Attenuation of cell migration and induction of cell death by aged garlic extract in rat sarcoma cells.

Previous studies have shown that aged garlic extract suppresses cancer growth and enhances immune system against cancer, and yet little is known about inhibition of the cancer cell migration. In this study we investigated whether the aged garlic extract inhibits growth and migration of rat sarcoma tumor cells. The suppression of tumor cell growth was demonstrated by counting the cell number in three groups (control, cultured with 10 mg/ml, 20 mg/ml of aged garlic extracts) after culturing for 3 days and 5 days. The results showed that aged garlic extract inhibited the growth of rat sarcoma cancer cells in a dose-dependent manner, compared to the numbers of the cells grown in control group. The inhibition of tumor cell migration was examined by measuring the distance of trails left behind by the tumor cells when they passed through the polybeads overnight in four groups (control, 5 mg/ml, 10 mg/ml, 20 mg/ml aged garlic extracts). The average distance of trails in control group was 7.44 mm, whereas the average distance of cell movement is only 2.48 mm when treated with the highest concentration (20 mg/ml) of the aged garlic extract. The results also showed that the inhibitory effect of aged garlic extracts on tumor cell migration was dose-dependent. This is the first report to show that the aged garlic extract inhibits rat sarcoma cell migration, a critical feature of tumor cell metastasis. It can be thus envisioned that if tumor cell metastasis could be attenuated if not completely stopped, it would be possible to stabilize the tumor in the local area for surgical removal. The results suggest that garlic, as a natural plant, unlike other cancer treatment methods, may play a role in fighting cancer without significant side effects.

CHARGE association in a child with de novo inverted duplication (14)(q22-->q24.3).

We report on a 4-1/2 year old girl with apparent CHARGE association who had a de novo inverted duplication (14)(q22-->24.3), iris colobomas, ventricular septal defect, soft tissue choanal atresia, intellectual impairment, growth retardation, sensorineural deafness, apparently low set ears, and upslanting palpebral fissures. Family history was unremarkable and parental chromosomes were normal. Similarities between this and previously reported cases of 14q duplication suggest that a locus for a gene or genes causing some of the anomalies of CHARGE association may reside in the region 14q22 to 24.3.

Lymphokine expression profile of resting and stimulated CD4+ CTL clones specific for the glycoprotein of vesicular stomatitis virus.

A panel of long-term murine T lymphocyte clones specific for the glycoprotein of vesicular stomatitis virus (VSV) in association with either H-2I-Ad or I-E(d) was tested for the production of cytokines in both resting and poststimulation states using both in situ hybridization and bioassay. All but one of the clones showed antigen-specific cytolytic activity in a 4-hr 51Cr release assay. Unexpectedly, the clones did not appear to be typical Th1 cells. Five of these T cell clones produced both IL-2 and IFN-gamma but not IL-4 after stimulation with either phorbol 12-myristate 13-acetate (PMA) or concanavalin A (Con A). Some clones constitutively expressed mRNA for IL-2 and INF-gamma. The proliferation of these clones was factor independent, suggesting an autocrine growth mechanism. Three clones produced variable levels of IL-4 mRNA and some, to significant quantities, of IL-2 mRNA. One cytolytic clone produced neither IL-2 nor IL-4 mRNA to detectable levels, although mRNA for IFN-gamma was observed. A noncytolytic, Ag-specific clone produced IL-6, tumor necrosis factor (TNF), and lymphotoxin (LT), but no IL-2, IL-4, or IFN-gamma mRNA. There was a strong quantitative correlation between the expression of IL-2-, INF-gamma-, and LT-specific mRNAs by the clones. All the T cell clones tested which secreted INF-gamma and LT expressed no measurable IL-4 mRNA. We examined expression of several other genes in the panel of clones. These included TNF, met-enkephalin (met-enk), IL-1, and IL-6. IL-1 m-RNA synthesis was not observed in any of the T cell clones. Almost all clones produced TNF mRNA. Parallel bioassays showed that secreted IL-2/IL-4 activity levels and mRNA levels correlated well for all clones. Thus, we observed a great degree of heterogeneity among CD4+ cytolytic T lymphocyte clones.

Differences in the membrane skeleton proteins of RBC of AMoL patients.

We report here the different components of erythrocyte membrane skeleton proteins between acute monocytic leukemic (AMoL) patients and normal people. The bands in the 2 region of ghost membrane from AMoL patients exhibited significant differences on SDS gel electrophoresis. Band 2.2 was found to be missing and a "new" band with molecular weight (MW) 161,000 appeared. Also band 4.9 was missing, and the amounts of spectrin, actin, and band 4.8 of AMoL patients were decreased markedly. No such alterations can be seen in normal individuals, even in acute myeloid leukemic (AML) and chronic myeloid leukemic (CML) patients.