Effect of equine chorionic gonadotropin administration on day 8 post-partum on ovarian follicular development, uterine health and uterine involution in lactating dairy cows.

The objective was to evaluate the effect of equine chorionic gonadotropin (eCG) injection on day 8 postpartum on ovarian cyclicity, uterine health and uterine involution in lactating dairy cows. Lactating dairy cows [n = 34, (21 primiparous and 13 multiparous)] were enrolled in the study. Animals were stratified by calving date, parity and BCS, and randomly assigned to either control (CON n = 18) or eCG treatments (n = 16). On day 8 ±â€¯0.9 post-partum (pp), eCG treatment cows received a 2 ml i.m. injection (500 IU) of eCG and control treatment cows received a 2 ml i.m. injection of 0.9% sodium chloride. Ovaries were examined by transrectal ultrasound every second day from day 10 pp until ovulation or regression of the first follicle wave, and the diameter of the dominant follicle was recorded at each exam. Ultrasound exams were conducted on days 21, 28, 35 and 42 pp to measure the diameter of the cervix and the uterine horns. Vaginal discharge score (VDS) was recorded on a 1 to 5 scale on days 14, 21, 28, 35 and 42 pp. Endometrial cytology samples were collected on day 42 pp, and the percentage of polymorphonuclear neutrophils (PMN) determined. Milk samples were collected three times per week from day 14 pp until day 60 pp for P4 determination. Ovarian follicular development variables, ovarian function variables analysed and incidence of subclinical endometritis were not affected by treatment. There was a treatment by parity interaction for pp interval to commencement of luteal activity (P = 0.04) and number of luteal phases (P = 0.05), whereby eCG treatment caused an earlier pp commencement of luteal activity and a greater number of luteal phases in multiparous cows, but not in primiparous cows. A significant effect of treatment on uterine involution was observed (previously gravid horn: P = 0.03; previously non-gravid horn: P = 0.01) and uterine health status (P = 0.03), whereby eCG cows had smaller mean horn uterine diameter and lesser VDS than CON cows. In summary, parenteral administration of 500 IU eCG on day 8 post-partum did not affect resumption of ovarian cyclicity in all cows but did enhance uterine involution and VDS. In multiparous cows, eCG advanced commencement of luteal activity and increased the number of luteal phases.

The relationship between anogenital distance and fertility, and genome-wide associations for anogenital distance in Irish Holstein-Friesian cows.

The evaluation of anogenital distance (AGD), the distance from the center of the anus to base of the clitoris, as a potential fertility trait for genetic selection in dairy cows has generated recent interest. The objectives of this cross-sectional observational study were to (1) characterize the distribution and variability of AGD, (2) determine factors associated with AGD, (3) estimate heritability for AGD, (4) identify single nucleotide polymorphisms (SNP) associated with phenotypic variation of AGD, and (5) validate the relationship between categories of AGD and fertility in Irish Holstein-Friesian cows. Anogenital distance was measured using digital calipers in 1,180 Holstein cows (mean ± standard deviation: 225 ± 79 d in milk) from 10 dairy herds located in Munster, Ireland. In addition, age (yr), weight (kg), height at hip (cm), and body condition score (BCS) at the time of AGD measurement were determined in a subset of 281 cows. Genotype information available from 908 cows was subsequently imputed to the Illumina Bovine High Density BeadChip (Illumina Inc., San Diego, CA) for genome-wide association analysis of phenotypic variation in AGD. Overall, AGD had a normal distribution and high variability (mean ± standard deviation; 119.2 ± 11.6 mm). Anogenital distance was weakly but positively associated with cow age, hip height, and body weight, and negatively associated with BCS; the phenotypic variation in AGD that was explainable by these variables was small (coefficient of determination; R2 = 0.09, 0.06, 0.10, and 0.02, respectively). The estimated heritability for AGD was 0.37 (standard error of mean ± 0.08). Six SNP of suggestive significance were identified on Bos taurus autosomes 6, 15, 20, and 26; however, none of these SNP was related to previously identified candidate genes for fertility. Cows were categorized into quartiles (Q1; 86 to 111 mm; n = 311, Q2; 112 to 120 mm; n = 330; Q3; 121 to 127 mm; n = 265, and Q4; 128 to 160 mm; n = 274) based on AGD and the association with reproductive outcomes examined (21-d submission rate, pregnancy to first AI, pregnancy rate within 21, 42 and 84-d after the farm mating start date, and number of times bred). None of the reproductive variables differed significantly between AGD categories. In summary, despite identification of high variability and moderate heritability for AGD in Irish Holstein-Friesian cows, reproductive outcomes did not differ between categories of AGD. This latter result differs from our previous finding of an inverse relationship between AGD and pregnancy outcomes in first- and second-parity Canadian Holstein cows, emphasizing the need to test and validate this new phenotype in diverse cow populations.