Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 34
Filtrar
1.
Br J Cancer ; 86(5): 702-4, 2002 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-11875729

RESUMO

Epstein-Barr virus has been associated with a proportion of typical gastric adenocarcinomas. Here we report that the prevalence of Epstein-Barr virus in gastric adenocarcinomas from the United Kingdom is one of the lowest in the World. Gastric adenocarcinoma is another tumour whose association with Epstein-Barr virus varies with the population studied.


Assuntos
Adenocarcinoma/etiologia , Adenocarcinoma/virologia , Infecções por Vírus Epstein-Barr/complicações , Neoplasias Gástricas/etiologia , Neoplasias Gástricas/virologia , Adenocarcinoma/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos Epidemiológicos , Infecções por Vírus Epstein-Barr/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Neoplasias Gástricas/epidemiologia , Reino Unido/epidemiologia
2.
Clin Diagn Lab Immunol ; 8(6): 1120-5, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11687450

RESUMO

The antibody response patterns of cattle after subcutaneous and intranasal immunizations with adhesin Tf190 of Tritrichomonas foetus were investigated. Reactions of antibody from cattle parenterally immunized with Tf190 revealed antigen specificity and Tf190 sensitization in the majority of the animals, as determined by Western blotting. The results also demonstrated strong preimmune immunoglobulin G2 (IgG2) binding to T. foetus antigens not seen in IgG1 profiles. Subcutaneous injections of Tf190 resulted in significant (P < 0.05) increases in serum IgG1 and IgG2 titers over time, as determined by parasite specific enzyme-linked immunosorbent assay. Immune sera also significantly inhibited parasite adhesion to mammalian cell lines compared to the level of inhibition obtained with preimmune sera (P < 0.05). Intranasal immunization with Tf190 failed to produce measurable parasite-specific antibody in serum; however, this immunization route did result in significant (P < 0.05) increases in parasite-specific IgA titers in cervical mucus secretions from immunized animals that were more resistant to intravaginal challenge with T. foetus than controls. These results suggest that systemic immunization with Tf190 results in serum antibody production and antiparasitic adhesin antibodies. Additionally, the results of challenge experiments with intranasally immunized animals suggests that Tf190 primes protective immune responses that lead to lower rates of infection among these animals.


Assuntos
Adesinas Bacterianas/imunologia , Doenças dos Bovinos/prevenção & controle , Infecções por Protozoários/prevenção & controle , Proteínas de Protozoários/imunologia , Tritrichomonas foetus/imunologia , Animais , Anticorpos Antiprotozoários/sangue , Bovinos , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/parasitologia , Colo do Útero/imunologia , Colo do Útero/parasitologia , Ensaio de Imunoadsorção Enzimática , Feminino , Imunização , Imunoglobulina A/sangue , Infecções por Protozoários/imunologia
3.
J Parasitol ; 87(5): 1040-8, 2001 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11695362

RESUMO

The cellular immune responses of cattle immunized with antigens of Tritrichomonas foetus were investigated. Subcutaneous injections of antigen preparations primed bovine peripheral blood mononuclear cells (PBMC) by 30 days of immunization as demonstrated by antigen-specific proliferation and by cytokine production upon antigen challenge of PBMC. Antigen-specific T-cells derived from PBMC responded by production of interferon (IFN)-gamma message detected by reverse transcriptase polymerase chain reaction, secreted IFN-gamma detected by enzyme-linked immunosorbent assay, and intracellular IFN-gamma detected by flow cytometry. Phenotypic analysis of PBMC responding in vitro to parasite antigen demonstrated a shift from a mixed CD4+, CD8+, gammadelta+, to predominantly CD4+, CD8-, gammadelta- phenotype in the Tf190-primed PBMC. In conclusion, systemic immunization of cattle with parasite antigen results in priming of bovine T-cells that are antigen specific and can produce an anamnestic IFN-gamma response to subsequent stimulation with antigens of T. foetus.


Assuntos
Antígenos de Protozoários/imunologia , Doenças dos Bovinos/imunologia , Infecções Protozoárias em Animais , Linfócitos T/imunologia , Tritrichomonas foetus/imunologia , Animais , Antígenos de Protozoários/administração & dosagem , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/parasitologia , Adesão Celular , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Citometria de Fluxo/veterinária , Imunização/veterinária , Interferon gama/biossíntese , Interferon gama/genética , Interferon gama/farmacologia , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/imunologia , Ativação Linfocitária/imunologia , Subpopulações de Linfócitos/imunologia , Masculino , Monócitos , Gravidez , Infecções por Protozoários/imunologia , Infecções por Protozoários/parasitologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Linfócitos T/química , Tritrichomonas foetus/crescimento & desenvolvimento , Regulação para Cima
5.
J Anim Sci ; 78(10): 2731-6, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11048940

RESUMO

Fifty-two Targhee twin-bearing ewes were used in a factorial arrangement of treatments to investigate the role of supplemental vitamin E (vit E); 0 (NE) vs 400 IU of vit E x ewe x (-1)d(-1) (E) and parainfluenza type 3 (PI3) vaccination; none (NP) vs PI3 vaccination (P) in immune function. Parainfluenza type 3 vaccination was used to evoke an immune response. Ewes receiving PI3 were vaccinated at 49 and 21 d before the expected lambing date. Ewes receiving vit E were orally dosed daily, 32 to 0 d before lambing. Blood was collected from ewes at the time of the initial PI3 vaccination and 4 h postpartum. Blood was collected from lambs (n = 104) at 3 d postpartum. Ewe and lamb sera were analyzed for anti-PI3 antibody titers, immunoglobulin G (IgG) titers, and vit E concentrations. Colostrum was collected 4 h postpartum and analyzed for IgG. The model for ewe and lamb analysis included the main effects of vit E and PI3, sex (lambs model only), and their interactions. No interactions were detected (P > 0.20) for any ewe or lamb variables. Serum anti-PI3 titers were greater (P < 0.01) in P ewes and their lambs than NP ewes and their lambs. Serum vit E concentrations were greater (P < 0.01) in E ewes and their lambs than NE ewes and their lambs. Colostral IgG titers and serum anti-PI3 titers did not differ (P > 0.20) between E and NE ewes. Serum IgG titers in E ewes and their lambs did not differ (P > 0.15) from IgG titers in NE ewes and their lambs. Lamb anti-PI3 titers did not differ (P = 0.76) between lambs reared by E and NE ewes. These results indicate that, although supplemental vit E to the ewe increased lamb serum vit E concentration, it had no effect on measures used in this study to assess humoral immunity in the ewe or passive immunity to the lamb.


Assuntos
Ovinos/imunologia , Vitamina E/farmacologia , Animais , Anticorpos Antivirais/biossíntese , Fibras na Dieta/metabolismo , Suplementos Nutricionais , Grão Comestível/metabolismo , Feminino , Imunização Passiva/veterinária , Tamanho da Ninhada de Vivíparos , Respirovirus/imunologia , Vacinação/veterinária
6.
Am J Physiol Heart Circ Physiol ; 279(3): H1299-306, 2000 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10993797

RESUMO

Blood pressure contains a distinct low-frequency oscillation often termed the Mayer wave. This oscillation is caused by the action of the sympathetic nervous system on the vasculature and results from time delays in the baroreflex feedback loop for the control of sympathetic nerve activity (SNA) in response to changes in blood pressure. In this study, we used bilateral renal denervation to test the hypothesis that it is SNA to the kidney that contributes a large portion of the vascular resistance associated with changes in the strength of the slow oscillation in blood pressure. In conscious rabbits, SNA and blood pressure were measured during hemorrhage (blood withdrawal at 1.35 ml. min(-1). kg(-1) for 20 min). Spectral analysis identified a strong increase in power at 0.3 Hz in SNA and blood pressure in the initial compensatory phase of hemorrhage before blood pressure started to fall. However, in a separate group of renal denervated rabbits, although the power of the 0.3-Hz oscillation under control conditions in blood pressure was similar, it was not altered during hemorrhage. Wavelet analysis revealed the development of low-frequency oscillations at 0.1 Hz in both intact and denervated animals. In conclusion, we propose that changes in the strength of the oscillation at 0.3 Hz in arterial pressure during hemorrhage are primarily mediated by sympathetic activity directed to the kidney.


Assuntos
Pressão Sanguínea/fisiologia , Hemorragia/fisiopatologia , Rim/inervação , Periodicidade , Sistema Nervoso Simpático/fisiologia , Animais , Barorreflexo/fisiologia , Rim/fisiologia , Rim/cirurgia , Oscilometria , Coelhos , Processamento de Sinais Assistido por Computador , Simpatectomia , Vigília/fisiologia
7.
Vet Pathol ; 36(5): 406-11, 1999 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10490208

RESUMO

Portions of penis and prepuce were collected from 24 bulls with current or recent Tritrichomonas foetus infection. Epididymides were collected from seven of the bulls, and seminal vesicles and prostate were collected from four. Following immunohistochemical staining with two monoclonal antibodies (34.7C4.4 and TF1.15) prepared against T. foetus surface antigens, trichomonads were identified in sections from 15 of the bulls. Organisms were most often located in penile crypts in the midshaft and caudal regions and less often in preputial crypts. Trichomonads were not observed in sections from other genitalia or in subepithelial tissue. T. foetus antigen, however, was present in the cytoplasm of some epithelial cells and the cytoplasm of some mononuclear cells in subepithelial lymphoid aggregates and follicles. Preputial smegma was collected from 16 T. foetus-infected bulls and from 16 control bulls with negative T. foetus cultures. Preputial antibody levels to TF1.17, a surface antigen of T. foetus, were determined by an enzyme-linked immunosorbent assay. Preputial secretions from infected bulls contained specific antibody of each isotype and subisotype tested. IgG1 responses were the greatest, IgM and IgA responses were approximately equal, and IgG2 responses were low. Each isotype and subisotype response in infected bulls was significantly greater than that in the controls. These results confirm previous speculation concerning anatomical sites of infection and suggest that parasite antigen can be taken up and processed locally, resulting in deposition of specific IgG1, IgG2, IgA, and IgM antibodies in the preputial cavity.


Assuntos
Anticorpos Antiprotozoários/análise , Doenças dos Bovinos/parasitologia , Pênis/parasitologia , Infecções Protozoárias em Animais , Tritrichomonas foetus/isolamento & purificação , Animais , Anticorpos Monoclonais , California , Bovinos , Ensaio de Imunoadsorção Enzimática/veterinária , Epididimite/parasitologia , Imunoglobulinas/análise , Imuno-Histoquímica , Masculino , New Mexico , Próstata/parasitologia , Infecções por Protozoários/imunologia , Infecções por Protozoários/parasitologia , Saskatchewan , Glândulas Seminais/parasitologia , Esmegma/imunologia , Esmegma/parasitologia , Tritrichomonas foetus/imunologia
8.
Am J Physiol ; 277(3): R894-903, 1999 09.
Artigo em Inglês | MEDLINE | ID: mdl-10484509

RESUMO

The object of this study is to quantify the very low frequency (i.e., <0.1 Hz) interactions between renal sympathetic nerve activity (SNA) and arterial blood pressure (ABP). Six rats were instrumented for chronic recordings of SNA and ABP. Data were collected 24 h after surgery at 10 kHz for 2-5 h and subsequently compressed to a 1-kHz signal. The power spectra and ordinary coherence were calculated from data epochs up to 1 h in length. The very low frequency spectra for both variables were fitted to a constant times f (-beta). The peak magnitude squared of the coherence near 0.4 Hz was 0.82 +/- 0.08, but the apparent linear coherence fell off quickly at lower frequencies so that it was close to zero for frequencies <0.1 Hz. Moreover, at these low frequencies beta, as computed by a coarse grain spectral analysis, was significantly (P < 0.01) different for SNA (0.66 +/- 0.12) and ABP (1.12 +/- 0.14). Assuming that SNA and ABP are stationary time series, the results of our classical spectral analysis would indicate that SNA and ABP are not linearly correlated at frequencies with a period more than approximately 10 s. Accordingly, we tested for stationarity by computing the spectral coherence and found that SNA and ABP are not stationary "1/f noise" within the frequency range from 0.02 to 2.0 Hz. Rather the SNA exerts control over the cardiovascular system through intermittent bursts of activity. Such intermittent behavior can be modeled by nonlinear dynamics.


Assuntos
Barorreflexo/fisiologia , Pressão Sanguínea/fisiologia , Rim/fisiologia , Sistema Nervoso Simpático/fisiologia , Animais , Rim/inervação , Ratos , Ratos Sprague-Dawley
9.
Infect Immun ; 66(3): 1100-5, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9488401

RESUMO

Bovine trichomoniasis is a sexually transmitted disease caused by Tritrichomonas foetus and characterized by early embryo loss. The mechanism of this loss is not known, although the parasite is known to cause inflammation and to have the ability to kill host cells by a contact-dependent cytotoxic mechanism. Antibody specific for a 190,000-Da surface complex (Tf190) was previously shown to inhibit this adhesion. In this study we used immunoaffinity chromatography to purify Tf190 from T. foetus in order to analyze its composition and examine its expression. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified Tf190 followed by silver staining revealed three components of Tf190. Western blotting and antibody-binding experiments showed that the 140- and 60-kDa bands were immunogenic. By using a battery of monoclonal antibodies (MAbs) periodate-sensitive epitopes were identified on Tf190, suggesting that these epitopes contained carbohydrate structures. Analyses of affinity-purified Tf190 by high-performance liquid chromatography and gas-liquid chromatography demonstrated the presence of the monosaccharides and lipids known to be prominent constituents of the lipophosphoglycan (LPG) of T. foetus. Flow cytometry experiments on several isolates of T. foetus with Tf190-specific antibodies revealed that Tf190 was present on subpopulations of all isolates but that not all epitopes were present on every isolate. This pattern of reactivities on the different parasite isolates was confirmed by Western blots of whole-parasite extracts probed with MAbs and antiserum. These results suggest that although variation in the expression of epitopes of Tf190 occurs in different strains of T. foetus, the Tf190 adhesion complex is widespread in different populations of the parasite. The data further suggest that immunogenic structures, important in the adhesion of T. foetus to mammalian cells, are located in the LPG-like component of Tf190.


Assuntos
Proteínas de Protozoários/isolamento & purificação , Tritrichomonas foetus/química , Animais , Anticorpos Monoclonais/imunologia , Bovinos , Adesão Celular , Cromatografia de Afinidade , Epitopos , Citometria de Fluxo , Glicoesfingolipídeos/fisiologia , Proteínas de Protozoários/análise , Proteínas de Protozoários/imunologia , Coelhos
10.
Am J Physiol ; 273(3 Pt 2): R851-7, 1997 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-9321859

RESUMO

The goal of this analysis was to quantify the relationship between renal sympathetic nerve activity (SNA) and mean arterial blood pressure (MAP). We previously recorded renal SNA and MAP in conscious rats during a stressful behavioral stimulus and during a nonstressful stimulus. We then formulated a set of two linear, first-order differential equations that uses our SNA recordings after a time delay (the input) to predict fluctuations in MAP (the output). Our model has four parameters: 1) the cardiovascular time constant T that characterizes the frequency response function between the effector elements controlled by the sympathetic nerves and the cardiovascular system (1-5 s); 2) the effector time constant Te determined by the coupling between the sympathetic nervous system and the effectors (0.0-0.6 s); 3) the efferent time delay tau e between a change in SNA and a change in MAP (0.4-0.6 s); and 4) a proportionality constant C between fluctuations in SNA and fluctuations in MAP (0.3-3.4 mmHg/nV). The parameters of the model were determined that minimize the residual error between the simulated time series and the actual data time series for a stressful stimulus. Then we tested the ability of the transfer function to predict the MAP response to a nonstressful stimulus. In five of seven rats tested, the model's predictions were good, with mean cross-correlation coefficients for the predicted trials between 0.62 and 0.83. We show that multifiber renal SNA recordings can reliably predict changes in MAP in the unanesthetized rat. Thus the overall sympathetic drive to the cardiovascular system is indexed by renal SNA, although the vasomotor effectors driven by renal SNA control only approximately 20% of the blood cow.


Assuntos
Pressão Sanguínea , Rim/inervação , Estresse Psicológico/fisiopatologia , Sistema Nervoso Simpático/fisiologia , Animais , Condicionamento Psicológico , Eletrochoque , Homeostase , Modelos Biológicos , Ratos , Ratos Sprague-Dawley , Restrição Física , Fatores de Tempo
11.
Am J Physiol ; 273(6): R1878-84, 1997 12.
Artigo em Inglês | MEDLINE | ID: mdl-9435640

RESUMO

We have described a 0.4-Hz rhythm in renal sympathetic nerve activity (SNA) that is tightly coupled to 0.4-Hz oscillations in blood pressure in the unanesthetized rat. In previous work, the relationship between SNA and fluctuations in mean arterial blood pressure (MAP) was described by a set of two first-order differential equations. We have now modified our earlier model to test the feasibility that the 0.4-Hz rhythm can be explained by the baroreflex without requiring a neural oscillator. In this baroreflex model, a linear feedback term replaces the sympathetic drive to the cardiovascular system. The time delay in the feedback loop is set equal to the time delay on the efferent side, approximately 0.5 s (as determined in the initial model), plus a time delay of 0.2 s on the afferent side for a total time delay of approximately 0.7 s. A stability analysis of this new model yields feedback resonant frequencies close to 0.4 Hz. Because of the time delay in the feedback loop, the proportional gain may not exceed a value on the order of 10 to maintain stability. The addition of a derivative feedback term increases the system's stability for a positive range of derivative gains. We conclude that the known physiological time delay for the sympathetic portion of the baroreflex can account for the observed 0.4-Hz rhythm in rat MAP and that the sensitivity of the baroreceptors to the rate of change in blood pressure, as well as average blood pressure, would enhance the natural stability of the baroreflex.


Assuntos
Ciclos de Atividade/fisiologia , Barorreflexo/fisiologia , Pressão Sanguínea/fisiologia , Rim/inervação , Animais , Estudos de Viabilidade , Retroalimentação , Modelos Biológicos , Modelos Estatísticos , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Sistema Nervoso Simpático/fisiologia
12.
J Vet Diagn Invest ; 7(1): 98-101, 1995 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-7779973

RESUMO

An immunohistochemical technique using a monoclonal antibody was evaluated as a diagnostic tool to specifically label Tritrichomonas foetus in formalin-fixed, paraffin-embedded sections of placenta and fetal lung from bovine abortions. Trichomonads were demonstrated in tissues from each of 12 abortions due to T. foetus and none of 15 abortions due to other or unidentified causes. Moderate to marked background staining occurred only in severely autolyzed tissues from T. foetus-infected fetuses. The antibody faintly labeled 1 of 3 other species of trichomonads (Trichomonas gallinae) but did not label other protozoa, bacteria, or fungi tested.


Assuntos
Aborto Animal/microbiologia , Doenças dos Bovinos , Pulmão/microbiologia , Placenta/microbiologia , Infecções Protozoárias em Animais , Tritrichomonas foetus/isolamento & purificação , Aborto Animal/patologia , Animais , Bovinos , Vilosidades Coriônicas/microbiologia , Vilosidades Coriônicas/patologia , Feminino , Feto , Imuno-Histoquímica/métodos , Pulmão/embriologia , Pulmão/patologia , Placenta/patologia , Gravidez , Infecções por Protozoários/patologia
13.
Hosp J ; 9(1): 1-12, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-8406398

RESUMO

Genograms are a valuable and non-threatening evaluation tool for hospice patients and families. The genogram provides basic information about the family including the role of each member and the family dynamics. As the diagram is drawn, family life cycle issues and relationships between family members become evident. The genogram may go beyond the household to include supportive neighbors, friends, and community resources. Religious and spiritual support is also noted. The information is used to assess family needs and to provide interventions both before the death and during bereavement.


Assuntos
Família/psicologia , Cuidados Paliativos na Terminalidade da Vida/psicologia , Avaliação em Enfermagem , Linhagem , Cuidadores , Humanos , Relação entre Gerações , Apoio Social , Serviço Social
14.
Infect Immun ; 60(10): 4253-9, 1992 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-1398936

RESUMO

The relationship of Tritrichomonas foetus adhesion to mammalian cells and cytotoxicity to these targets was investigated. High-adherence and low-adherence lines of T. foetus, derived by repeated adhesion to HeLa cells, showed high and low cytotoxicity, respectively, to HeLa cells. When parasites were separated from targets by membranes (0.4-microns pore size), no cytotoxicity was detectable. Monoclonal antibodies elicited against T. foetus that lowered adhesion also lowered parasite-mediated cytotoxicity. Flow cytometry experiments revealed that the levels of an adhesion- and cytotoxicity-blocking antibody bound to the surface of high-adherence clones of T. foetus were higher than those in low-adherence clones. Western blots of parasite extracts separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were probed with an anti-T. foetus antibody. A molecule with a molecular weight of approximately 190,000 composed of subunits with molecular weights of approximately 140,000 to 150,000 and approximately 65,000 was identified. Immunoprecipitation experiments with metabolically labeled T. foetus and the same antibody confirmed that similar subunits were synthesized by the parasite. These results indicate that adhesion of T. foetus to mammalian cells is an important step in cytotoxic damage of these targets and that a surface adhesin on the parasite is involved in the adhesion mechanism.


Assuntos
Anticorpos Monoclonais/imunologia , Tritrichomonas foetus/patogenicidade , Adesividade , Animais , Feminino , Células HeLa , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Peso Molecular , Proteínas de Protozoários/análise , Ratos
15.
Infect Immun ; 58(11): 3627-32, 1990 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-2228233

RESUMO

Geographically distinct lines of Tritrichomonas foetus were assayed for their ability to cause cytotoxicity in nucleated mammalian cells and lysis of bovine erythrocytes. T. foetus was highly cytotoxic toward a human cervical cell line (HeLa) and early bovine lymphosarcoma (BL-3) but displayed low levels of cytotoxicity against African green monkey kidney (Vero) cells. In addition to variation in the extent of cytotoxicity toward different targets, differences in the levels of cytotoxicity in the same nucleated target occurred with different parasite lines. Whole T. foetus, unfractionated whole-cell extracts, and parasite-conditioned medium (RPMI 1640 without serum) all caused lysis of bovine erythrocytes. Lytic activity in the conditioned medium was substantially reduced by repeated freezing and thawing or heating to 90 degrees C for 30 min. Damage of mammalian target cells by live T. foetus could be reduced by the presence of protease inhibitors; however, such inhibitors did not diminish the lytic effects of conditioned medium. These results suggested that proteolytic enzymes were necessary for the lytic mechanism of the live parasites but were not required once lytic factors were released into the parasite-conditioned medium. They further suggested that the lytic molecules were either proteins or had proteinaceous components.


Assuntos
Células Cultivadas/parasitologia , Hemólise , Infecções por Protozoários/patologia , Tritrichomonas/fisiologia , Animais , Endopeptidases/farmacologia , Congelamento , Temperatura Alta , Humanos
17.
J Parasitol ; 75(6): 977-80, 1989 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-2693679

RESUMO

Sections of bovine placenta from cases of bovine trichomoniasis were examined for the presence of Tritrichomonas foetus by standard histological methods using phase-contrast microscopy and by indirect immunofluorescence assay (IFA) employing monoclonal antibodies (mAbs) specific for T. foetus. Parasites were identified readily in deparaffinized tissue up to 4 yr old by IFA with 2 mAbs previously shown to bind to the surface of living T. foetus. These results indicated that the IFA provided a rapid and specific method of identifying T. foetus in tissue sections as compared to standard histological methods.


Assuntos
Aborto Animal/parasitologia , Doenças dos Bovinos/parasitologia , Placenta/parasitologia , Infecções Protozoárias em Animais , Tritrichomonas/isolamento & purificação , Animais , Anticorpos Monoclonais/imunologia , Bovinos , Reações Cruzadas , Feminino , Imunofluorescência , Microscopia de Contraste de Fase , Gravidez , Infecções por Protozoários/parasitologia , Tritrichomonas/imunologia
18.
J Parasitol ; 74(5): 828-32, 1988 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-2458442

RESUMO

Sporozoites and culture-derived merozoites of Sarcocystis cruzi were used to elicit monoclonal antibodies (MAb's) in mice. Some of these antibodies reacted with the surface of live sporozoites and merozoites as determined by immunofluorescence. An array of similar antigens was identified in Western blots of sporozoites by both anti-merozoite MAb's and an anti-sporozoite MAb. At least 1 antigen in blots of bradyzoites was identified by anti-merozoite MAb's and a cluster of antigens was identified by an anti-sporozoite antibody. These results indicate that several surface epitopes of sporozoites and merozoites are shared with molecules of bradyzoites and that antigen patterns of molecules bearing these epitopes in 3 stages of Sarcocystis may be either distinct or similar.


Assuntos
Antígenos de Protozoários/análise , Sarcocystis/imunologia , Animais , Anticorpos Monoclonais , Antígenos de Superfície/análise , Eletroforese em Gel de Poliacrilamida , Epitopos , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Sarcocystis/crescimento & desenvolvimento
19.
Infect Immun ; 56(10): 2538-43, 1988 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-3047060

RESUMO

Five monoclonal antibodies (MAbs) were partially characterized and tested for their ability to inhibit penetration of Madin-Darby bovine kidney (MDBK) cells by sporozoites of Eimeria bovis. By indirect fluorescent-antibody assays, all MAbs reacted with acetone-fixed sporozoites, but only two MAbs, EbS9 (immunoglobulin G1) and EbS11 (immunoglobulin G2a), localized specifically on the plasmalemma of live sporozoites. Two of the five MAbs also reacted with acetone-fixed first-generation merozoites of E. bovis; however, none of the MAbs reacted with live merozoites. Treatment of live sporozoites with EbS9 or EbS11 resulted in 79 and 73% decreases, respectively, in sporozoite penetration of MDBK cells. No significant differences in cell penetration occurred in MDBK cells inoculated with sporozoites that had been treated with the other three MAbs. Both EbS9 and EbS11 reacted in Western blots (immunoblots) of sporozoites with the same 20,000-relative-molecular-weight protein. The antigens against which these neutralizing MAbs react might be useful in immunizing against bovine coccidiosis.


Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Antiprotozoários/imunologia , Antígenos de Protozoários/imunologia , Antígenos de Superfície/imunologia , Eimeria/imunologia , Animais , Linhagem Celular , Células/parasitologia , Cães , Imunofluorescência , Imunoglobulina G/imunologia , Camundongos , Peso Molecular
20.
J Protozool ; 35(1): 119-22, 1988 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-2452879

RESUMO

Geographically diverse strains and clones of Tritrichomonas foetus have been examined with respect to their expression of a major surface antigen of approximately 150,000 relative molecular weight (Mr), designated the 150 Ag. Radioiodination and 35S-methionine labeling of T. foetus followed by immunoprecipitation with monoclonal antibodies (MAbs), separation of polypeptides by SDS-PAGE, and autoradiography or fluorography confirmed the parasite origin of the 150 Ag. The results of flow cytometry analysis employing a panel of MAbs against live T. foetus parasites revealed that from 5 to 84% of individuals in a given population of T. foetus expressed a particular epitope of the 150 Ag. All strains and clones were positive for surface expression of epitopes of this antigen. These results show that the 150 Ag is widely distributed in populations of T. foetus, confirm the surface location of this antigen, and suggest its importance as a target for protective immune responses.


Assuntos
Antígenos de Protozoários/análise , Tritrichomonas/imunologia , Animais , Antígenos de Superfície/análise , Eletroforese em Gel de Poliacrilamida , Epitopos/análise , Citometria de Fluxo , Imunoensaio
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA