Spatial distribution and fixed-precision sequential sampling plans for Popillia japonica (Coleoptera: Scarabaeidae) adults in primocane raspberry: influence of foliar insecticides.

The Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), an invasive species from northern Japan, was first detected in Minnesota in 1968. According to fruit growers and the Minnesota Department of Agriculture, population size and feeding damage has been an increasing concern since 2010. Based on trap-catch data, populations have recently exceeded 4,000 beetles/trap/week during July-August near raspberry fields, and can increase by an order of magnitude within 7-10 days. The primary goals of this study were to assess the spatial distribution of P. japonica adults in raspberry, and to develop and validate a practical fixed-precision sequential sampling plan for grower use. Taylor's Power Law (TPL) regression was used to characterize the beetle's spatial pattern in research plots and commercial fields, either with or without insecticide applications. We then used Green's plan to develop an enumerative sequential sampling plan to estimate P. japonica density in primocane raspberry. Beetle population data were collected at two locations in southern Minnesota, including the Rosemount Research and Outreach Center, and a commercial field near Forest Lake. The TPL results, via slope comparisons, indicated no significant differences in P. japonica spatial pattern between insecticide treated plots versus untreated plots, or among 4 different insecticides (P>0.05). Utilizing all spatial pattern data, we characterized the distribution of P. japonica beetles to be highly aggregated in raspberry, with TPL slopes ranging from b = 1.38 to 1.55; all slopes were found to be >1.0. Although the slopes were not significantly different, we accounted for variability in spatial pattern by using 33 independent data sets, and the Resampling for Validation of Sampling Plans (RVSP) model to validate a sampling plan with a final average precision level of 0.25 (SEM/mean), recommended for integrated pest management (IPM) purposes. The final sampling plan required an average sample number of only 15, 1-m-row samples, while providing high relative net precision (RNP), and thus a cost-effective, efficient sample plan for growers.

Extended Sentinel Monitoring of Helicoverpa zea Resistance to Cry and Vip3Aa Toxins in Bt Sweet Corn: Assessing Changes in Phenotypic and Allele Frequencies of Resistance.

Transgenic corn and cotton that produce Cry and Vip3Aa toxins derived from Bacillus thuringiensis (Bt) are widely planted in the United States to control lepidopteran pests. The sustainability of these Bt crops is threatened because the corn earworm/bollworm, Helicoverpa zea (Boddie), is evolving a resistance to these toxins. Using Bt sweet corn as a sentinel plant to monitor the evolution of resistance, collaborators established 146 trials in twenty-five states and five Canadian provinces during 2020-2022. The study evaluated overall changes in the phenotypic frequency of resistance (the ratio of larval densities in Bt ears relative to densities in non-Bt ears) in H. zea populations and the range of resistance allele frequencies for Cry1Ab and Vip3Aa. The results revealed a widespread resistance to Cry1Ab, Cry2Ab2, and Cry1A.105 Cry toxins, with higher numbers of larvae surviving in Bt ears than in non-Bt ears at many trial locations. Depending on assumptions about the inheritance of resistance, allele frequencies for Cry1Ab ranged from 0.465 (dominant resistance) to 0.995 (recessive resistance). Although Vip3Aa provided high control efficacy against H. zea, the results show a notable increase in ear damage and a number of surviving older larvae, particularly at southern locations. Assuming recessive resistance, the estimated resistance allele frequencies for Vip3Aa ranged from 0.115 in the Gulf states to 0.032 at more northern locations. These findings indicate that better resistance management practices are urgently needed to sustain efficacy the of corn and cotton that produce Vip3Aa.

Exclusion and Repulsion of Popillia japonica (Coleoptera: Scarabaeidae) Using Selected Coverings on High Tunnel Structures for Primocane Red Raspberry.

In temperate climates, there has been an increasing interest by fruit growers to implement the use of high tunnels, using a variety of coverings, to extend the season for fruit production. High tunnels also provide an opportunity to enhance insect pest management, via physical exclusion, and thus support reductions in insecticide use. Due to increasing pest pressure by the Japanese beetle, Popillia japonica Newman, in Midwest U.S. raspberry, a 3-year study (2017−2019) was conducted to evaluate the efficacy of selected high tunnel coverings to suppress adult beetle populations and minimize adult feeding injury. During each year of the study, P. japonica adult beetles were significantly reduced under poly-based coverings, with the ends open, and when a fine, nylon-mesh was used to cover the ends (p < 0.05). The poly-based covering also resulted in moderately higher ambient temperatures, which may have influenced beetle movement, including a "repellency effect" that encouraged beetles to exit the high tunnel structures. Although P. japonica adults are known to feed on raspberry flower clusters, including fruit, the majority (73−92%) of beetle feeding in this study was observed on the foliage. The impact of high tunnels on P. japonica are discussed within the context of developing sustainable Integrated Pest Management (IPM) programs for raspberry production.

Pest population dynamics are related to a continental overwintering gradient.

Overwintering success is an important determinant of arthropod populations that must be considered as climate change continues to influence the spatiotemporal population dynamics of agricultural pests. Using a long-term monitoring database and biologically relevant overwintering zones, we modeled the annual and seasonal population dynamics of a common pest, Helicoverpa zea (Boddie), based on three overwintering suitability zones throughout North America using four decades of soil temperatures: the southern range (able to persist through winter), transitional zone (uncertain overwintering survivorship), and northern limits (unable to survive winter). Our model indicates H. zea population dynamics are hierarchically structured with continental-level effects that are partitioned into three geographic zones. Seasonal populations were initially detected in the southern range, where they experienced multiple large population peaks. All three zones experienced a final peak between late July (southern range) and mid-August to mid-September (transitional zone and northern limits). The southern range expanded by 3% since 1981 and is projected to increase by twofold by 2099 but the areas of other zones are expected to decrease in the future. These changes suggest larger populations may persist at higher latitudes in the future due to reduced low-temperature lethal events during winter. Because H. zea is a highly migratory pest, predicting when populations accumulate in one region can inform synchronous or lagged population development in other regions. We show the value of combining long-term datasets, remotely sensed data, and laboratory findings to inform forecasting of insect pests.

HUGE pipeline to measure temporal genetic variation in Drosophila suzukii populations for genetic biocontrol applications.

Understanding the fine-scale genome sequence diversity that exists within natural populations is important for developing models of species migration, temporal stability, and range expansion. For invasive species, agricultural pests, and disease vectors, sequence diversity at specific loci in the genome can impact the efficacy of next-generation genetic biocontrol strategies. Here we describe a pipeline for haplotype-resolution genetic variant discovery and quantification from thousands of Spotted Wing Drosophila (Drosophila suzukii, SWD) isolated at two field sites in the North-Central United States (Minnesota) across two seasons. We observed highly similar single nucleotide polymorphism (SNP) frequencies at each genomic location at each field site and year. This supports the hypotheses that SWD overwinters in Minnesota, is annually populated by the same source populations or a combination of both theories. Also, the stable genetic structure of SWD populations allows for the rational design of genetic biocontrol technologies for population suppression.

Impact of Adult Popillia japonica (Coleoptera: Scarabaeidae) Foliar Feeding Injury on Fruit Yield and Quality of a Temperate, Cold-Hardy Wine Grape, 'Frontenac'.

Popillia japonica (Newman), is a highly polyphagous, invasive species, first recorded in the U.S. in 1916, and detected in Minnesota in the late 1960s. Historically, research on this pest in the Midwest U.S. has focused primarily on ornamental and turf crops, with little attention placed on adult feeding damage to fruit crops. Recently, wine grape producers in the region noted substantial increases in defoliation from P. japonica feeding, confirming concerns for this perennial high value crop. To address these concerns, studies were conducted during the summers of 2020-2021 to understand the impact of P. japonica foliar feeding on the quality and yield of wine grapes. Trials utilized vines of the wine grape variety, 'Frontenac.' In addition to open plots, whole vines were caged within fine mesh netting and infested with P. japonica at 0, 25, 50, and 100 beetles per meter-row of vine. Beetles used for infestations were collected from natural field populations of P. japonica and left to feed until grapes were ready for harvest. During harvest, data collection included leaf samples for obtaining average percent defoliation, cluster weights, and berry subsamples for soluble solid content, pH, titratable acidity, and phenolic compound measurements. Results from these studies demonstrated that as beetle population density and defoliation per m-row increases, at-harvest measurements of quality parameters are significantly and negatively affected (P < 0.05) when compared with uninfested vines. The negative impacts to fruit quality exhibited in these studies will be important in the development of future management strategies for P. japonica in 'Frontenac.'

Novel real-time PCR based assays for differentiating fall armyworm strains using four single nucleotide polymorphisms.

The fall armyworm, Spodoptera frugiperda, is a polyphagous global pest with a preference for gramineous crops such as corn, sorghum and pasture grasses. This species is comprised of two morphologically identical but genetically distinct host strains known as the corn and rice strains, which can complicate pest management approaches. Two molecular markers are commonly used to differentiate between strains, however, discordance between these markers can lead to inconclusive strain identification. Here, we used double digest restriction site associated DNA sequencing to identify diagnostic single nucleotide polymorphisms (SNPs) with alleles unique to each strain. We then used these strain-specific SNPs to develop four real-time PCR based TaqMan assays to rapidly and reliably differentiate between strains and interstrain hybrids. These assays provide a new tool for differentiating between strains in field-collected samples, facilitating future studies on strain population dynamics and interstrain hybridization rates. Understanding the basic ecology of S. frugiperda strains is necessary to inform future management strategies.

Presence-Absence Sampling Plans for Stink Bugs (Hemiptera: Pentatomidae) in the Midwest Region of the United States.

Stink bugs represent an increasing risk to soybean production in the Midwest region of the United States. The current sampling protocol for stink bugs in this region is tailored for population density estimation and thus is more relevant to research purposes. A practical decision-making framework with more efficient sampling effort for management of herbivorous stink bugs is needed. Therefore, a binomial sequential sampling plan was developed for herbivorous stink bugs in the Midwest region. A total of 146 soybean fields were sampled across 11 states using sweep nets in 2016, 2017, and 2018. The binomial sequential sampling plans were developed using combinations of five tally thresholds at two proportion infested action thresholds to identify those that provided the best sampling outcomes. Final assessment of the operating characteristic curves for each plan indicated that a tally threshold of 3 stink bugs per 25 sweeps, and proportion infested action thresholds of 0.75 and 0.95 corresponding to the action thresholds of 5 and 10 stink bugs per 25 sweeps, provided the optimal balance between highest probability of correct decisions (≥ 99%) and lowest probability of incorrect decisions (≤ 1%). In addition, the average sample size for both plans (18 and 12 sets of 25 sweeps, respectively) was lower than that for the other proposed plans. The binomial sequential sampling plan can reduce the number of sample units required to achieve a management decision, which is important because it can potentially reduce risk/cost of management for stink bugs in soybean in this region.

Season-Long Monitoring of the Brown Marmorated Stink Bug (Hemiptera: Pentatomidae) Throughout the United States Using Commercially Available Traps and Lures.

Reliable monitoring of the invasive Halyomorpha halys abundance, phenology and geographic distribution is critical for its management. Halyomorpha halys adult and nymphal captures on clear sticky traps and in black pyramid traps were compared in 18 states across the Great Lakes, Mid-Atlantic, Southeast, Pacific Northwest and Western regions of the United States. Traps were baited with commercial lures containing the H. halys pheromone and synergist, and deployed at field sites bordering agricultural or urban locations with H. halys host plants. Nymphal and adult captures in pyramid traps were greater than those on sticky traps, but captures were positively correlated between the two trap types within each region and during the early-, mid- and late season across all sites. Sites were further classified as having a low, moderate or high relative H. halys density and again showed positive correlations between captures for the two trap types for nymphs and adults. Among regions, the greatest adult captures were recorded in the Southeast and Mid-Atlantic on pyramid and sticky traps, respectively, with lowest captures recorded in the West. Nymphal captures, while lower than adult captures, were greatest in the Southeast and lowest in the West. Nymphal and adult captures were, generally, greatest during July-August and September-October, respectively. Trapping data were compared with available phenological models showing comparable population peaks at most locations. Results demonstrated that sticky traps offer a simpler alternative to pyramid traps, but both can be reliable tools to monitor H. halys in different geographical locations with varying population densities throughout the season.

Risk of Cold-Hardy Apple Cultivars for Injury From the Brown Marmorated Stink Bug (Hemiptera: Pentatomidae).

An invasive species, the brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), has emerged as a serious pest of orchard crops in the United States with the highest economic losses to date in Mid-Atlantic apple, Malus domestica Borkh. (Rosales: Rosaceae). If populations continue to grow and spread in the Midwest, H. halys has the potential to become a significant apple pest in the region. The purpose of this study was to assess the risk for injury from H. halys to several popular cold-hardy apple cultivars (Haralson, Honeycrisp, and Zestar!) grown in the Midwestern United States utilizing both field no-choice tests and laboratory choice tests at multiple timings. Results from the field no-choice tests revealed a greater risk for Honeycrisp from H. halys injury compared with Zestar! in mid-August. Results from the laboratory choice tests revealed a greater risk for injury by H. halys for Honeycrisp compared with Zestar! at all timings tested and a greater risk for Haralson compared with Honeycrisp at a later timing. These results echo previous findings in that relative maturity of the fruit seems to play a role in determining the risk of an apple cultivar to H. halys injury. These results also serve as the first assessment of the potential impact of H. halys on different cold-hardy apple cultivars, which will help guide growers in cultivar selection and identifying which apple cultivars should be prioritized for scouting and management efforts.

Evaluation of Exclusion Netting for Spotted-Wing Drosophila (Diptera: Drosophilidae) Management in Minnesota Wine Grapes.

Spotted-wing drosophila, Drosophila suzukii (Matsumura), an economically damaging invasive species of numerous fruit crops, was first detected in Minnesota in 2012. High fecundity, and short generation times facilitated a rapid rise in the global pest status of D. suzukii, particularly in North America and Europe. To date, the majority of crop injury research has focused on fruit crops such as blueberries, raspberries, and cherries. However, little is known regarding the impact of D. suzukii on the wine grape industry in the upper Midwest region of the United States. Field trials were conducted in Minnesota during the summers of 2017-2018 to examine season-long phenology of D. suzukii in wine grape vineyards and wineries, and to assess the efficacy of exclusion netting for control of D. suzukii. Four treatments were evaluated, 1) open plot check (control), 2) open plot treated with an insecticide, 3) exclusion netting, and 4) exclusion netting, with artificial infestations of D. suzukii adults. Exclusion netting was applied at véraison and removed at harvest. On each sample date, 20 berries (10 intact and 10 injured) were collected from each plot for dissection. The number of larvae and adults were recorded for each berry to determine infestation levels. As shown by mean larval infestations and injured berries across treatments, exclusion netting provided a significant reduction in the level of D. suzukii infested berries when compared with the untreated check. These results indicate that exclusion netting could provide an effective alternative management strategy for D. suzukii in wine grapes.

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar.

Elemental data are commonly used to infer plant quality as a resource to herbivores. However, the ubiquity of carbon in biomolecules, the presence of nitrogen-containing plant defensive compounds, and variation in species-specific correlations between nitrogen and plant protein content all limit the accuracy of these inferences. Additionally, research focused on plant and/or herbivore physiology require a level of accuracy that is not achieved using generalized correlations. The methods presented here offer researchers a clear and rapid protocol for directly measuring plant soluble proteins and digestible carbohydrates, the two plant macronutrients most closely tied to animal physiological performance. The protocols combine well characterized colorimetric assays with optimized plant-specific digestion steps to provide precise and reproducible results. Our analyses of different sweet corn tissues show that these assays have the sensitivity to detect variation in plant soluble protein and digestible carbohydrate content across multiple spatial scales. These include between-plant differences across growing regions and plant species or varieties, as well as within-plant differences in tissue type and even positional differences within the same tissue. Combining soluble protein and digestible carbohydrate content with elemental data also has the potential to provide new opportunities in plant biology to connect plant mineral nutrition with plant physiological processes. These analyses also help generate the soluble protein and digestible carbohydrate data needed to study nutritional ecology, plant-herbivore interactions and food-web dynamics, which will in turn enhance physiology and ecological research.

Effects of Diapause on Halyomorpha halys (Hemiptera: Pentatomidae) Cold Tolerance.

Diapause and cold tolerance can profoundly affect the distribution and activity of temperate insects. Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), an alien invasive species from Asia, enters a winter dormancy in response to environmental cues. We investigated the nature of this dormancy and its effects on H. halys cold tolerance, as measured by supercooling points, lower lethal temperatures, and overwintering field mortality. Dormancy was induced by rearing individuals in the laboratory or under field conditions. We confirmed H. halys dormancy to be a state of diapause and not quiescence, and the life stage sensitive to diapause-inducing cues is between the second and fifth instar. In the laboratory, supercooling points of diapausing adults reached significantly lower temperatures than nondiapausing adults, but only when given enough time after imaginal ecdysis. Supercooling points of diapausing adults in overwintering microhabitats also decreased over time. Diapause increased adult survival after acute cold exposure in the laboratory and prolonged cold exposure in the field. Following diapause induction in the laboratory, changes to temperature and photoperiod had no significant effect on lower lethal temperatures and changes to photoperiod had no effect on supercooling points. Additionally, induction of diapause in the laboratory did not result in significantly different cold tolerance than natural field induction of diapause. This work demonstrates that H. halys diapause confers greater cold tolerance than a nondiapausing state and likely improves the probability of successful overwintering in some temperate climates. Hence, knowledge of diapause status could be used to refine forecasts of H. halys overwintering field mortality.

Bt Maize Seed Mixtures for Helicoverpa zea (Lepidoptera: Noctuidae): Larval Movement, Development, and Survival on Non-transgenic Maize.

In 2012 and 2013, field trials were conducted near Rosemount, MN, to assess the movement and development of Helicoverpa zea (Boddie) larvae on non-Bt refuge corn plants within a seed mixture of non-Bt and Bt corn. The Bt corn hybrid expressed three Bt toxins-Cry1Ab, Cry1F, and Vip3A. As the use of seed mixtures for insect resistance management (IRM) continues to be implemented, it is necessary to further characterize how this IRM approach impacts resistance development in ear-feeding Lepidopteran pests. The potential for Bt pollen movement and cross pollination of the non-Bt ears in a seed mixture may lead to Bt toxin exposure to larvae developing on those refuge ears. Larval movement and development by H. zea, feeding on non-Bt refuge plants adjacent to either transgenic Bt or non-Bt plants, were measured to investigate the potential for unintended Bt exposure. Non-Bt plants were infested with H. zea eggs and subplots were destructively sampled twice per week within each treatment to assess larval development, location, and kernel injury. Results indicate that H. zea larval movement between plants is relatively low, ranging from 2-16% of larvae, and occurs mainly after reaching the second instar. Refuge plants in seed mixtures did not produce equivalent numbers of H. zea larvae, kernel injury, and larval development differed as compared with a pure stand of non-Bt plants. This suggests that there may be costs to larvae developing on refuge plants within seed mixtures and additional studies are warranted to define potential impacts.

Bt pollen dispersal and Bt kernel mosaics: integrity of non-Bt refugia for lepidopteran resistance management in maize.

Field trials were conducted at Rosemount, MN in 2009 and 2010, to measure pollen movement from Bt corn to adjacent blocks of non-Bt refuge corn. As the use of Bt corn hybrids continues to increase in the United States, and new insect resistance management (IRM) plans are implemented, it is necessary to measure the efficacy of these IRM plans. In Minnesota, the primary lepidopteran pests of corn include the European corn borer, Ostrinia nubilalis (Hübner) and corn earworm, Helicoverpa zea (Boddie). The primary IRM plan in transgenic corn is the use of hybrids expressing a high dose of insecticidal proteins and an insect refuge containing hybrids not expressing insecticidal proteins that produce susceptible insects. Wind-assisted pollen movement in corn occurs readily, and is the primary method of pollination for corn. The combination of pollen movement and viability determines the potential for cross pollination of refuge corn. In 2009 and 2010, cross pollination occurred with the highest frequency on the north and east sides of Bt corn fields, but was found at some level in all directions. Highest levels of cross pollination (75%) were found within the first four rows (3 m) of non-Bt corn adjacent to Bt corn, and in general decreasing levels of cross pollination were found the further the non-Bt corn was planted from the Bt corn. A mosaic of Bt cross-pollinated kernels was found throughout the ear, but in both years the ear tip had the highest percentage of cross-pollinated kernels; this pattern may be linked to the synchrony of pollen shed and silking between Bt and non-Bt corn hybrids. The dominant wind direction in both years was from WNW. However, in both years, there were also prevailing winds from SSW and WSW. Further studies are needed to quantify Bt levels in cross-pollinated kernels, measure the Bt dose of such kernels and associated lepidopteran pest survival, and measure the impact of Bt pollen on lepidopteran pests, particularly when considering the seed mixture refuge configuration.

Harmonia axyridis (Coleoptera: Coccinellidae) exhibits no preference between Bt and non-Bt maize fed Spodoptera frugiperda (Lepidoptera: Noctuidae).

A recent shift in managing insect resistance to genetically engineered (GE) maize consists of mixing non-GE seed with GE seed known as "refuge in a bag", which increases the likelihood of predators encountering both prey fed Bt and prey fed non-Bt maize. We therefore conducted laboratory choice-test feeding studies to determine if a predator, Harmonia axyridis, shows any preference between prey fed Bt and non-Bt maize leaves. The prey species was Spodoptera frugiperda, which were fed Bt maize (MON-810), expressing the single Cry1Ab protein, or non-Bt maize. The predators were third instar larvae and female adults of H. axyridis. Individual predators were offered Bt and non-Bt fed prey larvae that had fed for 24, 48 or 72 h. Ten and 15 larvae of each prey type were offered to third instar and adult predators, respectively. Observations of arenas were conducted at 1, 2, 3, 6, 15 and 24 h after the start of the experiment to determine the number and type of prey eaten by each individual predator. Prey larvae that fed on non-Bt leaves were significantly larger than larvae fed Bt leaves. Both predator stages had eaten nearly all the prey by the end of the experiment. However, in all combinations of predator stage and prey age, the number of each prey type consumed did not differ significantly. ELISA measurements confirmed the presence of Cry1Ab in leaf tissue (23-33 µg/g dry weight) and S. frugiperda (2.1-2.2 µg/g), while mean concentrations in H. axyridis were very low (0.01-0.2 µg/g). These results confirm the predatory status of H. axyridis on S. frugiperda and that both H. axyridis adults and larvae show no preference between prey types. The lack of preference between Bt-fed and non-Bt-fed prey should act in favor of insect resistance management strategies using mixtures of GE and non-GE maize seed.

Putting the brakes on a cycle: bottom-up effects damp cycle amplitude.

Pest population density oscillations have a profound effect on agroecosystem functioning, particularly when pests cycle with epidemic persistence. Here, we ask whether landscape-level manipulations can be used to restrict the cycle amplitude of the European corn borer moth [Ostrinia nubilalis (Hübner)], an economically important maize pest. We analysed time series from Minnesota (1963-2009) and Wisconsin (1964-2009) to quantify the extent of regime change in the US Corn Belt where rates of transgenic Bt maize adoption varied. The introduction of Bt maize explained cycle damping when the adoption of the crop was high (Minnesota); oscillations were damped but continued to persist when Bt maize was used less intensely (Wisconsin). We conclude that host plant quality is key to understanding both epidemic persistence and the success of intervention strategies. In particular, the dichotomy in maize management between states is thought to limit the spatial autocorrelation of O. nubilalis.

Novel Vip3A Bacillus thuringiensis (Bt) maize approaches high-dose efficacy against Helicoverpa zea (Lepidoptera: Noctuidae) under field conditions: Implications for resistance management.

Sweet corn, Zea mays L., transformed to express a novel vegetative insecticidal protein, Vip3A (event MIR162, Syngenta Seeds, Inc..), produced by the bacterium, Bacillus thuringiensis (Bt), was evaluated over four field seasons in Maryland and two field seasons in Minnesota for efficacy against the corn earworm, Helicoverpa zea (Boddie). Hybrids expressing the Vip3A protein and pyramided in hybrids also expressing the Cry1Ab Bt protein (event Bt11, ATTRIBUTE(®), Syngenta Seeds, Inc.) were compared to hybrids expressing only Cry1Ab or to genetically similar non-Bt hybrids each year. In addition to H. zea efficacy, results for Ostrinia nubilalis (Hübner) and Spodoptera frugiperda (J.E. Smith) are presented. Over all years and locations, the non-Bt hybrids, without insecticide protection, averaged between 43 and 100% ears infested with a range of 0.24 to 1.74 H. zea larvae per ear. By comparison, in the pyramided Vip3A x Cry1Ab hybrids, no larvae were found and only minimal kernel damage (likely due to other insect pests) was recorded. Hybrids expressing only Cry1Ab incurred a moderate level of H. zea feeding damage, with surviving larvae mostly limited to the first or second instar as a result of previously documented growth inhibition from Cry1Ab. These results suggest that the Vip3A protein, pyramided with Cry1Ab, appears to provide the first "high-dose" under field conditions and will be valuable for ongoing resistance management.

Implementing reduced-risk integrated pest management in fresh-market cabbage: influence of sampling parameters, and validation of binomial sequential sampling plans for the cabbage looper (Lepidoptera Noctuidae).

Populations of cabbage looper, Trichoplusiani (Lepidoptera: Noctuidae), were sampled in experimental plots and commercial fields of cabbage (Brasicca spp.) in Minnesota during 1998-1999 as part of a larger effort to implement an integrated pest management program. Using a resampling approach and the Wald's sequential probability ratio test, sampling plans with different sampling parameters were evaluated using independent presence/absence and enumerative data. Evaluations and comparisons of the different sampling plans were made based on the operating characteristic and average sample number functions generated for each plan and through the use of a decision probability matrix. Values for upper and lower decision boundaries, sequential error rates (alpha, beta), and tally threshold were modified to determine parameter influence on the operating characteristic and average sample number functions. The following parameters resulted in the most desirable operating characteristic and average sample number functions; action threshold of 0.1 proportion of plants infested, tally threshold of 1, alpha = beta = 0.1, upper boundary of 0.15, lower boundary of 0.05, and resampling with replacement. We found that sampling parameters can be modified and evaluated using resampling software to achieve desirable operating characteristic and average sample number functions. Moreover, management of T. ni by using binomial sequential sampling should provide a good balance between cost and reliability by minimizing sample size and maintaining a high level of correct decisions (>95%) to treat or not treat.

Optimizing Helicoverpa zea (Lepidoptera: Noctuidae) insecticidal efficacy in Minnesota sweet corn: a logistic regression to assess timing parameters.

Late-season plantings of sweet corn in Minnesota result in an abundant supply of silking corn, Zea mays L., throughout August to early September that is highly attractive to the corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae). During a 10-yr period, 1997-2006, insecticide efficacy trials were conducted in late-planted sweet corn in Minnesota for management of H. zea. These data were used to develop a logistic regression model to identify the variables and interactions that most influenced efficacy (proportion control) of late-instar H. zea. The pyrethroid lambdacyhalothrin (0.028 kg [AI]/ha) is a commonly used insecticide in sweet corn and was therefore chosen for use in parameter evaluation. Three variables were found to be significant (alpha = 0.05), the percentage of plants silking at the time of the first insecticide application, the interval between the first and second insecticide applications, and the interval between the last insecticide application and harvest. Odds ratio estimates indicated that as the percentage of plants silking at the time of first application increased, control of H. zea increased. As the interval between the first and second insecticide application increased, control of H. zea decreased. Finally, as the interval between the last insecticide application and harvest increased, control of H. zea increased. An additional timing trial was conducted in 2007 by using lambda-cyhalothrin, to evaluate the impact of the percentage of plants silking at the first application. The results indicated no significant differences in efficacy against late-instar H. zea at 0, 50, 90, and 100% of plants silking at the first application (regimes of five or more sprays). The implications of these effects are discussed within the context of current integrated pest management programs for late-planted sweet corn in the upper midwestern United States.