Phenotypic Plasticity Index as a Strategy for Selecting Water-Stress-Adapted Coffee Genotypes.

The adaptive potential of plants is commonly used as an indicator of genotypes with higher breeding program potential. However, the complexity and interaction of plant metabolic parameters pose a challenge to selection strategies. In this context, this study aimed to explore phenotypic plasticity within the germplasm of Hybrid Timor coffee. Additionally, we assessed the utility of the multivariate phenotypic plasticity index (MVPi) as a promising tool to predict genotype performance across diverse climatic conditions. To achieve this, we evaluated the performance of seven accessions from the Hybrid Timor germplasm in comparison to the Rubi and IPR 100 cultivars, known for their susceptibility and resistance to drought, respectively. The experiment took place in a greenhouse under two conditions: one with normal soil moisture levels near maximum capacity, and the other with a water deficit scenario involving a period of no irrigation followed by rehydration. Data on physiological and biochemical factors were collected at three stages: before applying the water deficit, during its imposition, and after rehydration. Growth data were obtained by the difference between the beginning and end of the experimental period Furthermore, field evaluations of the productivity of the same genotypes were carried out over two consecutive seasons. Based on physiological and biochemical assessments, the MVPi was computed, employing Euclidean distance between principal component multivariate analysis scores. Subsequently, this index was correlated with growth and productivity data through linear regressions. Our findings reveal that the plastic genotypes that are capable of significantly altering physiological and biochemical parameters in response to environmental stimuli exhibited reduced biomass loss in both aerial and root parts. As a result, this positively influenced their productivity. Enhanced plasticity was particularly prominent in accessions from the MG Germplasm Collection: MG 311-Hybrid Timor UFV 428-02, MG 270-Hybrid Timor UFV 377-21, and MG 279-Hybrid Timor UFV 376-31, alongside the Rubi MG 1192 cultivar. The MVPi emerged as a valuable instrument to assess genotype adaptability and predict their performance under varying climatic scenarios.

IBA and melatonin increase trigonelline and caffeine during the induction and initiation of adventitious roots in Coffea arabica L. cuttings.

Caffeine and trigonelline are found in Coffea arabica, and show antioxidant roles and growth and development functions. However, there are no reports on trigonelline and caffeine in relation to coffee rooting. The aim was to evaluate the impact of application of indole-3-butyric acid (IBA) and melatonin on caffeine and trigonelline at different stages of adventitious rooting in cuttings. In addition, to study the correlation between these metabolites and H2O2, phenols, and antioxidant enzymes. Four treatments (Control, melatonin 21 µM (M21), melatonin 43 µM (M43), and IBA 7380 µM (IBA)) were used, with four replications. The growth and biochemical parameters of the antioxidant system were performed in induction, initiation, and extension rooting stages. Higher concentrations of trigonelline and caffeine quantified in the induction and initiation stages were positively correlated with higher percentage of rooted cuttings. Trigonelline and caffeine were positively correlated with H2O2 in all stages of development of adventitious roots. The correlations of trigoneline and caffeine with phenols and antioxidant enzymes reveal different profiles, depending on the phases. The results indicate that IBA and melatonin increase trigonelline and caffeine during the induction and initiation of adventitious roots in Coffea arabica cuttings, which is correlated with a higher percentage of rooted cuttings.

Purple acid phosphatases in coffee-genome-wide identification and the transcriptional profile of selected members in roots upon Pi starvation.

Phosphorus (P) availability is determinant for crop productivity and, despite the sufficient amount of this nutrient in arable land, most of it remains insoluble, leading to the need of high fertilizer input. To cope with P scarcity forecasts and also for cropping costs alleviation, genotypes better adapted to promote P solubilization and absorption are required, especially for perennial crops. Coffee is one of these important perennial crops cultivated in soils with low P availability, and thus we aimed to study adaptive strategies to coffee genotypes in acquire phosphorus. In this study, we focused on rhizosphere phosphatase activity, a major characteristic related to P solubilization from organic pools. To explore the genetic basis of this characteristic, we firstly identified 29 Purple Acid Phosphatases (PAP) genes in C. canephora genome and selected five candidates with higher potential to encode secreted PAPs. We found that C. arabica genotypes have diverse profiles of rhizosphere phosphatase activity, as well as microbial biomass carbon, which we measured to explore the impact of the plant on microbiota and also to discriminate the phosphatase activity origin (plant or microorganism-derived). We selected two C. arabica cultivars with contrasting phosphatase activity and found that one PAP gene has a correlated gene expression profile with this characteristic. This work explores coffee adaptative responses to P starvation conditions, and the information provided can further contribute to breeding programs aiming better adapted genotypes for sustainable agriculture in face of current challenges. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03399-6.

Influence of postharvest processing on the quality and sensory profile of groups of arabica coffee genotypesc.

BACKGROUND: This study combined qualitative and quantitative approaches to obtain a better understanding of the sensory quality of beverages made from Arabic coffee genotypes subjected to different processing methods. Over 3 consecutive years, 270 accessions of Arabic coffee from the germplasm collection of Minas Gerais State, Brazil, were sensorially characterized after dry postharvest processing. At the end of this period, the 26 genotypes with the greatest potential for the production of specialty coffees were subjected to dry and wet processing. Granulometry and sensory quality were evaluated by scoring and describing the sensory profiles of the samples. RESULTS: Adequate management during all postharvest stages maintained the potential coffee quality, regardless of processing. All of the coffees studied were classified as special. There was variation in the perceived nuances of the sensory attributes among the groups of genotypes and as a function of postharvest processing, with emphasis on the increased frequency of high levels of sweetness in wet processing. Among the aroma/flavor sensory attributes, the caramel subcategory, as a long and pleasant aftertaste, were predominant in all of the genotypes studied, regardless of the type of processing. CONCLUSION: The differences in the perceptions of aroma/flavor and aftertaste in different processes were easier to identify in the commercial cultivars studied, as well as in the Bourbon accessions of Timor Hybrid and their derivatives. The access MG 0159 Maragogipe Hybrid F1 stood out in terms of all of the evaluated characteristics, regardless of the processing method used. © 2022 Society of Chemical Industry.

Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance.

The aim of this study was to identify the correlation between photochemical efficiency and candidate genes expression to elucidate the drought tolerance mechanisms in coffee progenies (Icatu Vermelho IAC 3851-2 × Catimor UFV 1602-215) previously identified as tolerant in field conditions. Four progenies (2, 5, 12 and 15) were evaluated under water-deficit conditions (water deficit imposed 8 months after transplanting seedlings to the pots) and under irrigated system. Evaluations of physiological parameters and expression of candidate genes for drought tolerance were performed. Progeny 5 showed capacity to maintain water potential, which contributed to lower qP variation between irrigated and deficit conditions. However, the increases of qN and NPQ in response to stress indicate that this progeny is photochemically responsive to small variations of Ψam protecting the photosystem and maintaining qP. Data obtained for progeny 12 indicated a lower water status maintenance capacity, but with increased qN and NPQ providing maintenance of the ɸPSII and ETR parameters. A PCA analysis revealed that the genes coding regulatory proteins, ABA-synthesis, cellular protectors, isoforms of ascorbate peroxidase clearly displayed a major response to drought stress and discriminated the progenies 5 and 12 which showed a better photochemical response. The genes CaMYB1, CaERF017, CaEDR2, CaNCED, CaAPX1, CaAPX5, CaGolS3, CaDHN1 and CaPYL8a were up-regulated in the arabica coffee progenies with greater photochemical efficiency under deficit and therefore contributing to efficiency of the photosynthesis in drought tolerant progenies.

Chemical and sensory characteristics in the selection of bourbon genotypes

The evaluation of coffee quality in Brazil for commercialization is conducted mainly through sensory analysis, also known as the "cup test", in which professional tasters evaluate and score various attributes. The adoption of chemical methods could complement the sensory classification of beverages, if correlations between these chemical and sensory analyses exist, making classification less subjective. This work aimed to identify the relationships between the chemical and sensorial traits of coffee-beverage quality and to evaluate the use of these traits as criteria for the selection of Bourbon cultivars. Twenty coffee genotypes from the first three harvests across five municipalities of the state of Minas Gerais, Brazil were evaluated. The genotypic values, predicted for each genotype, were used to determine the index based on the sum of ranks from Mulamba and Mock. The genetic correlations among the evaluated traits were also estimated. The presented evaluations were not able to efficiently detect genetic and phenotypic relationships between the chemical and sensorial characteristics of drink quality, but as selection criteria for generation advancement in the beverage quality, it is possible to use these characteristics. Bourbon Amarelo LCJ 9-IAC, Bourbon Amarelo-Procafé, Bourbon Amarelo-Boa Vista, Bourbon Vermelho-São João Batista, and Bourbon Amarelo-Samambaia were the genotypes with the most promising cup quality in the studied regions. Through the selection of these five genotypes, the selection gain was 1.65% for sensory score for beverage quality, when the interaction among the studied environments was removed. The heritability was 92% for improving this trait.

Discrimination of genotypes coffee by chemical composition of the beans: Potential markers in natural coffees.

The chemical composition of coffee beans varies according to the cultivar used, but there is little information about the differences among them resulting in different sensory profiles. The purpose was to find potential chemical markers to discriminate genealogical groups of coffee using regression analysis by partial least squares. Twenty-two accessions of C. arabica were used with two repetitions each. There were chromatographic and physical-chemical analyses to determine the composition of raw beans and chemometric analysis as the PCA and the PLS-DA. The analysis of PCA did not showed detailed information about the differences between the groups. The model PLS-DA identified the most important variables in the discrimination of the genealogical groups. Arachidic acid and stearic acid the markers for the Bourbon group; the myristic and linoleic acids and sucrose for the Exotic group; and lauric, palmitoleic and oleic acids, and the protein content to the Timor Hybrid group.

Leaf water potential of coffee estimated by landsat-8 images.

Traditionally, water conditions of coffee areas are monitored by measuring the leaf water potential (ΨW) throughout a pressure pump. However, there is a demand for the development of technologies that can estimate large areas or regions. In this context, the objective of this study was to estimate the ΨW by surface reflectance values and vegetation indices obtained from the Landsat-8/OLI sensor in Minas Gerais-Brazil Several algorithms using OLI bands and vegetation indexes were evaluated and from the correlation analysis, a quadratic algorithm that uses the Normalized Difference Vegetation Index (NDVI) performed better, with a correlation coefficient (R2) of 0.82. Leave-One-Out Cross-Validation (LOOCV) was performed to validate the models and the best results were for NDVI quadratic algorithm, presenting a Mean Absolute Percentage Error (MAPE) of 27.09% and an R2 of 0.85. Subsequently, the NDVI quadratic algorithm was applied to Landsat-8 images, aiming to spatialize the ΨW estimated in a representative area of regional coffee planting between September 2014 to July 2015. From the proposed algorithm, it was possible to estimate ΨW from Landsat-8/OLI imagery, contributing to drought monitoring in the coffee area leading to cost reduction to the producers.

Agronomic traits of coffee tree progenies from Timor Hybrid x Catuaí crossing / Características agronômicas de progênies de cafeeiro oriundas do cruzamento de Híbrido de Timor x Catuaí

The objective of this work was to evaluate the agronomic traits of coffee tree progenies. The experiment was set in Ouro Verde Farm, located at Campos Altos County, MG. Twenty three coffee progenies and seven control cultivars were used. These progenies are from the fourth generation of crossing between Timor Hybrid and Catuaí. The experiment was set in a randomized block design with four replicates, a total of 120 plots with 8 plants per plot. The yield of processed coffee sacks ha-1 was evaluated in eight crop years from the 2003 to the 2011 harvests. The percentage of fruits at the cherry stage, floating fruits, coffee classification (sieve above 17), income and plant vigor were evaluated in the 2010 and in the 2011 harvests. It was concluded that the progenies showed a great variability for the agronomic traits. Progenies 514-7-4-C130, 493-1-2-C134 and 518-2-10-C408 had the highest yield in the four two-year periods. The progenies 436-1-4-C26, 516-8-2-C109, 493-1-2-C134, 518-2-10-C408, 514-7-16-C211 and 514-7-16-C208 presented the highest values for plant vigor. Progeny 493-1-2-C134 stood out in all analyzed traits, showing to be promising for the advance of the generations.

Objetivou-se com este trabalho avaliar o comportamento agronômico de progênies de cafeeiro. O experimento foi instalado na Fazenda Ouro Verde, situada no Município de Campos Altos-MG, compreendendo vinte e três progênies e sete cultivares utilizadas como testemunhas. Essas progênies referem-se à quarta geração do cruzamento entre Híbrido de Timor e Catuaí. Foi utilizado o delineamento de blocos ao acaso, com quatro repetições, totalizando 120 parcelas, sendo cada parcela constituída por oito plantas. A produtividade em sacas de café beneficiado ha-1 foi avaliada em oito colheitas, de 2003 a 2011. Nas colheitas de 2010 e 2011 foram avaliadas a porcentagem de frutos no estádio cereja e de frutos chochos, a classificação do café (peneira 17 acima), renda e vigor vegetativo. Conclui-se que as progênies apresentaram grande variabilidade para as características agronômicas estudadas. As progênies 514-7-4-C130, 493-1-2-C134 e 518-2-10-C408 apresentaram os maiores valores de produtividade na média dos quatro biênios avaliados. As progênies 436-1-4-C26, 516-8-2-C109, 493-1-2-C134, 518-2-10-C408, 514-7-16-C211 e 514-7-16-C208 apresentaram as maiores notas de vigor vegetativo. A progênie 493-1-2-C134 se destacou em todas as características analisadas, mostrando-se promissora para o avanço de gerações.

Comportamento de cultivares de cafeeiros C. Arabica L. enxertados sobre cultivar 'Apoatã IAC 2258' (Coffea canephora) / Performance of C. Arabica L. Coffee cultivars grafted on 'Apoatã IAC 2258' cultivar (Coffea canephora)

Objetivou-se no trabalho avaliar a influência da enxertia e do porta-enxerto 'Apoatã IAC 2258' no comportamento agronômico de sete cultivares de cafeeiro da espécie Coffea arabica cultivadas em solo isento de fitonematoides. O ensaio foi conduzido em delineamento experimental de blocos casualizados em esquema fatorial (7 x 3) com quatro repetições, sendo sete cultivares de Coffea arabica ('Obatã IAC 1669-20', 'Acauã', 'Oeiras MG 6851', 'Catucaí Amarelo 2SL', 'Topázio MG 1190', 'IBC Palma II' e 'Paraíso MG H 419-1') e três tipos de enxertia (muda enxertada, auto enxertada e pé franco). Foi avaliada a produtividade média de quatro anos, uniformidade de maturação, percentagem de frutos chochos e classificação do grão por tipo de peneira. As cultivares enxertadas estudadas apresentaram comportamento agronômico satisfatório em todas as características avaliadas e semelhante ao pé-franco. Em áreas isentas de fitonematoides, à exceção das cultivares 'IBC Palma II' e 'Paraíso MG H 419-1', as demais cultivares estudadas são adequadas opções de escolha para uso como copas. A técnica da enxertia com o uso do porta-enxerto 'Apoatã IAC 2258' em área isenta de fitonematoides não se justifica para incremento de produtividade.

This research had the objective to evaluate the effect of grafting and 'Apoatã IAC 2258' cv. as rootstock on agronomic performance of seven Coffea arabica cvs. planted in area nematodes free. The experimental design was randomized blocks using a 7x3 factorial scheme with four repetitions. The treatments were 'Obatã IAC 1669-20', 'Acauã', 'Oeiras MG 6851', 'Catucaí Amarelo 2SL', 'Topázio MG 1190', 'IBC Palma II' and 'Paraíso MG H 419-1' coffee cultivars grafted on 'Apoatã IAC 2258', self grafted (meaning a cultivar was grafted on a rootstock of the same cultivar) and no grafting. The characteristic evaluated was the average yield of beans between the first and the fourth year, the fruit maturation stage, floating grain percentage and the bean size. The grafting cultivars showed good agronomic performance in the characteristic evaluated. The performance in coffee cultivars grafted on 'Apoatã IAC 2258' is the same in no grafting. In nematodes free areas, with the exception of the cultivars 'IBC Palma II' and 'Paraíso MG H 419-1', all cultivars are good choices to be used as scions. The grafting technique using 'Apoatã IAC 2258' as rootstock on area free of nematodes is not justified for yield increase.

Efeito de recipientes e substratos utilizados na produção de mudas de cafeeiro no desenvolvimento inicial em casa de vegetação, sob estresse hídrico / Effect of different recipients and substrata used in the production of coffee tree seedlings in the initial development in greenhouse under water stress

Com o objetivo de avaliar os efeitos de recipientes e substratos no desenvolvimento inicial de cafeeiros (Coffea arabica L.) plantados em vasos dentro de casa de vegetação, sob diferentes níveis de estresse hídrico, foi conduzido um experimento no Setor de Cafeicultura da Universidade Federal de Lavras - UFLA, no período de janeiro a maio de 2004. O delineamento experimental utilizado foi em blocos casualizados (DBC) em esquema fatorial (3 x 3 x 4), sendo o primeiro fator constituído de três recipientes utilizados na produção das mudas, tubetes de polietileno rígido de 50 mL; tubetes de 120 mL; e saquinhos de polietileno, nas dimensões de 20 cm de altura por 10 cm de largura e capacidade volumétrica, aproximada, de 700 mL; o segundo fator constituído de três substratos utilizados na produção das mudas, substrato alternativo, composto por 65 por cento de casca de arroz carbonizada + 35 por cento de substrato comercial; substrato comercial Plantmax hortaliças HT; e substrato padrão, composto por 70 por cento de terra + 30 por cento de esterco bovino peneirados e o terceiro fator foi constituído por quatro intervalos entre irrigações, 2, 6, 10 e 14 dias. Observa-se que os recipientes de capacidades volumétricas diferentes e os substratos utilizados influenciam, significativamente, o desenvolvimento dos cafeeiros, sendo que, após 120 dias do transplante das mudas para vasos em casa de vegetação com diferentes níveis de estresse hídrico, as plantas provenientes de mudas produzidas em saquinhos de polietileno e tubetes de 120 mL contendo substratos alternativo e comercial apresentaram melhor desenvolvimento.

With the aim of evaluating the effects of different recipients and substrata in the production of coffee tree seedlings (Coffea arabica L.), planted in pots inside the greenhouse, under different water stress levels, an experiment was carried out in the Section of Coffee of Department of Agriculture of Universidade Federal de Lavras - UFLA, in the period from September of 2003 to January of 2004. The experimental outline used was randomized blocks (DBC) in a 3 x 3 factorial arrangement. The recipients used were rigid polyethylene tube with a capacity of 50 mL; 120 mL tubes; and 20cm-high, 10cm-wide polyethylene sacks, with an approximate capacity of 700 mL. The second factor consisted of three substrata used for seedling production: an alternative substratum, composed of 65 percent of charred rice peel + 35 percent of commercial substratum; the commercial substratum, Plantmax vegetables HT; and the standard substratum, composed of 70 percent of earth + 30 percent of bovine drizzled manure. The third factor consisted of four intervals (2, 6, 10 and 14 days) between irrigations The results obtained allow concluding that the recipients and the substrata used significantly influenced the development of the coffee trees. 120 days after the transplantation of the seedlings to pots in the greenhouse with different levels of water stress, we could conclude that larger recipients (polyethylene sack and 120 mL tube) filled with alternative and commercial substrata provided better seedling development.

Estudo anatômico de cafeeiros enxertados / Anatomic study of grafted coffee trees

Neste trabalho, objetivou-se, avaliar as características anatômicas do caule de cafeeiros enxertados, auto-enxertados e pé-franco. Foram utilizadas as cultivares Catucaí Amarelo 2 SL e Topázio MG 1190 (Coffea arabica L.) e o porta-enxerto Apoatã IAC 2258 (C. canephora Pierre ex Froehn.). As seções anatômicas longitudinais e transversais foram realizadas aos 30, 60 e 90 dias após a enxertia. Os resultados não mostraram evidências de incompatibilidade de reconstituição e formação de tecidos entre as cultivares estudadas.

This work aimed at evaluating the anatomic features of grafted, self-grafted and ungrafted coffee tree stems. The longitudinal and transversal sections were performed at 30, 60, and 90 days after grafting in Catucaí Amarelo 2 SL and Topázio MG 1190 (Coffea arabica L.) cultivars, as well as in the Apoatã rootstock. The results did not show evident signs of incompatibility of tissue reconstruction and formation between the cultivars studied.

Produção de cafeeiros Coffea arabica L. pés francos autoenxertados e enxertados em apoatã IAC 2258 / Production of coffee plants grafted on Apoatã IAC 2258

Visando obter informações sobre a produção de sete cultivares de C. arábica L. pé franco, auto-enxertados e enxertados no porta enxerto C. canephora Pierre ex Froehn cultivar Apoatã IAC 2258, foi instalado e conduzido este ensaio, em condições de campo isento de nematóides, de janeiro de 2004 a junho de 2006. O delineamento experimental utilizado foi em blocos casualizados, num esquema fatorial (7 x 3) com quatro repetições, sendo sete cultivares de Coffea arabica L. e três tipos de mudas (enxertada, autoenxertada e pé franco). Os resultados da primeira produção mostraram que as plantas enxertadas produziram menos que as outras auto-enxertadas e pé franco, independentemente da cultivar. Não houve diferenças significativas entre cafeeiros pé franco e autoenxertados. Não se recomenda o cultivo de cafeeiros Coffea arábica L. enxertados em Cofea canephora Pierre ex Froehn, cultivar Apoatã IAC 22587, em área isenta de nematóides. A cultivar Paraíso produziu menos que as demais.

Aiming to get information about the production of seven cultivars of coffee trees (Coffea arabica L.), ungrafted, self grafted or grafted on Apoatã IAC 2258 (Coffea canephora Pierre ex Froehn) rootstock, this essay was installed and carried out on a nematode-free-land from January 2004 to June 2006. The experimental outline used was the randomized blocks with a 7 x 3 factorial split-plot arrangement and four replications, as follows: seven Coffea arabica L. cultivars and three types of seedling (grafted, selfgrafted and ungrafted). The results obtained from the first production showed that grafted seedlings yielded less than the self grafted and ungrafted plants, regardless of the cultivar. No significant differences were found between the ungrafted and the self-grafted coffee trees. Coffee trees (Coffea arabica L.) grafted on Coffea canephora Pierre ex Froehn, cultivar Apoatã IAC 2258 are not recommended for nematoid-free land. The Paraiso cultivar was less productive than the other cultivars.

Comportamento de progênies F4 obtidas por cruzamentos de 'Icatu' com 'Catimor' / Behavior of F4 progenies obtained from crossing of Icatu with Catimor

Conduziu-se este trabalho com o objetivo de avaliar progênies de cafeeiro resultantes do cruzamento entre 'Icatu' x 'Catimor', quanto à produtividade e vigor vegetativo e selecionar progênies superiores para avançar no programa de melhoramento dessa população. O delineamento utilizado foi em blocos casualizados com quatro repetições, 30 tratamentos (29 progênies do cruzamento mais a cultivar Rubi MG 1192, utilizada como testemunha). Para análise de variância dos dados adotou-se o esquema de parcela subdividida no tempo. Foram analisados a produção em sacas beneficiadas.ha-1 de seis colheitas 2001/2002 a 2006/2007 e o vigor vegetativo avaliado após a colheita 2006/2007. Os resultados obtidos permitem verificar a presença da variabilidade genética para produção entre as progênies avaliadas, fato confirmado pela alta estimativa da maioria das progênies estudadas, demonstraram que é possível selecionar progênies superiores na população estudada.

The objective of this research was to evaluate coffee progenies resulted from the crossing of 'Icatu' with 'Catimor' in relation to their production and plant vigor and to select higher quality progenies to advance the breeding program of this population. For this purpose, a randomized complete block design was carried out with four replications and for the analysis of variance, the Split plot temporal arrangement was adopted. Twenty nine progenies plus the Rubi MG 1192 cultivar, used as a control, were evaluated. The yield of beans in bags.ha-1 of six harvests from 2001/2002 to 2006/2007 was evaluated and the plant vigor after the harvest was assessed. The results indicated that the evaluated progenies showed variability mainly for grain yield confirmed by heritability. This condition associated to high bean yields, which reached 62.0 bags.ha-1 and high plant vigor, showed the possibility of selecting outstanding progenies in the studied population.