RESUMO
Lactobacilli have been associated with dental caries for over a century. Here, we review the pertinent literature along with findings from our own study to formulate a working hypothesis about the natural history and role of lactobacilli. Unlike most indigenous microbes that stably colonize a host, lactobacilli appear to be planktonic, opportunistic settlers that can gather and multiply only in certain restrictive niches of the host, at least within the oral cavity. We postulate that the following essential requirements are necessary for sustained colonization of lactobacilli in humans: 1) a stagnant, retentive niche that is mostly anaerobic; 2) a low pH milieu; and 3) ready access to carbohydrates. Three sites on the human body meet these specifications: caries lesions, the stomach, and the vagina. Only a handful of Lactobacillus species is found in caries lesions, but they are largely absent in caries-free children. Lactobacilli present in caries lesions represent both a major contributor to caries progression and a major reservoir to the gastrointestinal (GI) tract. We extend the assertion from other investigators that lactobacilli found in the GI tract originate in the oral cavity by proposing that lactobacilli in the oral cavity arise from caries lesions. This, in turn, leads us to reflect on the health implications of the lactobacilli in the mouth and downstream GI and to ponder whether these or any of the Lactobacillus species are truly indigenous to the human GI tract or the oral cavity.
Assuntos
Cárie Dentária/microbiologia , Lactobacillus/fisiologia , Boca/microbiologia , Anaerobiose , Progressão da Doença , Trato Gastrointestinal/microbiologia , Humanos , Concentração de Íons de Hidrogênio , Lactobacillus/classificação , Streptococcus mutans/fisiologia , Sacarose/metabolismoRESUMO
We propose a new classification of severe early childhood caries (S-ECC): hypoplasia-associated severe early childhood caries (HAS-ECC). This form of caries affects mostly young children living at or below poverty, characterized by structurally damaged primary teeth that are particularly vulnerable to dental caries. These predisposing developmental dental defects are mainly permutations of enamel hypoplasia (EHP). Anthropologists and dental researchers consider EHP an indicator for infant and maternal stresses including malnutrition, a variety of illnesses, and adverse birthing conditions. Differentiation of HAS-ECC from other forms of early childhood caries is warranted because of its distinct etiology, clinical presentation, and eventual management. Defining HAS-ECC has important clinical implications: Therapies that control or prevent other types of caries are likely to be less effective with HAS-ECC because the structural integrity of the teeth is compromised prior to their emergence into the oral cavity. By the time these children present to the dentist, the treatment options often become limited to surgical management under general anesthesia. To prevent HAS-ECC, dentists must partner with other health providers to develop interventions that begin with pregnant mothers, with the aim of eliminating or ameliorating the covariates accompanying poverty, including better pre- and post-natal care and nutrition.
Assuntos
Cárie Dentária/classificação , Cárie Dentária/etiologia , Hipoplasia do Esmalte Dentário/complicações , Hipoplasia do Esmalte Dentário/embriologia , Terminologia como Assunto , Amelogênese , Alimentação com Mamadeira/efeitos adversos , Pré-Escolar , Índice CPO , Cárie Dentária/microbiologia , Suscetibilidade à Cárie Dentária , Dieta Cariogênica , Feminino , Humanos , Grupos Minoritários , Pobreza , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Fatores de Risco , Streptococcus mutans/fisiologia , Dente DecíduoRESUMO
Several methods for determining the diversity of Lactobacillus spp were evaluated with the purpose of developing a realistic approach for further studies. The patient population was comprised of young children with an oral disease called severe early childhood caries. The ultimate goal of these studies was to ascertain the role of lactobacilli in the caries process. To accomplish that goal, we evaluated several methods and approaches for determining diversity including AP-PCR, chromosomal DNA fingerprinting, denaturing gradient gel electrophoresis, and 16S rRNA gene sequencing. Central to these methods was the gathering and screening of isolates from cultivation medium. Using various estimates of diversity, we addressed the question as to how many isolates represent the overall diversity and how cultivation compares to non-cultivation techniques. Finally, we proposed a working approach for achieving the goals outlined framed by both practical constraints in terms of time, effort and efficacy while yielding a reliable outcome.
Assuntos
Variação Genética , Lactobacillus/classificação , Lactobacillus/genética , Boca/microbiologia , Pré-Escolar , Análise por Conglomerados , Impressões Digitais de DNA/métodos , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Cárie Dentária/microbiologia , Eletroforese em Gel de Campo Pulsado , Eletroforese em Gel de Poliacrilamida , Feminino , Genótipo , Humanos , Lactobacillus/isolamento & purificação , Masculino , Desnaturação de Ácido Nucleico , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
As modern humans (Homo sapiens) migrated out of Africa to different parts of the world, their obligate indigenous bacterial biota accompanied them. As both evolved, the accumulations of mutations in their DNA can reveal their phylogenies. Here, we describe the evolutionary history of an indigenous bacteria, Streptococcus mutans, from the oral cavity. Using several genetic markers, four distinct clusters of S. mutans genetic traits coincide with individuals of distinct geographic or racial groups comprised of two African clades and an Asian and a Caucasian clade. The evolutionary lineage of S. mutans is in agreement with anthropological artifacts marking the trail of human migrations.
Assuntos
DNA Bacteriano/genética , Emigração e Imigração , Evolução Molecular , Boca/microbiologia , Streptococcus mutans/genética , Análise por Conglomerados , Humanos , FilogeniaRESUMO
The aim of this study was to examine the colonization of Streptococcus mutans and Streptococcus sanguinis in the oral cavity and the association with severe early childhood caries (S-ECC). Saliva and plaque samples were collected from 14 S-ECC children and 8 caries-free (CF) children. All S-ECC children were S. mutans positive; 100% of CF children and 93% of S-ECC children were S. sanguinis positive. The children's caries severity was positively correlated with levels of S. mutans (p < 0.001), total oral streptococci (p < 0.01), total cultivable oral bacteria (p < 0.05), and children's age (p < 0.05). Logistic regression analysis showed that the interaction of S. sanguinis with S. mutans was a significant factor associated with the caries status in children, suggesting that the relative levels of these two microorganisms in the oral cavity play an important role in caries development.
Assuntos
Índice CPO , Cárie Dentária/microbiologia , Streptococcus mutans/fisiologia , Streptococcus/fisiologia , Criança , Pré-Escolar , Contagem de Colônia Microbiana , Placa Dentária/microbiologia , Feminino , Humanos , Lactobacillus/fisiologia , Masculino , Saliva/microbiologia , Streptococcus/classificação , Streptococcus sobrinus/fisiologiaRESUMO
For nearly a century, lactobacilli (LB) in the oral cavity have been generally associated with dental caries. Here, we characterized the LB isolated from the saliva of 6 women with active caries using genetic-based taxonomical identification methods. From each subject, 30 isolates growing on Rogosa medium and presumed to be LB were analyzed. Of the 180 isolates, 176 were further characterized by biotyping, DNA melting points, DNA chromosomal fingerprinting, genotyping, and phylogenetic cluster assessment. We found a total of 30 unique genotypes of LB in the saliva of caries-active women, with each woman harboring between 2 and 8 distinct genotypes. Although Lactobacillus vaginalis, L. fermentum, and L. salivarius were found in 4 of 6 of the subjects, results from other studies using comparable methods show an entirely different array of LB associated with caries. These collective observations lead us to surmise that LB associated with dental caries are likely exogenous and opportunistic colonizers, arising from food or other reservoirs outside the oral cavity.
Assuntos
Cárie Dentária/microbiologia , Lactobacillus/genética , Boca/microbiologia , Saliva/microbiologia , Adolescente , Adulto , Impressões Digitais de DNA/métodos , DNA Bacteriano/análise , DNA Bacteriano/genética , Feminino , Humanos , Técnicas de Amplificação de Ácido Nucleico/métodos , FenótipoRESUMO
The determination of the composition of the microbial community in the oral cavity is usually based on cultivation methods; however, nearly half of the bacteria in the saliva and the dental plaque are not cultivable. In this study, we evaluated the difference in oral microbial diversity between children with severe early-childhood caries (S-ECC) and caries-free (CF) controls by means of a cultivation-independent approach called denaturing gradient gel electrophoresis (DGGE). Pooled dental plaque samples were collected from 20 children aged 2 to 8 years. Total microbial genomic DNA was isolated from those subjects, and a portion of the 16S rRNA gene locus was PCR amplified by using universal primers. We observed that the mean species richness of the bacterial population was greater in the CF children (n = 12) (42 +/- 3.7) than in the S-ECC children (n = 8) (35 +/- 4.3); the difference was statistically significant (P = 0.005). The overall diversity of plaque samples as measured by the Shannon index was 3.5 for the S-ECC group and 3.7 for the CF group (P = 0.004). Differences in DGGE profiles were distinguished on the basis of a cluster analysis. Sequence analysis of excised DGGE bands consisted of 2.7 phylotypes, on average. After adjusting for the number of observed bands, we estimated that the S-ECC group exhibited 94.5 total phylotypes and that the CF group exhibited 113.4. These results suggest that the microbial diversity and complexity of the microbial biota in dental plaque are significantly less in S-ECC children than in CF children.
Assuntos
Técnicas de Tipagem Bacteriana , DNA Bacteriano/análise , Cárie Dentária/microbiologia , Placa Dentária/microbiologia , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Criança , Pré-Escolar , Análise por Conglomerados , DNA Bacteriano/isolamento & purificação , Eletroforese em Gel de Poliacrilamida/métodos , Feminino , Bactérias Gram-Negativas/classificação , Bactérias Gram-Negativas/genética , Bactérias Gram-Positivas/classificação , Bactérias Gram-Positivas/genética , Humanos , Masculino , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
S. mutans plays a key role in dental caries. The extent to which perinatal events influence the acquisition of S. mutans is unclear. We hypothesized that several maternal factors, including the mode of delivery, influence the initial acquisition of S. mutans in infants. A prospective cohort study was conducted in 156 mother-infant pairs. The study found that maternal gestational age (p = 0.04), S. mutans level (p = 0.02), caries score (p = 0.02), sexually transmitted disease (STD) infection experience (p = 0.01), and family income (p = 0.03) had significant effects on the acquisition of S. mutans. Among infants who became infected, those delivered by Caesarean section acquired S. mutans 11.7 mos earlier than did vaginally delivered infants (p = 0.038). C-section infants harbored a single genotype of S. mutans that was identical to that of their mothers (100% fidelity). Analysis of the data demonstrated the possible perinatal influences on infants' acquisition of a member of the cariogenic microbiota, and its potential effect on caries outcome.
Assuntos
Cesárea , Transmissão Vertical de Doenças Infecciosas , Boca/microbiologia , Infecções Estreptocócicas/transmissão , Streptococcus mutans/isolamento & purificação , Análise de Variância , Cárie Dentária , Feminino , Genótipo , Idade Gestacional , Humanos , Lactente , Modelos Logísticos , Masculino , Bem-Estar Materno , Gravidez , Saliva/microbiologia , Classe Social , Estatísticas não Paramétricas , Streptococcus mutans/genéticaRESUMO
Polymicrobial biofilms in the human oral cavity exhibit marked diversity. PCR-based denaturing gradient gel electrophoresis (PCR-DGGE) surveys microbial diversity by displaying PCR-generated 16S rDNA fragments that migrate at different distances, reflecting the differences in the base-pair (i.e., % G+C) composition of the fragment. This study examined DGGE-generated diversity profiles of cultivable bacteria from individuals with different caries status. Initially, we developed a set of PCR-DGGE running conditions appropriate to oral bacteria. Next, we assessed migration standards from known oral bacterial reference strains. To test the methods, we profiled 20 bacterial saliva samples cultivated from young adults. The study produced a battery of species-specific 16S rDNA amplicons that could be used as a migration distance standard necessary for computer-assisted profile analysis. From the clinical samples, we found a significantly greater diversity of oral microbes in caries-free individuals compared with caries-active individuals (P = 0.01). These findings suggest thtat a portion of oral microbiota of caries-active individuals may be absent, suppressed, or replaced.
Assuntos
Bactérias/classificação , Boca/microbiologia , Actinomyces/classificação , Adolescente , Adulto , Bactérias/genética , Pareamento de Bases/genética , Biofilmes , DNA Ribossômico/análise , Cárie Dentária/microbiologia , Eletroforese em Gel de Poliacrilamida , Feminino , Fusobacterium/classificação , Variação Genética/genética , Humanos , Lactobacillus/classificação , Reação em Cadeia da Polimerase , Porphyromonas gingivalis/classificação , RNA Ribossômico 16S/análise , Especificidade da Espécie , Staphylococcus/classificação , Streptococcus/classificaçãoRESUMO
Actinomyces naeslundii genospecies 2 (gsp-2) are members of the autochthonous oral flora. Chromosomal DNA fingerprinting (CDF) with SmaI revealed extensive genetic diversity among A. naeslundii gsp-2 strains within individual mothers and children. There was a low prevalence of genotype match among A. naeslundii gsp-2 strains between all mother and child pairs.
Assuntos
Actinomyces/genética , Genes Bacterianos , Variação Genética , Adulto , Criança , Impressões Digitais de DNA , Feminino , Genótipo , Humanos , Mães , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
In a randomized clinical trial, we evaluated the effect of a 10% chlorhexidine varnish (Chlorzoin) on the mother-child transmission of Streptococcus mutans and on subsequent caries experience. Chlorhexidine (n = 38) or a placebo varnish (n = 37) was applied to the dentitions of 75 mothers at a time when their first babies were about 6 months old (approximate time of first tooth emergence). Three more applications at weekly intervals and subsequent applications at 6-month intervals followed the initial application. The mother-child pairs were followed up until the child's fourth birthday. Maternal salivary S. mutans levels in the treatment group remained significantly lower (p < 0.05) compared to the control group up to 12 months after the initial application. However, this intervention did not significantly alter the S. mutans colonization in children or the caries increment in either the mother or the child.
Assuntos
Anti-Infecciosos Locais/uso terapêutico , Cariostáticos/uso terapêutico , Clorexidina/uso terapêutico , Cárie Dentária/prevenção & controle , Etanol/uso terapêutico , Transmissão Vertical de Doenças Infecciosas/prevenção & controle , Mães , Poliuretanos/uso terapêutico , Infecções Estreptocócicas/transmissão , Adolescente , Adulto , Anti-Infecciosos Locais/administração & dosagem , Cariostáticos/administração & dosagem , Pré-Escolar , Clorexidina/administração & dosagem , Cárie Dentária/microbiologia , Etanol/administração & dosagem , Feminino , Humanos , Lactente , Laca , Masculino , Pessoa de Meia-Idade , Poliuretanos/administração & dosagem , Saliva/microbiologia , Infecções Estreptocócicas/prevenção & controle , Streptococcus mutans/isolamento & purificação , Falha de TratamentoRESUMO
Actinomyces are difficult to identify using serological and biochemical methods but genotyping is an efficient and reliable means of bacterial characterization and can be used to determine clonal identity. The purpose here was to genotype 13 American type culture collection (ATCC) reference strains representing six different oral Actinomyces spp. by using chromosomal DNA fingerprinting (CDF), arbitrarily primed-polymerase chain reaction (AP-PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In CDF analysis, BamHI, BstEII and SmaI yielded digestion patterns revealing characteristic differences among the known Actinomyces spp., with SmaI demonstrating optimal resolution. Amplicons generated by AP-PCR with primer OPB-07 displayed banding patterns that permitted discrimination of all Actinomyces strains tested. PCR-RFLP with MnlI digests generated fragment patterns that also characterized the reference strains. Collectively, genotypic profiles generated by CDF, AP-PCR and PCR-RFLP permitted differentiation of all 13 ATCC Actinomyces strains. SmaI CDF analysis of 18 clinical isolates of catalase-positive A. naeslundii genospecies 2 revealed extensive genetic diversity among these strains. These molecular approaches may be useful in determining genetic diversity within oral Actinomyces populations and fidelity of Actinomyces transmission between mother and child.
Assuntos
Actinomyces/genética , Boca/microbiologia , Actinomyces/classificação , Catalase/genética , Pré-Escolar , Cromossomos Bacterianos/genética , Impressões Digitais de DNA , Enzimas de Restrição do DNA , DNA Bacteriano/genética , Desoxirribonuclease BamHI , Desoxirribonucleases de Sítio Específico do Tipo II , Feminino , Variação Genética , Genótipo , Humanos , Técnicas de Amplificação de Ácido Nucleico , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Saliva/microbiologiaRESUMO
Dental caries is an infectious disease of bacterial origin. The use of antimicrobial agents to reduce or eliminate the bacteria associated with caries follows the approach used to combat other infectious diseases of humans. Unfortunately, only a few dozen studies have sufficient resolving power to make inferences as to the anticaries efficacy of the antimicrobial approach to caries management. Here, we comment on the findings of the RTI/UNC review concerning antimicrobials, discuss additional findings not covered in that review, and make recommendations based upon both the available literature and from our own experience. Even though the studies published thus far are inconclusive or lack sufficient demonstration of efficacy to recommend a specific approach involving antimicrobial agents in routine clinical practice, several pieces of information from these studies suggest future avenues of investigation.
Assuntos
Anti-Infecciosos Locais/administração & dosagem , Cárie Dentária/tratamento farmacológico , Adolescente , Antibacterianos/administração & dosagem , Criança , Clorexidina/administração & dosagem , Bases de Dados Bibliográficas , Cárie Dentária/microbiologia , Humanos , Canamicina/administração & dosagem , Ensaios Clínicos Controlados Aleatórios como Assunto , Streptococcus mutans/efeitos dos fármacos , Vancomicina/administração & dosagemRESUMO
Approximately 5% of strains of Streptococcus mutans contain plasmid DNA. Strain UA140 harbors a 5.6-kb cryptic plasmid, pUA140, with an overall G+C content of 32.7%. Five open reading frames (ORF), encoding peptides of larger than 100 amino acid residues, were initially designated as ORF1 to ORF5. These five ORFs were located on the same strand of pUA140. ORF1 (258 amino acids) resembled a replication protein, Rep. Upstream of the putative Rep gene, a double-stranded origin for plasmid replication that showed strong similarity to those of a number of plasmids in the pT181 family was identified. Further upstream was a region constituting the single-stranded origin of replication. A single-stranded DNA intermediate was detected during plasmid replication. Taken together, these results suggest that pUA140 replicated by the rolling circle replication mechanism but exhibited several characteristics that differ from those of other members of the pT181 plasmid family.
Assuntos
Genes Bacterianos/genética , Plasmídeos/genética , Streptococcus mutans/genética , Motivos de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Composição de Bases , Sequência de Bases , Southern Blotting , Replicação do DNA/genética , DNA de Cadeia Simples/biossíntese , DNA de Cadeia Simples/genética , DNA de Cadeia Simples/metabolismo , Bases de Dados Genéticas , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Fases de Leitura Aberta/genética , Plasmídeos/biossíntese , Plasmídeos/metabolismo , Subunidades Proteicas , Origem de Replicação/genética , Análise de Sequência de DNA , Homologia de SequênciaRESUMO
Streptococcus sanguis comprises a heterogeneous group of oral streptococci indigenous to the oral cavity of humans. A total of 289 isolates from an infant population (n=37) were tentatively identified as S. sanguis on the basis of the distinctive colony morphology as shown on MM10-sucrose non-selective medium. These isolates were divided into four biovars of S. sanguis as determined by an extended panel of biochemical attributes. Chromosomal DNA was extracted from each isolate, and an AP-PCR fingerprint profile was obtained to allow study of the diversity within and among the infants. In this study, all four biovars of S. sanguis were detected in the infants. A wide genotypic diversity of S. sanguis was observed among these isolates; on average, each infant harbored 2.7 unique amplitypes as shown by the AP-PCR fingerprints. To explore the phylogenic relationship among these S. sanguis isolates, 20 strains representing the four biovars were selected at random for sequencing of their 16S rDNA and 16S-23S rDNA intergenic spacer chromosomal loci. Two major sequence patterns were identified within the 16S rDNA sequences. A phylogenic analysis showed that members from each of the four biovars of S. sanguis bore close relationship with the type-strain ATCC 10556 sequence, and that all of the isolates representing the four biovars could be clustered into two main phylotypes. The biovars were distributed throughout the phylotypes, indicating no correlation between the genetic and phenotypic groupings.
Assuntos
Boca/microbiologia , Streptococcus sanguis/genética , Técnicas de Tipagem Bacteriana , Mapeamento Cromossômico , Cromossomos Bacterianos/genética , Estudos de Coortes , Meios de Cultura , Impressões Digitais de DNA , DNA Bacteriano/análise , DNA Intergênico/genética , Variação Genética , Genótipo , Humanos , Lactente , Recém-Nascido , Estudos Longitudinais , Fenótipo , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , RNA Ribossômico 23S/genética , Análise de Sequência de DNA , Estatística como Assunto , Streptococcus sanguis/classificaçãoRESUMO
Streptococcus mutans strains were isolated from cohorts of Brazilian nursery school children and genotyped by arbitrarily primed PCR and restriction fragment length polymorphism analysis. Of 24 children with two to five S. mutans isolates, 29% carried two or more genotypes. The presence of matching genotypes of S. mutans among children attending one nursery suggests horizontal transmission.
Assuntos
Transmissão de Doença Infecciosa , Variação Genética , Escolas Maternais , Infecções Estreptocócicas/transmissão , Streptococcus mutans/classificação , Brasil , Pré-Escolar , DNA Bacteriano/análise , Genótipo , Humanos , Lactente , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Infecções Estreptocócicas/microbiologia , Streptococcus mutans/genéticaRESUMO
Routine identification of Streptococcus mutans and Streptococcus sobrinus is generally based upon growth on various selective media, colony morphology and biochemical characteristics. We examined various approaches of differentiating these two species through a combination of the conventional phenotypic methodology with chromosomal DNA fingerprint (CDF) and arbitrarily primed polymerase chain reaction (AP-PCR) methods. Initially, ten ATCC type strains and 20 randomly selected clinical isolates of mutans streptococci (MS) were characterized and grouped into two major types based on patterns generated by the CDF using HaeIII digestion. The CDF's patterns with restriction fragments equal to or greater than 6.6 kb were defined as the CDF-1 group. The CDF's patterns with restriction fragments less than 6.6 kb were defined as the CDF-2 group. Both groups were then examined for biotype, serotype, and composition of DNA via thermal denaturation. AP-PCR was applied and evaluated for the capability of delineating S. mutans from S. sobrinus strains. Results of this study showed that all CDF-1 strains fit within a G+C range of 36.2% to 42.2%, whereas the CDF-2 strains had a G+C range of 45.8% to 47.0%. The serotyping assay exhibited 100% sensitivity, 90% specificity and 86.7% agreement with the CDF. The biotyping assay presented the poorest specificity (38.5%), indicating the highest variability. The capability of AP-PCR in differentiation of S. mutans from S. sobrinus was comparable to the CDF method, suggesting that either of these two approaches can and may serve as a viable alternative method to serotyping or biotyping of MS.
Assuntos
Streptococcus mutans/classificação , Streptococcus sobrinus/classificação , Análise de Variância , Técnicas de Tipagem Bacteriana , Cromossomos Bacterianos/genética , Citosina/análise , Impressões Digitais de DNA , Primers do DNA , DNA Bacteriano/análise , DNA Bacteriano/genética , Desoxirribonucleases de Sítio Específico do Tipo II , Genótipo , Guanina/análise , Humanos , Fenótipo , Reação em Cadeia da Polimerase , Mapeamento por Restrição , Sensibilidade e Especificidade , Sorotipagem , Estatística como Assunto , Estatísticas não Paramétricas , Streptococcus mutans/genética , Streptococcus sobrinus/genéticaRESUMO
The lantibiotic mutacin II, produced by Streptococcus mutans T8, is a ribosomally synthesized peptide antibiotic that contains thioether amino acids such as lanthionine and methyllanthionine as a result of post-translational modifications. The mutacin II leader peptide sequence shares a number of identical amino acid residues with class AII lantibiotic leader peptides. To study the role of these conservative residues in the production of active antimicrobial mutacin, 15 mutations were generated by site-directed mutagenesis. The effects of these substitutions vary from no effect to complete block-out. Mutations G-1A, G-2A, I-4D, and L-7K completely blocked the production of mature mutacin. Other mutations (I-4V, L-7M, E-8D, S-11T/A, V-12I/A, and E-13D) had no detectable effect on mutacin production. The changes of Glu-8 to Lys, Val-12 to Leu, Glu-13 to Lys reduced the mutacin production level to about 75%, 50%, and 10% of the wild-type, respectively. Thus, our data indicated that some of these conserved residues are essential for the mutacin biosynthesis, whereas others are important for optimal biosynthesis rates.
Assuntos
Substituição de Aminoácidos , Bacteriocinas/biossíntese , Sinais Direcionadores de Proteínas , Streptococcus mutans/metabolismo , Sequência de Aminoácidos , Bacteriocinas/química , Bacteriocinas/genética , Bacteriocinas/farmacologia , Genes Bacterianos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Precursores de Proteínas/biossíntese , Sinais Direcionadores de Proteínas/genética , Streptococcus mutans/genéticaRESUMO
Strains of Streptococcus mutans produce at least three mutacins, I, II, and III. Mutacin II is a member of subgroup AII in the lantibiotic family of bacteriocins, and mutacins I and III belong to subgroup AI in the lantibiotic family. In this report, we characterize two mutacins produced by UA140, a group I strain of S. mutans. One is identical to the lantibiotic mutacin I produced by strain CH43 (F. Qi et al., Appl. Environ. Microbiol. 66:3221-3229, 2000); the other is a nonlantibiotic bacteriocin, which we named mutacin IV. Mutacin IV belongs to the two-peptide, nonlantibiotic family of bacteriocins produced by gram-positive bacteria. Peptide A, encoded by gene nlmA, is 44 amino acids (aa) in size and has a molecular mass of 4,169 Da; peptide B, encoded by nlmB, is 49 aa in size and has a molecular mass of 4,826 Da. Both peptides derive from prepeptides with glycines at positions -2 and -1 relative to the processing site. Production of mutacins I and IV by UA140 appears to be regulated by different mechanisms under different physiological conditions. The significance of producing two mutacins by one strain under different conditions and the implication of this property in terms of the ecology of S. mutans in the oral cavity are discussed.
Assuntos
Antibacterianos/biossíntese , Bacteriocinas/biossíntese , Peptídeos , Streptococcus mutans/metabolismo , Actinomycetales/efeitos dos fármacos , Sequência de Aminoácidos , Bacteriocinas/química , Bacteriocinas/genética , Bacteriocinas/farmacologia , Cromatografia Líquida de Alta Pressão , Cárie Dentária/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Alinhamento de Sequência , Análise de Sequência de DNA , Infecções Estreptocócicas/microbiologia , Streptococcus/efeitos dos fármacosRESUMO
Of the infectious diseases that affect humans, dental caries may be the most prevalent. Pediatric primary medical care providers are usually the first health care providers to examine the oral cavity of a child and so need to be able to recognize suspicious dental lesions. This article provides information on recognizing caries and reviews the epidemiology, pathogenesis, and treatment of caries and odontogenic infections.