RESUMO
Wastewater from shrimp farming is rich in organic material, solids, and nutrients, which cause a series of environmental problems when released into the environment. Currently, for the removal of nitrogen compounds from wastewater, among the most studied methods is biological denitrification. The objective of this study was to evaluate the operational parameters for the development of a more sustainable technology for the removal of nitrogen compounds from shrimp farm wastewater, using Bambusa tuldoides (a species of bamboo) as a source of carbon and a material conducive to the development of selected denitrifying bacteria. To optimize the process, biological denitrification assays were performed varying the following parameters: bamboo length (cm), pH, temperature, and stoichiometric proportions of C and N. The operational stability of the process with the reuse of the bamboo biomass was also evaluated. Cronobacter sakazakii and Bacillus cereus were identified as denitrifying microorganisms present in reactor with bamboo biomass. The best operational conditions observed were pH 6 to 7 and temperature 30 to 35 °C, and the addition of an external carbon source was not necessary for the denitrification process to occur efficiently. Under these conditions, biological denitrification occurred with an average efficiency above 90% based on the removal of the nitrogen contaminants evaluated (NO3-N and NO2-N). Regarding operational stability, 8 cycles were performed using the same source of carbon without reducing the efficiency of the process.