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Parascedosporium putredinis NO1 is a plant biomass-degrading ascomycete with a propensity to target the most recalcitrant components of lignocellulose. Here we applied proteomics and activity-based protein profiling (ABPP) to investigate the ability of P. putredinis NO1 to tailor its secretome for growth on different lignocellulosic substrates. Proteomic analysis of soluble and insoluble culture fractions following the growth of P. putredinis NO1 on six lignocellulosic substrates highlights the adaptability of the response of the P. putredinis NO1 secretome to different substrates. Differences in protein abundance profiles were maintained and observed across substrates after bioinformatic filtering of the data to remove intracellular protein contamination to identify the components of the secretome more accurately. These differences across substrates extended to carbohydrate-active enzymes (CAZymes) at both class and family levels. Investigation of abundant activities in the secretomes for each substrate revealed similar variation but also a high abundance of "unknown" proteins in all conditions investigated. Fluorescence-based and chemical proteomic ABPP of secreted cellulases, xylanases, and ß-glucosidases applied to secretomes from multiple growth substrates for the first time confirmed highly adaptive time- and substrate-dependent glycoside hydrolase production by this fungus. P. putredinis NO1 is a promising new candidate for the identification of enzymes suited to the degradation of recalcitrant lignocellulosic feedstocks. The investigation of proteomes from the biomass bound and culture supernatant fractions provides a more complete picture of a fungal lignocellulose-degrading response. An in-depth understanding of this varied response will enhance efforts toward the development of tailored enzyme systems for use in biorefining.IMPORTANCEThe ability of the lignocellulose-degrading fungus Parascedosporium putredinis NO1 to tailor its secreted enzymes to different sources of plant biomass was revealed here. Through a combination of proteomic, bioinformatic, and fluorescent labeling techniques, remarkable variation was demonstrated in the secreted enzyme response for this ascomycete when grown on multiple lignocellulosic substrates. The maintenance of this variation over time when exploring hydrolytic polysaccharide-active enzymes through fluorescent labeling, suggests that this variation results from an actively tailored secretome response based on substrate. Understanding the tailored secretomes of wood-degrading fungi, especially from underexplored and poorly represented families, will be important for the development of effective substrate-tailored treatments for the conversion and valorization of lignocellulose.
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Proteínas Fúngicas , Lignina , Proteômica , Lignina/metabolismo , Proteínas Fúngicas/metabolismo , Secretoma/metabolismo , Biomassa , Celulases/metabolismo , Ascomicetos/metabolismo , Ascomicetos/crescimento & desenvolvimento , Ascomicetos/enzimologiaRESUMO
Gold nanoparticles (Au-NPs) are used as catalysts for a diverse range of industrial applications. Currently, Au-NPs are synthesized chemically, but studies have shown that plants fed Au deposit, this element naturally as NPs within their tissues. The resulting plant material can be used to make biomass-derived catalysts. In vitro studies have shown that the addition of specific, short (â¼10 amino acid) peptide/s to solutions can be used to control the NP size and shape, factors that can be used to optimize catalysts for different processes. Introducing these peptides into the model plant species, Arabidopsis thaliana (Arabidopsis), allows us to regulate the diameter of nanoparticles within the plant itself, consequently influencing the catalytic performance in the resulting pyrolyzed biomass. Furthermore, we show that overexpressing the copper and gold COPPER TRANSPORTER 2 (COPT2) in Arabidopsis increases the uptake of these metals. Adding value to the Au-rich biomass offers the potential to make plant-based remediation and stabilization of mine wastes financially feasible. Thus, this study represents a significant step toward engineering plants for the sustainable recovery of finite and valuable elements from our environment.
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Arabidopsis , Ouro , Nanopartículas Metálicas , Ouro/química , Nanopartículas Metálicas/química , Arabidopsis/metabolismo , Catálise , Biomassa , Tamanho da Partícula , Cobre/químicaRESUMO
Realising a fully circular bioeconomy requires the valorisation of lignocellulosic biomass. Cellulose is the most attractive component of lignocellulose but depolymerisation is inefficient, expensive and resource intensive requiring substantial volumes of potable water. Seawater is an attractive prospective replacement, however seawater tolerant enzymes are required for the development of seawater-based biorefineries. Here, we report a halophilic cellobiohydrolase SMECel6A, identified and isolated from a salt marsh meta-exo-proteome dataset with high sequence divergence to previously characterised cellobiohydrolases. SMECel6A contains a glycoside hydrolase family 6 (GH6) domain and a carbohydrate binding module family 2 (CBM2) domain. Characterisation of recombinant SMECel6A revealed SMECel6A to be active upon crystalline and amorphous cellulose. Mono- and oligosaccharide product profiles revealed cellobiose as the major hydrolysis product confirming SMECel6A as a cellobiohydrolase. We show SMECel6A to be halophilic with optimal activity achieved in 0.5X seawater displaying 80.6 ± 6.93% activity in 1 × seawater. Structural predictions revealed similarity to a characterised halophilic cellobiohydrolase despite sharing only 57% sequence identity. Sequential thermocycling revealed SMECel6A had the ability to partially reversibly denature exclusively in seawater retaining significant activity. Our study confirms that salt marsh ecosystems harbour enzymes with attractive traits with biotechnological potential for implementation in ionic solution based bioprocessing systems.
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Celulose 1,4-beta-Celobiosidase , Glicosídeo Hidrolases , Celulose 1,4-beta-Celobiosidase/metabolismo , Áreas Alagadas , Ecossistema , Estudos Prospectivos , Celulose/metabolismo , Água do MarRESUMO
We present an optimal configuration for Stokes polarimeters based on liquid crystal variable retarders, with the minimum number of measurements. Due to the inherent variations of the director orientation of the liquid crystal molecules, we propose a configuration that minimizes the sensibility of the polarimeter to fast-axis variations. For the optimization we consider a scheme that maximizes the volume of a tetrahedron inscribed in the Poincare sphere, to address additive and Poisson noise, with one of the vertices invariant to changes in the axis positions. We provide numerical simulations, considering misalignment errors, to analyze the robustness of the configuration. The results show that the proposed configuration helps to maintain the volume enclosed by the tetrahedron with high tolerance to fast-axis orientation errors. The condition number will remain below 3.07 for common misalignment errors and below 1.88 for more controlled liquid crystals. This optimization will improve the performance of liquid crystals polarimeters, with a more robust configuration that also considers misalignment errors, beyond additive and Poisson noise.
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The use of polarization measurements has become more common in recent years, as it gives more information than pure intensity measurements. Polarimetric components such as fixed or variable retarders and polarizers must be included in optical systems to obtain the polarization parameters required, and in many cases the optical system also includes other components such as relay and/or imaging optical systems. In this work we present a simple and robust method for the polarimetric characterization of non-depolarizing polarization components and other optical elements in the system, which does not require a full polarimeter. Since there is no depolarization, we represent the components as pure retarders with diattenuation and find their parameters (transmittance for the polarization components, angle of orientation of the fast axis, and retardance), from which we can retrieve their Mueller matrix. Our results show that the proposed method is accurate when compared with results obtained with a Mueller matrix dual-rotating retarder polarimeter calibrated using the eigenvalue calibration method, considered in this work as the gold standard, and is comparatively easier than the latter to implement, particularly for imaging polarimeters.
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Head rotation in human spermatozoa is essential for different swimming modes and fertilisation, as it links the molecular workings of the flagellar beat with sperm motion in three-dimensional (3D) space over time. Determining the direction of head rotation has been hindered by the symmetry and translucent nature of the sperm head, and by the fast 3D motion driven by the helical flagellar beat. Analysis has been mostly restricted to two-dimensional (2D) single focal plane image analysis, which enables tracking of head centre position but not tracking of head rotation. Despite the conserved helical beating of the human sperm flagellum, human sperm head rotation has been reported to be uni- or bi-directional, and even to intermittently change direction in a given cell. Here, we directly measure the head rotation of freely swimming human sperm using multi-plane 4D (3D+t) microscopy and show that: (1) 2D microscopy is unable to distinguish head rotation direction in human spermatozoa; (2) head rotation direction in non-capacitating and capacitating solutions, for both aqueous and viscous media, is counterclockwise (CCW), as seen from head to tail, in all rotating spermatozoa, regardless of the experimental conditions; and (3) head rotation is suppressed in 36% of spermatozoa swimming in non-capacitating viscous medium, although CCW rotation is recovered after incubation in capacitating conditions within the same viscous medium, possibly unveiling an unexplored aspect of the essential need of capacitation for fertilisation. Our observations show that the CCW head rotation in human sperm is conserved. It constitutes a robust and persistent helical driving mechanism that influences sperm navigation in 3D space over time, and thus is of critical importance in cell motility, propulsion of flagellated microorganisms, sperm motility assessments, human reproduction research, and self-organisation of flagellar beating patterns and swimming in 3D space.
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Motilidade dos Espermatozoides , Natação , Humanos , Masculino , Sêmen , Espermatozoides , Cauda do EspermatozoideRESUMO
IMPORTANCE: An annotated reference genome has revealed P. putredinis NO1 as a useful resource for the identification of new lignocellulose-degrading enzymes for biorefining of woody plant biomass. Utilizing a "structure-omics"-based searching strategy, we identified new potentially lignocellulose-active sequences that would have been missed by traditional sequence searching methods. These new identifications, alongside the discovery of novel enzymatic functions from this underexplored lineage with the recent discovery of a new phenol oxidase that cleaves the main structural ß-O-4 linkage in lignin from P. putredinis NO1, highlight the underexplored and poorly represented family Microascaceae as a particularly interesting candidate worthy of further exploration toward the valorization of high value biorenewable products.
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Ascomicetos , Lignina , Lignina/metabolismo , Ascomicetos/genética , Ascomicetos/metabolismo , Estresse OxidativoRESUMO
The increasing availability of microbial genome sequences provides a reservoir of information for the identification of new microbial enzymes. Genes encoding proteins engaged in extracellular processes are of particular interest as these mediate the interactions microbes have with their environments. However, proteomic analysis of secretomes is challenging and often captures intracellular proteins released through cell death and lysis. Secretome prediction workflows from sequence data are commonly used to filter proteins identified through proteomics but are often simplified to a single step and are not evaluated bioinformatically for their effectiveness. Here, a workflow to predict a fungal secretome was designed and applied to the coding regions of the Parascedosporium putredinis NO1 genome. This ascomycete fungus is an exceptional lignocellulose degrader from which a new lignin-degrading enzyme has previously been identified. The 'secretome isolation' workflow is based on two strategies of localisation prediction and secretion prediction each utilising multiple available tools. The workflow produced three final secretomes with increasing levels of stringency. All three secretomes showed increases in functional annotations for extracellular processes and reductions in annotations for intracellular processes. Multiple sequences isolated as part of the secretome lacked any functional annotation and made exciting candidates for novel enzyme discovery.
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Ascomicetos , Lignina , Lignina/metabolismo , Secretoma , Fluxo de Trabalho , Proteômica , Ascomicetos/genética , Ascomicetos/metabolismoRESUMO
In this paper, we study two configurations of a full-Stokes polarimeter that measures three wavelengths simultaneously. A recently developed fitting calibration process is used to reduce the error in the final Stokes vector. The first configuration uses an optimized setup for the central wavelength that fixes the non-optimized setups for the other two wavelengths. The second configuration is the result of a search for the best simultaneous setups for all three wavelengths. It is found that this second setup gives smaller errors in the measured Stokes parameters before calibration, but the first configuration gives smaller errors after calibration. These results demonstrate the need for a method of finding the best polarimeter configuration for this type of measurement. It seems clear that the condition number by itself is not a metric that provides sufficient information to determine whether a particular configuration is better by giving smaller errors. It is also important to point out that the results presented here are for the particular calibration scheme used. Additionally, it may be that a different calibration method is required to give better results, but that is outside the scope of the present work.
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Robertkochia solimangrovi is a proposed marine bacterium isolated from mangrove soil. So far, the study of this bacterium is limited to taxonomy only. In this report, we performed a genomic analysis of R. solimangrovi that revealed its lignocellulose degrading ability. Genome mining of R. solimangrovi revealed a total of 87 lignocellulose degrading enzymes. These enzymes include cellulases (GH3, GH5, GH9 and GH30), xylanases (GH5, GH10, GH43, GH51, GH67, and GH115), mannanases (GH2, GH26, GH27 and GH113) and xyloglucanases (GH2, GH5, GH16, GH29, GH31 and GH95). Most of the lignocellulolytic enzymes encoded in R. solimangrovi were absent in the genome of Robertkochia marina, the closest member from the same genus. Furthermore, current work also demonstrated the ability of R. solimangrovi to produce lignocellulolytic enzymes to deconstruct oil palm empty fruit bunch (EFB), a lignocellulosic waste found abundantly in palm oil industry. The metabolic pathway taken by R. solimangrovi to transport and process the reducing sugars after the action of lignocellulolytic enzymes on EFB was also inferred based on genomic data. Collectively, genomic analysis coupled with experimental studies elucidated R. solimangrovi to serve as a promising candidate in seawater based-biorefinery industry.
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Celulases , Lignina , Lignina/metabolismo , Celulases/genética , Óleo de Palmeira , Bactérias/metabolismo , GenômicaRESUMO
Spectrally modulated Stokes polarimeters use a pair of high-order crystal retarders to generate a spectrally dependent modulation of the polarization of light. In these systems, the detected intensity versus wavenumber spectrum is usually referred to as a channeled spectrum, and the Fourier inversion of this spectrum allows the determination of Stokes parameters of light without needing any other mechanical or active device for polarization control. This work proposes a spectrally modulated polarimeter beyond the concept of a channeled wavenumber spectrum, so effectively detaching the spectral modulation from the Fourier analysis technique. The wavelength domain analysis we use is best suited for dispersive spectrometers offering intensity versus wavelength measurements. The technique is illustrated with the measurement of very small optical rotations produced by sucrose solutions. The proposed technique is easily extendable to spectrally modulated Mueller polarimeters.
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Engineered plants can clean up pollution and recover technology-critical metals.
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Poluição Ambiental , Recuperação e Remediação Ambiental , Metais Pesados , Poluentes Orgânicos Persistentes , Plantas Geneticamente Modificadas , Poluentes do Solo , Poluição Ambiental/prevenção & controle , Recuperação e Remediação Ambiental/métodos , Metais Pesados/metabolismo , Poluentes Orgânicos Persistentes/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Poluentes do Solo/metabolismoRESUMO
There is an urgent need to replace petroleum-based plastic with bio-based and biodegradable alternatives. Polyhydroxyalkanoates (PHAs) are attractive prospective replacements that exhibit desirable mechanical properties and are recyclable and biodegradable in terrestrial and marine environments. However, the production costs today still limit the economic sustainability of the PHA industry. Seaweed cultivation represents an opportunity for carbon capture, while also supplying a sustainable photosynthetic feedstock for PHA production. We mined existing gene and protein databases to identify bacteria able to grow and produce PHAs using seaweed-derived carbohydrates as substrates. There were no significant relationships between the genes involved in the deconstruction of algae polysaccharides and PHA production, with poor to negative correlations and diffused clustering suggesting evolutionary compartmentalism. We identified 2â987 bacterial candidates spanning 40 taxonomic families predominantly within Alphaproteobacteria, Gammaproteobacteria and Burkholderiales with enriched seaweed-degrading capacity that also harbour PHA synthesis potential. These included highly promising candidates with specialist and generalist specificities, including Alteromonas, Aquisphaera, Azotobacter, Bacillus, Caulobacter, Cellvibrionaceae, Duganella, Janthinobacterium, Massilia, Oxalobacteraceae, Parvularcula, Pirellulaceae, Pseudomonas, Rhizobacter, Rhodanobacter, Simiduia, Sphingobium, Sphingomonadaceae, Sphingomonas, Stieleria, Vibrio and Xanthomonas. In this enriched subset, the family-level densities of genes targeting green macroalgae polysaccharides were considerably higher (n=231.6±68.5) than enzymes targeting brown (n=65.34±13.12) and red (n=30.5±10.72) polysaccharides. Within these organisms, an abundance of FabG genes was observed, suggesting that the fatty acid de novo synthesis pathway supplies (R)-3-hydroxyacyl-CoA or 3-hydroxybutyryl-CoA from core metabolic processes and is the predominant mechanism of PHA production in these organisms. Our results facilitate extending seaweed biomass valorization in the context of consolidated biorefining for the production of bioplastics.
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Petróleo , Poli-Hidroxialcanoatos , Alga Marinha , Bactérias/genética , Bactérias/metabolismo , Carboidratos , Carbono/metabolismo , Coenzima A/metabolismo , Ácidos Graxos/metabolismo , Humanos , Petróleo/metabolismo , Plásticos/metabolismo , Poli-Hidroxialcanoatos/química , Poli-Hidroxialcanoatos/metabolismo , Estudos ProspectivosRESUMO
The transmission properties of a photonic crystal immersed in several different oils have been characterized using terahertz time domain spectroscopy in the spectral range of 0.3-1.5 THz. As in previous works, oil samples can be distinguished using terahertz transmission measurements. When the same oils are introduced into a photonic crystal, we find that the effective refractive index of the photonic crystal is sensitive to the properties of the oils and shows differences not seen in bulk measurements. These effects are described in detail and have potential applications in both the sensing of very small volumes of oils and in the fine control of the refractive indices of photonic crystals.
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Liquid crystal variable retarders (LCVRs) are often used in Stokes polarimeters as they allow the measurement of different polarization components by applying an electric field that manipulates the induced retardance. However, the optical retardance introduced by these devices is in general not homogenous across the aperture. Another problem with this type of devices is that the fast-axis orientation is not homogenous, and it changes with the applied voltage. For the optimization of polarimeters, in terms of the noise amplification from the intensity measurements to the polarimetric data, the condition number (CN) is often used, but the effects of LCVR spatial variations are not considered. This paper analyzes the impact of errors in LCVRs in a set of optimized Stokes polarimeters simulated by adding errors in the induced retardance and fast-axis orientation. Then, the CN is calculated to observe the effect of these errors on the optimization. We show how errors in the LCVRs lead to different impacts in the polarimetric measurements for different optimized polarimeters, depending on their experimental parameters. Furthermore, we present the propagation error theory to choose the best experimental parameters to reduce the nonideal effects in optimized polarimeters.
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BACKGROUND: Shipworms are marine xylophagus bivalve molluscs, which can live on a diet solely of wood due to their ability to produce plant cell wall-degrading enzymes. Bacterial carbohydrate-active enzymes (CAZymes), synthesised by endosymbionts living in specialised shipworm cells called bacteriocytes and located in the animal's gills, play an important role in wood digestion in shipworms. However, the main site of lignocellulose digestion within these wood-boring molluscs, which contains both endogenous lignocellulolytic enzymes and prokaryotic enzymes, is the caecum, and the mechanism by which bacterial enzymes reach the distant caecum lumen has remained so far mysterious. Here, we provide a characterisation of the path through which bacterial CAZymes produced in the gills of the shipworm Lyrodus pedicellatus reach the distant caecum to contribute to the digestion of wood. RESULTS: Through a combination of transcriptomics, proteomics, X-ray microtomography, electron microscopy studies and in vitro biochemical characterisation, we show that wood-digesting enzymes produced by symbiotic bacteria are localised not only in the gills, but also in the lumen of the food groove, a stream of mucus secreted by gill cells that carries food particles trapped by filter feeding to the mouth. Bacterial CAZymes are also present in the crystalline style and in the caecum of their shipworm host, suggesting a unique pathway by which enzymes involved in a symbiotic interaction are transported to their site of action. Finally, we characterise in vitro four new bacterial glycosyl hydrolases and a lytic polysaccharide monooxygenase identified in our transcriptomic and proteomic analyses as some of the major bacterial enzymes involved in this unusual biological system. CONCLUSION: Based on our data, we propose that bacteria and their enzymes are transported from the gills along the food groove to the shipworm's mouth and digestive tract, where they aid in wood digestion.
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Bivalves , Proteômica , Animais , Bactérias , Filogenia , SimbioseRESUMO
The oomycete Phytophthora infestans is a damaging crop pathogen and a model organism to study plant-pathogen interactions. We report the discovery of a family of copper-dependent lytic polysaccharide monooxygenases (LPMOs) in plant pathogenic oomycetes and its role in plant infection by P. infestans We show that LPMO-encoding genes are up-regulated early during infection and that the secreted enzymes oxidatively cleave the backbone of pectin, a charged polysaccharide in the plant cell wall. The crystal structure of the most abundant of these LPMOs sheds light on its ability to recognize and degrade pectin, and silencing the encoding gene in P. infestans inhibits infection of potato, indicating a role in host penetration. The identification of LPMOs as virulence factors in pathogenic oomycetes opens up opportunities in crop protection and food security.
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Oxigenases de Função Mista/metabolismo , Pectinas/metabolismo , Phytophthora infestans/enzimologia , Doenças das Plantas/parasitologia , Solanum lycopersicum/parasitologia , Solanum tuberosum/parasitologia , Cobre , Oxigenases de Função Mista/química , Oxigenases de Função Mista/genética , Modelos Moleculares , Oxirredução , Phytophthora infestans/genética , Phytophthora infestans/patogenicidade , Folhas de Planta/parasitologia , Polissacarídeos/metabolismo , Conformação Proteica , Domínios Proteicos , Fatores de Virulência/química , Fatores de Virulência/genética , Fatores de Virulência/metabolismoRESUMO
The oomycete pathogen Aphanomyces astaci, also known as "crayfish plague", is an obligate fungal-like parasite of freshwater crustaceans and is considered responsible for the ongoing decline of native European crayfish populations. A. astaci is thought to secrete a wide array of effectors and enzymes that facilitate infection, however their molecular mechanisms have been poorly characterized. Here, we report the identification of AA15 lytic polysaccharide monooxygenases (LPMOs) as a new group of secreted virulence factors in A. astaci. We show that this enzyme family has greatly expanded in A. astaci compared to all other oomycetes, and that it may facilitate infection through oxidative degradation of crystalline chitin, the most abundant polysaccharide found in the crustacean exoskeleton. These findings reveal new roles for LPMOs in animal-pathogen interactions, and could help inform future strategies for the protection of farmed and endangered species.
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Doenças dos Animais/microbiologia , Aphanomyces , Astacoidea/microbiologia , Infecções , Oxigenases de Função Mista/metabolismo , Fatores de Virulência/metabolismo , Animais , Aphanomyces/enzimologia , Aphanomyces/patogenicidade , Quitina/metabolismo , Infecções/microbiologia , Infecções/veterináriaRESUMO
This work analyzes the effect of experimental errors and measurement noise on Mueller matrix channeled spectropolarimeters. The main advantage of this type of polarimeter is the independence on temporal resolution, as it can be used as a snapshot polarimeter. The simulation of the polarimeters with experimental errors and two published extraction methods of the sample Mueller matrix are also presented. The Mueller matrix channeled spectropolarimeter (MMCS) setup consists of a mirrored Stokes channeled spectropolarimeter (SCS) as the polarization states generator (PSG) and a SCS as the polarization states analyzer (PSA). The SCS setup comprises two thick birefringent retarders followed by a horizontal linear polarizer. The effects of the thickness ratio of the retarders, the global retardance factor, retardance errors, axes alignment errors, and additive Gaussian noise are further studied to optimize the MMCS setups. In this work, we do not include a calibration procedure to improve the measured Mueller matrix parameters, but we study the sensitivity of the polarimeter to different configurations and error sources.