RESUMO
Vaccine hesitancy is a well-known phenomenon whereby individuals in a population reject or delay being vaccinated despite having access to vaccine services. This phenomenon is especially problematic in the current context of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) pandemic because vaccine hesitancy can decrease vaccination rates. In Mexico, vaccine hesitancy has been less thoroughly studied than in other countries such as the United States despite its importance and the potential impact of overlooking the problem. Understanding and effectively tackling this problem requires a more in-depth analysis of the defining characteristics of vaccination hesitancy. For this purpose, the World Health Organization's (WHO) Vaccine Hesitancy Determinants Matrix (VHDM in this study) is highly useful. In the present study, a digital survey was conducted using Google Forms to assess the level of vaccine hesitancy in the Mexican population and the vaccine determinants of the VHDM model associated with the respondents' different levels of vaccine hesitancy. The sample consisted of 1,195 people divided into four levels of vaccine hesitancy based on their answers. Tests for association were performed, identifying an association between some determinants of the VHDM model and the levels of vaccine hesitancy. Based on the analysis of the survey results, areas of opportunity for addressing COVID-19 vaccine hesitancy in Mexico were found and discussed; these included the importance of studying vaccine hesitancy as a complex and changing gradient, the high connectivity of people with high level of vaccine hesitancy to networks of people with vaccine hesitancy, the relatively high trust in physicians at all levels of vaccine hesitancy, the low trust in government authorities at high and moderate levels of vaccine hesitancy, and the strong association of the fear of suffering side effects and knowing people who have suffered them with the level of vaccine hesitancy.
RESUMO
Hybridization events are frequently demonstrated in natural butterfly populations. One interesting butterfly complex species is the Enantia jethys complex that has been studied for over a century; many debates exist regarding the species composition of this complex. Currently, three species that live sympatrically in the Gulf slope of Mexico (Enantia jethys, E. mazai, and E. albania) are recognized in this complex (based on morphological and molecular studies). Where these species live in sympatry, some cases of interspecific mating have been observed, suggesting hybridization events. Considering this, we employed a multilocus approach (analyses of mitochondrial and nuclear sequences: COI, RpS5, and Wg; and nuclear dominant markers: inter-simple sequence repeat (ISSRs) to study hybridization in sympatric populations from Veracruz, Mexico. Genetic diversity parameters were determined for all molecular markers, and species identification was assessed by different methods such as analyses of molecular variance (AMOVA), clustering, principal coordinate analysis (PCoA), gene flow, and PhiPT parameters. ISSR molecular markers were used for a more profound study of hybridization process. Although species of the Enantia jethys complex have a low dispersal capacity, we observed high genetic diversity, probably reflecting a high density of individuals locally. ISSR markers provided evidence of a contemporary hybridization process, detecting a high number of hybrids (from 17% to 53%) with significant differences in genetic diversity. Furthermore, a directional pattern of hybridization was observed from E. albania to other species. Phylogenetic study through DNA sequencing confirmed the existence of three clades corresponding to the three species previously recognized by morphological and molecular studies. This study underlines the importance of assessing hybridization in evolutionary studies, by tracing the lineage separation process that leads to the origin of new species. Our research demonstrates that hybridization processes have a high occurrence in natural populations.
Assuntos
DNA Mitocondrial/genética , Evolução Molecular , Variação Genética , Lepidópteros/genética , Animais , MéxicoRESUMO
CONTEXT AND OBJECTIVE: We used the wing somatic assay in Drosophila melanogaster to test the hypothesis that two phytoextracts from Cecropia obtusifolia Bertol (Cecropiaceae) and Equisetum myriochaetum Schlecht. et Cham (Equisetaceae), which are used in folk medicine to treat type 2 diabetes mellitus, could detoxify the mutagen hydrogen peroxide. MATERIALS AND METHODS: Third instar larvae from standard (ST) and high-bioactivation (HB) crosses were chronically exposed to different concentrations of the phytoextracts. Hydrogen peroxide was used to induce oxidative stress and was chronically tested in both crosses. Catalase activity was measured in larvae of both strains 48 h after treatment with hydrogen peroxide. A pretreatment protocol was devised to test the antimutagenic potency of the medicinal extracts. RESULTS: The present study showed that neither of the phytoextracts were genotoxic in Drosophila. Interestingly, the antioxidant enzyme activity levels were different between the larvae. Hydrogen peroxide resulted in a significant genotoxic effect in the ST cross, whereas a detoxification of hydrogen peroxide was found in the HB cross. Thus, catalase was stimulated in the HB cross, which was indicative of a cellular defense mechanism mounted against a xenobiotic hazard. We found that the percentage of inhibition of spots produced by E. myriochaetum was much higher than that induced by Cecropia obtusifolia. DISCUSSION AND CONCLUSIONS: These results are in agreement with the uses of these phytoextracts in traditional medicine. Indeed, the lack of genotoxicity and the antimutagenic activity observed for both phytoextracts validates their use as a therapeutic modality to treat diabetic patients. Moreover, these extracts are suitable for consumption as teas and/or phytomedicines.