practitioners and internal specialists (GP-IS). Until now, there are neither data on the approach of German GP-IS in practicing this nor have data been classified in the context of available international literature on this topic. MATERIALS AND METHODS: Between May and December 2012, a questionnaire containing 16 items was sent to 600 GP-IS in Brandenburg and Berlin. The response rate was 65% (392/600). Six indicator questions (IQ1-6) were selected and results were set in the context of available international data. The quality of present studies was evaluated by the Harden criteria. RESULTS: Of the 392 responding physicians, 317 (81%) declared that they would use PSA testing for early detection of PCA (IQ1) and, thus, formed the study group. Of these GP-IS, 38% consider an age between 41 and 50 years as suitable for testing begin (IQ2), while 53% and 14% of the GP-IS perform early detection until the age of 80 and 90 years, respectively (IQ3). A rigid PSA cut-off of 4 ng/ml is considered to be reasonable by 47% of the involved GP-IS, whereas 16% prefer an age-adjusted PSA cut-off (IQ4). Patients with pathological PSA levels were immediately referred to a board-certified urologist by 69% of the GP-IS. On the other hand, 10% first would independently control elevated PSA levels themselves after 3-12 months (IQ5). Furthermore, 14% of the interviewed physicians consider a decrease of PCA-specific mortality by PSA screening as being proven (IQ6). Knowledge regarding PCA diagnostics is mainly based on continuous medical education for GP-IS (33%), personal contact with urologists (6%), and guideline studies (4%). While 53% indicated more than one education source, 4% did not obtain any PCA-specific training. The results provided by this questionnaire evaluating response of German GP-IS to six selected indicator questions fit well into the international context; however, further studies with sufficient methodical quality are required. CONCLUSIONS: Despite current findings and controversial recommendations of the two large PCA screening studies on this issue, German GP-IS still frequently use PCA screening by PSA measurement. Primary strategies of early detection as well as follow-up after assessment of pathologically elevated PSA levels poorly follow international recommendations. Thus, an intensification of specific education is justified.


Assuntos
Biomarcadores Tumorais/sangue , Diagnóstico Precoce , Antígeno Prostático Específico/sangue , Neoplasias da Próstata/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Comportamento Cooperativo , Comparação Transcultural , Detecção Precoce de Câncer , Medicina Geral , Alemanha , Humanos , Comunicação Interdisciplinar , Medicina Interna , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Valor Preditivo dos Testes , Neoplasias da Próstata/mortalidade , Neoplasias da Próstata/patologia , Inquéritos e Questionários , Taxa de Sobrevida
2.
Plant Biol (Stuttg) ; 15(4): 754-8, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23121076

RESUMO

Reduction of flux through photorespiration has been viewed as a major way to improve crop carbon fixation and yield since the energy-consuming reactions associated with this pathway were discovered. This view has been supported by the biomasses increases observed in model species that expressed artificial bypass reactions to photorespiration. Here, we present an overview about the major current attempts to reduce photorespiratory losses in crop species and provide suggestions for future research priorities.


Assuntos
Produtos Agrícolas/genética , Engenharia Genética , Plantas/genética , Biomassa , Ciclo do Carbono , Dióxido de Carbono/metabolismo , Respiração Celular , Cloroplastos/metabolismo , Produtos Agrícolas/metabolismo , Produtos Agrícolas/efeitos da radiação , Luz , Mitocôndrias/metabolismo , Fotossíntese , Plantas/metabolismo , Plantas/efeitos da radiação , Plantas Geneticamente Modificadas
3.
Mol Genet Genomics ; 269(6): 723-31, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12898218

RESUMO

To identify regions of the mitochondrial genome potentially involved in the expression of alloplasmic 'Tournefortii-Stiewe' cytoplasmic male sterility (CMS) in Brassica napus, transcripts of 25 mitochondrial genes were analysed in fertile and near isogenic male-sterile plants (BC(8) generation). Differences were detected in the transcription of genes for subunit 9 of ATP synthase (atp9), cytochrome b (cob) and subunit 2 of NADH dehydrogenase (nad2). Structural analysis of these gene regions revealed differences in genome organisation around atp9 between male-sterile and fertile plants. Three atp9 genes, two of which were hitherto unknown, are present in the mitochondria of CMS plants, and rearrangements upstream of one of these genes have generated a chimeric 193-codon ORF, designated orf193. This region is transcribed as a CMS specific bi-cistronic mRNA of 1.58 kb comprising orf193 and atp9. The level of the aberrant 1.58-kb transcript is reduced in plants restored to fertility by as yet uncharacterized nuclear genes. orf193 encodes a polypeptide of 22.7 kDa which exhibits partial sequence identity to the subunit 6 of the ATP synthase complex. However, as it forms an uninterrupted ORF with one of the newly discovered atp9 genes it may also be translated as a chimeric 30.2-kDa protein. It is likely that either or both gene products interfere with the function or assembly of the mitochondrial F(0)F(1)-ATP synthase, thus impairing the highly ATP-dependent process of pollen development. The novel molecular features of alloplasmic 'Tournefortii-Stiewe' CMS are discussed with respect to the other known mechanisms of CMS in B. napus.


Assuntos
Brassica napus/genética , Citoplasma/genética , DNA Mitocondrial/genética , Infertilidade/genética , Mitocôndrias/genética , ATPases Mitocondriais Próton-Translocadoras/genética , Proteínas de Plantas/genética , Proteolipídeos/genética , Apoproteínas/genética , Proteínas de Arabidopsis , Northern Blotting , Southern Blotting , Brassica napus/enzimologia , Grupo dos Citocromos b/genética , Citocromos b , Primers do DNA/química , Sondas de DNA , DNA de Plantas , Ordem dos Genes , Fases de Leitura Aberta , Reação em Cadeia da Polimerase , ATPases Translocadoras de Prótons , RNA de Plantas/genética , Mapeamento por Restrição , Transcrição Gênica
4.
Plant Physiol ; 127(4): 1694-710, 2001 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11743114

RESUMO

An Arabidopsis mitochondrial proteome project was started for a comprehensive investigation of mitochondrial functions in plants. Mitochondria were prepared from Arabidopsis stems and leaves or from Arabidopsis suspension cell cultures, and the purity of the generated fractions was tested by the resolution of organellar protein complexes applying two-dimensional blue-native/N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine (Tricine) sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The Arabidopsis mitochondrial proteome was analyzed by two-dimensional isoelectric focusing/ Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 650 different proteins in a pI range of pH 3 to 10 were separated on single gels. Solubilization conditions, pH gradients for isoelectric focusing, and gel staining procedures were varied, and the number of separable proteins increased to about 800. Fifty-two protein spots were identified by immunoblotting, direct protein sequencing, and mass spectrometry. The characterized proteins cooperate in various processes, such as respiration, citric acid cycle, amino acid and nucleotide metabolism, protection against O(2), mitochondrial assembly, molecular transport, and protein biosynthesis. More than 20% of the identified proteins were not described previously for plant mitochondria, indicating novel mitochondrial functions. The map of the Arabidopsis mitochondrial proteome should be useful for the analysis of knockout mutants concerning nuclear-encoded mitochondrial genes. Considerations of the total complexity of the Arabidopsis mitochondrial proteome are discussed. The data from this investigation will be made available at http://www.gartenbau.uni-hannover.de/genetik/AMPP.


Assuntos
Proteínas de Arabidopsis/isolamento & purificação , Arabidopsis/genética , Mitocôndrias/metabolismo , Proteoma/isolamento & purificação , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Técnicas de Cultura de Células , Eletroforese em Gel Bidimensional , Concentração de Íons de Hidrogênio , Internet , Caules de Planta/genética , Caules de Planta/metabolismo , Proteoma/metabolismo
5.
Plant J ; 28(1): 41-50, 2001 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11696185

RESUMO

Protoporphyrin IX is the last common intermediate of tetrapyrrole biosynthesis. The chelation of a Mg2+ ion by magnesium chelatase and of a ferrous ion by ferrochelatase directs protoporphyrin IX towards the formation of chlorophyll and heme, respectively. A full length cDNA clone encoding a ferrochelatase was identified from a Nicotiana tabacum cDNA library. The encoded protein consists of 497 amino acid residues with a molecular weight of 55.4 kDa. In vitro import of the protein into chloroplasts and its location in stroma and thylakoids confirm its close relationship to the previously described Arabidopsis thaliana plastid-located ferrochelatase (FeChII). A 1700-bp tobacco FeCh cDNA sequence was expressed in Nicotiana tabacum cv. Samsun NN under the control of the CaMV 35S promoter in antisense orientation allowing investigation into the consequences of selective reduction of the plastidic ferrochelatase activity for protoporphyrin IX channeling in chloroplasts and for interactions between plastidic and mitochondrial heme synthesis. Leaves of several transformants showed a reduced chlorophyll content and, during development, a light intensity-dependent formation of necrotic leaf lesions. In comparison with wild-type plants the total ferrochelatase activity was decreased in transgenic lines leading to an accumulation of photosensitizing protoporphyrin IX. Ferrochelatase activity was reduced only in plastids but not in mitochondria of transgenic plants. By means of the specifically diminished ferrochelatase activity consequences of the selective inhibition of protoheme formation for the intracellular supply of heme can be investigated in the future.


Assuntos
Ferroquelatase/biossíntese , Regulação da Expressão Gênica de Plantas , Nicotiana/citologia , Nicotiana/enzimologia , Plastídeos/enzimologia , RNA Antissenso/metabolismo , Clonagem Molecular , Ferroquelatase/genética , Ferroquelatase/metabolismo , Regulação Enzimológica da Expressão Gênica , Heme/metabolismo , Isoenzimas/biossíntese , Isoenzimas/genética , Isoenzimas/metabolismo , Luz , Mitocôndrias/enzimologia , Necrose , Fenótipo , Filogenia , Plantas Geneticamente Modificadas , Plastídeos/genética , Plastídeos/metabolismo , Plastídeos/efeitos da radiação , Protoporfirinas/metabolismo , RNA Antissenso/biossíntese , RNA Antissenso/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA de Plantas/genética , RNA de Plantas/metabolismo , Nicotiana/genética , Nicotiana/metabolismo
6.
Indian J Biochem Biophys ; 37(1): 59-66, 2000 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10983414

RESUMO

Purification of mitochondria and mitochondrial protein complexes from green tissues is often severely impaired by the presence of chloroplasts and their proteins. Here we present a method which allows analysis of respiratory protein complexes from potato leaves. The procedure includes the preparation of an organellar fraction specifically enriched in mitochondria and the separation of organellar protein complexes by blue-native polyacrylamide gel electrophoresis (BN-PAGE). For the first time mitochondrial and chloroplast protein complexes have been resolved simultaneously in a native gel. BN-PAGE allowed the separation of eleven bands, including the mitochondrial NADH-dehydrogenase, the bc1 complex and the mitochondrial F1-ATP synthase as well as the chloroplast F1-ATP synthase, the cytochrome b6f complex, the two photosystems and the light harvesting complex. The resolution of the protein complexes in the first dimension was good enough to allow identification of all subunits of individual complexes in the second dimension under denaturing conditions. Thus, BN-PAGE offers an opportunity to analyze mitochondrial and chloroplast protein complexes from a single preparation from very small amounts of tissue. The implications of our findings, for studies on protein expression and turnover in different tissues and developmental stages, are discussed.


Assuntos
Proteínas de Plantas/isolamento & purificação , Cloroplastos/química , Eletroforese em Gel Bidimensional , Proteínas de Membrana/química , Proteínas de Membrana/isolamento & purificação , Mitocôndrias/química , Folhas de Planta/química , Proteínas de Plantas/química , Solanum tuberosum/química
7.
Planta ; 209(3): 267-74, 1999 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10502092

RESUMO

The import and assembly of mitochondrial proteins synthesized in the cytosol is mediated by the protein-import apparatus which plays a central role in mitochondrial biogenesis. Ten years ago only some components of the protein-import apparatus from fungi and mammals were characterized, but today its major components have been analyzed at the molecular level also in plants. Interestingly there are specific features which distinguish the protein-import apparatus of plants from that of fungi and mammals. Here we give an overview of all known components of the protein-import apparatus from plants and focus on its differences in comparison to heterotrophic eukaryotes.


Assuntos
Mitocôndrias/metabolismo , Proteínas de Plantas/metabolismo , Plantas/metabolismo , Animais , Transporte Biológico
8.
Urologe A ; 37(4): 382-5, 1998 Jul.
Artigo em Alemão | MEDLINE | ID: mdl-9738290

RESUMO

In total malignant mixed tumors (carcinosarcomas) represent a very rare gynecological neoplasm. In accordance with the embryological genesis primary localizations are with descending probability the uterus, the ovarians and finally the tubes. We report on a 55 year old female patient, with a paraurethral mass, which was primarily diagnosed in 1994. Histopathological evaluation of a transvaginal biopsy did not demonstrate evidence of malignancy at that time. After painful enlargement the paraurethral mass was resected surgically and histopathological evaluation revealed a primary paraurethral malignant mixed tumor (carcinosarcoma). Postoperatively, the patient underwent percutaneous (46.4 Gy) and intracavitary (2 x 6 Gy) radiation. Twelve months postoperatively there is no evidence of disease.


Assuntos
Carcinossarcoma/diagnóstico , Tumor Misto Maligno/diagnóstico , Neoplasias Uretrais/diagnóstico , Neoplasias Vaginais/diagnóstico , Braquiterapia , Carcinossarcoma/patologia , Carcinossarcoma/radioterapia , Carcinossarcoma/cirurgia , Terapia Combinada , Feminino , Humanos , Pessoa de Meia-Idade , Tumor Misto Maligno/patologia , Tumor Misto Maligno/radioterapia , Tumor Misto Maligno/cirurgia , Radioterapia Adjuvante , Uretra/patologia , Uretra/cirurgia , Neoplasias Uretrais/patologia , Neoplasias Uretrais/radioterapia , Neoplasias Uretrais/cirurgia , Vagina/patologia , Vagina/cirurgia , Neoplasias Vaginais/patologia , Neoplasias Vaginais/radioterapia , Neoplasias Vaginais/cirurgia
9.
J Biol Chem ; 273(27): 17251-7, 1998 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-9642296

RESUMO

Transport of most nuclear encoded mitochondrial proteins into mitochondria is mediated by heteropolymeric translocases in the membranes of the organelles. The translocase of the outer mitochondrial membrane (TOM) was characterized in fungi, and it was shown that TOM from yeast comprises nine different subunits. This publication is the first report on the preparation of the TOM complex from plant mitochondria. The protein complex from potato was purified by (a) blue native polyacrylamide gel electrophoresis and (b) by immunoaffinity chromatography. On blue native gels, the potato TOM complex runs close to cytochrome c oxidase at 230 kDa and hence only comprises about half of the size of fungal TOM complexes. Analysis of the TOM complex from potato by SDS-polyacrylamide gel electrophoresis allows separation of seven different subunits of 70, 36, 23, 9, 8, 7, and 6 kDa. The 23-kDa protein is identical to the previously characterized potato TOM20 receptor, as shown by in vitro assembly of this protein into the 230-kDa complex, by immunoblotting and by direct protein sequencing. Partial amino acid sequence data of the other subunits allowed us to identify sequence similarity between the 36-kDa protein and fungal TOM40. Sequence analysis of cDNAs encoding the 7-kDa protein revealed significant sequence homology of this protein to TOM7 from yeast. However, potato TOM7 has a N-terminal extension, which is very rich in basic amino acids. Counterparts to the TOM22 and TOM37 proteins from yeast seem to be absent in the potato TOM complex, whereas an additional low molecular mass subunit occurs. Functional implications of these findings are discussed.


Assuntos
Adenosina Trifosfatases/metabolismo , Proteínas de Bactérias/metabolismo , Proteínas de Escherichia coli , Proteínas de Membrana Transportadoras , Mitocôndrias/enzimologia , Solanum tuberosum/enzimologia , Adenosina Trifosfatases/genética , Adenosina Trifosfatases/isolamento & purificação , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Sequência de Bases , Cromatografia de Afinidade/métodos , Clonagem Molecular , Eletroforese em Gel de Poliacrilamida , Membranas Intracelulares/enzimologia , Dados de Sequência Molecular , Peso Molecular , Canais de Translocação SEC , Proteínas SecA , Homologia de Sequência de Aminoácidos
10.
J Biol Chem ; 273(21): 13143-9, 1998 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-9582354

RESUMO

The mitochondrial processing peptidase (MPP) is a heterodimeric enzyme that forms part of the cytochrome c reductase complex from higher plants. Mitochondria from mammals and yeast contain two homologous enzymes: (i) an active MPP within the mitochondrial matrix and (ii) an inactive MPP within the cytochrome c reductase complex. To elucidate the evolution of MPP, the cytochrome c reductase complexes from lower plants were isolated and tested for processing activity. Mitochondria were prepared from the staghorn fern Platycerium bifurcatum, from the horsetail Equisetum arvense, and from the colorless algae Polytomella, and cytochrome c reductase complexes were purified by a micro-isolation procedure based on Blue-native polyacrylamide gel electrophoresis and electroelution. This is the first report on the subunit composition of a respiratory enzyme complex from a fern or a horsetail. The cytochrome c reductase complexes from P. bifurcatum and E. arvense are shown to efficiently process mitochondrial precursor proteins, whereas the enzyme complex from Polytomella lacks proteolytic activity. An evolutionary model is suggested that assumes a correlation between the presence of an active MPP within the cytochrome c reductase complex and the occurrence of chloroplasts.


Assuntos
Evolução Molecular , Metaloendopeptidases/genética , NADH Desidrogenase/genética , Sequência de Aminoácidos , Animais , Eletroforese em Gel Bidimensional , Humanos , Metaloendopeptidases/química , Metaloendopeptidases/metabolismo , Mitocôndrias/enzimologia , Dados de Sequência Molecular , NADH Desidrogenase/química , NADH Desidrogenase/metabolismo , Plantas/ultraestrutura , Processamento de Proteína Pós-Traducional , Homologia de Sequência de Aminoácidos , Peptidase de Processamento Mitocondrial
11.
Int J Biochem Cell Biol ; 29(8-9): 1043-5, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9415998

RESUMO

The mitochondrial processing peptidase (MPP) is a heterodimeric enzyme which plays an essential role in mitochondrial protein import. It cleaves off the N-terminal targeting signals of nuclear encoded mitochondrial proteins upon their transport into the organelle. In mammals and yeast the enzyme is localized in the mitochondrial matrix while in plants it is integrated into a protein complex of the respiratory chain. As the activity of MPP is essential for the viability of eukaryotic cells it is conceivable that inhibitors of MPP which are specific for the soluble enzyme only present in fungi and animals may work as fungicides or insecticides.


Assuntos
Metaloendopeptidases , Animais , Transporte Biológico , Dimerização , Metaloendopeptidases/biossíntese , Metaloendopeptidases/química , Metaloendopeptidases/metabolismo , Metaloendopeptidases/fisiologia , Mitocôndrias/metabolismo , Neurospora/enzimologia , Plantas/enzimologia , Sinais Direcionadores de Proteínas/metabolismo , Peptidase de Processamento Mitocondrial
12.
Planta ; 201(3): 326-33, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9129338

RESUMO

The 23-kDa nuclear-encoded heat-shock protein (HSP) of Chenopodium rubrum L. is regulated by light at the posttranslational level. Higher light intensities are more effective in inducing the accumulation of the mature protein under heat-shock conditions. Based on this and other properties the protein was considered to belong to the group of small chloroplastic HSPs. However, we have now obtained the following evidence that this 23-kDa HSP is localized in the mitochondria: (i) Immunogold-labelled protein was almost exclusively restricted to the mitochondria in electron microscope thin sections. (ii) Using purified, isolated mitochondria from potato tubers the in-vitro-synthesized translation product of 31 kDa was readily transported into mitochondria where it was processed to the 23-kDa product. (iii) The protein could be detected by Western blotting in a preparation of washed mitochondria of Chenopodium, while under the same conditions no signal could be obtained in a preparation of isolated chloroplasts. (iv) Finally, sequence comparison with the published sequences of mitochondrial proteins by Lenne et al. (1995, Biochem J 311:805-813) and LaFayette et al. (1996, Plant Mol Biol 30:159-169) showed clearly that the 23-kDa protein is considerably more similar to these two proteins than to the group of plastid small HSPs. From these data we infer that mitochondria are involved in the response of the plants to high light stress under heat-shock conditions.


Assuntos
Proteínas de Choque Térmico/biossíntese , Mitocôndrias/metabolismo , Proteínas de Plantas/biossíntese , Plantas/metabolismo , Sequência de Aminoácidos , Fracionamento Celular , Núcleo Celular/metabolismo , Células Cultivadas , Proteínas de Drosophila , Proteínas de Choque Térmico/análise , Proteínas de Choque Térmico/química , Temperatura Alta , Proteínas de Membrana/análise , Proteínas de Membrana/biossíntese , Microscopia Eletrônica , Mitocôndrias/ultraestrutura , Dados de Sequência Molecular , Peso Molecular , Proteínas de Plantas/análise , Plastídeos/metabolismo , Plastídeos/ultraestrutura , Biossíntese de Proteínas , Homologia de Sequência de Aminoácidos , Transcrição Gênica
13.
Biochem J ; 320 ( Pt 3): 769-75, 1996 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-9003361

RESUMO

cDNA clones encoding subunit VII of the Neurospora crassa bc1 complex (ubiquinol:cytochrome-c oxidoreductase), which is homologous with subunit VIII of the complex from yeast (encoded by QCR8), were identified on the basis of functional complementation of a yeast QCR8 deletion strain. The clones contain an open reading frame encoding a protein with a calculated molecular mass of 11.8 kDa. The N-terminal eight residues of the amino acid sequence deduced from the cDNA clones are absent from the mature protein, as revealed by direct sequencing of the isolated protein. To investigate the potential role of the N-terminal octapeptide in mitochondrial targeting, constructs were made encoding the precursor and the mature form of subunit VII from Neurospora. Incubation of isolated mitochondria with the two proteins revealed that the N-terminal extension of the precursor is removed on import. However, the presequence does not encode information for targeting, as the proteins encoded by both constructs can be imported into isolated mitochondria with equal efficiency. In contrast, the octapeptide seems to have functional importance: the defect in the yeast qcr8-null mutant is not complemented on transformation with the construct encoding mature subunit VII from N. crassa in a single-copy plasmid. We therefore speculate that the N-terminal extension plays a role in intramitochondrial sorting of N. crassa subunit VII. This is supported by the fact that the subunit VII precursor is processed by a protease other than the general mitochondrial processing peptidase. Interestingly, the presequence of N. crassa subunit VII has an amino acid composition similar to the octapeptides cleaved off by the mitochondrial intermediate peptidase.


Assuntos
Complexo III da Cadeia de Transporte de Elétrons/química , Mitocôndrias/metabolismo , Neurospora crassa/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Divisão Celular/genética , Clonagem Molecular , Complexo III da Cadeia de Transporte de Elétrons/genética , Complexo III da Cadeia de Transporte de Elétrons/metabolismo , Eletroforese em Gel de Poliacrilamida , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Metaloendopeptidases/metabolismo , Dados de Sequência Molecular , Peso Molecular , Plasmídeos/genética , Alinhamento de Sequência , Análise de Sequência , Transformação Genética/genética , Peptidase de Processamento Mitocondrial
14.
Plant J ; 9(3): 357-68, 1996 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-8919912

RESUMO

Recently a powerful electrophoresis method for the native preparation and characterization of the respiratory protein complexes of mitochondria from fungi and mammals has been developed, which employs Coomassie dyes to introduce charge shifts on proteins (Schägger and von Jagow (1991) Anal. Biochem. 199, 223-231). The procedure, which is called 'blue native-polyacrylamide gel electrophoresis' (BN-PAGE), was modified and introduced for the analysis of mitochondria from higher plants. BN-PAGE of mitochondrial protein from potato allows the separation of nine distinct protein complexes between 100 and 1000 kDa and reveals novel results for their composition, molecular mass and stoichiometry. For the first time soluble mitochondrial protein complexes, like the HSP60 complex (750 kDa) and a complex of 200 kDa, which includes a formate dehydrogenase, are analysed by BN-PAGE. Complex I from potato (1000 kDa) is about 100 kDa larger than the corresponding enzyme from beef and can be resolved into more than 30 different subunits on a second gel dimension. The F1F0 ATP synthase (580 kDa) and the cytochrome c oxidase (160 kDa) from potato seem to contain more subunits than hitherto reported. Direct sequencing of subunits revealed that the F1 part of the F1F0 ATP synthase lacks the oligomycin sensitivity conferring protein (OSCP), which was reported to be present in F1 parts of dicotyledonous plants, but contains the ATPase inhibitory protein. N-terminal sequences of 16 mitochondrial proteins were obtained, several of which are presented for the first time from a plant source. BN-PAGE allows the preparation of mitochondrial protein complexes from gram amounts of plant tissue, as the procedure only requires milligram amounts of organelles. This potential of BN-PAGE is demonstrated by the separation and characterization of the mitochondrial enzyme complexes from Arabidopsis thaliana. Further analysis of organellar protein complexes by BN-PAGE will allow the generation of 'protein maps' from different tissues and developmental stages or from mutant plants.


Assuntos
Mitocôndrias/química , Proteínas de Plantas/química , Sequência de Aminoácidos , Arabidopsis/química , Arabidopsis/enzimologia , Arabidopsis/genética , Eletroforese em Gel de Poliacrilamida/métodos , Dados de Sequência Molecular , Peso Molecular , Proteínas de Plantas/genética , Proteínas de Plantas/isolamento & purificação , Conformação Proteica , Desnaturação Proteica , ATPases Translocadoras de Prótons/química , ATPases Translocadoras de Prótons/genética , ATPases Translocadoras de Prótons/isolamento & purificação , Solanum tuberosum/química , Solanum tuberosum/genética , Solubilidade
15.
Mol Biol Rep ; 23(2): 71-7, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-8983020

RESUMO

Large-scale random cDNA sequencing projects have been started for several organisms and are a valuable tool for the analysis of quantitative and qualitative aspects of gene expression. However, the reliability of the obtained data is limited as most of the clones are only partially analysed on one strand. As a consequence the sequence entries derived from random cDNA sequencing projects usually comprise incomplete open reading frames. They nevertheless define complete and reliable coding sequences, if two prerequisites are fulfilled: (i) the clones encode very small proteins, and (ii) the clones have a high frequency in the cDNA-banks. The present study describes the use of cDNA databases for the identification of homologues of three low-molecular-weight subunits of the mitochondrial bc1 complex, termed the QCR6, QCR9 and QCR10 proteins. These polypeptides are only characterized for a small number of organisms, have a scarcely defined function and exhibit a low degree of structural conservation if compared between different species. Several clones were identified for each polypeptide by searches with TBLASTN using the known sequences as probes. Most of the database entries contain complete open reading frames and sequencing queries could be excluded due to the abundancy of the clones. Multiple sequence alignments are presented for all three polypeptides and consensus sequences are given which may provide a basis for the investigation of the proteins by site-directed mutagenesis.


Assuntos
Complexo III da Cadeia de Transporte de Elétrons/química , Proteínas Nucleares , Proteínas de Saccharomyces cerevisiae , Animais , Sequência de Bases , Sequência Consenso , DNA Complementar/genética , Complexo III da Cadeia de Transporte de Elétrons/genética , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Expressão Gênica , Humanos , Dados de Sequência Molecular , Peso Molecular , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas/genética , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos
16.
J Bioenerg Biomembr ; 27(4): 423-36, 1995 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-8595978

RESUMO

Cytochrome c reductase from potato has been extensively studied with respect to its catalytic activities, its subunit composition, and the biogenesis of individual subunits. Molecular characterization of all 10 subunits revealed that the high-molecular-weight subunits exhibit striking homologies with the components of the general mitochondrial processing peptidase (MPP) from fungi and mammals. Some of the other subunits show differences in the structure of their targeting signals or in their molecular composition when compared to their counterparts from heterotrophic organisms. The proteolytic activity of MPP was found in the cytochrome c reductase complexes from potato, spinach, and wheat, suggesting that the integration of the protease into this respiratory complex is a general feature of higher plants.


Assuntos
Precursores Enzimáticos/química , Metaloendopeptidases/química , Metaloendopeptidases/metabolismo , Mitocôndrias/enzimologia , NADH Desidrogenase/química , NADH Desidrogenase/metabolismo , Solanum tuberosum/enzimologia , Sequência de Aminoácidos , Precursores Enzimáticos/metabolismo , Cinética , Substâncias Macromoleculares , Metaloendopeptidases/biossíntese , Dados de Sequência Molecular , NADH Desidrogenase/biossíntese , Plantas/enzimologia , Homologia de Sequência de Aminoácidos , Especificidade da Espécie , Peptidase de Processamento Mitocondrial
17.
Trends Biochem Sci ; 20(5): 171-5, 1995 May.
Artigo em Inglês | MEDLINE | ID: mdl-7610476

RESUMO

The so-called 'core' proteins of the respiratory cytochrome bc1 complex and the two subunits of the mitochondrial processing peptidase (MPP) are structurally similar but their evolutionary relationship remains a mystery. Here, we present a model suggesting that the core proteins originated from an ancient proteolytic enzyme that was integrated into the bc1 complex during early stages of endosymbiosis.


Assuntos
Evolução Biológica , Metaloendopeptidases/química , NADH Desidrogenase/química , Sequência de Aminoácidos , Animais , Humanos , Dados de Sequência Molecular , Peptidase de Processamento Mitocondrial
18.
Biochim Biophys Acta ; 1229(2): 181-6, 1995 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-7727497

RESUMO

The cytochrome-c reductase (EC 1.10.2.2) of the mitochondrial respiratory chain couples electron transport from ubiquinol to cytochrome c with proton translocation across the inner mitochondrial membrane. The enzyme from potato was shown to be composed of 10 subunits. Isolation and characterization of cDNA clones for the second smallest subunit reveal an open reading frame of 216 bp encoding a protein of 8.0 kDa. The protein exhibits similarities to a 7.2/7.3 kDa subunit of cytochrome-c reductase from bovine and yeast, that is localized on the intermembrane space side of the enzyme complex. It also shows similarity to a previously unidentified 7.8 kDa protein of cytochrome-c reductase from Euglena. The potato 8.0 kDa protein has a segmental structure, as its sequence can be divided into four parts, each comprising a central Arg-(Xaa)5-Val motif. N-terminal sequencing of the mature 8.0 kDa proteins indicates the absence of a cleavable mitochondrial targeting sequence. Import of the in vitro synthesized 8.0 kDa protein into isolated potato mitochondria confirms the lack of a presequence and reveals a dependence of the transport on the membrane potential delta psi across the inner mitochondrial membrane. These features are unique among the intermembrane space proteins known so far.


Assuntos
NADH Desidrogenase/química , Solanum tuberosum/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Transporte Biológico , Clonagem Molecular , DNA Complementar , DNA de Plantas , Potenciais da Membrana , Dados de Sequência Molecular , NADH Desidrogenase/genética , NADH Desidrogenase/metabolismo , Homologia de Sequência de Aminoácidos
19.
Plant Physiol ; 107(4): 1217-23, 1995 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-7770525

RESUMO

The cytochrome c reductase complexes from fungi and mammals both contain a 14-kD protein (yeast, 14.4 kD; bovine, 13.4 kD) that does not directly participate in electron transfer but possibly is indirectly involved in the function of the complex and has a role in assembly of the multimeric enzyme. A subunit of comparable size was identified for the bc1 complex of higher plants. The 14-kD protein from potato (Solanum tuberosum) was specifically separated from the isolated protein complex in the presence of 6 M urea and is, therefore, assumed to be a peripheral component. Direct sequence analysis of the proteins from potato and wheat (Triticum aestivum) and isolation of corresponding cDNA clones for the subunit from potato revealed clear similarity to the equivalent proteins from yeast and bovine. The wheat 14-kD protein seems to occur in two isoforms. The 14-kD protein from plants is very hydrophilic, has a characteristic charge distribution, and contains no potential membrane-spanning helices. In vitro import of the radiolabeled 14-kD protein from potato into isolated mitochondria depends on the membrane potential across the inner mitochondrial membrane. The protein seems to lack a cleavable mitochondrial presequence, because it is not processed upon translocation. Possible intramolecular regions involved in targeting of the 14-kD protein to plant mitochondria are discussed.


Assuntos
NADH Desidrogenase/genética , Solanum tuberosum/enzimologia , Solanum tuberosum/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Bovinos , DNA de Plantas/genética , Mitocôndrias/enzimologia , Dados de Sequência Molecular , Peso Molecular , NADH Desidrogenase/química , NADH Desidrogenase/metabolismo , Conformação Proteica , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/genética , Homologia de Sequência de Aminoácidos , Especificidade da Espécie , Triticum/enzimologia , Triticum/genética
20.
Eur J Biochem ; 228(3): 878-85, 1995 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-7737189

RESUMO

Analysis of cytochrome c reductase from potato by Tricine/SDS/PAGE reveals 10 bands representing 10 different subunits. In comparison to glycine/SDS/PAGE one additional small protein becomes visible, whereas the three large core proteins are not resolved. The identity of the subunits was determined by immunoblotting and direct sequence determination. Sequence data for the novel small component were used to derive oligonucleotides for probing a potato cDNA-library and isolating corresponding clones. The newly identified subunit is a 6.7-kDa protein, that exhibits significant sequence similarity to a 8.5-kDa subunit of cytochrome c reductase from yeast and the 6.5-kDa iron-sulfur-protein-binding factor from the equivalent enzyme complex from beef. Also the potato 6.7-kDa subunit can be dissociated from the cytochrome c reductase complex together with the iron-sulfur protein. To address the question of whether three or two core subunits occur simultaneously in monomeric cytochrome c reductase complexes from potato, a peptide-specific antibody was generated. The antiserum is capable of discriminating between the 55-kDa and 53-kDa core proteins, which can be separated by glycine/SDS/PAGE and which were previously found to be structurally related. Immunoprecipitations of isolated cytochrome c reductase from potato using this antibody revealed an enzyme complex containing only two core proteins. The simultaneous occurrence of only two core subunits was confirmed by a comparison of the molecular masses of cytochrome c reductase from potato and beef by blue-native-gel electrophoresis. Hence the cytochrome c reductase complexes from potato, beef and yeast have a very conserved subunit composition. The evolutionary implications of these findings are discussed.


Assuntos
NADH Desidrogenase/química , Solanum tuberosum/enzimologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Western Blotting , Bovinos , Clonagem Molecular , DNA Complementar , Eletroforese em Gel de Poliacrilamida , Carne , Mitocôndrias/enzimologia , Dados de Sequência Molecular , Peso Molecular , NADH Desidrogenase/genética , Testes de Precipitina , Saccharomyces cerevisiae/enzimologia , Homologia de Sequência de Aminoácidos
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