Validation of a fecal scoring scale in puppies during the weaning period.

In puppies weaning is a high risk period. Fecal changes are frequent and can be signs of infection by digestive pathogens (bacteria, viruses, parasites) and indicators of nutritional and environmental stress. The aim of this study was to define a pathological fecal score for weaning puppies, and to study the impact on that score of two intestinal viruses (canine parvovirus type 2 and canine coronavirus). For this, the quality of stools was evaluated on 154 puppies between 4 and 8 weeks of age (100 from small breeds and 54 from large breeds). The scoring was performed immediately after a spontaneous defecation based on a 13-point scale (from 1; liquid to 13; dry and hard feces). Fecal samples were frozen for further viral analysis. Each puppy was weighed once a week during the study period. The fecal score regarded as pathological was the highest score associated with a significant reduction in average daily gain (ADG). Fecal samples were checked by semi-quantitative PCR or RT-PCR for canine parvovirus type 2 and canine coronavirus identification, respectively. The quality of feces was affected by both age and breed size. In small breeds, the ADG was significantly reduced under a fecal score of 6 and 7 for puppies at 4-5 and 6-8 weeks of age, respectively. In large breeds, the ADG was significantly reduced under a fecal score of 5 whatever the age of the puppy. Whereas a high viral load of canine parvovirus type 2 significantly impacted feces quality, no effect was recorded for canine coronavirus. This study provides an objective threshold for evaluation of fecal quality in weaning puppies. It also emphasizes the importance to be given to age and breed size in that evaluation.

Age-related changes in biochemical and hematologic variables in Borzoi and Beagle puppies from birth to 8 weeks.

BACKGROUND: Nonspecific signs of illness and availability of only limited information about expected biochemical and hematologic values in puppies make formulation of a differential diagnosis challenging for veterinarians. OBJECTIVES: The objectives of this prospective study were to describe age-related changes in biochemical and hematologic variables in Borzoi and Beagle puppies from birth to 8 weeks and to compare results with adult reference intervals (RIs). METHODS: Biochemical and hematologic analytes in blood collected in heparin and EDTA from 31 clinically healthy puppies (21 Beagle and 10 Borzoi dogs) were measured or calculated each week from birth to 8 weeks of age. RIs were calculated using the parametric robust method after Box-Cox transformation. RESULTS: There were no significant differences between values of puppies and adults for ALT activity, glucose concentration, MCHC, WBC count, and platelet count. Total protein concentration, RBC count, and HCT were lower and creatine kinase activity was higher in puppies of all ages compared with these variables in adults. Compared with adult RI, in puppies urea concentration was higher at birth and week 1, creatinine concentration was lower at weeks 1-5, ALP activity was higher at all weeks except for weeks 4 and 6, hemoglobin concentration was lower at weeks 1-8, MCV was higher at birth and week 1, and reticulocyte count was higher at all weeks except week 1. Statistically, mixed models accounted for evaluation of the same puppies each week and in most cases demonstrated an effect of age and sometimes breed. Some reference intervals should be used with caution owing to potential outliers. The 90% confidence intervals exceeded those recommended by the International Federation of Clinical Chemistry. CONCLUSIONS: Clinicians should be aware of temporal changes in biochemical and hematologic values for puppies during the first 2 months of life to avoid misinterpretation of results.