Maternal transmission as a microbial symbiont sieve, and the absence of lactation in male mammals.

Gut microbiomes of mammals carry a complex symbiotic assemblage of microorganisms. Feeding newborn infants milk from the mammary gland allows vertical transmission of the parental milk microbiome to the offspring's gut microbiome. This has benefits, but also has hazards for the host population. Using mathematical models, we demonstrate that biparental vertical transmission enables deleterious microbial elements to invade host populations. In contrast, uniparental vertical transmission acts as a sieve, preventing these invasions. Moreover, we show that deleterious symbionts generate selection on host modifier genes that keep uniparental transmission in place. Since microbial transmission occurs during birth in placental mammals, subsequent transmission of the milk microbiome needs to be maternal to avoid the spread of deleterious elements. This paper therefore argues that viviparity and the hazards from biparental transmission of the milk microbiome, together generate selection against male lactation in placental mammals.

Electrical synapse structure requires distinct isoforms of a postsynaptic scaffold.

Electrical synapses are neuronal gap junction (GJ) channels associated with a macromolecular complex called the electrical synapse density (ESD), which regulates development and dynamically modifies electrical transmission. However, the proteomic makeup and molecular mechanisms utilized by the ESD that direct electrical synapse formation are not well understood. Using the Mauthner cell of zebrafish as a model, we previously found that the intracellular scaffolding protein ZO1b is a member of the ESD, localizing postsynaptically, where it is required for GJ channel localization, electrical communication, neural network function, and behavior. Here, we show that the complexity of the ESD is further diversified by the genomic structure of the ZO1b gene locus. The ZO1b gene is alternatively initiated at three transcriptional start sites resulting in isoforms with unique N-termini that we call ZO1b-Alpha, -Beta, and -Gamma. We demonstrate that ZO1b-Beta and ZO1b-Gamma are broadly expressed throughout the nervous system and localize to electrical synapses. By contrast, ZO1b-Alpha is expressed mainly non-neuronally and is not found at synapses. We generate mutants in all individual isoforms, as well as double mutant combinations in cis on individual chromosomes, and find that ZO1b-Beta is necessary and sufficient for robust GJ channel localization. ZO1b-Gamma, despite its localization to the synapse, plays an auxiliary role in channel localization. This study expands the notion of molecular complexity at the ESD, revealing that an individual genomic locus can contribute distinct isoforms to the macromolecular complex at electrical synapses. Further, independent scaffold isoforms have differential contributions to developmental assembly of the interneuronal GJ channels. We propose that ESD molecular complexity arises both from the diversity of unique genes and from distinct isoforms encoded by single genes. Overall, ESD proteomic diversity is expected to have critical impacts on the development, structure, function, and plasticity of electrical transmission.

Concentrated Learning Experiences Across Two Different Health-Systems.

BACKGROUND: While many public pharmacy schools have an adjoining health-system to accommodate their students, some pharmacy programs form partnerships with non-affiliated health-systems to precept students. These health-systems often afford students the opportunity to complete multiple rotations within a single organization, offering decreased onboarding time and more longitudinal experiences. INNOVATION: Two autonomous partnerships were developed with independent healthcare systems for concentrated learning experiences during the advanced pharmacy practice experiences year. Each program differs in student requirements and is overseen by the practice site, with participation by area faculty. KEY FINDINGS: A survey assessed professional skill set development, achievement of program goals during the experiential year, and student satisfaction of a concentrated learning experience. A comparison between programs was completed to assess for consistency in student experiences. Nineteen students (83%) responded to the survey. Students from both health-systems reported similarities in professional skill growth. Likewise, all students reported achievement of program goals and overall satisfaction with their experiential training. CONCLUSIONS: Independently managed concentrated learning experiences provided evidence of consistent growth in student professional development and achievement of programmatic goals. Partnerships with non-affiliated healthcare systems can provide a rich training ground for student learners.

BrachyView: development of an algorithm for real-time automatic LDR brachytherapy seed detection.

BrachyView is a novel in-body imaging system developed to provide real-time intraoperative dosimetry for low dose rate prostate brachytherapy treatments. Seed positions can be reconstructed after in-vivo implantation using a high-resolution pinhole gamma camera inserted into the patient rectum. The obtained data is a set of 2D projections of the seeds on the image plane. The 3D reconstruction algorithm requires the identification of the seed's centre of mass. This work presents the development and techniques adopted to build an algorithm that provides the means for fully automatic seed centre of mass identification and 3D position reconstruction for real-time applications. The algorithm presented uses a local feature detector, speeded up robust features, to perform detection of brachytherapy seed 2D projections from images, allowing for robust seed identification. Initial results have been obtained with datasets of 30, 96 and 98 I-125 brachytherapy seeds implanted into a prostate gel phantom. It can detect 97% of seeds and correctly match 97% of seeds. The average overall computation time of 2.75 s per image and improved reconstruction accuracy of 22.87% for the 98 seed dataset was noted. Elimination processes for initial false positive detection removal have shown to be extremely effective, resulting in a 99.9% reduction of false positives, and when paired with automatic frame alignment and subtraction procedures allows for the effective removal of excess counts generated by previously implanted needles. The proposed algorithm will allow the BrachyView system to be used as a real-time intraoperative dosimetry tool for low dose rate prostate brachytherapy treatments.

BrachyView: Reconstruction of seed positions and volume of an LDR prostate brachytherapy patient plan using a baseline subtraction algorithm.

PURPOSE: BrachyView is a novel in-body imaging system developed with the objective to provide real-time intraoperative dosimetry for low dose rate (LDR) prostate brachytherapy treatments. The BrachyView coordinates combined with conventional transrectal ultrasound (TRUS) imaging, provides the possibility to localise the effective position of the implanted seeds inside the prostate volume, providing a unique tool for intra-operative verification of the quality of the implantation. This research presents the first complete LDR brachytherapy plan reconstructed by the BrachyView system and is used to evaluate the effectiveness of an imaging algorithm with baseline subtraction. METHODS: A plan featuring 98 I-125 brachytherapy seeds, with an average activity of 0.248 mCi, were implanted into a prostate gel phantom under TRUS guidance. Images of implanted seeds were obtained by the BrachyView after the implantation of seeds. The baseline subtraction algorithm is applied as a pixel-to-pixel counts subtraction and is applied to every second projection obtained after the implantation of each needle. Seed positions and effectiveness of the baseline reconstruction in the identification of seeds were verified by a high-resolution post-implant CT scan. RESULTS: A complete brachytherapy plan has been reconstructed with a 100% detection rate. This is possible due to the effectiveness of the baseline subtraction, with its application an overall increase of 11.3% in position accuracy and 8.2% increase in detection rate was noted. CONCLUSION: It has been demonstrated that the BrachyView system shows the potential to be a solution to providing clinics with the means for intraoperative dosimetry for LDR prostate brachytherapy treatments.

Patient Recall of Information on a Third Molar Informed Consent Video.

PURPOSE: Experience has shown that in malpractice cases patients often claim certain information was not provided during the informed consent process. The purposes of this study were to determine how much of an informed consent video patients initially remember and how much is recalled when there is a hiatus from viewing the video to the surgery. PATIENTS AND METHODS: In this prospective cohort study, patients presenting for impacted third molar removal in an academic setting (n = 50) or a private office (n = 50) viewed an informed consent video and then met with the treating doctor for further discussion and signing of an informed consent form. Then, they were given a 10-item test containing questions relating to the information provided on the video. At the subsequent time of surgery, they were again given the same test. The number of correct answers at the 2 points was compared. RESULTS: There was a significant difference in the number of correct responses between the 2 time points (P = .0212) and between patients from the 2 practice settings (P = .0076). However, these differences were less than 0.5 of a possible 10. The number of correct responses was not associated with age (P = .1203), gender (P = .6647), or number of days between testing (P = .7272). CONCLUSION: Patients have good recall of the information provided by the informed consent video. However, because some information was forgotten, a presurgical review of the information is advisable.

Face naming in dementia: a reply to Hodges and Greene (1998)

A case study by Brennen, David, Fluchaire, and Pellat (1996) reported the case of a patient who could occasionally name celebrities with very low concurrent levels of semantic access, which is difficult to reconcile with current models of face identification. Hodges and Greene (1998) attribute Brennen et al.'s case study to artifactual explanations and provide new data, which, they claim, is evidence against the "theory of naming without semantics". This reply demonstrates that Hodges and Greene's arguments are unconvincing and that aspects of Hodges and Greene's data in fact provide support for Brennen et al.'s conclusions. It is also argued that in cases of dementia direct naming has been reported for other stimuli domains as well.

Arctic cognition: a study of cognitive performance in summer and winter at 69 degrees N.

Evidence has accumulated over the past 15 years that affect in humans is cyclical. In winter there is a tendency to depression, with remission in summer, and this effect is stronger at higher latitudes. In order to determine whether human cognition is similarly rhythmical, this study investigated the cognitive processes of 100 participants living at 69 degrees N. Participants were tested in summer and winter on a range of cognitive tasks, including verbal memory, attention and simple reaction time tasks. The seasonally counterbalanced design and the very northerly latitude of this study provide optimal conditions for detecting impaired cognitive performance in winter, and the conclusion is negative: of five tasks with seasonal effects, four had disadvantages in summer. Like the menstrual cycle, the circannual cycle appears to influence mood but not cognition.

[Deficiency in the reproduction and learning proper names after left tubero-thalamic ischemic lesion]. / Déficit de la production et de l'apprentissage des noms propres après lésion ischémique tubéro-thalamique gauche.

Predominant impairment or preservation of category-specific naming and comprehension is not rare in aphasics. Much less frequent is a selective inability to generate proper names. To our knowledge, only one such case has been reported after a left thalamic lesion, located in the ventral anterior nucleus, the mamillo-thalamic tractus and the genu of the internal capsule. We report a new case of selective inability to generate proper names after a left tubero-thalamic infarct. A 65-year old right-handed man presented with a selective impairment in producing proper names, both from photographs or descriptions and on tests of verbal fluency. The deficit was obvious both for persons names and for geographical names. The rest of the neuropsychological testing was remarkable only for a mild verbal amnesia, affecting only serial material (list of words), a reduced fluency for flowers, fruits and musical instruments, difficulties in learning of new words, and a dissociation between preserved learning for words on a test of learning of words and occupations (Cohen, 1990). This anomia for proper names could result from an indirect frontal-lobe dysfunction, preventing voluntary activation of the phonological representation of proper names.

The difficulty with recalling people's names: the plausible phonology hypothesis.

Recalling the name of a person is a simple, but often a problematic, everyday task. There are various explanations of this phenomenon, but here it is argued that the explanations offered so far, by failing to consider learning of names, have overlooked a simple account of name recall difficulty. The starting observation for this viewpoint is that names of people are often non-words, in that they have never been encountered before. This is not true of, say, names of professions. Not only does the relatively high rate of new exemplars mean that people's names are likely to be underlearned, but furthermore, even for equal degrees of learning, a person's name is at a disadvantage because of the high plausibility of most phonologies: "dreaner" is much more readily accepted as the name of a person than as the name of their profession. So specifying the phonology of people's names is inherently a more demanding task, compared to the phonology of other names. The implications of this view are explored with regard to explanations of empirically established name recall phenomena in normal subjects, the patterns of performance of anomic patients and the difficulty of name recall in different word domains. It is shown that these arguments, derived from a real world fact, account in a simple way for existing data and make predictions in different areas of research.

Context effects in the processing of familiar faces.

In this paper we report five experiments that investigate the influence of prime faces upon the speed with which familiar faces are recognized and named. Previously, priming had been reported when the prime and target faces were closely associated, e.g., Prince Charles and Princess Diana (Bruce & Valentine, 1986). In Experiment 1 we show that there is a reliable effect of relatedness on a double-familiarity decision, even when the faces are only categorically related, e.g., Kirk Douglas and Clint Eastwood. Then it was shown that such an effect emerges only on a double decision task (Experiments 2 and 3). Experiment 4 showed that on a primed naming task, faces preceded by a categorically related prime were responded to more quickly than those preceded by an unrelated prime, and the effect was due to inhibition. Experiment 5 replicated this effect and also showed that when associatively related primes were used, a facilitatory, and not an inhibitory, effect is found. It is argued that the facilitation of associative priming arises at an earlier locus than the inhibition of categorial priming.

Resolving semantically induced tip-of-the-tongue states for proper nouns.

Three experiments are reported in which tip-of-the-tongue states (TOTSs) were induced in subjects by reading them pieces of item-specific information. In Experiments 1 and 2, subjects attempted to name famous people. These experiments showed that, in a TOTS, seeing a picture of the face of the target person did not facilitate naming, whereas the initials of the person's name did. In Experiment 3, a similar result was obtained with a landmark-naming task. The results of the experiments are discussed with reference to current models of memory structure and name retrieval.