Esophageal atresia in infants with very low birth weight.

Infants with esophageal atresia (EA), with or without a tracheoesophageal fistula (TEF) frequently are of low birth weight. With advances in neonatal, respiratory, surgical, and anesthetic care, more infants with very low birth weight (VLBW; birth weight less than 1.5 kg) are surviving. The therapy of the VLBW neonate with EA is not longer automatically staged. Primary or delayed primary anastomosis can be performed safely if the patient is stable. This report will review the epidemiology, pathophysiology, treatment, and prognosis of EA in VLBW infants. The authors present their own experience in dealing with the VLBW with EA and review the world literature.

Resuscitation of the surgical neonate.

This article reviews the general principles of resuscitation of a neonate with a surgical disorder. These principles include careful attention to critical management issues of the airway, ventilation, perfusion, nutritional support and thermogenesis. In addition, a variety of surgical disorders in the neonatal period are discussed. For each disorder, early recognition of presentation patterns, efficient work-up strategy, initial management steps, and basic surgical principles are stressed. This framework can provide a basis for successful cooperation between the neonatologist and the pediatric surgeon for the initial care of the neonate requiring surgery.

Use of the aortic homograft in the reconstruction of complex tracheobronchial tree injuries.

BACKGROUND/PURPOSE: The repair of complex tracheobronchial tree injuries often is associated with complications including stenosis and excessive granulation tissue formation. Patch reconstruction using pericardium or synthetic material decreases the incidence of stenosis. The authors report on the use of the ascending aorta cryopreserved homograft in reconstructing the tracheobronchial tree of three children. RESULTS: A 10 year old had a through-and-through gun shot wound to the left main stem bronchus with cartilaginous loss. A 12 year old double lung transplant recipient had a breakdown of one bronchial anastomosis after stent placement. A 1 year old had an acquired tracheoesophageal fistula secondary to a long standing foreign body. In all three patients, the aortic patches were secured with interrupted sutures. All three had uneventful recoveries, and postoperative bronchoscopy showed the patches to be completely covered with epithelium without stenosis or granulation tissue. CONCLUSIONS: This is the first report of the use of the cryopreserved ascending aorta homograft in the reconstruction of the tracheobronchial tree. The homograft is strong yet malleable. It gets completely covered with epithelium and results in no stenosis because of the lack of tension. These characteristics make it an attractive alternative for the patch reconstruction of complex tracheobronchial tree injuries.

Presacral neuroenteric fistula presenting with meningitis and vaginal fistula: a case report.

Neuroenteric fistulas are rare congenital malformations arising from a persistent connection between the embryonic neural and intestinal tissues; they are extremely rare in the lumbosacral area. Neuroenteric fistulas should be suspected whenever children present with anaerobic meningitis in the absence of brain abscesses or respiratory infections. This patient is the first patient who presented with a presacral cyst that separately connected to the nervous system and the vagina. It is thought that the rape of this 12-year-old patient resulted in soilage of the spinal fluid through dual fistulae, causing anaerobic meningitis without an abscess.

A modification of the Bianchi intestinal lengthening procedure with a single anastomosis.

BACKGROUND/PURPOSE: Intestinal lengthening, as described by Bianchi, is the most commonly used method of gastrointestinal reconstruction for the therapy of short bowel syndrome. It divides the bowel in two longitudinal halves based on the bifurcated mesenteric blood supply, then reconnects the two halves in series with the rest of the small intestine. This requires three end-to-end narrow anastomoses in addition to the two longitudinal sutures lines. The authors describe a modification of the Bianchi procedure that lengthens the small intestine with a single wide end-to-end anastomosis thus avoiding two suture lines and their potential complications. METHODS: The anatomic principle used in this modification is the same as that described by Bianchi. The mesentery is separated based on the bifurcated vessels. With sequential firings of the stapler, the division of the intestine begins obliquely, proceeds longitudinally as in the Bianchi procedure, then ends obliquely at the other end of the intestine. This results in two divided segments that remain attached to the proximal and distal intestine and end in tapered staple lines. The two ends are sewn into a wide tapered anastomosis, reestablishing intestinal continuity with a single anastomosis instead of three. CONCLUSIONS: This simple modification, which we have used on two patients so far, decreases the number of anastomoses in an intestinal lengthening procedure from three narrow ones to one that is wider and tapered, thus reducing the risk of leaks and strictures. Because the end result is a lengthened intestinal segment similar to the Bianchi procedure, it should be equally effective in the therapy of short bowel syndrome.

Immunomodulation of pancreatic islet allografts in mice with CTLA4Ig secreting muscle cells.

In an effort to create a model of in vivo production of immunosuppressants, we have transfected C2C12 muscle cells (H-2k) with the cDNA for CTLA4Ig, a fusion protein that prevents the activation of T cells by blocking the costimulatory signal transduced by the T cell receptors CD28 and CTLA4. CTLA4Ig-secreting clones were cotransplanted with islets as composite grafts in the renal subcapsular space of diabetic mice. When the myoblasts were syngeneic to C3H/HeJ hosts (H-2k), there was a significant prolongation of survival of allogeneic C57Bl/6J (H-2b) islets from a mean 11.0 days to 31.7 days. When the graft was completely allogeneic (H-2k myoblasts and islets into H-2b recipients), there was no benefit in survival. A transient blockade of LFA-1 with the mAb M17 was synergistic in this combination: 8 out of 12 C57Bl/6J recipients achieved long-term acceptance. Systemic CTLA4Ig levels were detected up to 60 days after transplantation. In conclusion, we have shown that C2C12 muscle cells can be genetically engineered to secrete functional CTLA4Ig and that they can be used as a gene reservoir for the continuous in vivo production of CTLA4Ig to modulate the survival of islet cell allografts.

Outcome of cardiovascular collapse in pediatric blunt trauma.

STUDY OBJECTIVES: To determine the survival and functional outcome of pediatric blunt trauma victims demonstrating cardiovascular collapse, including pulseless cardiopulmonary arrest or severe hypotension, on initial presentation in an emergency department. DESIGN: Seven-year consecutive case-control series. SETTING: Level I trauma center and university teaching hospital. PARTICIPANTS: Two thousand one hundred twenty consecutive pediatric victims of blunt trauma less than 16 years old admitted to a Level I trauma center from August 1984 through December 1991 had a mortality of 5.2%. Thirty-eight patients (1.8%) demonstrated pulseless cardiac arrest or severe hypotension (systolic blood pressure of 50 mm Hg or less) on initial presentation in the ED. INTERVENTIONS: All patients received basic and advanced life support consistent with guidelines published by the American Heart Association, American Academy of Pediatrics, and American College of Surgeons. MEASUREMENTS AND MAIN RESULTS: Survival, functional outcome, and donor status were reviewed. Outcome of ED resuscitation (death or reanimation), post-ED destination (morgue, operating room, or pediatric ICU) length of hospitalization, functional outcome after hospital discharge, time to death (time from admission to ED to declaration of death), cause of death, total hospital costs, total hospital charges, and organ donation were reviewed. There were no functional survivors among 38 pediatric victims of blunt trauma who presented to the ED in pulseless cardiac arrest or with severe hypotension. Eleven of the 12 patients who were transferred to the pediatric ICU died; the single survivor demonstrated profound neurologic impairment six years after hospitalization. Six of these 12 patients were eligible potential donors and resulted in four multiorgan donors during the seven-year study. The mean hospital unreimbursed care for the 38 study patients was $3,514 per patient. CONCLUSION: No child who presented with pulseless cardiac arrest or severe hypotension following blunt trauma achieved functional survival. Reimbursed care for pediatric victims of blunt trauma demonstrating cardiovascular collapse is disproportionately poor compared with that for pediatric patients who maintain hemodynamic integrity in the ED. Half of all patients who were stabilized sufficiently for transfer to the pediatric ICU were eligible potential organ donors. Therefore aggressive resuscitation of these patients may be justified if organ donation is seriously contemplated and aggressively pursued.