RESUMO
The aim of this study was to investigate the development of microcells in the human sarcoma cell line HT-1080 after interference with thiophosphamidum. We found that damaged interphase macrocells located at the projection of the nucleolus may form one or several microcells. The micronuclei of the microcells intensively incorporate the thymidine analogue 5-bromo-2'-deoxyuridine and strongly express argyrophilic nucleolar organiser region proteins. At an early phase of the development, the micronuclei contain fragmented DNA, but in subsequent phases, the micronuclei accumulate polymeric DNA, simultaneously with an increase in their size. After desintegration of the damaged macrocell, the microcells appear in the intercellular space. The microcells can enter mitosis and they strongly express the lung resistance protein. Electron microscopic observations suggest that coiled bodies are involved in the development of the microcells. Since the observed path of microcell formation differs from apoptotic cell fragmentation into apoptotic bodies, we propose a new term for this microcell development: sporosis. We suggest that self-renewal of the tumour stem cells is likely based on sporosis.
Assuntos
Sarcoma/patologia , Bromodesoxiuridina/metabolismo , Núcleo Celular/química , Núcleo Celular/patologia , Núcleo Celular/ultraestrutura , DNA de Neoplasias/metabolismo , Humanos , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Micronúcleos com Defeito Cromossômico/química , Micronúcleos com Defeito Cromossômico/metabolismo , Micronúcleos com Defeito Cromossômico/patologia , Proteínas de Neoplasias/biossíntese , Região Organizadora do Nucléolo/química , Região Organizadora do Nucléolo/ultraestrutura , Sarcoma/metabolismo , Coloração pela Prata , Células Tumorais Cultivadas/citologia , Partículas de Ribonucleoproteínas em Forma de Abóbada/biossínteseRESUMO
There was found a correlation between dynamic changes in the serotonin level and mortality in rats with 9,10-dimethyl-1,2-benzanthracene induced blastomogenesis. An estimation was made of the influence of monoamine oxidase inhibitors, transamine and D, L-malic acid benzyldihydrazide on the type of a tumor produced. Chemical blastomogenesis in the case of cerebellar tumors is shown to be associated with the decreased serotonin content at the site of a tumor. But fluctuations of the serotonin level in the hypothalamus have not been detected. This evidenced that the decreased mortality rate caused by the administration of monoamine oxidase inhibitor is due to a lower production of astrocytomas and genetically related glioblastomas. The role of serotonin in increasing the astrocyte resistance to malignant degeneration has been discussed.
Assuntos
Aminas/metabolismo , Neoplasias Encefálicas/etiologia , 9,10-Dimetil-1,2-benzantraceno , Animais , Neoplasias Encefálicas/metabolismo , Neoplasias Cerebelares/etiologia , Neoplasias Cerebelares/metabolismo , Inibidores da Monoaminoxidase/farmacologia , Neoplasias Experimentais/etiologia , Neoplasias Experimentais/metabolismo , Ratos , Serotonina/metabolismo , Fatores de TempoRESUMO
A principal scheme of the digital scanning and intergrating cytospectrophotometer is described which permits to measure the integrating optical density of specifically stained preparations, "spot" optical density of any absorbing preparations and the area of absorbing objects in two different levels of absorbtion.