Application of Rapeseed Meal Protein Isolate as a Supplement to Texture-Modified Food for the Elderly.

Rapeseed meal (RSM), a by-product of rapeseed oil extraction, is currently used for low-value purposes. With a biorefinery approach, rapeseed proteins may be extracted and recovered for high-end uses to fully exploit their nutritional and functional properties. This study reports the application of RSM protein isolate, the main output of a biorefining process aimed at recovering high-value molecules from rapeseed meal, as a supplement to texture-modified (TM) food designed for elderly people with mastication and dysphagia problems. The compositional (macronutrients by Official Methods of Analyses, and mineral and trace element profiles using Inductively Coupled Plasma Optical Emission Spectrometry ICP-OES), nutritional and sensory evaluations of TM chicken breast, carrots and bread formulated without and with RSM protein supplementation (5% w/w) are hereby reported. The results show that the texture modification of food combined with rapeseed protein isolate supplementation has a positive impact on the nutritional and sensory profile of food, meeting the special requirements of seniors. TM chicken breast and bread supplemented with RSM protein isolate showed unaltered or even improved sensory properties and a higher nutrient density, with particular regard to proteins (+20-40%) and minerals (+10-16%). Supplemented TM carrots, in spite of the high nutrient density, showed a limited acceptability, due to poor sensory properties that could be overcome with an adjustment to the formulation. This study highlights the potentialities of RSM as a sustainable novel protein source in the food sector. The application of RSM protein proposed here is in line with the major current challenges of food systems such as the responsible management of natural resources, the valorization of agri-food by-products, and healthy nutrition with focus on elderly people.

Valorization Potentials of Rapeseed Meal in a Biorefinery Perspective: Focus on Nutritional and Bioactive Components.

Rapeseed meal (RSM), a by-product of oilseed extraction connected to the agri-food and biofuel sectors, is currently used as animal feed and for other low-value purposes. With a biorefinery approach, RSM could be valorized as a source of bio-based molecules for high-value applications. This study provides a chemical characterization of RSM in the perspective of its valorization. A qualitative study of main functional groups by fourier transform infrared (FTIR) spectroscopy was integrated with a chemical characterization of macronutrients, minerals by inductively coupled plasma optical emission spectrometry (ICP-OES), phenolic acids and lipid components by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), HPLC-diode-array detector (HPLC-DAD) and gas chromatography-mass spectrometry/flame ionization detector (GC-MS/FID). The study, conducted on different lots of RSM collected over a one-year period from an oil pressing factory serving a biofuel biorefinery, highlighted a constant quality over time of RSM, characterized by high protein (31-34%), fiber (33-40%) and mineral (5.5-6.8%) contents. Polyphenol extracts showed a significant antioxidant activity and a prevalence of sinapic acid, accounting for more than 85% of total phenolic acids (395-437 mg kg-1 RSM). Results highlight the potentialities of RSM for further valorization strategies that may lead to the creation of new cross-sector interconnections and bio-based value chains with improvement of the economics and sustainability of the bioeconomy sectors involved.

Corn Bioethanol Side Streams: A Potential Sustainable Source of Fat-Soluble Bioactive Molecules for High-Value Applications.

This paper reports data from a characterization study conducted on the unsaponifiable lipid fraction of dry-grind corn bioethanol side streams. Phytosterols, squalene, tocopherols, tocotrienols, and carotenoids were quantified by High Performance Liquid Chromatography with Diode-Array Detector (HPLC-DAD) and Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) in different lots of post-fermentation corn oil and thin stillage collected from a bioethanol plant over a time-span of one year. Fat-soluble bioactives were present at high levels in corn oil, with a prevalence of plant sterols over tocols and squalene. Beta-sitosterol and sitostanol accounted altogether for more than 60% of total sterols. The carotenoid profile was that typical of corn, with lutein and zeaxanthin as the prevalent molecules. The unsaponifiable lipid fraction profile of thin stillage was qualitatively similar to that of post-fermentation corn oil but, in quantitative terms, the amounts of valuable biomolecules were much lower because of the very high dilution of this side stream. Results indicate that post-fermentation corn oil is a promising and sustainable source of health-promoting bioactive molecules. The concomitant presence of a variegate complex of bioactive molecules with high antioxidant potentialities and their potential multifaceted market applications as functional ingredients for food, nutraceutical, and cosmeceutical formulations, make the perspective of their recovery a promising strategy to create new bio-based value chains and maximize the sustainability of corn dry-grind bioethanol biorefineries.

Towards a Valorization of Corn Bioethanol Side Streams: Chemical Characterization of Post Fermentation Corn Oil and Thin Stillage.

First-generation biofuel biorefineries may be a starting point for the development of new value chains, as their by-products and side streams retain nutrients and valuable molecules that may be recovered and valorized for high-value applications. This study provides a chemical characterization of post-fermentation corn oil and thin stillage, side streams of dry-grind corn bioethanol production, in view of their valorization. An overall long-term study was conducted on the two co-products collected over 1 year from a bioethanol plant. Water content, acid value, sedimentation, mineral composition, and fatty acid profiles were analyzed on post-fermentation corn oil. Results highlighted that its acid value was high (19.72-24.29 mg KOH/g), indicating high levels of free fatty acids, but stable over the year due to standardized operating conditions. The fatty acid profile was that typical of corn oil, with a prevalence of linoleic (54-59% of total fatty acids) over oleic (23-27%) and palmitic (12-17%) acids. Macronutrients, fatty acid, and mineral profiles were investigated in thin stillage. Results revealed the acidic pH (4.05-4.68) and high dilution (90-93% water) of this side stream. The dry mass was composed of fats (19-30%), proteins (8.8-12.8%), ash (8.7-9.5%), and fiber (7.3-9.8%). The concomitant presence of a variegate complex of molecules of nutritional interest in corn bioethanol co-products, with several potential high-value market applications, make the perspective of their recovery a promising strategy to create new cross-sector interconnections according to circular economy principles.

Carotenoid profiling of five microalgae species from large-scale production.

The carotenoid profiles of biomass from five eukaryotic microalgae, Porphyridium cruentum, Isochrysis galbana, Phaeodactylum tricornutum, Tetraselmis suecica and Nannochloropsis gaditana, produced at an industrial plant in outdoor photobioreactors, were investigated. Pigments were solvent-extracted after an ultrasonic pre-treatment and separated by HPLC-photodiode-array using a reversed-phase C18 column. Microalgae showed species-specific carotenoid profiles. Carotenoids were mostly in their free form, with a prevalence of xanthophylls over carotenes. Beta-carotene was the only carotenoid common to all species. The Rhodophyta P. cruentum exhibited the lowest total carotenoid content (167.2 mg 100 g-1 dw) and the simplest profile, with (all-E)-zeaxanthin (94.2 mg 100 g-1 dw, 56% of total carotenoids) and (all-E)-ß- carotene (53.4 mg 100 g-1 dw, 32% of total carotenoids) as the major carotenoids. The Haptophyta Isochrysis galbana and the Bacillariophyta Phaeodactylum tricornutum were the species with the highest total carotenoid content (1760 mg and 1022 mg 100 g-1 dw, respectively). These species were characterized by similar carotenoid profiles, with (all-E)-fucoxanthin as the chief compound (1346 mg and 776.8 mg 100 g-1 dw for I. galbana and P. tricornutum, respectively), accounting for about 76% of total carotenoids. The Chlorophyta Tetraselmis suecica was the species showing the greatest variety of carotenoids, with both α- carotene and ß- carotene and their derivatives present. (All-E)-lutein (85.4 mg 100 g-1 dw) and (all-E)-violaxanthin (81.8 mg 100 g-1 dw) were the major pigments in this species. In the Ochrophyta Nannochloropsis gaditana, (all-E)-violaxanthin was the prevalent carotenoid (336.7 mg 100 g-1 dw), followed by (all-E)-ß-carotene (100.1 mg 100 g-1 algal dw). The carotenoid content of the microalgal biomass studied compared favourably to that of major vegetable sources. Due to their characteristics, these microalgae, most of them currently finding their main application in aquaculture, may be also regarded as valuable sources of carotenoids to be used in the formulation of functional food and nutraceuticals.

Total mercury levels in crustacean species from Italian fishery.

This study investigated mercury contamination levels in eight commercially valuable crustacean species caught off the Central Adriatic and Tyrrhenian coasts of Italy. Total mercury levels were measured by Thermal Decomposition-Amalgamation-Atomic Absorption Spectrophotometry. Results showed a high variability among species with values ranging from 0.070 to 1.24 (mg kg-1 wet weight). The lowest mercury levels were detected in caramote prawn (Penaeus kerathurus), warty crab (Eriphia verrucosa) and European spider crab (Maja squinado), decapods living in shallow waters. Levels exceeding the limits established by the European Commission were found in species living in close contact with bottom sediments: deepwater rose shrimp (Parapenaeus longirostris), blue and red shrimp (Aristeus antennatus) and Norway lobster (Nephrops norvegicus). For shrimps, the inter-individual variability observed was mostly related to the body size, indicating the accumulation of mercury with age. An estimation of the human intake of mercury associated to the consumption of the crustaceans sampled and its comparison with the Tolerable Weekly Intake are provided.

Total mercury levels in commercial fish species from Italian fishery and aquaculture.

Total mercury levels were measured in 42 commercial fish species caught off the Central Adriatic and Tyrrhenian coasts of Italy and in 6 aquaculture species. The study on wild fish covered species differing in living habitat and trophic level. The study on farmed fish covered marine and freshwater species from intensive and extensive aquaculture and their feed. Mercury levels were analysed by thermal decomposition-amalgamation-atomic absorption spectrophotometry. Total mercury concentrations in the muscle of wild fish showed a high variability among species (0.025-2.20 mg kg-1 wet weight). The lowest levels were detected in low trophic-level demersal and pelagic-neritic fish and in young individuals of high trophic-level species. Levels exceeding the European Commission limits were found in large-size specimens of high trophic-level pelagic and demersal species. Fish from intensive farming showed low levels of total mercury (0.008-0.251 mg kg-1). Fish from extensive rearing showed variable contamination levels, depending on the area of provenience. An estimation of the human intake of mercury associated to the consumption of the studied fish and its comparison with the tolerable weekly intake is provided.

Analysis of fatty acids in 12 Mediterranean fish species: advantages and limitations of a new GC-FID/GC-MS based technique.

When fatty acids in fish are analyzed, results in percentage form (profile analysis) are mostly reported. However, the much more useful results expressed as mg/100 g (absolute analysis) is the main information required. Absolute methods based on calibration curves are of good accuracy but with a high degree of complexity if applied to a great number of analytes. Procedures based on the sequence profile analysis-total FA determination-absolute analysis may be suitable for routine use, but suffer from a number of uncertainties that have never been really resolved. These uncertainties are mainly related to the profile analysis. In fact, most profile analyses reported in the literature disagree about the number and type of fatty acids monitored as well as about the total percentage to assign to their sum so leading to possible inaccuracies; in addition the instrumental response factor for all FAME (fatty acid methyl esters) is often considered as a constant, but this is not exactly true. In this work, a set of 24 fatty acids was selected and studied on 12 fish species in the Mediterranean area (variable in lipid content and month of sampling): in our results, and in these species, this set constitutes, on average, 90 ± 3 % of the total fatty acid content. Moreover the error derived from the assumption of a unique response factor was investigated. Two different detection techniques (GC-FID and GC-MS) together with two capillary columns (different in length and polarity) were used in order to acquire complementary data on the same sample. With the protocol here proposed absolute analyses on the 12 cited species are easily achievable by the total FA determination procedure. The accuracy of this approach is good in general, but in some cases (DHA for example) is lower than the accuracy of calibration-based methods. The differences were evaluated on a case by case basis.

New trends in the seafood market. Sutchi catfish (Pangasius hypophthalmus) fillets from Vietnam: Nutritional quality and safety aspects.

Sutchi catfish (Pangasius hypophthalmus) produced in the freshwater basins of Vietnam, available on the Italian market as frozen or thawed fillets, were studied for their nutritional quality and safety aspects. Proximate composition, mineral content, fatty acid profile, unsaponifiable components of the lipid fraction and drip loss during thawing at 5°C were determined on the fillets. Fillets were characterised by high moisture levels (80-85%) and low protein (12.6-15.6%) and lipid (1.1-3.0%) contents. Total lipids were characterised by low cholesterol levels (21-39mg/100g), high percentages of saturated fatty acids (41.1-47.8% of total fatty acid) and low percentages of polyunsaturated fatty acids (12.5-18.8% of total fatty acids), which were mainly represented by linoleic acid (44-59% of total polyunsaturated fatty acids). The mineral composition was characterised by a high sodium content (222-594mg/100g), probably partially due to the sodium tripolyphosphate (E 451) used to retain moisture. As regards safety aspects, the quality of the samples analysed was good, with low residue levels of mercury, organochlorine pesticides and polychlorinated biphenyls.