RESUMO
Silkworms show high variability in silk quality and disease resistance. Attempts are on to combine the disease tolerance of multivoltine races and the silk quality of bivoltine races to generate new races with desirable phenotypic traits. We report the identification of a 26.5-kDa protein that is overexpressed in the gut juice of disease-resistant multivoltine races and that has anti-BmNPV activity. We have characterized this protein as a soluble NADH-oxidoreductase-like protein (BmNOX). Treatment of live BmNPV particles with BmNOX inhibited the capability of the viral particles to infect BmN cells in vitro.
Assuntos
Antivirais/metabolismo , Antivirais/farmacologia , Bombyx/enzimologia , Suco Gástrico/enzimologia , NADH NADPH Oxirredutases/metabolismo , NADH NADPH Oxirredutases/farmacologia , Animais , Antivirais/isolamento & purificação , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Peso Molecular , NADH NADPH Oxirredutases/isolamento & purificação , SolubilidadeRESUMO
The photoreceptive extreme tip of the wheat coleoptile exhibits intense green-yellow fluorescence under UV light, suggesting the presence of UV-absorbing materials. Fluorescence spectra of the intact coleoptile tip and tip homogenate showed the presence of the known photoreceptor pigments flavin and carotene, and a preponderance of phenolic compounds. Absorption spectra and fluorescence spectra of various phenolic compounds showed close overlap with the absorption and fluorescence spectra of the wheat coleoptile tip homogenate. Fluorescence spectra of several phenolic compounds showed close overlap with the absorption bands of flavin, carotene and pterine, suggesting possible energy transduction from phenols to these photoreceptors. Excitation of gentisic acid and ferulic acid with 340 nm light in the presence of flavin showed enhancement of flavin fluorescence in a concentration- and viscosity-dependent fashion, indicating fluorescence resonance energy transfer between them and riboflavin. Furthermore, several phenolic compounds tested generated superoxide anion on excitation at 340 nm, suggesting that superoxide-dependent signal cascades could operate in a polyphenol-mediated pathway. Phenolic compounds thus may act as accessory photoreceptors bringing about excitation energy transfer to the reactive photoreceptor molecules, or they may take over the function of the normal photoreceptor in genetic mutations lacking the system, or both processes may occur. The responses of plants to UV-B and UV-A light in mutants may be explained in terms of various phenolics acting as energy transducers in photoreceptor functioning.
Assuntos
Flavonoides/química , Luz , Fenóis/química , Cotilédone/química , Cotilédone/fisiologia , Cotilédone/efeitos da radiação , Transferência Ressonante de Energia de Fluorescência/métodos , Glicerol , Complexo de Proteínas do Centro de Reação Fotossintética/fisiologia , Complexo de Proteínas do Centro de Reação Fotossintética/efeitos da radiação , Polifenóis , Triticum/química , Triticum/fisiologia , Triticum/efeitos da radiaçãoRESUMO
Blue-light-induced photomorphogenesis is the sum total of a sequence of phenomena involving absorption of light by specific receptors, generation of a signal, processing transmembrane transport of signal, and the activation of a cascade of reactions in the cell interior. Though four blue-light receptors cryptochrome1, cryptochrome2, phototropin1, and phototropin2 have been identified, the signal transduction events associated with blue-light receptor activation are not understood. In this report, we demonstrate the generation and spatiotemporal distribution of H(2)O(2) in wheat coleoptile in response to blue light. Interception of the free-radical generation pathways dithiothreitol and propyl gallate rendered wheat coleoptile tips phototropically non-responsive. Unilateral application of H(2)O(2) onto the sub-apical region of a growing coleoptile brought about curvature in dark. Blue light also caused lipid peroxidation and augmented membrane rigidity of coleoptile cell membranes. We conclude that H(2)O(2) can act as a translocating second messenger that could bring about coleoptile curvature, and the signaling events may trigger Ca(2+) signaling cascades, changes in gene expression, and protein modifications.