CC-seq: Nucleotide-Resolution Mapping of Spo11 DNA Double-Strand Breaks in S. cerevisiae Cells.

During meiosis, Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5' ends on both sides of the break during this process. Such Spo11 "covalent complexes" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronized Saccharomyces cerevisiae meiotic cells.

Single-cell measurement of microbial growth rate with Raman microspectroscopy.

Rates of microbial growth are fundamental to understanding environmental geochemistry and ecology. However, measuring the heterogeneity of microbial activity at the single-cell level, especially within complex populations and environmental matrices, remains a forefront challenge. Stable isotope probing (SIP) is a method for assessing microbial growth and involves measuring the incorporation of an isotopic label into microbial biomass. Here, we assess Raman microspectroscopy as a SIP technique, specifically focusing on the measurement of deuterium (2H), a tracer of microbial biomass production. We correlatively measured cells grown in varying concentrations of deuterated water with both Raman spectroscopy and nanoscale secondary ion mass spectrometry (nanoSIMS), generating isotopic calibrations of microbial 2H. Relative to Raman, we find that nanoSIMS measurements of 2H are subject to substantial dilution due to rapid exchange of H during sample washing. We apply our Raman-derived calibration to a numerical model of microbial growth, explicitly parameterizing the factors controlling growth rate quantification and demonstrating that Raman-SIP can sensitively measure the growth of microorganisms with doubling times ranging from hours to years. The measurement of single-cell growth with Raman spectroscopy, a rapid, nondestructive technique, represents an important step toward application of single-cell analysis into complex sample matrices or cellular assemblages.

Meiotic prophase length modulates Tel1-dependent DNA double-strand break interference.

During meiosis, genetic recombination is initiated by the formation of many DNA double-strand breaks (DSBs) catalysed by the evolutionarily conserved topoisomerase-like enzyme, Spo11, in preferred genomic sites known as hotspots. DSB formation activates the Tel1/ATM DNA damage responsive (DDR) kinase, locally inhibiting Spo11 activity in adjacent hotspots via a process known as DSB interference. Intriguingly, in S. cerevisiae, over short genomic distances (<15 kb), Spo11 activity displays characteristics of concerted activity or clustering, wherein the frequency of DSB formation in adjacent hotspots is greater than expected by chance. We have proposed that clustering is caused by a limited number of sub-chromosomal domains becoming primed for DSB formation. Here, we provide evidence that DSB clustering is abolished when meiotic prophase timing is extended via deletion of the NDT80 transcription factor. We propose that extension of meiotic prophase enables most cells, and therefore most chromosomal domains within them, to reach an equilibrium state of similar Spo11-DSB potential, reducing the impact that priming has on estimates of coincident DSB formation. Consistent with this view, when Tel1 is absent but Ndt80 is present and thus cells are able to rapidly exit meiotic prophase, genome-wide maps of Spo11-DSB formation are skewed towards pericentromeric regions and regions that load pro-DSB factors early-revealing regions of preferential priming-but this effect is abolished when NDT80 is deleted. Our work highlights how the stochastic nature of Spo11-DSB formation in individual cells within the limited temporal window of meiotic prophase can cause localised DSB clustering-a phenomenon that is exacerbated in tel1Δ cells due to the dual roles that Tel1 has in DSB interference and meiotic prophase checkpoint control.

Chromatin binding by HORMAD proteins regulates meiotic recombination initiation.

The meiotic chromosome axis coordinates chromosome organization and interhomolog recombination in meiotic prophase and is essential for fertility. In S. cerevisiae, the HORMAD protein Hop1 mediates the enrichment of axis proteins at nucleosome-rich islands through a central chromatin-binding region (CBR). Here, we use cryoelectron microscopy to show that the Hop1 CBR directly recognizes bent nucleosomal DNA through a composite interface in its PHD and winged helix-turn-helix domains. Targeted disruption of the Hop1 CBR-nucleosome interface causes a localized reduction of axis protein binding and meiotic DNA double-strand breaks (DSBs) in axis islands and leads to defects in chromosome synapsis. Synthetic effects with mutants of the Hop1 regulator Pch2 suggest that nucleosome binding delays a conformational switch in Hop1 from a DSB-promoting, Pch2-inaccessible state to a DSB-inactive, Pch2-accessible state to regulate the extent of meiotic DSB formation. Phylogenetic analyses of meiotic HORMADs reveal an ancient origin of the CBR, suggesting that the mechanisms we uncover are broadly conserved.

Soil macrofauna communities in Brazilian land-use systems.

Background: Soil animal communities include more than 40 higher-order taxa, representing over 23% of all described species. These animals have a wide range of feeding sources and contribute to several important soil functions and ecosystem services. Although many studies have assessed macroinvertebrate communities in Brazil, few of them have been published in journals and even fewer have made the data openly available for consultation and further use. As part of ongoing efforts to synthesise the global soil macrofauna communities and to increase the amount of openly-accessible data in GBIF and other repositories related to soil biodiversity, the present paper provides links to 29 soil macroinvertebrate datasets covering 42 soil fauna taxa, collected in various land-use systems in Brazil. A total of 83,085 georeferenced occurrences of these taxa are presented, based on quantitative estimates performed using a standardised sampling method commonly adopted worldwide to collect soil macrofauna populations, i.e. the TSBF (Tropical Soil Biology and Fertility Programme) protocol. This consists of digging soil monoliths of 25 x 25 cm area, with handsorting of the macroinvertebrates visible to the naked eye from the surface litter and from within the soil, typically in the upper 0-20 cm layer (but sometimes shallower, i.e. top 0-10 cm or deeper to 0-40 cm, depending on the site). The land-use systems included anthropogenic sites managed with agricultural systems (e.g. pastures, annual and perennial crops, agroforestry), as well as planted forests and native vegetation located mostly in the southern Brazilian State of Paraná (96 sites), with a few additional sites in the neighbouring states of São Paulo (21 sites) and Santa Catarina (five sites). Important metadata on soil properties, particularly soil chemical parameters (mainly pH, C, P, Ca, K, Mg, Al contents, exchangeable acidity, Cation Exchange Capacity, Base Saturation and, infrequently, total N), particle size distribution (mainly % sand, silt and clay) and, infrequently, soil moisture and bulk density, as well as on human management practices (land use and vegetation cover) are provided. These data will be particularly useful for those interested in estimating land-use change impacts on soil biodiversity and its implications for below-ground foodwebs, ecosystem functioning and ecosystem service delivery. New information: Quantitative estimates are provided for 42 soil animal taxa, for two biodiversity hotspots: the Brazilian Atlantic Forest and Cerrado biomes. Data are provided at the individual monolith level, representing sampling events ranging from February 2001 up to September 2016 in 122 sampling sites and over 1800 samples, for a total of 83,085 ocurrences.

Enhancing health outcomes for Maori elders through an intergenerational cultural exchange and physical activity programme: a cross-sectional baseline study.

Background: The study offers baseline data for a strengths-based approach emphasizing intergenerational cultural knowledge exchange and physical activity developed through a partnership with kaumatua (Maori elders) and kaumatua service providers. The study aims to identify the baseline characteristics, along with correlates of five key outcomes. Methods: The study design is a cross-sectional survey. A total of 75 kaumatua from six providers completed two physical functioning tests and a survey that included dependent variables based in a holistic model of health: health-related quality of life (HRQOL), self-rated health, spirituality, life satisfaction, and loneliness. Results: The findings indicate that there was good reliability and moderate scores on most variables. Specific correlates included the following: (a) HRQOL: emotional support (ß = 0.31), and frequent interaction with a co-participant (ß = 0.25); (b) self-rated health: frequency of moderate exercise (ß = 0.32) and sense of purpose (ß = 0.27); (c) spirituality: sense of purpose (ß = 0.46), not needing additional help with daily tasks (ß = 0.28), and level of confidence with cultural practices (ß = 0.20); (d) life satisfaction: sense of purpose (ß = 0.57), frequency of interaction with a co-participant (ß = -0.30), emotional support (ß = 0.25), and quality of relationship with a co-participant (ß = 0.16); and (e) lower loneliness: emotional support (ß = 0.27), enjoyment interacting with a co-participant (ß = 0.25), sense of purpose (ß = 0.24), not needing additional help with daily tasks (ß = 0.28), and frequency of moderate exercise (ß = 0.18). Conclusion: This study provides the baseline scores and correlates of important social and health outcomes for the He Huarahi Tautoko (Avenue of Support) programme, a strengths-based approach for enhancing cultural connection and physical activity.

The use of ICSI in ART: evidence for practice.

This article reviews the evidence regarding the safety and efficacy of intra-cytoplasmic sperm injection (ICSI). It provides evidence-based clinical and laboratory guidelines and recommendations for use of ICSI within an assisted reproductive technology (ART) service. The guidelines address the evidence for the use of ICSI rather than conventional IVF (cIVF); the use of ART techniques supplementary to ICSI; and risks associated with ICSI. This article is not intended to be the only approved standard of practice or to dictate an exclusive course of treatment. Other plans of management may be appropriate, taking into account the needs and medical history of the patient, available resources, and institutional or clinical practice limitations.

Amazonian earthworm biodiversity is heavily impacted by ancient and recent human disturbance.

Despite the importance of earthworms for soil formation, more is needed to know about how Pre-Columbian modifications to soils and the landscape. Gaining a deeper understanding is essential for comprehending the historical drivers of earthworm communities and the development of effective conservation strategies in the Amazon rainforest. Human disturbance can significantly impact earthworm diversity, especially in rainforest soils, and in the particular case of the Amazonian rainforest, both recent and ancient anthropic practices may be important. Amazonian Dark Earths (ADEs) are fertile soils found throughout the Amazon Basin, created by sedentary habits and intensification patterns of pre-Colombian societies primarily developed in the second part of the Holocene period. We have sampled earthworm communities in three Brazilian Amazonian (ADEs) and adjacent reference soils (REF) under old and young forests and monocultures. To better assess taxonomic richness, we used morphology and the barcode region of the COI gene to identify juveniles and cocoons and delimit Molecular Operational Taxonomic Units (MOTUs). Here we suggest using Integrated Operational Taxonomical units (IOTUs) which combine both morphological and molecular data and provide a more comprehensive assessment of diversity, while MOTUs only rely on molecular data. A total of 970 individuals were collected, resulting in 51 taxonomic units (IOTUs, MOTUs, and morphospecies combined). From this total, 24 taxonomic units were unique to REF soils, 17 to ADEs, and ten were shared between both soils. The highest richness was found in old forest sites for ADEs (12 taxonomic units) and REFs (21 taxonomic units). The beta-diversity calculations reveal a high species turnover between ADEs and REF soils, providing evidence that ADEs and REFs possess distinct soil biota. Furthermore, results suggest that ADE sites, formed by Pre-Columbian human activities, conserve a high number of native species in the landscape and maintain a high abundance, despite their long-term nature.

Selection and Outcomes for Dissolution Therapy in Uric Acid Stones: A Systematic Review of Literature.

PURPOSE OF REVIEW: The prevalence of uric acid (UA) urolithiasis contributes significantly to global disease burden, due to high rates of recurrence and diagnostic challenges. Dissolution therapy plays a valuable role in the conservative management of UA calculi, reducing the requirement for surgical intervention. This review summarises the existing evidence for the efficacy of medical dissolution of uric acid urolithiasis. RECENT FINDINGS: A systematic search was conducted of worldwide literature according to PRISMA methodology and Cochrane standards for systematic review. Studies were included if they reported outcome data for the administration of medical therapy for the dissolution of UA calculi. A total of 1075 patients were included in the systematic review. Complete or partial dissolution of UA calculi was observed in 80.5% of patients (865/1075 patients), with 61.7% (647/1048 patients) achieving complete dissolution and 19.8% (207/1048 patients) achieving partial dissolution. A discontinuation rate of 10.2% (110/1075 patients) was noted, and 15.7% (169/1075 patients) required surgical intervention. Dissolution therapy is a safe and effective method of conservatively managing uric acid stones in the short term. Despite the significant disease burden of UA calculi, current guidelines are limited by deficiencies in the existing body of research. Further research should be undertaken to develop evidence-based clinical guidelines for diagnosis, treatment, and prevention of UA urolithiasis.

Earthworms (Clitellata, Megadrili) of the world: an updated checklist of valid species and families, with notes on their distribution.

In the current paper we present an updated checklist of all the megadrile earthworms (Crassiclitellata: Annelida) in the world, and notes on the distribution of families worldwide. Biogeographic responses to geological phenomena including plate tectonics, as well as to past and present climate and habitat distributions, are the main factors determining the present distribution of earthworm families. A total of ca. 5,738 species/subspecies (5,406 species and 332 unique subspecies; i.e., not counting the nomino-typical subspecies) belonging to 23 families (including one non-crassiclitellate family: Moniligastridae) are currently recognized worldwide, of which three families (Tritogeniidae and Kazimierzidae from Southern Africa and Arecoidae, a new family from Brazil described herein), 35 genera and close to 1200 new taxa (including subspecies) were described in the 21st century. Nonetheless, the large number of still undescribed species will likely increase this value to well over 8,000 species. Ten families are monospecific and/or monogeneric and have a mostly restricted distribution. On the other hand, more than 87 widespread cosmopolitan species have been catalogued, some of them with important invasive potential, belonging mainly to families Lumbricidae, Acanthodrilidae, Benhamiidae, Megascolecidae, Rhinodrilidae and Ocnerodrilidae. Taxonomic housekeeping was performed for the preoccupied Rhinodrilidae genus Tairona Righi - herein substituted by Taironina nom. nov., and Guarani camaqua Rodríguez & Lima was reinstated and removed from synonymy with Criodrilus lacuum Hoffmeister, 1845, resulting in a wider definition of the Almidae family. Furthermore, Amynthas maximalis nom. nov. is proposed herein as a substitution name for the preoccupied name Amynthas maximus Qiu & Dong, 2019, and Arecoidae is proposed herein as a new monotypic family for the aquamegadrile species Areco reco Righi, Ayres & Bittencourt, 1978.

Distribution and diversity of earthworms in different land use systems in Rio Grande do Sul, Brazil.

The southernmost state of Brazil, Rio Grande do Sul, includes both the Pampa and Atlantic Forest biomes, and covers an area of around 284 thousand km2. Since the last checklist for the state, published in 2012, many additional sites have been sampled. Hence, we provide here a full list of earthworm species and their distribution in different municipalities and land use systems in the state. Data were obtained from the literature, museums, and other earthworm collections and recent sampling efforts. Records for 19 additional species are reported here for the first time, raising the total to 78 species/subspecies currently known from Rio Grande do Sul, from the families Acanthodrilidae (5), Benhamiidae (3), Lumbricidae (13), Megascolecidae (11), Ocnerodrilidae (22), Almidae (2), Glossoscolecidae (18) and Rhinodrilidae (5). Alexidrilus Righi, 1971 is synonymized with Urobenus Benham, 1886, as both species in the genus (A. littoralis Ljungström, 1972 and A. lourdesae Righi, 1971) have similar morphology to the type species Urobenus brasiliensis Benham, 1886. Most of the native species/subspecies in the state (39), including an important number of new species, were found in native vegetation with lesser disturbance, such as swamps, native forests and pastures. On the other hand, the three peregrine and 27 exotic species (especially Pontoscolex corethrurus, Eukerria spp., lumbricids, megascolecids, Dichogaster spp., and Microscolex spp.) dominated in disturbed sites, such as compost piles, urban areas, rice and other crops, and cultivated pastures. Considering the geographic extension and the many unsampled sites in the state, further collection efforts are necessary and will certainly reveal new species, as evidenced here.

Earthworms in natural grasslands and agropastoral systems in the Brazilian Pampa.

In this study, we sampled grazed natural grassland and agropastoral systems in two regions (Alegrete and Aceguá) in the highly diverse Brazilian Pampa region which is undergoing significant change in land use cover. By hand sorting the soil, a total of five species were found, belonging to the families Glossoscolecidae (Glossoscolex sp.50 and Glossoscolex sp.51), Ocnerodrilidae (Kerriona sp.6 and Eukerria sp.3), and Lumbricidae (Aporrectodea trapezoides). The former four were identified as native-new to science-and the latter as exotic. Differences were observed in both earthworm species richness and abundance between regions and land-use systems, likely due to environmental conditions and agricultural practices, respectively. Agropastoral systems had higher abundance and species richness than natural grasslands, though these differences varied by location. This suggests that conversion from natural grassland to agropastoral systems can maintain native earthworms and, possibly, enhance ecosystem services. However, an exotic species was found in high abundance at one agropastoral site. These results highlight the need for additional biodiversity studies on earthworms in the Brazilian Pampa and for research on the impacts of land use changes in this region.

Earthworm richness in no-tillage farming systems and riparian forests in Southern and Southeastern Brazil.

No-tillage (NT) is a soil conservation management practice that can sustain important earthworm populations due the maintenance of soil cover and absence of soil disturbance. Sites with NT can also support native earthworm species, but few studies have identified the earthworms collected under NT. Therefore, the present study evaluated earthworm diversity in 14 sites under NT and seven with riparian forest (RF) in Atlantic Forest located in Rolândia, Cambé, Toledo (Paraná), Coxilha, Sarandi (Rio Grande do Sul) and Itaí (São Paulo). The earthworms were collected by handsorting using quantitative and qualitative methods. The 1471 individuals collected were classified into 23 species belonging to five families: Glossoscolex sp.71, sp.72, sp.73 and sp.74, Fimoscolex sp.23 sp.24 and sp.25 (Glossoscolecidae); Pontoscolex corethrurus, Urobenus brasiliensis (Rhinodrilidae); Haplodrilus sp.1 and sp.2, Ocnerodrilidae sp.1, sp.15, sp.16, sp.48, sp.49 and sp.50 (Ocnerodrilidae); Amynthas gracilis, Metaphire californica (Megascolecidae); Dichogaster affinis, D. gracilis, D. bolaui and D. saliens (Benhamiidae). Of the total, 15 were native and eight were exotic species. In general NT sites had higher species richness than the forest sites (except at Itaí), mainly due to the invasion of NT fields by exotic species, particularly Ocnerodrilidae sp.1 and Dichogaster spp. Six of the seven forests had native species, but only four NT fields had native earthworms, and these were generally found in low abundance.

Earthworm communities in long-term no-tillage systems and secondary forest fragments in Paraná, Southern Brazil.

The area destined for agricultural production in Paraná state in Brazil is ~6 million hectares, of which 79% are under no-tillage systems (NTS) that can positively affect earthworm populations. Furthermore, earthworm abundance and richness can be valuable soil quality. This study assessed earthworm communities in long-term no-tillage sites (NTS) and nearby secondary Atlantic Forest (SF) fragments. Sampling was performed in June 2018 and May 2019 using the quantitative Tropical Soil Biology and Fertility handsorting method (ISO-TSBF), complemented by qualitative sampling in three municipalities: Faxinal, Mauá da Serra and Palmeira, in NTS and SF. Eleven earthworm species, belonging to five families (Benhamiidae, Glossoscolecidae, Megascolecidae, Ocnerodrilidae, and Rhinodrilidae) were found along with seven native species (Glossoscolex sp.22, Glossoscolex sp.23, Fimoscolex sp.21, Fimoscolex sp.24, Fimoscolex sp.42, Andiorrhinus duseni and Urobenus brasiliensis) and four exotic or cosmopolitan species (Dichogaster gracilis, Amynthas gracilis, Metaphire californica and Pontoscolex corethrurus). The cosmopolitan P. corethrurus dominated Faxinal and Mauá da Serra, while in Palmeira Fimoscolex and Glossoscolex were the most abundant. Six species belonging to Glossoscolex and Fimoscolex were new to science and must be described. Overall, 239 individuals were found. In 2018 126 individuals were found (76 in NTS and 50 in SF) and in 2019, only 112 individuals were found (45 in NTS and 67 in SF). The highest earthworm abundance was in Faxinal (123 individuals), with 76 individuals in NTS and 47 in SF. In Mauá da Serra the same pattern was observed (SF>NTS), while in Palmeira in overall, fewer individuals (38) were collected (24 in NTS and 14 in SF). The NTS at Faxinal and SF Mauá da Serra in 2019 had only/more exotic/cosmopolitan species, while at the other dates and sites >50% of the specimens sampled were native species. Overall, forests had more native species than exotics: in 2018, natives represented > 75% of all specimens, but in 2019 both Faxinal and Mauá da Serra had more exotic species (> 65%), while at Palmeira, they represented 50% of the individuals. Earthworm total biomass for forest sites was higher in Faxinal (3.2 g) and NTS in Mauá da Serra (1.7 g) in 2019. Overall, the total biomass was higher in SF sites than NTS. Using a classification available for earthworm populations in no-tillage systems, all three sites were considered to have poor quality in terms of abundance (ind m-2), while for species richness, they were considered to have moderate (Faxinal and Mauá da Serra) and good quality (Palmeira).

Urban green areas as earthworm species maintainers in Curitiba, Paraná, Brazil.

Urban green areas can have a high impact on soil fauna due the environmental changes caused by human activities. This work aimed to assess earthworm populations in urban sites in the city of Curitiba, Paraná, Brazil. Earthworms were sampled in two urban sites: the Campus Ecoville of the Universidade Positivo and the Casa Verde space and in two land use systems (Grass Lawn and Native Vegetation), in the summer of 2018 and 2019 and winter of 2019. A total of 1052 individuals were found, belonging to five families and 12 species were identified, of which five are native: Glossoscolex sp.55, Fimoscolex sp.36, Fimoscolex sp.37 (Glossoscolecidae), Andiorrhinus duseni (Rhinodrilidae) and Ocnerodrilidae sp.43 (Ocnerodrilidae) and seven are exotic species: Amynthas corticis, Amynthas morrisi, Metaphire californica, Metaphire schmardae (Megascolecidae), Dendrodrilus rubidus (Lumbricidae) and Pontoscolex corethrurus (Rhinodrilidae). Of the five native species found, four are new species and will be described in the future and the specie A. duseni belongs to the group known as giant earthworms (minhocuçu) with specimens larger than 30cm. Despite the predominance of exotic species (n=932) compared to native species (n=114), the presence of new species may indicate that the managed environments can preserve native species. Moreover, the difference in the total number (native vs. exotic) can be attributed to the ability of the exotic species to survive human disturbances.

Earthworms in various agricultural and forest ecosystems in São Carlos-SP, Brazil.

Integrated agricultural systems have been greatly expanding in Brazil and currently cover over 30 million hectares. Although these land use systems help soil and environmental quality, little is known of their impacts on earthworm communities. In the present study we evaluated earthworm abundance, biomass and species richness in various integrated and conventional agricultural production systems (pasture, crops), as well as native forest and Eucalyptus plantations. The research was carried out at Fazenda Canchim (Embrapa Livestock Southeast) and at the neighboring campus of the University de São Carlos (UFSCar), in São Carlos municipality in São Paulo state, Brazil. Two earthworm sampling methods, i.e., quantitative (25 x 25 x 20 cm deep monoliths) and qualitative (holes of similar size and niche sampling), were used in three integrated production systems, three conventional systems and two forest systems, totaling 232 quantitative samples and over 160 qualitative samples. Earthworms were counted and identified using available keys. Eight earthworm species of five families (Rhinodrilidae, Ocnerodrilidae, Megascolecidae, Benhamiidae and Glossoscolecidae) were found, with most of the 2,145 individuals being exotic or peregrine species, particularly Pontoscolex corethrurus (88% of total) and various Dichogaster spp. (8% of total). Two native species were encountered in low abundance (55 individuals), both of which are new to science (Fimoscolex n.sp.7 and Glossoscolex n.sp.9) and must still be formally described. The new species were found in the agricultural systems, but not in the native forest and the Eucalyptus plantation, where P. corethrurus and Megascolecids predominated. One unidentified Ocnerodrilid sp. was found only in the intensive pasture. Species richness was highest in the intensive pasture (7 spp.), followed by the agrosilvopastoral and agropastoral systems, highlighting the importance of integrated and pastoral systems in preserving earthworm richness and native species.

Earthworm species in native and planted forests in Brazil.

Over 150 species of earthworms are known from the Brazilian Atlantic rainforest, but many more are expected to live in this megadiverse biome. In the present study, we evaluated earthworm species occurrence and diversity in native and reforested areas in four National Forests in three Brazilian states: Três Barras National Forest (Santa Catarina), Irati and Piraí do Sul National Forests (Paraná) and Capão Bonito National Forest (São Paulo) using formalin and hand sorting methods. A total of 13 species were found, five exotic and eight natives (of which four were new, undescribed species), belonging to six genera and five families (Rhinodrilidae, Glossoscolecidae, Ocnerodrilidae, Benhamiidae, Megascolecidae). In general, higher number of earthworms were collected in Araucaria plantations, except in Capão Bonito where Pinus sp. plantations had higher abundance. Hand sorting also collected more species in all forests, so this should be the preferred sampling method to evaluate earthworm communities. Formalin extraction was efficient only for epi-endogeic earthworms, most of them exotic species. Exotic pine species plantations tend to reduce species richness and abundance, compared with native forests and Araucaria plantations, and substitution of native vegetation for pine plantations should consider potential negative effects on soil animal populations.

Earthworm species in different land use systems in the state of Goiás and the Federal District of Brazil.

The state of Goiás and the Federal District together cover an area of about 346 thousand km2, including the diversified Cerrado vegetation, also known as the Brazilian Savanna, a biodiversity hotspot with many endangered and endemic species. Much of the Cerrado has been converted to agriculture, but little is known of its earthworms, and the impacts of land use management and agricultural practices on their density and diversity. In the present paper we review the known earthworm species in Goiás and the Federal District based on historical and museum records, and update information on their distribution and populations in different counties and land use systems with data from recent samplings. Five additional species are reported here for the first time, raising the total to 12 species currently known from Goiás, from the families Benhamiidae (1), Glossoscolecidae (1), Megascolecidae (3), Ocnerodrilidae (2) and Rhinodrilidae (5). In the Federal District, an additional 10 species are reported, bringing the total to 23 earthworm species, belonging to the families Benhamiidae (4), Eudrilidae (1), Glossoscolecidae (2), Ocnerodrilidae (1), Lumbricidae (2), Megascolecidae (1) and Rhinodrilidae (12). Native species in Goiás (6) were found in areas with lesser disturbance, such as in native forest and flooded areas/river banks, a phenomenon also observed in the Federal District. On the other hand, the exotic and peregrine species found in Goiás (6) and in the Federal District (9) dominated in disturbed sites, such as perennial crops, agroforestry, annual crops, urban areas, secondary forests, pastures and agropastoral systems, among others. Given the many new species found and the extension of Goiás and the Federal District, further effort is needed to adequately characterize the earthworm fauna of the region, from where many other species are expected.

Earthworms from natural and managed ecosystems in Southern Bahia, Brazil.

The earthworms (Crassiclitellata) of the Northern Atlantic Forest region are poorly known, and many new species are expected to be found in the region. Hence, quantitative and qualitative samples were taken in the Atlantic Forest region of southern Bahia State, Brazil, at eight sites to assess species presence and abundance in different land uses (forests, pastures, plantations). Earthworms were also qualitatively collected at an additional eight sites. Only one to two species per site were found in quantitative samples, while qualitative samples generally resulted in higher species recoveries, with highest richness observed at the native Atlantic Forest of Veracel in Porto Seguro. Pontoscolex corethrurus (Müller, 1857) was the dominant species at all sites and in most land use systems, probably favored by human migration and agricultural activities; it was only absent in one primary forest site where native Ocnerodrilidae species predominated. In total at least 14 species were found of which eight were new, belonging to the genus Rhinodrilus and three new genera in the Ocnerodrilidae family (Pauqueba, Parabauba and Diplomoela). Contrary to sites in Southern and Southeastern Brazil, where many native species belong to the Glossoscolecidae family, in southern Bahia, Ocnerodrilidae species tend to be more prevalent, occupying various habitats. More intensive sampling of these habitats, including surface litter, in and under fallen logs and dead tree trunks, under rocks, in bromeliad leaf tanks and deeper soil layers is essential in order to properly characterize earthworm communities in Brazilian Atlantic Forests.