Disentangling Gender and Relative Age Effects in Women's and Girls' Rugby Union.

Relative age effects (RAEs) within sports refer to the overrepresentation of athletes born earlier in the selection year and the underrepresentation of those born later in the selection year. Research examining RAEs in women's and girls' rugby union remains limited in comparison to the male literature, whilst the impacts of RAEs on the youth-senior transition are yet to be explored in a female sport context. As such, the purpose of this study was to examine RAEs during entry into the women's and girls' premiership and international rugby union pathways in England, as well as to compare them to their respective senior cohort (n = 1367): (a) U18 England Rugby Centre of Excellence Player (n = 325) vs. Senior Premiership Player (n = 868), and (b) U18 England Player (n = 49) vs. Senior England Player (n = 125). Chi-square (χ2) analyses compared birth quarter (BQ) distributions against expected distributions. The findings revealed no significant difference in BQ distributions at either youth or senior levels, as well as no significant differences in the BQ distributions of those who were likely to transition from youth to senior levels (all p > 0.05). Importantly, though, descriptive statistics showed a skewed birthdate distribution in both U18 England Rugby Centre of Excellence Player (BQ1 = 30% vs. BQ4 = 20%) and U18 England Player cohorts (BQ1 = 33% vs. BQ4 = 18%). We highlight the gender-specific mechanisms that potentially explain the variations between male and female RAEs in rugby union, including developmental differences, sport popularity, and sociocultural norms. We also warn against a 'copy and paste' template from the male provision to ensure the recent growth of female rugby union does not fall victim to the same RAEs in the future.

The relative age effect in male and female English age-grade rugby union: Exploring the gender-specific mechanisms that underpin participation.

The relative age effect (RAE) is a phenomenon that represents how young athletes who are born early in the selection year are often overrepresented within youth sport settings. The contact nature of rugby union may further magnify the physiological advantages of those athletes who are chronologically older. Thus, the purpose of this study was to examine the RAE within English age-grade rugby union. Male (n = 228,206) and female (n = 23,563) English age-grade rugby union participants were allocated into their 12-month annual age-category (under-7 to under-18). Data was analysed using a chi-square goodness-of-fit test to compare the observed and expected distributions. Significant differences were revealed in all male (p < 0.001) and nine out of twelve female (p < 0.05) annual-age categories. From a male perspective, a higher relative difference became present at under-14 onwards, suggesting that there may be further implications due to the onset of puberty and the introduction of 15-a-side competition. Further female analysis revealed that there was a within-2-year effect in their 24-month age groups (under-13 and under-15). Interestingly, there was an inverse within-3-year effect (i.e., an overrepresentation of younger players) within the female 36-month age group (under-18). The key findings indicate a RAE has become ingrained in English age-grade rugby union, as well as outlining important gender-specific considerations.

Generation of Monoubiquitin and K63-Linked Polyubiquitin Chains for Protein Interaction Studies.

Ubiquitylation is a posttranslational modification that utilizes protein-protein binding interactions to regulate cellular processes. In ubiquitin signaling, a vast array of mono- and polyubiquitin modifications to substrate proteins are recognized by a diverse group of ubiquitin-binding proteins. Identifying ubiquitin-binding proteins and characterizing their binding properties is necessary for understanding the structural basis of ubiquitin signaling. This chapter provides a means of studying ubiquitin-binding interactions in vitro by describing how to generate monoubiquitin and K63-linked polyubiquitin chains and perform pull-down assays with ubiquitin-binding proteins, which is of particular relevance for DNA damage and other signaling pathways.

The ASCC2 CUE domain in the ALKBH3-ASCC DNA repair complex recognizes adjacent ubiquitins in K63-linked polyubiquitin.

Alkylation of DNA and RNA is a potentially toxic lesion that can result in mutations and even cell death. In response to alkylation damage, K63-linked polyubiquitin chains are assembled that localize the Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3-Activating Signal Cointegrator 1 Complex Subunit (ASCC) repair complex to damage sites in the nucleus. The protein ASCC2, a subunit of the ASCC complex, selectively binds K63-linked polyubiquitin chains via its coupling of ubiquitin conjugation to ER degradation (CUE) domain. The basis for polyubiquitin-binding specificity was unclear, because CUE domains in other proteins typically bind a single ubiquitin and do not discriminate among different polyubiquitin linkage types. We report here that the ASCC2 CUE domain selectively binds K63-linked diubiquitin by contacting both the distal and proximal ubiquitin. The ASCC2 CUE domain binds the distal ubiquitin in a manner similar to that reported for other CUE domains bound to a single ubiquitin, whereas the contacts with the proximal ubiquitin are unique to ASCC2. Residues in the N-terminal portion of the ASCC2 α1 helix contribute to the binding interaction with the proximal ubiquitin of K63-linked diubiquitin. Mutation of residues within the N-terminal portion of the ASCC2 α1 helix decreases ASCC2 recruitment in response to DNA alkylation, supporting the functional significance of these interactions during the alkylation damage response. Our study reveals the versatility of CUE domains in ubiquitin recognition.

Talent Identification and Relative Age Effects in English Male Rugby Union Pathways: From Entry to Expertise.

A common practice in youth rugby union is to group players based on (bi)annual age with fixed cut-off dates. The overrepresentation of players born at the start of the cut-off date and the underrepresentation of players born toward the end of the cut-off date are termed relative age effects (RAEs). The aim of this study was to examine RAEs during entry into professional and international rugby union pathways in England, as well as comparing them to their respective senior cohort: U15 Regional Academy Player (n = 1,114) vs. Senior Professional Player (n = 281) and U16-23 England Academy Player (n = 849) vs. Senior International Player (n = 48). Chi-square (χ2) analysis compared birth quarter (BQ) distributions against expected distributions. Odds ratios and 95% confidence intervals compared the likelihood of a BQ being selected. Findings revealed a significant overrepresentation of relatively older players compared with their relatively younger peers within both youth cohorts (P < 0.001; BQ1 = 42.5% vs. BQ4 = 9.6%; BQ1 = 36.5% vs. BQ4 = 15.2%). In comparison, there was no significant difference in the BQ distributions within both senior cohorts. Further, BQ4s were 3.86 and 3.9 times more likely to achieve senior professional and international levels than BQ1s and BQ2s, respectively. It is suggested that relatively younger players may have a greater likelihood of achieving expertise following entry into a rugby union talent pathway due to benefitting from more competitive play against relatively older counterparts during their development (e.g., reversal effects; the underdog hypothesis). Moreover, possible solutions (e.g., age and anthropometric banding; playing-up and playing-down) are discussed to encourage practitioners and policy makers to create the most appropriate learning environment for every player.

The impact of rule modifications on player behaviour in a talent identification and development environment: A case study of the Rugby Football Union's Wellington Academy Rugby Festival.

Research has suggested that competition within talent identification and development systems should be modified from the adult format of the sport to meet the developmental needs of those participating. Yet limited research has evaluated the success of game changes, particularly the effectiveness of modifying the rules of a game to purposefully engineer changes in player behaviour. The purpose of this study was to monitor the impact of rule modifications on player behaviour within a talent identification and development system in rugby union. Performance indicators (ball in play, pass, offload, kick) were collected during full length (70 min) and shortened durations (30-42 min) of competitive matches played during a weeklong under sixteen rugby union festival in 2016 and after rule modifications were introduced in 2017-2019. The findings indicate that rule modifications had the prescribed impact on player actions, particularly in the shortened duration formats of the game. Therefore, rule modifications provide talent developers a tool to manipulate player behaviour, in this case skill attempts, within full-sided competitive matches.

Susceptibility to a multisensory speech illusion in older persons is driven by perceptual processes.

Recent studies suggest that multisensory integration is enhanced in older adults but it is not known whether this enhancement is solely driven by perceptual processes or affected by cognitive processes. Using the "McGurk illusion," in Experiment 1 we found that audio-visual integration of incongruent audio-visual words was higher in older adults than in younger adults, although the recognition of either audio- or visual-only presented words was the same across groups. In Experiment 2 we tested recall of sentences within which an incongruent audio-visual speech word was embedded. The overall semantic meaning of the sentence was compatible with either one of the unisensory components of the target word and/or with the illusory percept. Older participants recalled more illusory audio-visual words in sentences than younger adults, however, there was no differential effect of word compatibility on recall for the two groups. Our findings suggest that the relatively high susceptibility to the audio-visual speech illusion in older participants is due more to perceptual than cognitive processing.

Factors associated with perceived health in older adult Irish population.

OBJECTIVES: Perceived health status or self-reported health declines with age and is associated with increased morbidity, mortality, poor mental health and functional outcomes and health care utilisation. The aim of this study was to investigate the relationship between a range of biopsychosocial measures and self-reported health in a sample of community-dwelling older people living in Dublin. METHOD: A total of 492 participants with a mean age of 72.5 (SD = 7.34) were assessed at the Technology Research for Independent Living (TRIL) clinic, a comprehensive geriatric assessment facility in St James's Hospital, Dublin, using standardised measures of medical morbidity, personality, functional status, depression, anxiety, perceived stress, cognition, loneliness and social support. The relationship between self-rated health and health measures was analysed using bivariate correlations and backward multiple regression. Indirect pathways of effects on perceived health were evaluated using bootstrapping mediation analysis. RESULTS: Self-rated health was best predicted by instrumental activities of daily living (IADL), Charlson Co-morbidity Index (CCI), extraversion and perceived stress. The effects of cognition and social support from friends on self-rated health were found to be mediated by elements of these identified factors. CONCLUSION: Self-rated health is not simply the absence of physical illness but is also strongly influenced by the degree of functional impairment, personality factors and the level of stress experienced by the older person.

The effect of combined sensory and semantic components on audio-visual speech perception in older adults.

Previous studies have found that perception in older people benefits from multisensory over unisensory information. As normal speech recognition is affected by both the auditory input and the visual lip movements of the speaker, we investigated the efficiency of audio and visual integration in an older population by manipulating the relative reliability of the auditory and visual information in speech. We also investigated the role of the semantic context of the sentence to assess whether audio-visual integration is affected by top-down semantic processing. We presented participants with audio-visual sentences in which the visual component was either blurred or not blurred. We found that there was a greater cost in recall performance for semantically meaningless speech in the audio-visual 'blur' compared to audio-visual 'no blur' condition and this effect was specific to the older group. Our findings have implications for understanding how aging affects efficient multisensory integration for the perception of speech and suggests that multisensory inputs may benefit speech perception in older adults when the semantic content of the speech is unpredictable.

Is inefficient multisensory processing associated with falls in older people?

Although falling is a significant problem for older persons, little is understood about its underlying causes. Spatial cognition and balance maintenance rely on the efficient integration of information across the main senses. We investigated general multisensory efficiency in older persons with a history of falls compared to age- and sensory acuity-matched controls and younger adults using a sound-induced flash illusion. Older fallers were as susceptible to the illusion as age-matched, non-fallers or younger adults at a short delay of 70 ms between the auditory and visual stimuli. Both older adult groups were more susceptible to the illusion at longer SOAs than younger adults. However, with increasing delays between the visual and auditory stimuli, older fallers did not show a decline in the frequency at which the illusion was experienced even with delays of up to 270 ms. We argue that this relatively higher susceptibility to the illusion reflects inefficient audio-visual processing in the central nervous system and has important implications for the diagnosis and rehabilitation of falling in older persons.