Assuntos
Programas de Assistência Gerenciada/organização & administração , Motivação , Médicos/psicologia , Redução de Custos , Humanos , Satisfação no Emprego , Programas de Assistência Gerenciada/economia , Programas de Assistência Gerenciada/normas , Inovação Organizacional , Autonomia Profissional , Qualidade da Assistência à Saúde , Estados UnidosRESUMO
The Mo + PyF101 enhancer variant of Moloney murine leukemia virus (M-MuLV) has been very useful in investigating M-MuLV leukemogenesis. When inoculated subcutaneously (s.c.) into neonatal mice, Mo + PyF101 M-MuLV is attenuated for development of disease. Previous studies in mice infected with wild-type M-MuLV have revealed several important preleukemic events, including development of splenic hyperplasia, defects in bone marrow hematopoiesis, and in vivo generation of MCF viruses that arise by recombination in the uninfected mouse. Mo + PyF101 M-MuLV is defective in inducing these effects after s.c. inoculation. In the experiments reported here, a study of Mo + PyF101 M-MuLV infection in rats was carried out. Wild-type M-MuLV is leukemogenic in rats, but infected rats do not form MCF recombinants since they lack the necessary endogenous polytropic envelope sequences. Since Mo + PyF101 M-MuLV's leukemogenic defect is correlated with a failure to generate MCF recombinants, it seemed possible that wild-type M-MuLV might not have a leukemogenic advantage over Mo + PyF101 M-MuLV in rats, where MCF recombinants cannot form. Neonatal Fisher F344 rats were inoculated s.c. or intraperitoneally by wild-type and Mo + PyF101 M-MuLVs. Surprisingly, Mo + PyF101 M-MuLV was completely deficient in leukemogenesis in rats when inoculated by either route while wild-type M-MuLV induced lymphoma with the predicted time course. The leukemogenic defect for Mo + PyF101 M-MuLV resulted from a pronounced defect for establishing infection in rats. Further studies of wild-type M-MuLV in rats indicated that infection was confined almost exclusively to the thymus at early times. In mice wild-type M-MuLV establishes substantial infection in other hematopoietic organs such as spleen and bone marrow as well. Thymic infection was also correlated with a decrease in thymic cellularity at early times.
Assuntos
Elementos Facilitadores Genéticos , Variação Genética , Leucemia Experimental/virologia , Vírus da Leucemia Murina de Moloney/genética , Vírus da Leucemia Murina de Moloney/patogenicidade , Animais , Animais Recém-Nascidos , Leucemia Experimental/fisiopatologia , Camundongos , Camundongos Endogâmicos , Vírus da Leucemia Murina de Moloney/fisiologia , Ratos , Ratos Endogâmicos F344 , Infecções por Retroviridae/fisiopatologia , Infecções por Retroviridae/virologia , Especificidade da Espécie , Fatores de Tempo , Infecções Tumorais por Vírus/fisiopatologia , Infecções Tumorais por Vírus/virologiaAssuntos
Diretores de Hospitais/psicologia , Prestação Integrada de Cuidados de Saúde/organização & administração , Liderança , Tomada de Decisões Gerenciais , Eficiência Organizacional , Humanos , Equipes de Administração Institucional , Relações Interprofissionais , Satisfação no Emprego , Estudos de Casos Organizacionais , Desenvolvimento de Pessoal , Estados UnidosRESUMO
A recombinant retrovirus, termed MoFe2-MuLV, was constructed in which the U3 region of T-lymphomagenic Moloney murine leukemia virus (Mo-MuLV) was replaced by that of FeLV-945, a provirus of unique long terminal repeat (LTR) structure identified only in non-T-cell, non-B-cell lymphomas of the domestic cat. The LTR of FeLV-945 is unusual in that it contains only a single copy of the transcriptional enhancer followed 25 bp downstream by a 21-bp sequence in triplicate in tandem. Infectivity of MoFe2-MuLV was demonstrated in vitro in SC-1 cells and in vivo in neonatal NIH-Swiss mice. Tumors occurred in MoFe2-MuLV-infected animals following a latency period of 4 to 10 months (average, 6 months). The results of Southern blot analysis of the T-cell receptor beta locus demonstrated that all tumors were lymphomas of T-cell origin. MoFe2-MuLV LTRs were amplified by PCR from tumor DNA and were characterized by nucleotide sequence analysis. LTRs from the tumors that occurred with relatively shorter latency predominantly retained the original MoFe2-MuLV sequence intact and unaltered. Tumors that occurred with relatively longer latency contained LTRs that also retained the 21-bp sequence triplication characteristic of the original virus but had acquired various duplications of enhancer sequences. The repeated identification of enhancer duplications in late-appearing tumors suggests that the duplication affords a selective advantage, although apparently not in the efficient induction of T-cell lymphoma. Proto-oncogenes known to be targets of insertional mutagenesis in the majority of Mo-MuLV-induced tumors or in feline non-T-cell, non-B-cell lymphomas were shown not to be rearranged in any tumor examined. Mink cell focus-inducing (MCF) proviral DNA was readily detectable in some, but not all, tumors. The presence or absence of MCF did not correlate with the kinetics of tumor induction. These studies indicate that the single-enhancer, triplication-containing FeLV LTR, typical of non-T-cell, non-B-cell lymphomas in cats, is competent in the induction of T-cell lymphoma in mice. The findings suggest that the mechanism of MoFe2-MuLV-mediated lymphomagenesis may differ from that of Mo-MuLV-mediated disease, considering the possible involvement of novel oncogenes and the variable presence of MCF recombinants.
Assuntos
Vírus da Leucemia Felina/genética , Vírus da Leucemia Murina/genética , Leucemia Experimental/virologia , Vírus Reordenados/genética , Vírus Reordenados/patogenicidade , Retroviridae/genética , Retroviridae/patogenicidade , Animais , Gatos , Camundongos , Virulência/genéticaAssuntos
Leucemia Experimental/fisiopatologia , Vírus da Leucemia Murina de Moloney , Pré-Leucemia/fisiopatologia , Infecções por Retroviridae/fisiopatologia , Infecções Tumorais por Vírus/fisiopatologia , Animais , Medula Óssea/patologia , Hematopoese , Células-Tronco Hematopoéticas/patologia , Células-Tronco Hematopoéticas/fisiologia , Leucemia Experimental/patologia , Leucemia Experimental/virologia , Linfoma de Células T/patologia , Linfoma de Células T/fisiopatologia , Linfoma de Células T/virologia , Camundongos , Modelos Biológicos , Pré-Leucemia/patologia , Pré-Leucemia/virologia , Infecções por Retroviridae/patologia , Baço/patologia , Timo/patologia , Infecções Tumorais por Vírus/patologiaRESUMO
Prevention of high frequency spontaneous T cell lymphoma development in AKR mice by mAb 18-5 treatment was shown to involve inhibition of the recombinant Class I MCF virus formation and elimination of the early occurring potential lymphoma cells (PLCs). A low B cell lymphoma incidence (16% at a mean latency of 540 days) and a low level of PLCs (yielding 12% B cell lymphoma development following lymphoid cell transfer) was observed in mAb 18-5 treated mice (in contrast to a high PLC level in thymectomized AKR mice that could be experimentally triggered to progress to overt CD5+ B cell lymphomas). Administration of anti CD8 mAb or IL-4 to 12-month-old mAb 18-5 pre-treated mice only slightly increased B cell lymphoma incidence (up to 30-40%). Exposure to split-dose irradiation resulted in 26% B cell lymphomas at a 250 day mean latency. The phenotypes of the B lymphomas developing in mAb 18-5 treated mice were: B220+ (14.8+, 6B2+), 6C3+, Mac2+, CD5-. Most lymphomas expressed l-a and surface IgM, pointing to their mature B cell characteristics. Moreover, in some of the lymphomas, high levels of IgM production and secretion were determined. A comparison of the morphological characteristics (based on light and ultrastructure microscopy) of CD5+ and CD5- B cell lymphomas developing in AKR mice indicated marked differences. Analysis of the IgH locus of representative CD5- B lymphomas showed an identical pattern of IgH rearrangement in some tumors (similar to previous findings among CD5+ lymphomas). The virological analysis of the CD5- B cell lymphomas (similar to those observed in the CD5+ B cell lymphomas of AKR origin) showed that their development did not require formation of the pathogenic MCF recombinant viruses. The differences observed between the CD5+ and CD5- B cell lymphomas developing in AKR mice (following prevention of spontaneous T cell lymphomagenesis) may be due to their origin of different B cell precursors or from B cells at different levels of differentiation.
Assuntos
Linfoma de Células B/etiologia , Camundongos Endogâmicos AKR/imunologia , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Antígenos CD5/análise , DNA Viral/análise , Rearranjo Gênico de Cadeia Pesada de Linfócito B , Genes de Imunoglobulinas , Imunização Passiva , Imunoglobulinas/biossíntese , Imunofenotipagem , Linfoma de Células B/imunologia , Camundongos , Vírus Indutores de Focos em Células do Vison/imunologia , Infecções Tumorais por Vírus/prevenção & controleRESUMO
The AKR inbred mouse strain displays a high incidence of spontaneous T cell lymphomas that arise predominantly in the thymus of 6 to 12-month-old mice. Heterogenous nonacute transforming retroviruses are associated with the etiology of the disease: the endogenous ecotropic viruses (inherited in AKR mice at two non-linked chromosomal loci, Akv-1 and Akv-2), the xenotropic virus and recombinant viruses. Prevention of spontaneous T cell lymphomagenesis in AKR mice by passive anti-viral immunotherapy was accomplished by suppressing endogenous ecotropic virus release. Treatment with monoclonal antibody Hy-72 reacting only with Akv1 type ecotropic viruses, or with mAb 18-5 with specificity for both ecotropic and MCF recombinant virus envelope glycoprotein (administered from birth for 10 days) inhibited similarly T cell lymphoma development. A reduced thymus cellularity observed in these mAb treated mice coincided with reduced level of earliest intrathymic low CD4 precursor population in their thymus. The role of endogenous viruses (MuLV) and presence of potential lymphoma cells (PLCs) (identified among bone marrow cells of untreated AKR mice) in enhanced T cell lymphomagenesis in AKR mice, triggered by different leukemogenic agents, was evaluated. Intrathymic injection of the radiation leukemia virus variant A-RadLV or administration of methylnitrosourea resulted in a high lymphoma incidence within a short latent period of 80-100 days irrespective of the presence or absence of MuLV or PLCs in these treated mice. Thus, a direct action of these agents on thymocytes seems to occur. The high susceptibility of untreated AKR mice to radiation induced T cell lymphomagenesis was not affected by pretreatment with mAb Hy-72; in contrast to markedly reduced sensitivity following pretreatment with mAb 18-5 (15 vs 100%). The mAb 18-5 induced resistance to radiation lymphomagenesis seems to be related to defects in the bone marrow stem cell pool as well as in the thymus microenvironment of mAb 18-5 treated mice. Thus, different developmental pathways are involved in enhanced T cell lymphomagenesis in AKR mice.
Assuntos
Imunoterapia Adotiva , Linfoma de Células T/prevenção & controle , Animais , Anticorpos Monoclonais/uso terapêutico , Suscetibilidade a Doenças , Contagem de Linfócitos , Linfoma de Células T/induzido quimicamente , Linfoma de Células T/patologia , Linfoma de Células T/virologia , Metilnitrosoureia , Camundongos , Camundongos Endogâmicos AKR , Vírus da Leucemia Induzida por Radiação , Retroviridae/imunologia , Infecções por Retroviridae/patologia , Infecções por Retroviridae/prevenção & controle , Infecções por Retroviridae/virologia , Baço/patologia , Subpopulações de Linfócitos T/patologia , Timo/patologia , Infecções Tumorais por Vírus/patologia , Infecções Tumorais por Vírus/prevenção & controle , Infecções Tumorais por Vírus/virologiaRESUMO
We previously reported an in vitro T-cell differentiation system in which the L4 lymphoid clone was cocultured with the St3 stromal line derived from the same murine thymic tumor, 15#4T.L4 cells in L4-St3 cocultures sequentially express Thy-1 and CD4 in a manner typical of normal thymocytes. In contrast, L4 cells grown in medium alone retain their Thy-1-CD4- phenotype. We also isolated L4 subclones from the coculture with increasingly differentiated phenotypes with respect to Thy-1 and CD4. We now report induction of an additional thymocyte differentiation marker, terminal deoxynucleotidyl transferase (TdT) in 15#4T cells (and to a lesser extent subcloned L4 cells) upon coculture with St3 stroma. Coculture of 15#4T cells with St3 stroma resulted in expression of TdT as measured by ribonuclease protection for TdT RNA and Western immunoblotting for TdT protein. Cocultured L4 cells were induced for TdT expression to a lesser degree and for a shorter period of time. The magnitude of TdT RNA induction was maximal for cell lines with the least mature differentiation phenotype (15#4T and L4: Thy-1-CD4-) and decreased proportionally for subclones with increasingly mature phenotype, e.g., L4E cells (Thy-1+CD4+). TdT protein was undetectable by Western immunoblotting and immunofluorescent staining of the L4E subclone on or off stroma. Recombination-activating gene-1 (RAG-1), which is expressed in immature thymocytes during T-cell receptor rearrangement, but suppressed in mature thymocytes, was also examined using the ribonuclease protection assay.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Células do Tecido Conjuntivo , DNA Nucleotidilexotransferase/biossíntese , Proteínas de Ligação a DNA , Proteínas de Homeodomínio , Linfócitos T/enzimologia , Timo/citologia , Animais , Diferenciação Celular , Células Cultivadas , DNA Nucleotidilexotransferase/genética , Indução Enzimática , Regulação da Expressão Gênica , Camundongos , Biossíntese de Proteínas , Proteínas/genética , RNA Mensageiro/biossíntese , RNA Mensageiro/genéticaRESUMO
OBJECTIVE: Initial studies of protooncogenes in uterine corpus cancer have focused on a single aspect of the gene in question (deoxyribonucleic acid, ribonucleic acid, protein) or have studied a small number of patients. Therefore we evaluated c-myc and HER-2/neu gene amplification and ribonucleic acid overexpression in such malignancies and correlated these molecular changes with known pathologic risk factors. STUDY DESIGN: Quantitative Southern blot analysis for oncogene deoxyribonucleic acid was used to examine 37 tumors from patients with primary untreated uterine corpus cancer referred to the City of Hope National Medical Center. Six normal endometrial specimens were controls. Seventeen tumors were also examined by Northern blotting to assess increased ribonucleic expression. RESULTS: Histologic types included adenocarcinoma (n = 30), papillary serous adenocarcinoma (n = 2), adenosquamous carcinoma (n = 2), mixed mullerian sarcoma (n = 2), and leiomyosarcoma (n = 1). Carcinomas were stage I (n = 10), II (n = 18), or III (n = 6). Twenty-three had myometrial invasion of less than one third, six one third to two thirds, and eight deeper invasion (greater than two thirds). According to the criteria of the International Federation of Gynecology and Obstetrics stage was as follows: I (n = 22), II (n = 3), III (n = 7), and IV (n = 5). Ten (27%) and four (11%) tumors showed gene amplification of c-myc and HER-2/neu, respectively. Six demonstrated overexpression of either the c-myc or HER-2/neu gene. HER-2/neu gene amplification was associated more closely with overexpression. Stepwise logistic analysis demonstrated c-myc amplification to be associated with higher grade (p = 0.01). CONCLUSION: In this referral population, c-myc activation is more common than HER-2/neu activation in uterine corpus cancer and is associated with tumors of higher grade.
Assuntos
Adenocarcinoma/genética , Amplificação de Genes , Expressão Gênica , Genes erbB-2 , Genes myc , Neoplasias Uterinas/genética , Neoplasias Uterinas/patologia , Adenocarcinoma/patologia , Idoso , Autorradiografia , Northern Blotting , Southern Blotting , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
Mo+PyF101 M-MuLV is a variant Moloney murine leukemia virus containing polyomavirus F101 enhancers inserted just downstream from the M-MuLV enhancers in the long terminal repeat (LTR). The protein coding sequences for this virus are identical to those of M-MuLV. Mo+PyF101 M-MuLV induces T-cell disease with a much lower incidence and longer latency than wild-type M-MuLV. We have previously shown that Mo+PyF101 M-MuLV is defective in preleukemic events induced by wild-type M-MuLV, including splenic hematopoietic hyperplasia, bone marrow depletion, and generation of recombinant mink cell focus-inducing viruses (MCFs). We also showed that an M-MCF virus driven by the Mo+PyF101 LTR is infectious in vitro but does not propagate in mice. However, in these experiments, when a pseudotypic mixture of Mo+PyF101 M-MuLV and Mo+PyF101 MCF was inoculated into newborn NIH Swiss mice, they died of T-cell leukemia at times almost equivalent to those induced by wild-type M-MuLV. Tumor DNAs from Mo+PyF101 M-MuLV-Mo+PyF101 MCF-inoculated mice were examined by Southern blot analysis. The predominant forms of Mo+PyF101 MCF proviruses in these tumors contained added sequences in the U3 region of the LTR. The U3 regions of representative tumor-derived variant Mo+PyF101 MCFs were cloned by polymerase chain reaction amplification, and sequencing indicated that they had acquired an additional copy of the M-MuLV 75-bp tandem repeat in the enhancer region. NIH 3T3 cell lines infected with altered viruses were obtained from representative Mo+PyF101 M-MuLV-Mo+PyF101 MCF-induced tumors, and mice were inoculated with the recovered viruses. Leukemogenicity was approximately equivalent to that in the original Mo+PyF101 M-MuLV-Mo+PyF101 MCF viral stock. Southern blot analysis on the resulting tumors now predominantly revealed loss of the polyomavirus sequences. These results suggest that the suppressive effects of the PyF101 sequences on M-MuLV-induced disease and potentially on MCF propagation were overcome in two ways: by triplication of the M-MuLV direct repeats and by loss of the polyomavirus sequences.
Assuntos
Leucemia Experimental/genética , Vírus Indutores de Focos em Células do Vison/genética , Vírus da Leucemia Murina de Moloney/genética , Sequências Repetitivas de Ácido Nucleico/genética , Infecções por Retroviridae/genética , Infecções Tumorais por Vírus/genética , Células 3T3 , Animais , Sequência de Bases , Clonagem Molecular , DNA de Neoplasias/genética , Variação Genética , Leucemia Experimental/etiologia , Leucemia Experimental/microbiologia , Leucemia de Células T/etiologia , Leucemia de Células T/genética , Leucemia de Células T/microbiologia , Camundongos , Camundongos Endogâmicos , Dados de Sequência Molecular , Vírus da Leucemia Murina de Moloney/patogenicidade , Provírus/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , VirulênciaRESUMO
We have used the L4E murine thymoma-derived T-lymphoid cell line to study mechanisms involved in CD4 expression. When L4E cells are cocultured with the St3 stromal cell line, surface CD4 expression is maintained, even across a membrane. However, when transferred to medium alone, these cells rapidly lose CD4. We have tested whether CD4 can be maintained on CD4+ L4E cells when they are transferred to medium containing known cytokines. In these results, rmTNF-alpha maintained surface CD4 expression at significant levels on L4E cells. In addition, rmTNF-alpha could induce CD4 on CD4- L4E cells (obtained by growth in medium alone). Despite these results CD4 induction and maintenance in L4E cells by coculture with St3 stroma did not appear to result from secretion of TNF-alpha, since St3 cells did not express TNF-alpha mRNA, secreted TNF-alpha was not detected by ELISA assay in supernatant from St3 cells nor L4E-St3 cocultures, and incubation with neutralizing anti-TNF-alpha antibody did not inhibit CD4 maintenance. The ability of TNF-alpha to affect CD4 expression on L4E cells supports a role in thymocyte differentiation for this cytokine.
Assuntos
Antígenos CD4/biossíntese , Linfócitos T/efeitos dos fármacos , Fator de Necrose Tumoral alfa/farmacologia , Animais , Linhagem Celular , Meios de Cultivo Condicionados , Citocinas/farmacologia , Citotoxicidade Imunológica/efeitos dos fármacos , Citometria de Fluxo , Humanos , Camundongos , Mitógenos/farmacologia , Proteínas Recombinantes/farmacologia , Linfócitos T/imunologia , Timoma , Neoplasias do Timo , Células Tumorais CultivadasRESUMO
In the AKR.Fv-1b congenic strain the Fv-1n allele of the AKR/J mice was substituted with the Fv-1b allele, thereby limiting viral replication and spread of the endogenous N-tropic murine leukemia virus. As a result of this genetic change AKR.Fv-1b mice develop a low spontaneous incidence (7%) of T-cell lymphomas and about 28% of Ly-1+ B-cell lymphomas are observed in old mice. Characteristic changes in thymus subpopulations of AKR/J mice (related to the formation of the dual tropic mink cell focus inducing (MCF) type virus in the thymus) were not observed in the thymus of AKR.Fv-1b mice. In contrast to the low susceptibility to spontaneous T-cell lymphoma development, these mice were highly sensitive to fractionated irradiation or to radiation leukemia virus (a mixture of N- and B-tropic viruses) induced T-cell lymphoma. Potential lymphoma cells (that would ultimately develop into Ly-1+ B-cell lymphomas) were demonstrated in bone marrow and spleens of 16-24-month-old mice. Analysis of the Ly-1+ IgM+ B-cell population in spleens of 18-month-old mice revealed a significant increase in this population (35% versus 2% in young spleens). The spontaneous Ly-1+ B-cell lymphoma incidence could be enhanced (up to 77%) by in vivo administration of anti-CD8 monoclonal antibody or IL-4 to 18-month-old mice. Virological analysis of T/B-cell lymphomas for class I MCF viruses indicated that Class I MCF development was tightly correlated with T-lymphoma development (except radiation induced tumors that showed no MCF provirus involvement). In contrast, Ly-1+ B-cell lymphoma development was independent of Class I MCF pathogenic virus involvement.
Assuntos
Linfoma de Células B/etiologia , Linfoma de Células T/etiologia , Camundongos Endogâmicos AKR , Neoplasias do Timo/etiologia , Vírus AKR da Leucemia Murina/genética , Vírus AKR da Leucemia Murina/imunologia , Animais , Antígenos de Diferenciação/análise , Feminino , Genes Virais , Linfoma de Células B/imunologia , Linfoma de Células B/microbiologia , Linfoma de Células T/imunologia , Linfoma de Células T/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos AKR/genética , Neoplasias do Timo/imunologia , Neoplasias do Timo/microbiologiaRESUMO
Primary lymphoid tumor cells induced by wild type or recombinant Moloney murine leukemia viruses were inoculated intravenously into sublethally irradiated, syngeneic mice and compared for their ability to reestablish in the recipient thymus and spleen. Lymphoid tumors induced by one recombinant, M-MuLV(myc), containing the v-myc oncogene from the avian MC29 retrovirus consistently reestablished in the thymus and spleen of recipient mice. Since tumors in the recipient thymus and spleen could have arisen by infection of host cells, or could reflect an expansion of a minor subpopulation from the donor tumor, we verified the donor and clonal origin of these tumors by Southern blot analysis using a virus-specific probe. As few as 500 of these tumor cells could migrate to the thymus via the blood and flourish in that microenvironment. In contrast, the majority of tumors induced by wild type M-MuLV or two other M-MuLV recombinants not containing v-myc sequences, inefficiently reestablished in the thymus of recipient mice following i.v. inoculation, although splenic tumors developed. These tumors could, however, multiply within the recipient thymus when inoculated intrathymically. Thus, the inability of tumors induced by wild type M-MuLV or M-MuLV recombinants lacking v-myc to reestablish in the thymus following i.v. inoculation appears to reflect inefficient 'thymic homing' rather than the ability to grow in the thymic microenvironment. Collectively, our data suggest that the thymic homing ability of M-MuLV(myc)-induced tumor cells is related to the presence of v-myc sequences or to the cell type transformed by M-MuLV(myc). Thus, we suggest that these cells may provide a useful model for studying how blood-borne metastatic cells, and possibly normal lymphocytes, enter the thymus by way of the microvasculature.
Assuntos
Genes myc , Vírus da Leucemia Murina/genética , Leucemia Experimental/etiologia , Neoplasias do Timo/etiologia , Animais , Divisão Celular , Linfócitos/fisiologia , CamundongosRESUMO
AKR mice are highly susceptible to spontaneous T cell lymphomagenesis and thymus removal at the age of 1 to 3 mo greatly reduces its development. Twelve-mo-old AKR mice thymectomized at young age were shown previously to carry potential lymphoma cells that could be triggered to develop into B cell lymphomas (80 to 100%) after removal from their host "restrictive" environment into young histocompatible hosts. Additional attempts were made to terminate the potential lymphoma cell dormant state in 12-mo-old thymectomized AKR mice. Replenishment of some deficiencies caused by thymectomy at a young age, including a s.c. syngeneic thymus graft or a single injection of the dual tropic recombinant virus isolates DTV-71 or MCF-247 into 12-mo-old thymectomized AKR mice resulted in Ly-1+ pre-B or B cell lymphoma development in 80 to 98% of these treated mice. In vivo elimination of T cell subsets by administration of cyclosporin A or by mAb expressed on Th cells (anti-CD4) or cytotoxic T cells (anti-CD8) stimulated the progression of dormant potential lymphoma cells towards B cell lymphoma development. The most striking results were observed after administration of anti-CD8 mAb: 90 to 100% of these treated mice developed Ly-1+ B cell lymphomas within 80 days. The effect of rIL-2 on dormant PLC was also tested. Administration of rIL-2 to 12-mo-old thymectomized mice terminated tumor dormancy in 94% of the treated mice within 66 days. Tests of the resulting B lymphomas for dual tropic recombinant virus/mink cell focus-inducing virus infection indicated that the breakdown of tumor dormancy did not result from development of pathogenic class I mink cell focus-inducing viruses. These results suggest that T cell subsets and/or their products are involved in the proliferation arrest of potential lymphoma cells present in thymectomized AKR mice.
Assuntos
Linfoma de Células B/etiologia , Timo/imunologia , Animais , Southern Blotting , Antígenos CD8/fisiologia , Interleucina-2/farmacologia , Linfoma de Células B/genética , Linfoma de Células B/microbiologia , Camundongos , Camundongos Endogâmicos AKR , Vírus Indutores de Focos em Células do Vison/patogenicidade , Polimorfismo de Fragmento de Restrição , Proteínas Recombinantes/farmacologia , Mapeamento por Restrição , Subpopulações de Linfócitos T/imunologia , TimectomiaRESUMO
Neonatal CxD2 (Rmcfr) and Balb/c (Rmcfs) mice inoculated with Moloney murine leukemia virus (M-MuLV) exhibited approximately equivalent time course and pathology for disease. CxD2 mice showed only slightly reduced presence of Moloney mink cell focus-forming virus (M-MCF) provirus as seen by Southern blot analysis compared to Balb/c mice. This lack of restriction for disease and spread of MCF was in sharp contrast to that seen for CxD2 mice inoculated with Friend murine leukemia virus (F-MuLV), where incidence of disease and propagation of MCFs were severely restricted, as previously reported. Inoculation of CxD2 mice with FM-MuLV, a recombinant F-MuLV virus containing M-MuLV LTR sequences (U3 and R), resulted in T cell disease of time course equal to that seen in Balb/c mice; there also was little restriction for propagation of MCFs. This indicated that presence of the M-MuLV long terminal repeat (LTR) was sufficient for propagation of MCFs in CxD2 mice. Differing restriction for F-MuLV vs. M-MuLV in CxD2 mice was explained on the basis of different "MCF propagator cells" for the two viruses. It was suggested that cells propagating F-MCF (e.g., erythroid progenitors) are blocked by endogenous MCF-like gp70env protein, whereas cells propagating M-MCF (e.g., lymphoid) do not express this protein on their surface. F-MuLV disease in CxD2 mice was greatly accelerated when neonates were inoculated with a F-MuLV/F-MCF pseudotypic mixture. However, F-MCF provirus was not detectable or only barely detectable in F-MuLV/F-MCF-induced tumors, suggesting that F-MCF acted indirectly in induction of these tumors.
Assuntos
DNA Viral , Vírus da Leucemia Murina de Friend , Leucemia Experimental/imunologia , Vírus da Leucemia Murina de Moloney , Sequências Repetitivas de Ácido Nucleico , Alelos , Animais , Southern Blotting , Clonagem Molecular , DNA de Neoplasias/análise , Imunidade Inata/genética , Leucemia Experimental/genética , Leucemia Experimental/mortalidade , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos DBA , Proteínas Oncogênicas de Retroviridae/genética , Linfócitos T/imunologia , Proteínas do Envelope Viral/genéticaRESUMO
Moloney murine leukemia virus (Mo-MuLV) induces T-cell lymphoma when inoculated into neonatal mice. This is a multistep process. Early events observed in infected mice include generalized hematopoietic hyperplasia in the spleen and appearance of mink cell focus-inducing (MCF) recombinants; end-stage tumors are characterized by insertional proviral activation of protooncogenes. We previously showed that an Mo-MuLV enhancer variant, Mo+PyF101 Mo-MuLV, has greatly reduced leukemogenicity and is deficient in induction of preleukemic hyperplasia. In this report, we have examined Mo+PyF101 Mo-MuLV-inoculated mice for the presence of MCF recombinants. In contrast to wild-type Mo-MuLV-inoculated mice, Mo+PyF101 Mo-MuLV-inoculated mice did not generate detectable MCF recombinants. This failure was at least partly due to an inability of the MCF virus to propagate in vivo, since a molecularly cloned infectious Mo+PyF101 MCF virus did not replicate, even when inoculated as a Mo+PyF101 Mo-MuLV pseudotype. These results show that the leukemogenic defect of Mo+PyF101 Mo-MuLV is associated with its inability to generate MCF recombinants capable of replication in vivo. This, in turn, is consistent with the view that MCF recombinants play a significant role in Mo-MuLV-induced disease and, in particular, may play a role early in the disease process.
Assuntos
Transformação Celular Neoplásica , Vírus Defeituosos/genética , Elementos Facilitadores Genéticos , Variação Genética , Leucemia Experimental/microbiologia , Vírus Indutores de Focos em Células do Vison/genética , Vírus da Leucemia Murina de Moloney/genética , Pré-Leucemia/microbiologia , Animais , Linhagem Celular , Células L , Camundongos , Vírus da Leucemia Murina de Moloney/patogenicidade , Provírus/genéticaRESUMO
We previously showed that neonatal mice inoculated with Moloney murine leukemia virus (M-MuLV) exhibit a preleukemic state characterized by splenomegaly and increased numbers of hematopoietic progenitors. An M-MuLV variant with greatly reduced leukemogenic potential, Mo+PyF101 M-MuLV, does not generally induce this preleukemic state. In order to investigate the mechanism involved in M-MuLV induction of preleukemic hyperplasia, we tested the CFU-mixed myeloid and erythroid (CFUmix) from M-MuLV- and Mo+PyF101 M-MuLV-inoculated mice for the presence of virus by antibody staining and for the release of infectious virus. The majority of CFUmix colonies from both M-MuLV- and Mo+PyF101 M-MuLV-inoculated mice contained infectious virus even though M-MuLV-inoculated mice showed elevated levels of CFUmix while the Mo+PyF101 M-MuLV-inoculated mice did not. This indicates that direct infection of hematopoietic progenitors was not sufficient to induce hyperplasia. Rather, hematopoietic hyperplasia may result indirectly from infection of some other cell type.
Assuntos
Células-Tronco Hematopoéticas/patologia , Leucemia Experimental/microbiologia , Vírus da Leucemia Murina de Moloney/patogenicidade , Pré-Leucemia/microbiologia , Animais , Ensaio de Unidades Formadoras de Colônias , Produtos do Gene gag/análise , Hiperplasia , Técnicas Imunoenzimáticas , Leucemia Experimental/patologia , Camundongos , Camundongos Endogâmicos , Pré-Leucemia/patologia , Baço/microbiologia , Baço/patologiaRESUMO
Subjects scoring above and below a sample median on the Beck Depression Inventory were randomly assigned to one of four experimental conditions in which they were exposed to either (1) an unsolvable task accompanied by unavoidable aversive noise, (2) an identical but solvable task whose solution prevented the noise, or conditions designed to control for the effects of (3) the aversiveness of the noise, or (4) possible baseline performance differences between subject groups. The adolescents in the depressed group who were exposed to the unsolvable task showed a significant performance deficit on a subsequent solvable task when compared to their counterparts in the other three conditions. Subjects in the nondepressed group showed no such deficit, suggesting that as level of depressive symptomatology increases, adolescents become more vulnerable to suffering a disruption of active coping (effort, persistence, problem solving) when confronted with uncontrollable events. Theoretical and treatment issues related to adolescent depression are discussed.
RESUMO
We have cloned both T lymphoid and stromal lines from a single murine thymic tumor that was induced by a retrovirus carrying the v-myc oncogene (M-MuLV(myc]. The T lymphoid line, L4, was cloned by growth in agar. L4 cells were initially negative for Thy-1.2 and CD4 (although they contained rearranged TCR-beta genes), and they remained so if passaged in medium alone. However, cocultivation of these Thy-1.2- CD4- cells with the cloned stromal cell line, St3, resulted in sequential expression of Thy-1.2 and CD4 in subpopulations of cells. Thy-1.2+ CD4- and Thy-1.2+ CD4+ L4 subclones were obtained from the cocultures by subsequent cloning in agar. Derivation of these subclones from the starting Thy-1.2- CD4- clone was verified by Southern blot analyses specific for TCR-beta gene rearrangements and for M-MuLV(myc) proviral integration sites. Continuous cocultivation of Thy-1.2+ CD4+ L4 subclones with the St3 stromal cells was necessary for maintenance of CD4 on the cell surface. Furthermore, CD4 expression which was lost when CD4+ L4 cells were removed from the stroma could be reinduced if they were again cultured on St3 stroma. These cells may provide a model system for studying thymocyte-stromal cell interactions in induction and maintenance of expression of Thy-1 and CD4 molecules.
Assuntos
Antígenos de Superfície/imunologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T/citologia , Timo/imunologia , Animais , Antígenos de Diferenciação de Linfócitos T/imunologia , Northern Blotting , Linfócitos T CD4-Positivos/citologia , Comunicação Celular , Diferenciação Celular , Células Cultivadas , Citometria de Fluxo , Regulação da Expressão Gênica , Rearranjo Gênico da Cadeia beta dos Receptores de Antígenos dos Linfócitos T , Técnicas In Vitro , Camundongos , Receptores de Antígenos de Linfócitos T/genética , Linfócitos T/imunologia , Antígenos Thy-1 , Timo/citologiaRESUMO
Lymphoid tumors induced by a recombinant murine retrovirus carrying the v-myc oncogene of avian MC29 virus were characterized. The Moloney murine leukemia virus myc oncogene (M-MuLV (myc], carried by an amphotropic MuLV helper, induced tumors in NIH Swiss and NFS/N mice after a relatively long latency (8 to 24 wk). Tumor masses appeared in the thymus, spleen, and lymph nodes. Flow cytometry of the tumor cells indicated that approximately 50% were positive for Thy 1.2. Most of these tumors also expressed one or more other cell surface markers of thymocytes and mature T cells (CD4, CD8). Southern blot hybridization revealed genomic rearrangements for the TCR beta genes. The TCR beta analysis suggested that the M-MuLV(myc)-induced Thy 1.2+ tumors were derived from somewhat less mature cells than tumors induced by M-MuLV, which is a classical non-acute retrovirus lacking an oncogene. The remainder of the M-MuLV(myc)-induced tumors were Thy 1.2-, but they were positive for Ly-5 (B220) and also for MAC-2. The Thy 1.2- tumors were characteristically located in the thymus. However, they were negative for TCR beta gene rearrangements. Some, but not all, of the Thy 1.2- tumors contained rearrangements for Ig genes. Additionally, they typically expressed mRNA specific for B but not for T cells. Thus, these thymic tumors had characteristics of the B cell lineage. Tumor transplantation experiments demonstrated that the Thy 1.2- tumor cells could reestablish in the thymus and spleen of irradiated hosts, and low level expression of the Thy 1 molecule was observed in the thymus but not the spleen on the first passage. After serial passage, one Thy 1- tumor altered its cell surface phenotype to Thy 1low B220-.