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It is well known that there is a high concentration of lactate in sweat. Interest in measuring sweat lactate has arisen from its potential role in several clinical and sport performance applications. However, the effect of heat acclimation on sweat lactate concentration is still under debate. This is partly because sweat lactate concentration is greatly affected by sweat rate, which is known to increase during heat acclimation. Thus, to better understand this issue it is necessary to account for sweat rate - which has not been done previously in the literature. Therefore, the purpose of the current study was to determine the effect of heat acclimation on the relationship between sweat rate vs. sweat lactate concentration. Six subjects completed a 7-day heat acclimation protocol. The daily 2-h exercise bout was split into three 40-min intervals with exercise intensity increasing with each successive interval. This was done to induce three different sweat rates to determine the sweat rate vs. sweat lactate concentration relationship before and after heat acclimation for each participant. A 2 (heat acclimation) x 3 (sweat rate) repeated measures ANOVA was conducted to determine statistical significance. There was a significant (p < 0.05) decrease in the grand mean sweat lactate concentration over the course of seven days of heat acclimation from 17.0 ± 5.0 to 11.3 ± 1.1 mmol/L (p < 0.05). A significant (p < 0.05) heat acclimation x sweat rate ordinal interaction was also found. The results of the current study show that heat acclimation significantly decreases the sweat lactate concentration. In addition, there was a significant ordinal interaction which suggests that the impact of sweat rate on sweat lactate concentration is decreased following heat acclimation.
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Temperatura Alta , Suor , Aclimatação , Humanos , Ácido Láctico , SudoreseRESUMO
Background: To investigate how rapid changes in hydration affect urine color expressed in CIE L*a*b* colorspace. Methods: This study was a two-day crossover design where subjects (N = 30) came in one visit dehydrated, after a 15 h overnight fluid deprivation, and rapidly rehydrated by drinking at least 1000 mL of water in 2 h. On the other visit subjects reported euhydrated and then rapidly dehydrated 2% by walking (3 mph) in a heat chamber (100°F, 50% humidity) for 2 h. Urine samples on both days were collected pre- and post-dehydration/rehydration. Urine osmolality, urine specific gravity, subjective urine color and objective urine color expressed in CIE L*a*b* colorspace were measured. Results: In the dehydration trial participants experienced a significant weight loss of approximately 2% of their starting, euhydrated body weight. The CIE urine color L*-value significantly decreased (-2.3 units) while the b*-value significantly increased (16 units). Subjective urine color significantly increased (1 unit). Urine osmolality increased (25 mmol/kg) and urine specific gravity increased (0.002 g/mL) between the pre- and post-dehydration conditions, however, neither of these changes were statistically significant. In the rehydration trial participants had a significant 1.5% increase in body weight after the ingestion of water. Significant increases were observed in the CIE urine color L*-value (7 units) and a*-value (1.1 units), while the b*-value significantly decreased (-24 units). Subjective urine color significantly decreased (-3 units). Urine osmolality (-600 mmol/kg) and urine specific gravity (-0.018 g/mL) significantly decreased between the pre- and post-rehydration conditions. Conclusions: Traditional markers of hydration, including urine osmolality and urine specific gravity, did not significantly change in the acute dehydration trial, suggesting that these values may not be responsive to rapid changes in hydration status. However, the CIE L*- and b*-values of urine color significantly decreased in the rapid dehydration trial and significantly increased in the rapid rehydration trial. Thus, the results of the current study suggest that urine color L*- and b*-values expressed in the CIE L*a*b* colorspace were more responsive to changes in hydration status during rapid dehydration than traditional indices of urine concentration and thus may be better markers under such conditions.
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BACKGROUND: The purpose of this study was to explore the relationship between concentrations of amino acid (AA) and related metabolites in plasma and sweat obtained before and after exercise performed at different intensities and therefore different rates of sweat loss. METHODS: Ten subjects completed a maximally ramped exercise test and three 30-min submaximal (45/60/75% VO
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Aminoácidos , Suor , Estudos Cross-Over , Exercício Físico , Humanos , Suor/metabolismo , SudoreseRESUMO
Urine color has been shown to be a viable marker of hydration status in healthy adults. Traditionally, urine color has been measured using a subjective color scale. In recent years, tristimulus colorimetry developed by the International Commission on Illumination (CIE L*a*b*) has been widely adopted as the reference method for color analysis. In the L*a*b* color space, L* indicates lightness ranging from 100 (white) to 0 (black), while a* and b* indicate chromaticity. a* and b* are color directions: -a* is the green axis, +a* is the red axis, -b* is the blue axis, and +b* is the yellow axis. The L*a*b* color space model is only accurately represented in three-dimensional space. Considering the above, the purpose of the current study was to evaluate urine color during different hydration states, with the results expressed in CIE L*a*b* color space. The study included 28 healthy participants (22 males and 6 females) ranging between the age of 20 and 67 years (28.6 ± 11.3 years). One hundred and fifty-one urine samples were collected from the subjects in various stages of hydration, including morning samples after 7-15 h of water deprivation. Osmolality and CIE L*a*b* parameters were measured in each sample. As the urine osmolality increased, a significant linear increase in b* values was observed as the samples became more pronouncedly yellow (τb = 0.708). An increase in dehydration resulted in darker and significantly more yellow urine, as L* values decreased in lightness and b* values increased along the blue-yellow axis. However, as dehydration increased, a notable polynomial trend in color along the green-red axis was observed as a* values initially decreased, indicating a green hue in slightly dehydrated urine, and then increased as urine became more concentrated and thus more dehydrated. It was determined that 74% of the variance seen in urine osmolality was due to CIE L*a*b* variables. This newfound knowledge about urine color change along with the presented regression model for predicting urine osmolality provides a more detailed and objective perspective on the effect of hydration on urine color, which to our knowledge has not been previously researched.
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INTRODUCTION: Prolonged cold-water immersion (CWI) has the potential to cause significant hypothermia and haemoconcentration; both of which have previously been shown to independently increase blood viscosity in vitro. The purpose of this study was to determine the effect of CWI on blood viscosity and examine the relative contribution of decreased blood temperature and haemoconcentration. METHODS: Ten healthy volunteers were immersed to mid-sternum in 10°C water for 90 minutes. Gastrointestinal (GI) temperature, haematocrit (Hct), and blood viscosity were measured pre- and post-CWI. RESULTS: CWI caused mean (SD) GI temperature to decrease from 37.5 (0.3)°C to 36.2 (0.7)°C (P < 0.05). CWI also caused mean Hct to increase from 40.0 (3.5)% to 45.0 (2.9)% (P < 0.05). As a result of the haemoconcentration and decreased GI temperature during CWI the mean blood viscosity increased by 19% from 2.80 (0.28) mPa·s⻹ to 3.33 (0.42) mPa·s⻹ (P < 0.05). However, when the pre-CWI blood sample was measured at the post-CWI GI temperature (36.2°C) there was no significant difference in the blood viscosity when compared to the pre-CWI (37.5°C) blood sample (2.82 (0.20) mPa·s-1 and 2.80 (0.28) mPa·s-1 respectively). Furthermore, the changes in Hct and blood viscosity during CWI were significantly correlated with an r = 0.84. CONCLUSION: The results of the current study show that prolonged, severe CWI causes a significant 19% increase in blood viscosity. In addition, the results strongly suggest that almost all of the increased blood viscosity seen following CWI is the result of haemoconcentration, not decreased blood temperature.
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Temperatura Corporal , Hipotermia , Viscosidade Sanguínea , Exercício Físico , Feminino , Humanos , Imersão , Masculino , Temperatura , ÁguaRESUMO
OBJECTIVES: Examine effect of single hand heating with and without negative pressure on fasting blood glucose (FBG) and postprandial blood glucose (PBG). DESIGN: Double-blind randomized controlled trial with crossover design. SUBJECTS: FBG experiment: 17 healthy subjects (4 males). PBG experiment: 13 healthy subjects (1 males). INTERVENTIONS: Devices included one providing heat only, one heat and negative pressure, and one acting as a sham. For the FBG experiment the devices were used for 30â¯min. For the PBG experiment the devices were used for one hour during an oral glucose tolerance test (OGTT). OUTCOME MEASURES: Blood glucose measurements were used to determine change in FBG, peak PBG, area under the curve (AUC), and incremental AUC (iAUC). RESULTS: Temperature: Change in tympanic temperature was ≤ 0.15⯰C for all trials. FBG: There was no effect on FBG. PBG: Compared to the sham device the heat plus vacuum and heat only device lowered peak blood glucose by 16(31)mg/dL, pâ¯=â¯0.092 and 18(28)mg/dL, pâ¯=â¯0.039, respectively. AUC and iAUC: Compared to the sham device, the heat plus vacuum device and heat only device lowered the AUC by 5.1(15.0)%, pâ¯=â¯0.234 and 7.9(11.1)%, pâ¯=â¯0.024 respectively and iAUC by 17.2(43.4)%, pâ¯=â¯0.178 and 20.5(34.5)%, pâ¯=â¯0.054, respectively. CONCLUSIONS: Heating a single hand lowers postprandial blood glucose in healthy subjects.
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Glicemia/análise , Mãos/fisiologia , Temperatura Alta/uso terapêutico , Período Pós-Prandial , Adolescente , Adulto , Estudos Cross-Over , Método Duplo-Cego , Feminino , Voluntários Saudáveis , Humanos , Masculino , Adulto JovemRESUMO
The purpose of this study was to determine the cardiovascular, thermoregulatory, and perceived exertion responses during 2â¯h of moderate intensity exercise in a combined high heat (38⯰C, 40% relative humidity) and hypoxic (15% O2) environment. Ten healthy volunteers completed 2â¯h of treadmill walking at 40% of maximal oxygen uptake in four different conditions, each separated by approximately 1 week: (1) control, 23⯰C/20.9% O2, (2) heat, 38⯰C/20.9% O2, (3) hypoxia, 23⯰C/15% O2, and (4) combined heat/hypoxia, 38⯰C/15% O2. Compared to the responses seen in each condition alone, heart rate (HR) and core temperature (Tcore) showed an additive increase in the combined heat and hypoxic environment after 2â¯h of moderate intensity exercise. The most important new finding was that the mean rating of perceived exertion (RPE) increased synergistically 3.3 units when exercising in the combined high heat and hypoxic environment, compared to 1.9 units in the heat condition alone. The results suggest that RPE is a conscious perception of effort that plays a regulatory function to ensure that the work rate remains at an intensity that can be safely sustained, rather than simply a marker of exercise intensity. Such results also support previous anecdotal reports that exercise on hot days at altitude seem unusually difficult.
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Exercício Físico/psicologia , Esforço Físico , Adulto , Altitude , Temperatura Corporal , Exercício Físico/fisiologia , Feminino , Frequência Cardíaca , Temperatura Alta , Humanos , Hipóxia , Masculino , Percepção , Adulto JovemRESUMO
Exposure to cold causes disturbances in cognitive performance that can have a profound impact on the safety, performance, and success of populations that frequent cold environments. It has recently been suggested that repeated cold stress, resulting in cold acclimation, may be a potential strategy to mitigate the cognitive impairments frequently seen upon exposure to cold temperatures. The purpose of this study, therefore, was to examine cognitive and neurophysiological function during repeated cold water immersion. Twelve healthy participants consisting of 8 males and 4 females (mean⯱â¯SD age: 26⯱â¯5â¯years, height: 174.0⯱â¯8.9â¯cm, weight: 75.6⯱â¯13.1â¯kg) completed seven 90-minute immersions in 10⯰C water, each separated by 24â¯h. During immersions 1, 4, and 7, a double-digit addition task and a computer-based psychomotor vigilance task (PVT) were administered to assess cognitive performance, while neurophysiological function was assessed using electroencephalography (EEG) measurements collected during the PVT. Findings suggest that participants experienced an insulative type of cold acclimation, evidenced by greater heat retention and less shivering, with possible improvements in cognitive performance. Participants had more correct responses on the double-digit addition task on Immersion 7 (39⯱â¯5) compared with Immersion 1 (33⯱â¯6); pâ¯<â¯0.001, yet no differences were observed for reaction time between Immersion 7 (286⯱â¯31â¯ms) and Immersion 1 (281⯱â¯19â¯ms); pâ¯=â¯0.59. Additionally, EEG analyses indicate no beneficial changes in neurophysiological function. Results demonstrate that individuals who are frequently exposed to cold water may be more suited to handle certain cognitive challenges after several exposures, although additional investigations are needed to provide neurophysiological support for this.
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Aclimatação/fisiologia , Cognição/fisiologia , Temperatura Baixa/efeitos adversos , Adulto , Eletroencefalografia/métodos , Feminino , Voluntários Saudáveis , Humanos , Masculino , Estresse Fisiológico/fisiologia , ÁguaRESUMO
BACKGROUND: The purpose of this study was to measure sweat rate during exercise in the heat after directly inhibiting carbonic anhydrase (CA) in eccrine sweat glands via transdermal iontophoresis of acetazolamide. It was hypothesized that if CA was important for sweat production, local administration of acetazolamide, without the confounding systemic effects of dehydration typically associated with past studies, would have a significant effect on sweat rate during exercise. METHODS: Ten healthy subjects volunteered to exercise in the heat following acetazolamide or distilled water iontophoresis on the forearm. RESULTS: The distilled water iontophoresis site had a mean sweat rate during exercise in the heat of 0.59±0.31 µL/cm2/min, while the acetazolamide iontophoresis site had a mean sweat rate of 0.63±0.36 µL/cm2/min (p>0.05). CONCLUSIONS: The most important finding of the current study was that iontophoresis of acetazolamide did not significantly decrease sweat rate during exercise in the heat. Such results suggest that in past studies it was systemic dehydration, and not CA inhibition at the level of the sweat gland, that caused the reported decreased sweat rate.
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Acetazolamida/administração & dosagem , Inibidores da Anidrase Carbônica/administração & dosagem , Exercício Físico/fisiologia , Iontoforese/métodos , Sudorese/efeitos dos fármacos , Acetazolamida/metabolismo , Adulto , Inibidores da Anidrase Carbônica/metabolismo , Anidrases Carbônicas/metabolismo , Glândulas Écrinas/efeitos dos fármacos , Glândulas Écrinas/enzimologia , Feminino , Temperatura Alta/efeitos adversos , Humanos , Masculino , Sudorese/fisiologiaRESUMO
PURPOSE: It is well-documented that severe cold stress impairs exercise performance. Repeated immersion in cold water induces an insulative type of cold acclimation, wherein enhanced vasoconstriction leads to greater body heat retention, which may attenuate cold-induced exercise impairments. The purpose of this study, therefore, was to investigate changes in exercise performance during a 7-day insulative type of cold acclimation. METHODS: Twelve healthy participants consisting of eight males and four females (mean ± SD age: 25.6 ± 5.2 years, height: 174.0 ± 8.9 cm, weight: 75.6 ± 13.1 kg) performed a 20 min self-paced cycling test in 23 °C, 40% humidity without prior cold exposure. Twenty-four hours later they began a 7-day cold acclimation protocol (daily 90 min immersion in 10 °C water). On days one, four, and seven of cold acclimation, participants completed the same cycling test. Measurements of work completed, core and skin temperatures, heart rate, skin blood flow, perceived exertion, and thermal sensation were measured during each cycling test. RESULTS: Successful insulative cold acclimation was observed. Work produced during the baseline cycling test (220 ± 70 kJ) was greater (p < 0.001) than all three tests that were performed following immersions (195 ± 58, 197 ± 60, and 194 ± 62 kJ) despite similar ratings of perceived exertion during each test, suggesting that cold exposure impaired cycling performance. This impairment, however, was not attenuated over the cold acclimation period. CONCLUSIONS: Results suggest that insulative cold acclimation does not attenuate impairments in exercise performance that were observed following acute cold water immersion.
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Aclimatação , Desempenho Atlético , Temperatura Baixa , Exercício Físico/fisiologia , Adulto , Feminino , Humanos , Imersão , Masculino , VasoconstriçãoRESUMO
The purpose of this study was to determine the time course for the previously reported reduction in sweat sodium ion concentration during heat acclimation. Four healthy volunteers completed 7 consecutive days of heat acclimation which included 2h of treadmill walking in a 40°C and 40% relative humidity environment. A modified constant hyperthermia protocol was used as workloads were increased each day to maintain a constant core temperature over the 7 days of heat acclimation. Forearm sweat was collected 3 times during each 2h exercise bout on days 1, 3, 5, and 7 of heat acclimation. Forearm sweat rate and sweat sodium ion concentration were determined from each sample. The results showed that there was a significant (p < 0.05) downward shift in the mean sweat rate vs. sweat sodium ion concentration relationship on days 3, 5, and 7 of heat acclimation, as compared to the pre-heat acclimation (day 1) data. Thus, at any given sweat rate, heat acclimation resulted in a significantly lower sweat sodium ion concentration. The response was very rapid and occurred following only 2 consecutive days of heat exposure (i.e., day 3 vs. day 1 data). Furthermore, the calculated sweat sodium ion concentration, at a sweat rate of 1µl/cm2/min, decreased linearly (r = - 0.50, p < 0.05) during the 7 days of heat acclimation. Such results suggest that heat acclimation rapidly improves sodium ion reabsorption from the eccrine sweat gland duct as evidenced by significant reductions in the sweat sodium ion concentration.
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Aclimatação , Temperatura Alta , Sódio/análise , Suor/química , Adulto , Feminino , Humanos , Masculino , SudoreseRESUMO
Athletes, occupational workers, and military personnel experience cold temperatures through cold air exposure or cold water immersion, both of which impair cognitive performance. Prior work has shown that neurophysiological pathways may be sensitive to the effects of temperature acclimation and, therefore, cold acclimation may be a potential strategy to attenuate cold-induced cognitive impairments for populations that are frequently exposed to cold environments. This review provides an overview of studies that examine repeated cold stress, cold acclimation, and measurements of cognitive performance to determine whether or not cold acclimation provides beneficial protection against cold-induced cognitive performance decrements. Studies included in this review assessed cognitive measures of reaction time, attention, logical reasoning, information processing, and memory. Repeated cold stress, with or without evidence of cold acclimation, appears to offer no added benefit of improving cognitive performance. However, research in this area is greatly lacking and, therefore, it is difficult to draw any definitive conclusions regarding the use of cold acclimation to improve cognitive performance during subsequent cold exposures. Given the current state of minimal knowledge on this topic, athletes, occupational workers, and military commands looking to specifically enhance cognitive performance in cold environments would likely not be advised to spend the time and effort required to become acclimated to cold. However, as more knowledge becomes available in this area, recommendations may change.
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Aclimatação , Cognição , Temperatura Baixa , Aclimatação/fisiologia , Cognição/fisiologia , Humanos , Estresse Fisiológico/fisiologiaRESUMO
INTRODUCTION: Warfighters often train and conduct operations in cold environments. Specifically, military trainees and divers that are repeatedly exposed to cold water may experience inadvertent cold acclimatization, which results in body heat retention. These same warfighters can quickly switch between environments (cold to hot or hot to cold) given the nature of their work. This may present a risk of early onset of hyperthermia when cold-acclimatized warfighters are subsequently exposed to physiological insults that increase body temperature, such as exercise and heat stress. However, there is currently no evidence that suggests this is the case. The purpose of this work, therefore, is to determine what impact, if any, repeated immersion in cold water has on subsequent exercise in the heat. MATERIALS AND METHODS: Twelve healthy subjects (values in mean ± SD: age, 25.6 ± 5.2 years; height, 174.0 ± 8.9 cm; weight, 75.6 ± 13.1 kg) voluntarily provided written informed consent in accordance with the San Diego State University Institutional Review Board. They first completed 120 minutes of moderate treadmill walking in 40°C and 40% relative humidity. During this trial, subjects' physiological and perceptual responses were recorded. Twenty-four hours later, subjects began a cold acclimation protocol, which consisted of seven, 90-minute immersions in cold water (10°C, water level to chest). Each immersion was also separated by 24 hours. Subjects then repeated a subsequent trial of exercise in the heat 24 hours after the final immersion of the cold acclimation protocol. RESULTS: Results from cold acclimation revealed no change in core temperature, a decrease in skin temperature, and attenuated shivering and lactate responses, which supports a successful insulative-hypothermic cold acclimation response. This type of cold acclimation response primarily results in heat retention with associated energy conservation. Findings for heat trials (pre-cold acclimation and post-cold acclimation) revealed no differences between trials for all measurements, suggesting that cold acclimation did not influence physiological or perceptual responses during exercise in the heat. CONCLUSION: Our findings indicate that military divers or trainees that are frequently exposed to cold water, and hence have the ability to experience cold acclimatization, will likely not be at greater risk of increased thermal strain when subsequently exposed to physical activity in hot environments. In this study, no physiological or perceptual differences were observed between trials before and after cold acclimation, suggesting that cold acclimation does not present a greater hyperthermia risk during subsequent exercise in the heat.
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Aclimatação/fisiologia , Temperatura Baixa , Percepção/fisiologia , Esforço Físico/fisiologia , Sensação/fisiologia , Adulto , Análise de Variância , Feminino , Temperatura Alta , Humanos , MasculinoRESUMO
The time necessary for the initial appearance of ingested water as sweat during exercise in the heat remains unknown. Based on the current literature, we estimated fluid transition through the body, from ingestion to appearance as sweat, to have a minimum time duration of approximately three minutes. The purpose of this study was to test this prediction and identify the time necessary for the initial enrichment of deuterium oxide (D2O) in sweat following ingestion during exercise in the heat. Eight participants performed moderate intensity (40% of maximal oxygen uptake) treadmill exercise in an environmental chamber (40°C, 40% rH) to induce active sweating. After fifteen minutes, while continuing to walk, participants consumed D2O (0.15mlkg-1) in a final volume of 50ml water. Scapular sweat samples were collected one minute prior to and ten minutes post-ingestion. Samples were analyzed for sweat D2O concentration using isotope ratio mass spectrometry and compared to baseline. Mean±SD ∆ sweat D2O concentration at minutes one and two post-ingestion were not significantly higher than baseline (0min). Minutes three (9±3ppm) through ten (23±11ppm) post-ingestion had ∆ sweat D2O concentrations significantly (P<0.05) higher than baseline. Such results suggest that ingested water rapidly transports across the mucosal membrane of the alimentary canal into the vasculature space, enters the extravascular fluid, and is actively secreted by the eccrine sweat glands onto the surface of the skin for potential evaporation in as little as three minutes during exercise in the heat.
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Óxido de Deutério/farmacocinética , Glândulas Écrinas/metabolismo , Exercício Físico , Temperatura Alta , Sudorese , Administração Oral , Adolescente , Adulto , Óxido de Deutério/administração & dosagem , Feminino , Humanos , Masculino , Consumo de Oxigênio , Suor/metabolismoRESUMO
BACKGROUND: The purpose of this study was to determine if K+ is leached from the stratum corneum when sweat is present on the skin's surface. The results will help address whether sweat [K+] previously reported in the literature are artifactually elevated as a result of K+ leaching. METHODS: Twelve (six female, six male) healthy volunteers participated in this study. After thorough skin cleansing and preparation with isopropyl alcohol and high-performance liquid chromatography-grade distilled water, three sites were chosen and a 50 µL drop of artificial sweat was pipetted directly onto the skin. The artificial sweat had a [K+] of 4 mEq·L-1, an osmolality of 120 mosm·L-1, and a pH of 6.0. Immediately following, a clear plastic cover slip (~6 cm2) with a shallow 0.8 cm2 convex impression in the center was applied over each drop, preventing evaporation. Each sample was allowed to sit on the forearm, under the plastic cover slip, for 10 min. RESULTS: The mean (±SD) [K+] in 'artificial' sweat not exposed to the skin was measured to be 4.2±0.4 mEq·L-1. After 10 min of exposure to the stratum corneum of the forearm, the artificial sweat had a mean (±SD) [K+] of 3.9±0.3 mEq·L-1. There was no significant difference (p>0.05) in the [K+] between the control artificial sweat and the samples collected after 10 min of exposure to forearm skin. CONCLUSIONS: These results do not support the hypothesis that significant K+ leaching from the stratum corneum into standing sweat is the cause for the previously reported elevated sweat [K+].
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Potássio/metabolismo , Pele/metabolismo , Suor/metabolismo , Adulto , Feminino , Humanos , Masculino , Absorção Cutânea/fisiologiaRESUMO
NEW FINDINGS: What is the central question of this study? The purpose of the present study was to determine the effects of exercise-induced haemoconcentration and hyperthermia on blood viscosity. What is the main finding and its importance? Exercise-induced haemoconcentration, increased plasma viscosity and increased blood aggregation, all of which increased blood viscosity, were counterbalanced by increased red blood cell (RBC) deformability (e.g. RBC membrane shear elastic modulus and elongation index) caused by the hyperthermia. Thus, blood viscosity remained unchanged following prolonged moderate-intensity exercise in the heat. Previous studies have reported that blood viscosity is significantly increased following exercise. However, these studies measured both pre- and postexercise blood viscosity at 37 °C even though core and blood temperatures would be expected to have increased during the exercise. Consequently, the effect of exercise-induced hyperthermia on mitigating change in blood viscosity may have been missed. The purpose of this study was to isolate the effects of exercise-induced haemoconcentration and hyperthermia and to determine their combined effects on blood viscosity. Nine subjects performed 2 h of moderate-intensity exercise in the heat (37 °C, 40% relative humidity), which resulted in significant increases from pre-exercise values for rectal temperature (from 37.11 ± 0.35 to 38.76 ± 0.13 °C), haemoconcentration (haematocrit increased from 43.6 ± 3.6 to 45.6 ± 3.5%) and dehydration (change in body weight = -3.6 ± 0.7%). Exercise-induced haemoconcentration significantly (P < 0.05) increased blood viscosity by 9% (from 3.97 to 4.33 cP at 300 s(-1)), whereas exercise-induced hyperthermia significantly decreased blood viscosity by 7% (from 3.97 to 3.69 cP at 300 s(-1)). When both factors were considered together, there was no overall change in blood viscosity (from 3.97 to 4.03 cP at 300 s(-1)). The effects of exercise-induced haemoconcentration, increased plasma viscosity and increased red blood cell aggregation, all of which increased blood viscosity, were counterbalanced by increased red blood cell deformability (e.g. red blood cell membrane shear elastic modulus and elongation index) caused by the hyperthermia. Thus, blood viscosity remained unchanged following prolonged moderate-intensity exercise in the heat.
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Viscosidade Sanguínea/fisiologia , Temperatura Corporal/fisiologia , Exercício Físico/fisiologia , Adulto , Agregação Celular/fisiologia , Desidratação/fisiopatologia , Membrana Eritrocítica/fisiologia , Eritrócitos/fisiologia , Feminino , Febre/fisiopatologia , Hematócrito/métodos , Temperatura Alta , Humanos , MasculinoRESUMO
Anapyrexia is the regulated decrease in body temperature during acute exposure to hypoxia. This study examined resting rectal temperature (Trec) in adult humans during acute normobaric hypoxia (NH). Ten subjects breathed air consisting of 21% (NN), 14% (NH14), and 12% oxygen (NH12) for 30 min each in thermoneutral conditions while Trec and blood oxygen saturation (Spo2) were measured. Linear regression indicated that Spo2 was progressively lower in NH14 (p=0.0001) and NH12 (p=0.0001) compared to NN, and that Spo2 in NH14 was different than NH12 (p=0.00001). Trec was progressively lower during NH14 (p=0.014) and in NH12 (p=0.0001) compared to NN. The difference in Trec between NH14 and NH12 was also significant (p=0.0287). Spo2 was a significant predictor of Trec such that for every 1% decrease in Spo2, Trec decreased by 0.15°C (p=0.0001). The present study confirmed that, similar to many other species, human adults respond to acute hypoxia exposure by lowering rectal temperature.
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Regulação da Temperatura Corporal/fisiologia , Hipóxia/fisiopatologia , Aclimatação/fisiologia , Adulto , Feminino , Humanos , Hipóxia/sangue , Masculino , Oximetria , Oxigênio/sangue , Consumo de Oxigênio/fisiologia , Reto/fisiologia , Termometria/métodosRESUMO
The purpose of this study was to determine the effect of alterations in rate of limb movement on Phase III ventilation during exercise, independent of metabolic rate, gait style, and treadmill incline. Subjects completed five submaximal exercise bouts on a lower body positive pressure treadmill (AlterG P 200). The percent body weight for the five exercise bouts was 100, 87, 75, 63, and 50% and each was matched for carbon dioxide production (V CO2 ). Naturally, to match the V CO2 while reducing the body weight up to 50% of normal required a significant increase in the treadmill speed from 3.0 ± 0.1 to 4.1 ± 0.2 mph, which resulted in a significant (P < 0.05) increase in the mean step frequency (steps per minute) from 118 ± 10 at 3 mph (i.e., 100% of body weight) to 133 ± 6 at 4.1 mph (i.e., 50% of body weight). The most important finding was that significant increases in step frequency did not significantly alter minute ventilation or respiratory rate. Such results do not support an important role for the rate of limb movement in Phase III ventilation during submaximal exercise, when metabolic rate, gait style, and treadmill incline are controlled.
Assuntos
Teste de Esforço/métodos , Perna (Membro)/fisiologia , Movimento/fisiologia , Respiração , Taxa Respiratória/fisiologia , Adulto , Peso Corporal , Exercício Físico/fisiologia , Feminino , Humanos , MasculinoRESUMO
CONTEXT: Bikram yoga has gained a large following, possibly because of widespread claims boasting energy expenditure of up to 1000 calories per session. However, these claims are unfounded because no scientific study has investigated the metabolic response to a complete, standardized Bikram yoga class. OBJECTIVES: This study intends to determine energy expenditure, heart rate, and sweat rate in novice and experienced practitioners from a standardized Bikram yoga class. SETTING: Data were collected in the environmental chamber of the Exercise Physiology Laboratory at San Diego State University in California, USA. PARTICIPANTS: Male (n = 5) and female (n = 19) participants between the ages of 18 and 57 y were recruited through flyers in yoga studios throughout San Diego. Participants were classified as experienced or novice practitioners, having completed ≥20 or <20 sessions, respectively. INTERVENTIONS: Participants were guided through a standardized 90-min yoga class performed in a hot environment using Bikram's Standard Beginning Dialogue, while expired gas was collected and heart rate was recorded. OUTCOME MEASURES: Energy expenditure, calculated via oxygen uptake, and heart rate were determined for each posture and transition period. In addition, sweat rate and core temperature were recorded for each participant. RESULTS: Mean (±SD) relative VO2 for the entire 90-min session was 9.5 ± 1.9 mL × kg-1 × min-1, ranging from 6.0 to 12.9 mL × kg-1 × min-1. Mean absolute energy expenditure was 286 ± 72 kcals, ranging from 179 to 478 kcals. Independent sample t tests revealed significant differences (P < .05) in relative energy expenditure, heart rate, ending core temperature, and sweat rate between experience levels. Mean relative energy expenditure was 3.7 ± 0.5 kcal/kg in novice practitioners and 4.7 ± 0.8 kcal/kg in experienced practitioners. Percentage of predicted maximum heart rate and sweat rate were 72.3% ± 10.6% and 0.6 ± 0.2 kg/h in novice practitioners and 86.4% ± 5.2% and 1.1 ± 0.5 kg/h in experienced participants. All postures were classified as light-to-moderate intensity according to the American College of Sports Medicine (ACSM) standards. CONCLUSIONS: Bikram yoga meets requirements for exercise of light-to-moderate intensity and, theoretically, could be used for weight maintenance or weight loss if practiced several times per week.