RESUMO
There is currently no clear understanding on the pathways involved in the process of cell inhibition by photobiomodulation (PBM). The present study evaluated the influence of PBM on the expression of autophagy markers in vitro in an in situ model of oral carcinoma. Oral squamous cell carcinoma (Cal27) and stromal fibroblasts (FG) cultures were used. The independent variables were 'cell type' (FG and CAL27) 'culture condition' (monocultures or co-cultures) and PBM (placebo and 36 J/cm2). The cultures were irradiated from a red LED source for mRNA expression and protein expression analyses. The autophagy markers evaluated were Beclin-1, LC3B and p62 as well as adjuvant markers (BAX Bcl-2, VEGF, CD105, CD34, PRDX1, PRDX4 and GRP78). The Cal27 cells upregulated the autophagy markers upon exposure to PBM both at the mRNA and protein expression levels, providing evidence to explain malignant cell inhibition by PBM.
Assuntos
Autofagia/genética , Luz , Regulação para Cima/efeitos da radiação , Proteína Beclina-1/genética , Proteína Beclina-1/metabolismo , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Linhagem Celular , Técnicas de Cocultura , Chaperona BiP do Retículo Endoplasmático , Humanos , Proteínas Associadas aos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/metabolismo , Neoplasias Bucais/metabolismo , Neoplasias Bucais/patologia , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , RNA Mensageiro/metabolismoAssuntos
Adenoma/metabolismo , Biomarcadores Tumorais/metabolismo , Carcinoma/metabolismo , Proteínas de Ligação a DNA/metabolismo , Neoplasias das Glândulas Salivares/metabolismo , Fatores de Transcrição/metabolismo , Adenoma/diagnóstico , Adenoma/patologia , Carcinoma/diagnóstico , Carcinoma/patologia , Proteínas de Ligação a DNA/genética , Diagnóstico Diferencial , Humanos , Imuno-Histoquímica , Metástase Neoplásica , Neoplasias das Glândulas Salivares/diagnóstico , Neoplasias das Glândulas Salivares/patologia , Glândulas Salivares/metabolismo , Glândulas Salivares/patologia , Glândula Tireoide/metabolismo , Glândula Tireoide/patologia , Fatores de Transcrição/genéticaRESUMO
Gingival cyst of the adult is a rare slow growing and asymptomatic lesion that arises from the rests of the dental lamina. The present report describes the case of a miniscule adult gingival cyst in the lower anterior gingiva in a 51-year-old male with agenesis of lower premolars and lateral incisors. This paper contrasts the literature concerning the differentiation between the gingival cyst of the adult and the lateral periodontal cysts as well as the possible misguided concept that the former may be such rare an occurrence.