RESUMO
UNLABELLED: Recent clinical data indicate that tumor hypoxia negatively affects the treatment outcome of both radiotherapy and surgery in various cancers, emphasizing the need for noninvasive detection of tumor hypoxia. Several studies have shown an increased uptake of FDG in hypoxic regions of xenografts, suggesting the use of PET with FDG as a potential technique. In this study, we examine the mechanism underlying the hypoxia-induced increase of FDG uptake in the human breast carcinoma cell line MCF7. METHODS: The uptake of 3H-FDG into MCF7 cells was determined after incubation under hypoxic (0% oxygen) or normoxic conditions, with or without redox agents, for varying time periods. In addition, the effects of the redox agents on the glucose transporter activity and the hexokinase activity were determined independently, and the effects of hypoxia on glucose transporter protein and hexokinase levels were assessed. RESULTS: A more than twofold increase (2.53 +/- 0.79; P < 0.005) in 3H-FDG uptake was observed under hypoxic conditions, but no changes in the cellular levels of glucose transporter proteins or hexokinase were observed. A reducing agent, dithiothreitol (DTT), also caused an increase in 3H-FDG uptake but failed to affect uptake under hypoxic conditions. This indicates that the mechanisms by which hypoxia and DTT affect 3H-FDG uptake might be the same. The oxidizing agent p-chloromercuribenzenesulfonic acid (pCMBS) had no effect on 3H-FDG uptake under normoxic conditions but counteracted the effect of hypoxia. DTT caused an increase in glucose transporter activity, whereas it had no effect on hexokinase activity. pCMBS had no effect on either glucose transporter activity or hexokinase activity. CONCLUSION: The hypoxia-induced increase in 3H-FDG uptake in MCF7 cells is the result, in part, of an increase in glucose transporter activity resulting from the modification (reduction) of thiol group(s) in the glucose transport protein(s). Modulation of hexokinase activity is probably not involved in the hypoxia-induced increase in 3H-FDG uptake in these cells.
Assuntos
Neoplasias da Mama/metabolismo , Fluordesoxiglucose F18/farmacocinética , 4-Cloromercuriobenzenossulfonato/farmacologia , Hipóxia Celular , Linhagem Celular , Ditiotreitol/farmacologia , Feminino , Hexoquinase/metabolismo , Humanos , Proteínas de Transporte de Monossacarídeos/metabolismo , Trítio , Células Tumorais CultivadasRESUMO
Heat shock prior, during, or immediately after ionizing radiation synergistically increases cell killing, a phenomenon termed hyperthermic radiosensitization. Recently, we have shown a constitutive DNA-binding factor in rodent cells that is inactivated by heat shock to be identical to Ku autoantigen. Ku, consisting of an Mr 70,000 (Ku70) and an Mr 86,000 (Ku80) subunit, is a heterodimeric nuclear protein and is the DNA-binding regulatory component of the mammalian DNA-dependent protein kinase DNA-PK. Recent genetic and biochemical studies indicate the involvement of Ku and DNA-PK in DNA double-strand break repair and V(D)J recombination. On the basis of these findings, we propose that heat-induced loss of the DNA-binding activity of Ku may lead to hyperthermic radiosensitization. To test this hypothesis, we examined and compared the DNA-binding activity of Ku, the DNA-PK kinase activity, and hyperthermic radiosensitization in rodent cells immediately after heat shock and during post-heat shock recovery at 37 degrees C. Our results show that the heat-induced loss of Ku-DNA binding activity correlates well with an increased radiosensitivity of the heat-shocked cells, and furthermore, the loss of synergistic interaction between heat and radiation parallels the recovery of the DNA-binding activity of Ku. On the other hand, the heat-induced decrease of DNA-PK activity did not correlate with hyperthermic radiosensitization. Our data, for the first time, provide evidence for a role of Ku protein in modulating the cellular response to combined treatments of heat shock and ionizing radiation.
Assuntos
Antígenos Nucleares , Autoantígenos/metabolismo , DNA Helicases , Proteínas de Ligação a DNA/metabolismo , Temperatura Alta , Proteínas Nucleares/metabolismo , Tolerância a Radiação , Animais , Células Cultivadas , DNA/metabolismo , Proteína Quinase Ativada por DNA , Autoantígeno Ku , Proteínas Serina-Treonina Quinases/metabolismo , RatosRESUMO
We recently have shown that mice deficient for the 86-kDa component (Ku80) of the DNA-dependent protein kinase exhibit growth retardation and a profound deficiency in V(D)J (variable, diversity, and joining) recombination. These defects may be related to abnormalities in DNA metabolism that arise from the inability of Ku80 mutant cells to process DNA double-strand breaks. To further characterize the role of Ku80 in DNA double-strand break repair, we have generated embryonic stem cells and pre-B cells and examined their response to ionizing radiation. Ku80(-/-) embryonic stem cells are more sensitive than controls to gamma-irradiation, and pre-B cells derived from Ku80 mutant mice display enhanced spontaneous and gamma-ray-induced apoptosis. We then determined the effects of ionizing radiation on the survival, growth, and lymphocyte development in Ku80-deficient mice. Ku80(-/-) mice display a hypersensitivity to gamma-irradiation, characterized by loss of hair pigmentation, severe injury to the gastrointestinal tract, and enhanced mortality. Exposure of newborn Ku80(-/-) mice to sublethal doses of ionizing radiation enhances their growth retardation and results in the induction of T cell-specific differentiation. However, unlike severe combined immunodeficient mice, radiation-induced T cell development in Ku80(-/-) mice is not accompanied by extensive thymocyte proliferation. The response of Ku80-deficient cell lines and mice to DNA-damaging agents provides important insights into the role of Ku80 in growth regulation, lymphocyte development, and DNA repair.
Assuntos
Antígenos Nucleares , Dano ao DNA , DNA Helicases , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Animais , Linfócitos B/citologia , Linfócitos B/imunologia , Linfócitos B/efeitos da radiação , Diferenciação Celular/efeitos da radiação , Linhagem Celular , Reparo do DNA , Feminino , Raios gama/efeitos adversos , Crescimento/genética , Crescimento/efeitos da radiação , Transtornos do Crescimento/etiologia , Transtornos do Crescimento/genética , Intestinos/patologia , Intestinos/efeitos da radiação , Autoantígeno Ku , Masculino , Camundongos , Camundongos Knockout , Camundongos SCID , Tolerância a Radiação , Linfócitos T/citologia , Linfócitos T/imunologia , Linfócitos T/efeitos da radiaçãoRESUMO
Previously, we proposed a dual control mechanism for the regulation of the heat shock response in mammalian cells: a positive control mediated by the heat shock transcription factor HSF1 and a negative control mediated by the constitutive heat shock element-binding factor (CHBF). To study the physiological role of CHBF in the regulation of heat shock response, we purified CHBF to apparent homogeneity and showed it to be identical to the Ku autoantigen, a heterodimer consisting of 70-kDa (Ku-70) and 86-kDa (Ku-80) polypeptides. To study further the functional significance of Ku/CHBF in the cellular response to heat shock, we established rodent cell lines that stably and constitutively overexpressed one or both subunits of the human Ku protein, and examined the thermal induction of hsp70 and other heat shock proteins in these Ku-overexpressing ing cells. We show that expression of the human Ku-70 and Ku-80 subunits jointly or of the Ku-70 subunit alone specifically inhibits heat-induced hsp70 expression. Conversely, expression of human Ku-80 alone does not have this effect. Thermal induction of other heat shock proteins in all of the Ku-overexpressing cell lines appears not to be significantly affected, nor is the state of phosphorylation or the DNA-binding ability of HSF1 affected. These findings support a model in which hsp70 expression is controlled by a second regulatory factor in addition to the positive activation of HSF1. The Ku protein, specifically the Ku-70 subunit, is involved in the regulation of hsp70 gene expression.
Assuntos
Antígenos Nucleares , Autoantígenos/metabolismo , DNA Helicases , Proteínas de Ligação a DNA/metabolismo , Proteínas de Choque Térmico HSP70/biossíntese , Proteínas Nucleares/metabolismo , Fatores de Transcrição/metabolismo , Animais , Sequência de Bases , Northern Blotting , Linhagem Celular , Sobrevivência Celular , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/isolamento & purificação , Expressão Gênica , Fatores de Transcrição de Choque Térmico , Temperatura Alta , Humanos , Cinética , Autoantígeno Ku , Substâncias Macromoleculares , Dados de Sequência Molecular , Proteínas Nucleares/química , Proteínas Nucleares/isolamento & purificação , Oligodesoxirribonucleotídeos , Fosforilação , RNA Mensageiro/biossíntese , Ratos , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Fatores de Tempo , TransfecçãoRESUMO
Thermotolerance (TT) induced by sodium arsenite (A-TT: 100 microM, 1 h, 37 degrees C) was compared to heat-induced thermotolerance (H-TT: 15 min, 44 degrees C) using HeLa S3 cells. All four pretreatments led to comparable levels of thermotolerance and also induced resistance to arsenite-, ethanol-, and diamide-induced toxicity (clonogenic ability). Stress-induced expression of the major heat shock proteins (hsp27, hsc70(p73), hsp70(p72), and hsp90) was generally highest in H-TT cells and lowest in A-TT cells. Interestingly, the four types of TT cells showed distinct differences in certain aspects of resistance against thermal protein damage. Thermal protein denaturation and aggregation determined in isolated cellular membrane fractions was found to be attenuated when they were isolated from H-TT and A-TT cells but not when isolated from E-TT and D-TT cells. The heat resistance in the proteins of the membrane fraction corresponded with elevated levels of hsp70(p72) associated with the isolated membrane fractions. In the nuclear fraction, only marginal (not significant) attenuation of the formation of protein aggregates (as determined by TX-100 (in)solubility) was observed. However, the postheat recovery from heat-induced protein aggregation in the nucleus was faster in H-TT, E-TT, and D-TT cells, but not in A-TT cells. Despite the fact that elevated levels of hsp27, hsp70(p73), and hsp70(p72) were found in the TX-100 insoluble nuclear fraction derived from all TT cells, no correlation was found with the degree of resistance in terms of the accelerated recovery from nuclear protein aggregation. The only correlation between accelerated recovery from nuclear protein aggregates was that with total cellular levels of hsp27. The data indicate that heat-induced loss of clonogenic ability may be a multitarget rather than a single target event. A threshold of damage may exist in cells after exposure to heat; multiple sets of proteins in (different compartments of) the cell need to be damaged before this threshold is exceeded and the cell dies. As a consequence, stabilization of only one of these sets of proteins is already sufficient to render cells thermotolerant at the clonogenic level.
Assuntos
Proteínas de Choque Térmico/fisiologia , Proteínas/química , Arsenitos/farmacologia , Diamida/farmacologia , Etanol/farmacologia , Células HeLa , Proteínas de Choque Térmico/química , Humanos , Conformação Proteica/efeitos dos fármacos , Desnaturação Proteica/efeitos dos fármacos , Compostos de Sódio/farmacologia , TemperaturaRESUMO
Analysis of the heat shock element (HSE)-binding proteins in extracts of rodent cells, during heat shock and their post-heat shock recovery, indicates that the regulation of heat shock response involves a constitutive HSE-binding factor (CHBF), in addition to the heat-inducible heat shock factor HSF1. We purified the CHBF to apparent homogeneity from HeLa cells using column chromatographic techniques including an HSE oligonucleotide affinity column. The purified CHBF consists of two polypeptides with apparent molecular masses of 70 and 86 kDa. Immunoblot and gel mobility shift analysis verify that CHBF is identical or closely related to the Ku autoantigen. The DNA binding characteristics of CHBF to double-stranded or single-stranded DNA are similar to that of Ku autoantigen. In gel mobility shift analysis using purified CHBF and recombinant human HSF1, CHBF competes with HSF1 for the binding of DNA sequences containing HSEs in vitro. Furthermore, when Rat-1 cells were co-transfected with human Ku expression vectors and the hsp70-promoter-driven luciferase reporter gene, thermal induction of luciferase is significantly suppressed relative to cells transfected with only the hsp70-luciferase construct. These data suggest a role of CHBF (or Ku protein) in the regulation of heat response in vivo.
Assuntos
Antígenos Nucleares , DNA Helicases , Proteínas de Ligação a DNA/metabolismo , Proteínas Nucleares/metabolismo , Animais , Anticorpos Monoclonais , Sequência de Bases , Sítios de Ligação , Ligação Competitiva , Linhagem Celular , Cromatografia de Afinidade , DNA/metabolismo , DNA de Cadeia Simples/metabolismo , Proteínas de Ligação a DNA/imunologia , Proteínas de Ligação a DNA/isolamento & purificação , Células HeLa , Temperatura Alta , Humanos , Autoantígeno Ku , Dados de Sequência Molecular , Peso Molecular , Proteínas Nucleares/imunologia , Oligodesoxirribonucleotídeos/metabolismo , Ratos , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Especificidade por Substrato , Transfecção , Raios UltravioletaRESUMO
Expression of the 70-kDa polypeptide of human Ku autoantigen in rat cells is shown to suppress specifically the induction of hsp70 upon heat shock. Thermal induction of other heat shock proteins is not significantly affected, nor is the state of phosphorylation or the DNA-binding ability of the heat shock transcription factor HSF1. These findings support a model in which hsp70 gene expression is controlled by a second regulatory factor in addition to the positive activator HSF1. The Ku autoantigen, or a protein closely related to it, is likely to be involved in the regulation of hsp70 expression.
Assuntos
Antígenos Nucleares , Autoantígenos/metabolismo , DNA Helicases , Proteínas de Ligação a DNA/metabolismo , Expressão Gênica , Proteínas de Choque Térmico HSP70/biossíntese , Proteínas Nucleares/metabolismo , Animais , Sequência de Bases , Sítios de Ligação , Western Blotting , Linhagem Celular , Proteínas de Ligação a DNA/biossíntese , Fibroblastos/citologia , Fibroblastos/metabolismo , Proteínas de Choque Térmico HSP70/antagonistas & inibidores , Fatores de Transcrição de Choque Térmico , Humanos , Cinética , Autoantígeno Ku , Substâncias Macromoleculares , Dados de Sequência Molecular , Proteínas Nucleares/biossíntese , Oligodesoxirribonucleotídeos , Ratos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/metabolismo , Fatores de Transcrição/metabolismo , TransfecçãoRESUMO
Heat, sodium-arsenite, and ethanol-induced thermotolerance are compared, especially with regard to the induced resistance of proteins of the particulate fraction (PF) against heat-induced denaturation. While all three agents induce thermotolerance as expressed as an enhanced survival after hyperthermic treatment, it is found that while heat and sodium-arsenite also induce resistance in the PF, this is not the case for ethanol. To explain these differences a hypothesis is postulated in which resistance is induced in those subcellular fractions/structures that are damaged by the agent used for the induction of thermotolerance. Furthermore, the effect of inhibition of protein synthesis by cycloheximide during the development of thermotolerance is investigated. It is found that while heat- and ethanol-induced thermotolerance (survival) are partly protein synthesis-independent, sodium-arsenite-induced thermotolerance (survival) is completely protein synthesis-dependent. Protein-synthesis-independent thermotolerance induced heat resistance in the proteins of the PF to the same extent as protein-synthesis-independent thermotolerance. To explain the differences in the ability of the agents to induce protein-synthesis-independent thermotolerance a hypothesis is postulated in which this ability depends on the mechanism by which this agent inhibits protein synthesis during the thermotolerance-inducing treatment. In this hypothesis the involvement of hsp in protein synthesis-independent thermotolerance is assumed.
Assuntos
Arsenitos , Temperatura Alta , Desnaturação Proteica , Compostos de Sódio , Arsênio/farmacologia , Sobrevivência Celular , Cicloeximida/farmacologia , Espectroscopia de Ressonância de Spin Eletrônica , Etanol/farmacologia , Células HeLa , Proteínas de Choque Térmico/biossíntese , Humanos , Biossíntese de Proteínas , Desnaturação Proteica/efeitos dos fármacosRESUMO
In this study we investigated the effect of heat on the proteins of the particulate fraction (PF) of HeLa S3 cells using electron spin resonance (ESR) and thermal gel analysis (TGA). ESR detects overall conformational changes in proteins, while TGA detects denaturation (aggregation due to formation of disulfide bonds) in specific proteins. For ESR measurements the -SH groups of the proteins were labelled with a maleimido bound spin label (4-maleimido-tempo). The sample was heated inside the ESR spectrometer at a rate of 1 degree C/min. ESR spectra were made every 2-3 degrees C between 20 degrees C and 70 degrees C. In the PF of untreated cells conformational changes in proteins were observed in three temperature stretches: between 38 and 44 degrees C (transition A, TA); between 47 and 53 degrees C (transition B, TB); and above 58 degrees C (transition C, TC). With TGA, using the same heating rate, we identified three proteins (55, 70, and 90 kD) which denatured during TB. No protein denaturation was observed during TA, while during TC denaturation of all remaining proteins in the PF occurred. When the ESR and TGA measurements were done with the PF of (heat-induced) thermotolerant cells, TA was unchanged while TB and TC started at higher temperatures. The temperature shift for the onset of these transitions correlated with the degree of thermotolerance that was induced in the cells. These results suggest that protection against heat-induced denaturation of proteins in the PF is involved in heat induced thermotolerance.
Assuntos
Temperatura Alta , Proteínas de Membrana/química , Desnaturação Proteica/fisiologia , Adaptação Fisiológica , Fracionamento Celular , Sobrevivência Celular , Espectroscopia de Ressonância de Spin Eletrônica , Células HeLa , HumanosRESUMO
The purpose of this study was to investigate possible involvement of poly(ADP-ribosyl)ation reactions in X-ray-induced cell killing, repair of potentially lethal damage (PLD), and formation and repair of radiation-induced DNA damage. As tools we used the inhibitors of poly(ADP-ribose)polymerase, 3-aminobenzamide (3AB), and 4-aminobenzamide (4AB). Both drugs inhibited PLD repair equally well but did not increase radiation-induced cell killing when cells were plated immediately after irradiation. 3AB affected repair of radiation-induced DNA damage, while 4AB had no effect. When 3AB was combined with aphidicolin (APC), it was found that the amount of DNA damage increased during the postirradiation incubation period. This means that the presence of 3AB stimulates the formation of DNA damage after X-irradiation. It is concluded that 3AB and 4AB sensitize HeLaS3 cells for radiation-induced cell killing by inhibiting repair of PLD. Because of the different effects of both inhibitors on repair of PLD and repair of radiation-induced DNA damage (a process known to be affected by inhibition of poly(ADP-ribosyl)ation), it is concluded that the observed inhibition of PLD repair is not caused by inhibition of poly(ADP-ribose)polymerase, and that the inhibitors affect repair of PLD and repair of DNA damage through independent mechanisms.
Assuntos
Benzamidas/farmacologia , Sobrevivência Celular/efeitos da radiação , Reparo do DNA/efeitos da radiação , Inibidores de Poli(ADP-Ribose) Polimerases , Ácido 4-Aminobenzoico/farmacologia , Afidicolina , Sobrevivência Celular/efeitos dos fármacos , DNA/efeitos dos fármacos , DNA/efeitos da radiação , Reparo do DNA/efeitos dos fármacos , Diterpenos/farmacologia , Células HeLa , Humanos , Estimulação Química , para-AminobenzoatosRESUMO
The purpose of this study was to investigate a possible involvement of poly(ADP-ribosyl)ation reactions in hyperthermic cell killing and hyperthermic DNA strand-break induction and repair in HeLa S3 cells. The inhibitors of poly(ADP-ribose) polymerase, 3-aminobenzamide (3AB) and 4-aminobenzamide (4AB), were used as tools in this study. Both inhibitors could sensitize the cells for hyperthermic cell killing equally well, although 3AB is known to be a more effective enzyme inhibitor. The heat sensitization at the level of cell killing could be reversed when the compounds were still present during a 4-h postincubation at 37 degrees C. More heat-induced DNA strand breaks were formed in the presence of 3AB and 4AB. Repair of strand breaks was inhibited during the postincubation at 37 degrees C. Thus the effect of 3AB and 4AB on DNA strand-break repair was different from the cited effect on cell survival. It is concluded that the sensitizing effect of 3AB and 4AB on hyperthermic cell killing is not caused by inhibition of poly(ADP-ribose) polymerase and is also not related to repair of DNA strand breaks.
Assuntos
Temperatura Alta , Inibidores de Poli(ADP-Ribose) Polimerases , Ácido 4-Aminobenzoico/farmacologia , Adaptação Fisiológica/efeitos dos fármacos , Aminobenzoatos/farmacologia , Benzamidas/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Dano ao DNA , Reparo do DNA/efeitos dos fármacos , DNA de Neoplasias/efeitos dos fármacos , Células HeLa , Humanos , meta-Aminobenzoatos , para-AminobenzoatosRESUMO
Three different cell lines, Ehrlich ascites tumour (EAT) cells, HeLa S3 cells and LM mouse fibroblasts, were used to investigate whether or not the extent of heat killing (44 degrees C) and heat radio-sensitization (44 degrees C before 0-6 Gy X-irradiation) are related. Although HeLa cells were the most heat-resistant cell line and showed the least heat radiosensitization, we found that the most heat-sensitive EAT cells (D0, EAT = 8.0 min; D0, LM = 10.0 min; D0, HeLa = 12.5 min) showed less radiosensitization than the more heat-resistant LM fibroblasts (TERHeLa less than TEREAT less than TERLM). Therefore, it is concluded that the routes leading to heat-induced cell death are not identical to those determining heat radiosensitization. Furthermore the inactivation of DNA polymerase alpha and beta activities by heat seemed not to correlate with heat survival alone but showed a positive relationship to heat radiosensitization. The possibility of these enzymes being a determinant in heat radiosensitization is discussed.
Assuntos
Sobrevivência Celular/efeitos da radiação , Temperatura Alta , Aclimatação , Animais , Linhagem Celular , Humanos , Camundongos , Tolerância a RadiaçãoRESUMO
Possible relations between hyperthermic inactivation of alpha and beta DNA polymerase activity and hyperthermic cell killing or hyperthermic radiosensitization were investigated. Ehrlich Ascites Tumor (EAT) cells and HeLa S3 cells were treated with fractionated doses of hyperthermia. The heating schedules were chosen such that the initial heat treatment resulted in either thermotolerance or thermosensitization (step-down heating) for the second heat treatment. The results show that for DNA polymerase activity and heat radiosensitization (cell survival) no thermotolerance or thermosensitization is observed. Thus hyperthermic cell killing and DNA polymerase activity are not correlated. The correlation of hyperthermic radiosensitization and DNA polymerase activity was substantially less than observed in previous experiments with normotolerant and thermotolerant HeLa S3 cells. We conclude that alpha and beta DNA polymerase inactivation is not always the critical cellular process responsible for hyperthermic cell killing or hyperthermic radiosensitization. Other possible cellular systems that might determine these processes are discussed.