1.
Vopr Virusol
; 43(4): 187-90, 1998.
Artigo
em Russo
| MEDLINE
| ID: mdl-9791886
RESUMO
Sensitivity and specificity of the Russian PCR test system (experimental lot) were assessed with materials from 107 Russian patients with HIV infection and 100 donors without specific antibodies to HIV-1. The electrophoretic and hybridization-enzymatic methods for amplicon detection were compared. The sensitivity of electrophoresis was 89.9%, specificity 91%; for hybridization-enzymatic detection these values were 96.1 and 100%, respectively. Samples which yielded false-negative results were repeatedly tested by two-staged amplification (nested PCR) with external and internal primers. The main cause of false-negative results was a low concentration of proviral HIV-1 DNA in some blood samples.