RESUMO
The aim of this work was the establishment and characterization of porcine aortic endothelial cell cultures with prolonged lifespan. Endothelial cells where isolated from porcine thoracic aorta and established in primary culture; after sub cultivation, the cells showed typical morphology of endothelial phenotype with cobblestone appearance and growth in monolayer; they were positive against anti-CD31 and anti-CD54 immunostaining and Ac-LDL-Dil uptake. The cells were able to migrate in culture and showed a normal growth curve. The phenotype of our in vitro model of endothelial cells was stable through subcultivation; so, it should be a valuable tool for diverse studies of the endothelial response against physiological and pathological stimulus, particularly it could be useful to study interactions of endothelium with pathogenic bacteria causing diseases in pigs, as they are difficult to study in vivo.
Assuntos
Aorta/citologia , Técnicas de Cultura de Células/métodos , Células Endoteliais/citologia , Suínos , Animais , Proliferação de Células , Células CultivadasRESUMO
It has been hypothesized that the hormonal status is involved in the distinct susceptibility between men and women towards the development of hepatobiliary disease because the liver is target organ for steroid hormones. In the development of the fibrogenic phenomenon present in the hepatic cirrhosis of diverse etiology, Ito cells, myofibroblasts as well as some cytokines like transforming growth factor-beta, interleukin-6 and tumor necrosis factor-alpha are involved. It is known that in the organism exist regulatory loops among cytokines, glucocorticoids and sex steroids; in the liver this interaction could affect the fibrogenic phenomenon through Ito cells differentiation. The knowledge of the precise role of glucocorticoids and sex steroids on the fibrogenic mechanisms should allow to support rationally the viability of hormone manipulation for the treatment of hepatic diseases of fibrotic nature.