RESUMO
Peptidoglycan (PG), an essential structure in the cell walls of the vast majority of bacteria, is critical for division and maintaining cell shape and hydrostatic pressure. Bacteria comprising the Chlamydiales were thought to be one of the few exceptions. Chlamydia harbour genes for PG biosynthesis and exhibit susceptibility to 'anti-PG' antibiotics, yet attempts to detect PG in any chlamydial species have proven unsuccessful (the 'chlamydial anomaly'). We used a novel approach to metabolically label chlamydial PG using d-amino acid dipeptide probes and click chemistry. Replicating Chlamydia trachomatis were labelled with these probes throughout their biphasic developmental life cycle, and the results of differential probe incorporation experiments conducted in the presence of ampicillin are consistent with the presence of chlamydial PG-modifying enzymes. These findings culminate 50 years of speculation and debate concerning the chlamydial anomaly and are the strongest evidence so far that chlamydial species possess functional PG.
Assuntos
Parede Celular/química , Parede Celular/metabolismo , Chlamydia trachomatis/química , Peptidoglicano/análise , Coloração e Rotulagem/métodos , Aminoácidos/química , Aminoácidos/metabolismo , Chlamydia trachomatis/citologia , Chlamydia trachomatis/efeitos dos fármacos , Chlamydia trachomatis/metabolismo , Química Click , Dipeptídeos/análise , Dipeptídeos/química , Fluorescência , Espaço Intracelular/química , Espaço Intracelular/metabolismo , Sondas Moleculares/análise , Sondas Moleculares/química , Peptidoglicano/biossíntese , Peptidoglicano/química , Peptidoglicano/metabolismoRESUMO
New, post-genomic analyses are increasing the rate at which information about highly complex processes such as bacterial growth and development can be acquired. The recent use of DNA-microarray and proteomic analysis to study the differentiating bacterium Caulobacter crescentus has provided the first global view of the requirements of a bacterium as it progresses through its cell cycle. Potential regulators of cell cycle progression have been identified, and it has been suggested that proteolysis could have a global role in regulating the bacterial cell cycle.
Assuntos
Caulobacter crescentus/citologia , Caulobacter crescentus/metabolismo , Ciclo Celular , Genes cdc , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Caulobacter crescentus/genética , Divisão Celular/genética , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos/genética , Análise de Sequência com Séries de Oligonucleotídeos , ProteomaRESUMO
Two recent reports describe mRNA and protein expression patterns in the bacterium Caulobacter crescentus. The combined use of DNA microarray and proteomic analyses provides a powerful new perspective for unraveling the global regulatory networks of this complex bacterium.
Assuntos
Caulobacter crescentus/genética , Ciclo Celular/genética , Divisão Celular/genética , Replicação do DNA/genética , Regulação Bacteriana da Expressão Gênica , Modelos BiológicosRESUMO
FtsZ is required throughout the cell division process in eubacteria and in archaea. We report the isolation of novel mutants of the FtsZ gene in Caulobacter crescentus. Clusters of charged amino acids were changed to alanine to minimize mutations that affect protein folding. Molecular modelling indicated that all the clustered-charged-to-alanine mutations had altered amino acids at the surface of the protein. Of 13 such mutants, four were recessive-lethal, three were dominant-lethal, and six had no discernible phenotype. An FtsZ depletion strain of Caulobacter was constructed to analyse the phenotype of the recessive-lethal mutations and used to show that they blocked cell division at distinct stages. One mutation blocked the initiation of cell division, two mutations blocked cell division randomly, and one mutation blocked both early and late stages of cell division. The effect of the recessive mutations on the subcellular localization of FtsZ was determined. Models to explain the various mutant phenotypes are discussed. This is the first set of recessive alleles of ftsZ blocked at different stages of cell division.
Assuntos
Proteínas de Bactérias/genética , Caulobacter/genética , Caulobacter/fisiologia , Divisão Celular , Proteínas do Citoesqueleto , Mutação , Sequência de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Genes Bacterianos , Genes Recessivos , Modelos Moleculares , Dados de Sequência Molecular , Conformação Proteica , Alinhamento de SequênciaRESUMO
Swarmer cells of Caulobacter crescentus are devoid of the cell division initiation protein FtsZ and do not replicate DNA. FtsZ is synthesized during the differentiation of swarmer cells into replicating stalked cells. We show that FtsZ first localizes at the incipient stalked pole in differentiating swarmer cells. FtsZ subsequently localizes at the mid-cell early in the cell cycle. In an effort to understand whether Z-ring formation and cell constriction are driven solely by the cell cycle-regulated increase in FtsZ concentration, FtsZ was artificially expressed in swarmer cells at a level equivalent to that found in predivisional cells. Immunofluorescence microscopy showed that, in these swarmer cells, simply increasing FtsZ concentration was not sufficient for Z-ring formation; Z-ring formation took place only in stalked cells. Expression of FtsZ in swarmer cells did not alter the timing of cell constriction initiation during the cell cycle but, instead, caused additional constrictions and a delay in cell separation. These additional constrictions were confined to sites close to the original mid-cell constriction. These results suggest that the timing and placement of Z-rings is tightly coupled to an early cell cycle event and that cell constriction is not solely dependent on a threshold level of FtsZ.
Assuntos
Proteínas de Bactérias/metabolismo , Caulobacter crescentus/metabolismo , Proteínas do Citoesqueleto , Proteínas de Bactérias/biossíntese , Caulobacter crescentus/citologia , Caulobacter crescentus/crescimento & desenvolvimento , Ciclo Celular , Divisão Celular , Fatores de TempoRESUMO
During the Caulobacter life cycle, the timing of DNA replication, cell division and development is precisely coordinated. Recent work has begun to unravel the complex regulatory networks that couple these processes. A key aspect of these regulatory networks is the dynamic localization of multiple histidine protein kinases that control a master response regulator, thus driving downstream pathways.
Assuntos
Caulobacter/crescimento & desenvolvimento , Ciclo Celular/fisiologia , Proteínas de Bactérias/metabolismo , Caulobacter/citologia , Divisão Celular/fisiologia , Replicação do DNA/fisiologia , Histidina Quinase , Proteínas Quinases/metabolismo , Transdução de SinaisRESUMO
Coordination of DNA replication and cell division is essential in order to ensure that progeny cells inherit a full copy of the genome. Caulobacter crescentus divides asymmetrically to produce a non-replicating swarmer cell and a replicating stalked cell. The global response regulator CtrA coordinates DNA replication and cell division by repressing replication initiation and transcription of the early cell division gene ftsZ in swarmer cells. We show that CtrA also mediates a DNA replication checkpoint of cell division by regulating the late cell division genes ftsQ and ftsA. CtrA activates transcription of the P(QA) promoter that co-transcribes ftsQA, thus regulating the ordered expression of early and late cell division proteins. Cells inhibited for DNA replication are unable to complete cell division. We show that CtrA is not synthesized in pre-divisional cells in which replication has been inhibited, preventing the transcription of P(QA) and cell division. Replication inhibition prevents the activation of the ctrA P2 promoter, which normally depends on CtrA phosphorylation. This suggests the possibility that CtrA phosphorylation may be affected by replication inhibition.
Assuntos
Proteínas de Bactérias/fisiologia , Caulobacter crescentus/citologia , Divisão Celular/fisiologia , Replicação do DNA/fisiologia , Proteínas de Ligação a DNA , Fatores de Transcrição , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Sequência de Bases , Caulobacter crescentus/genética , DNA Bacteriano , Genes Bacterianos , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Transcrição GênicaRESUMO
In Caulobacter crescentus, stalk biosynthesis is regulated by cell cycle cues and by extracellular phosphate concentration. Phosphate-starved cells undergo dramatic stalk elongation to produce stalks as much as 30 times as long as those of cells growing in phosphate-rich medium. To identify genes involved in the control of stalk elongation, transposon mutants were isolated that exhibited a long-stalk phenotype irrespective of extracellular phosphate concentration. The disrupted genes were identified as homologues of the high-affinity phosphate transport genes pstSCAB of Escherichia coli. In E. coli, pst mutants have a constitutively expressed phosphate (Pho) regulon. To determine if stalk elongation is regulated by the Pho regulon, the Caulobacter phoB gene that encodes the transcriptional activator of the Pho regulon was cloned and mutated. While phoB was not required for stalk synthesis or for the cell cycle timing of stalk synthesis initiation, it was required for stalk elongation in response to phosphate starvation. Both pstS and phoB mutants were deficient in phosphate transport. When a phoB mutant was grown with limiting phosphate concentrations, stalks only increased in length by an average of 1.4-fold compared to the average 9-fold increase in stalk length of wild-type cells grown in the same medium. Thus, the phenotypes of phoB and pst mutants were opposite. phoB mutants were unable to elongate stalks during phosphate starvation, whereas pst mutants made long stalks in both high- and low-phosphate media. Analysis of double pst phoB mutants indicated that the long-stalk phenotype of pst mutants was dependent on phoB. In addition, analysis of a pstS-lacZ transcriptional fusion showed that pstS transcription is dependent on phoB. These results suggest that the signal transduction pathway that stimulates stalk elongation in response to phosphate starvation is mediated by the Pst proteins and the response regulator PhoB.
Assuntos
Caulobacter/crescimento & desenvolvimento , Organofosfatos/metabolismo , Regulon/fisiologia , Sequência de Aminoácidos , Proteínas de Bactérias/fisiologia , Sequência de Bases , Caulobacter/genética , Análise Mutacional de DNA , DNA Bacteriano/química , Proteínas de Ligação a DNA/fisiologia , Dados de Sequência Molecular , Fatores de Transcrição/fisiologiaRESUMO
Attachment to surfaces by the prosthecate bacterium Caulobacter crescentus is mediated by an adhesive organelle, the holdfast, found at the tip of the stalk. Indirect evidence suggested that the holdfast first appears at the swarmer pole of the predivisional cell. We used fluorescently labeled lectin and transmission electron microscopy to detect the holdfast in different cell types. While the holdfast was readily detectable in stalked cells and at the stalked poles of predivisional cells, we were unable to detect the holdfast in swarmer cells or at the flagellated poles of predivisional cells. This suggests that exposure of the holdfast to the outside of the cell occurs during the differentiation of swarmer to stalked cells. To investigate the timing of holdfast synthesis and exposure to the outside of the cell, we have examined the regulation of a holdfast attachment gene, hfaA. The hfaA gene is part of a cluster of four genes (hfaABDC), identified in strain CB2A and involved in attachment of the holdfast to the polar region of the cell. We have identified the hfaA gene in the synchronizable C. crescentus strain CB15. The sequence of the CB2A hfaA promoter suggested that it was regulated by sigma54. We show that the transcription of hfaA from either strain is not dependent on sigma54. Using a hfaA-lacZ fusion, we show that the transcription of hfaA is temporally regulated during the cell cycle, with maximal expression in late-predivisional cells. This increase in expression is largely due to the preferential transcription of hfaA in the swarmer pole of the predivisional cell.
Assuntos
Adesinas Bacterianas/biossíntese , Aderência Bacteriana/genética , Caulobacter crescentus/genética , Proteínas de Ligação a DNA , Genes Bacterianos , Adesinas Bacterianas/genética , Caulobacter crescentus/crescimento & desenvolvimento , Caulobacter crescentus/ultraestrutura , Compartimento Celular , Ciclo Celular , Clonagem Molecular , RNA Polimerases Dirigidas por DNA/metabolismo , Regulação Bacteriana da Expressão Gênica , Dados de Sequência Molecular , RNA Polimerase Sigma 54 , Análise de Sequência de DNA , Fator sigma/metabolismo , Transcrição GênicaRESUMO
During exponential growth, each cell cycle of the alpha-purple bacterium Caulobacter crescentus gives rise to two different cell types: a motile swarmer cell and a sessile stalked cell. When cultures of C. crescentus are grown for extended periods in complex (PYE) medium, cells undergo dramatic morphological changes and display increased resistance to stress. After cultures enter stationary phase, most cells are arrested at the predivisional stage. For the first 6-8 days after inoculation, the colony-forming units (cfu) steadily decrease from 10(9) cfu ml(-1) to a minimum of 3x10(7) cfu ml(-1) after which cells gradually adopt an elongated helical morphology. For days 9-12, the cfu of the culture increase and stabilize around 2 x 10(8) cfu ml(-1). The viable cells have an elongated helical morphology with no constrictions and an average length of 20 microm, which is 15-20 times longer than exponentially growing cells. The level of the cell division initiation protein FtsZ decreases during the first week in stationary phase and remains at a low constant level consistent with the lack of cell division. When resuspended in fresh medium, the elongated cells return to normal size and morphology within 12 h. Cells that have returned from stationary phase proceed through the same developmental changes when they are again grown for an extended period and have not acquired a heritable growth advantage in stationary phase (GASP) compared with overnight cultures. We conclude that the changes observed in prolonged cultures are the result of entry into a new developmental pathway and are not due to mutation.
Assuntos
Caulobacter crescentus/citologia , Proteínas do Citoesqueleto , Proteínas de Bactérias/metabolismo , Caulobacter crescentus/crescimento & desenvolvimento , Caulobacter crescentus/metabolismo , Divisão Celular/efeitos dos fármacos , Contagem de Colônia Microbiana , Meios de Cultura , Temperatura Alta , Peróxido de Hidrogênio/farmacologia , Concentração de Íons de Hidrogênio , Imuno-Histoquímica , Interfase/efeitos dos fármacos , Proteínas de Membrana/metabolismo , Proteínas Quimiotáticas Aceptoras de Metil , Microscopia Eletrônica , Fenótipo , Fatores de TempoRESUMO
Stalk synthesis in Caulobacter crescentus is a developmentally controlled and spatially restricted event that requires the synthesis of peptidoglycan at the stalk-cell body junction. We show that the beta-lactam antibiotic mecillinam prevents stalk synthesis by inhibiting stalk elongation. In addition, mecillinam causes an increase in the diameter of the stalk at the stalk-cell body junction. We describe two mutations that confer resistance to mecillinam and that prevent stalk elongation. These mutations are probably allelic, and they map to a locus previously not associated with stalk synthesis.
Assuntos
Andinocilina/farmacologia , Caulobacter crescentus/efeitos dos fármacos , Resistência às Penicilinas/genética , Penicilinas/farmacologia , Caulobacter crescentus/genética , Caulobacter crescentus/crescimento & desenvolvimento , Mutação , FenótipoRESUMO
The mechanisms by which bacterial cell division and DNA replication are co-ordinated are still unknown. We have used the easily synchronizable bacterium Caulobacter crescentus to determine when the cell division genes ftsQ and ftsA are transcribed during the DNA replication cycle and to compare their transcription with that of ftsZ. Unlike the situation in Escherichia coli, transcription of ftsQ and ftsA does not extend into ftsZ in Caulobacter. ftsQ and ftsA are co-transcribed by a strong promoter, P(QA), present within the end of the ddl gene upstream of ftsQ. Transcription of P(QA) is turned on at the end of the DNA replication period, coincident with the end of the ftsZ transcription period. ftsA is also transcribed by another promoter, P(A), present between ftsQ and ftsA. P(A) transcription is approximately 10 times weaker than P(QA) and occurs during the DNA replication period. Transcription of ftsA by P(A) is sufficient for cell viability, but is not sufficient for normal cell division. When the transcription of ftsA is increased constitutively, cell division is inhibited and stalks are synthesized at aberrant positions. Thus, transcription of ftsA and ftsZ mimics their order of action in Caulobacter and proper transcription of ftsA has to be maintained for normal cell division and differentiation.
Assuntos
Proteínas de Bactérias/genética , Caulobacter crescentus/crescimento & desenvolvimento , Caulobacter crescentus/genética , Ciclo Celular , Proteínas do Citoesqueleto , Proteínas de Escherichia coli , Proteínas de Membrana/genética , Sequência de Aminoácidos , Sequência de Bases , Caulobacter crescentus/ultraestrutura , Divisão Celular , Replicação do DNA , Expressão Gênica , Microscopia Eletrônica , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Transcrição GênicaRESUMO
The cell division protein FtsZ is composed of three regions based on sequence similarity: a highly conserved N-terminal region of approximately 320 amino acids; a variable spacer region; and a conserved C-terminal region of eight amino acids. We show that FtsZ mutants missing different C-terminal fragments have dominant lethal effects because they block cell division in Caulobacter crescentus by two different mechanisms. Removal of the C-terminal conserved region, the linker, and 40 amino acids from the end of the N-terminal conserved region (FtsZdeltaC281) prevents the localization or the polymerization of FtsZ. Because two-hybrid analysis indicates that FtsZdeltaC281 does not interact with FtsZ, we hypothesize that FtsZdeltaC281 blocks cell division by competing with a factor required for FtsZ localization or that it titrates a factor required for the stability of the FtsZ ring. The removal of 24 amino acids from the C-terminus of FtsZ (FtsZdeltaC485) causes a punctate pattern of FtsZ localization and affects its interaction with FtsA. This suggests that the conserved C-terminal region of FtsZ is required for proper polymerization of FtsZ in Caulobacter and for its interaction with FtsA.
Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Caulobacter crescentus/genética , Proteínas do Citoesqueleto , Sequência de Aminoácidos , Proteínas de Bactérias/química , Caulobacter crescentus/crescimento & desenvolvimento , Caulobacter crescentus/metabolismo , Ciclo Celular , Divisão Celular , Sequência Conservada , Imunofluorescência , Regulação Bacteriana da Expressão Gênica , Técnicas Genéticas , Immunoblotting , Fenótipo , Deleção de SequênciaRESUMO
In the differentiating bacterium Caulobacter crescentus, the cell division initiation protein FtsZ is present in only one of the two cell types. Stalked cells initiate a new round of DNA replication immediately after cell division and contain FtsZ, whereas the progeny swarmer cells are unable to initiate DNA replication and do not contain FtsZ. We show that FtsZ expression is controlled by cell cycle-dependent transcription and proteolysis. Transcription of ftsZ is repressed in swarmer cells and is activated concurrently with the initiation of DNA replication. At the end of the DNA replication period, transcription of ftsZ decreases substantially. We show that the global cell cycle regulator CtrA is involved in the cell cycle control of ftsZ transcription. CtrA binds to a site that overlaps the ftsZ transcription start site. Removal of the CtrA-binding site results in transcription of the ftsZ promoter in swarmer cells. Decreasing the cellular concentration of CtrA increases ftsZ transcription and conversely, increasing the concentration of CtrA decreases ftsZ transcription. Because CtrA is present in swarmer cells, is degraded at the same time as ftsZ transcription begins, and reappears when ftsZ transcription decreases at the end of the cell cycle, we propose that CtrA is a repressor of ftsZ transcription. We show that proteolysis is an important determinant of cell type-specific distribution and cell cycle variation of FtsZ. FtsZ is stable when it is synthesized and assembles into the cytokinetic ring at the beginning of the cell cycle. After the initiation of cell division, the rate of FtsZ degradation increases as both the constriction site and the FtsZ ring decrease in diameter. When ftsZ is expressed constitutively from inducible promoters, the abundance of FtsZ still varies during the cell cycle. The coupling of transcription and proteolysis to cell division ensures that FtsZ is inherited only by the progeny cell that will begin DNA replication immediately after cell division.
Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/fisiologia , Caulobacter crescentus/genética , Caulobacter crescentus/fisiologia , Ciclo Celular/fisiologia , Proteínas do Citoesqueleto , Proteínas de Ligação a DNA , Fatores de Transcrição , Sequência de Bases , Sítios de Ligação/genética , Caulobacter crescentus/química , Ciclo Celular/genética , Divisão Celular/genética , Divisão Celular/fisiologia , Proteínas de Ligação ao GTP/genética , Proteínas de Ligação ao GTP/fisiologia , Regulação Bacteriana da Expressão Gênica , Hidrólise , Dados de Sequência Molecular , Mutação/genética , Mutação/fisiologia , Regiões Promotoras Genéticas/genética , Processamento de Proteína Pós-Traducional , Homologia de Sequência do Ácido Nucleico , Fatores de Tempo , Transcrição Gênica/genética , Transcrição Gênica/fisiologiaRESUMO
The alternative sigma factor sigma54 is required for the biogenesis of both the flagellum and the stalk in Caulobacter crescentus. The DNA sequence downstream of the sigma54 gene (rpoN) has been determined, revealing three open reading frames (ORFs) encoding peptides of 203, 208, and 159 amino acids. ORF208 and ORF159 are homologous to ORFs found downstream of rpoN in other microorganisms. The organization of this region in C. crescentus is similar to that in other bacteria, with the exception of an additional ORF, ORF203, immediately downstream from rpoN. There is a single temporally regulated promoter that drives the expression of both rpoN and ORF203. Promoter probe analysis indicates the presence of another promoter downstream from ORF203 which exhibits a temporal control that is different from that of the rpoN promoter. Mutational analysis was used to address the function of the proteins encoded by these three downstream ORFs. The mutations have no effect on the transcription of previously known sigma54-dependent flagellar promoters except for a slight effect of an ORF159 mutation on transcription of fljK.
Assuntos
Caulobacter crescentus/genética , Proteínas de Ligação a DNA , RNA Polimerases Dirigidas por DNA/genética , Óperon , Fator sigma/genética , Transcrição Gênica , Sequência de Aminoácidos , Sequência de Bases , Caulobacter crescentus/crescimento & desenvolvimento , Ciclo Celular , Análise Mutacional de DNA , RNA Polimerases Dirigidas por DNA/química , Flagelos/genética , Flagelos/metabolismo , Dados de Sequência Molecular , Mutagênese Insercional , Fases de Leitura Aberta , Fenótipo , Regiões Promotoras Genéticas , RNA Polimerase Sigma 54 , Alinhamento de Sequência , Análise de Sequência de DNA , Fator sigma/químicaRESUMO
Many genes involved in cell division and DNA replication and their protein products have been identified in bacteria; however, little is known about the cell cycle regulation of the intracellular concentration of these proteins. It has been shown that the level of the tubulin-like GTPase FtsZ is critical for the initiation of cell division in bacteria. We show that the concentration of FtsZ varies dramatically during the cell cycle of Caulobacter crescentus. Caulobacter produce two different cell types at each cell division: (i) a sessile stalked cell that can initiate DNA replication immediately after cell division and (ii) a motile swarmer cell in which DNA replication is blocked. After cell division, only the stalked cell contains FtsZ. FtsZ is synthesized slightly before the swarmer cells differentiate into stalked cells and the intracellular concentration of FtsZ is maximal at the beginning of cell division. Late in the cell cycle, after the completion of chromosome replication, the level of FtsZ decreases dramatically. This decrease is probably mostly due to the degradation of FtsZ in the swarmer compartment of the predivisional cell. Thus, the variation of FtsZ concentration parallels the pattern of DNA synthesis. Constitutive expression of FtsZ leads to defects in stalk biosynthesis suggesting a role for FtsZ in this developmental process in addition to its role in cell division.
Assuntos
Proteínas de Bactérias/metabolismo , Caulobacter/citologia , Proteínas do Citoesqueleto , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Caulobacter/metabolismo , Ciclo Celular , Clonagem Molecular , Escherichia coli/genética , Dados de Sequência Molecular , Homologia de Sequência de AminoácidosAssuntos
Caulobacter/citologia , Regulação Bacteriana da Expressão Gênica/fisiologia , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Proteínas de Bactérias/análise , Caulobacter/genética , Caulobacter/metabolismo , Caulobacter/ultraestrutura , Replicação do DNA , DNA Bacteriano/metabolismo , Metilação , Organelas/ultraestruturaRESUMO
The transcription of many spatially and temporally controlled flagellar structural genes in Caulobacter requires the RNA polymerase sigma 54 subunit. Like flagellar biogenesis, stalk formation is an asymmetric polar morphogenesis that occurs once each cell cycle in response to internal cell cycle signals. We have isolated the sigma 54 gene (rpoN) and describe here a novel role for this alternative sigma-factor in cell differentiation: It is required for the biogenesis of both polar structures, and the disruption of the rpoN gene results in aberrant cell division. Surprisingly, the transcription of rpoN is temporally regulated during the cell cycle; it increases 10-fold commensurate with stalk formation and just before the onset of flagellar gene expression. These results suggest that sigma 54 abundance responds to cell cycle cues and is involved in the global timing of the central events of Caulobacter development, whereas the transcriptional activators of sigma 54-dependent promoters are responsible for the refined control of the expression of individual or small groups of genes required for each specific event.
Assuntos
Caulobacter/crescimento & desenvolvimento , Proteínas de Ligação a DNA , RNA Polimerases Dirigidas por DNA , Fator sigma/fisiologia , Sequência de Aminoácidos , Sequência de Bases , Caulobacter/genética , Divisão Celular/genética , DNA Bacteriano , Flagelos , Regulação Bacteriana da Expressão Gênica , Teste de Complementação Genética , Dados de Sequência Molecular , Morfogênese/genética , Mutação , Nitrogênio/metabolismo , Fenótipo , Fosfatos/metabolismo , RNA Polimerase Sigma 54 , Homologia de Sequência de Aminoácidos , Fator sigma/genéticaRESUMO
We have developed a simple rapid plasmid DNA mini-preparation method which yields DNA of sufficient quality to be used in large scale sequencing projects. The method, which is a modification of the alkaline method of Birnboim and Doly (1979), requires less than two hours. We have eliminated the use of organic extractions, RNase digestion and alkaline denaturation of the DNA for annealing of the primer. The proportion of supercoiled plasmid DNA obtained is close to 100%. Greater than 80% of the clones yield at least 500 bp of sequence information per primer. The sequencing reactions from these double-stranded templates can be done on both strands using the universal and reverse sequence primers with the usual two reactions per primer, one to read close to the primer and one to read far from it. Thus, each clone yields at least 1 kb of sequence information. The preparation of the templates and the sequencing reactions can be done in less than three hours so that the sequencing gel can be run the same day.