First record of plastiglomerates, pyroplastics and plasticrusts along the beaches of Tamilnadu, Southeast coast of India.

Plastic litter affects coastal and marine ecosystems globally. This study represents the first record of pyroplastics and plasticrust in the beaches of Tamil Nadu, India. All samples were FTIR spectroscopically examined to confirm the polymer composition of the suspected plastics. The 16 plastic formations were found in TamilNadu, including six plastiglomerates nine pyroplastics and one plasticrust. Five types of polymers (PET, PP, PVC, PA, and PE) were found on the plastic matrices. The study also revealed that pyroplastics and plasticrust formed by degradation of plastics through weathering in the coastal environment. The present study also found that four types of marine fouling organisms such as oyster larvae, bryozoan, barnacle and polychaete worm were encrusted on the two pyroplastics. The emergence of these new forms of plastic raises concerns about their interactions with the environment and biota.

Spatiotemporal variations in marine macro-litter pollution along the shoreline of Koh Mun Nai, an uninhabited island in the Gulf of Thailand.

Despite a recent ambitious plan to improve waste management in Thailand, few studies have monitored the impact of these policies on beached marine litter. Here, we assessed weekly the amounts and composition of stranded macro-litter (≥2.5 cm) on five beaches from an uninhabited island in Thailand during one year. A total of 24,407 items (391.86 kg) yielded a mean abundance of 3.18 ± 11.39 items m-2 (52.75 ± 204.68 g m-2), with plastic being the most abundant marine litter (48% of the total number). The overall Clean Coast Index (30.1) classified the beaches as 'extremely dirty', with a Plastic Abundance Index of 9.8 ('very high abundance' of plastics). When assessing the seasonal rates of accumulation, we found a higher flux pre-monsoon (0.05 items m-2 d-1; 0.66 g m-2 d-1) than post-monsoon (0.01 items m-2 d-1; 0.35 g m-2 d-1). Using modeling of the local hydrodynamic conditions, we explored the potential sources of the pollution, and surprisingly found that the closest river appeared not to be the source. Our results denote that the distribution and typology of marine litter were representatives of household and fishing activities, which in turn highlights the need for better regional litter management measures.

Equilibrium partitioning approach for metal toxicity assessment in tropical estuarine sediment of Bandon Bay, Thailand.

An equilibrium partitioning approach (EqPA) was employed to evaluate the metal toxicity and define sediment quality guidelines (SQGs) for arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), nickel (Ni), lead (Pb), zinc (Zn), and mercury (Hg) in the cockle cultivated areas located in Bandon Bay, Thailand. An assessment of metal toxicity using the [∑SEM]-[AVS] and [∑SEM]-[AVS]/foc models indicated no adverse effect on benthic organisms. The normalized total metal concentrations in this area were below the established SQG values for As, Cd, Cu, Ni, Pb, Zn, and Hg, namely respectively 21.3, 0.8, 84.6, 36.0, 34.6, 440.9 mg/kg dry weight, and 49.3 µg/kg dry weight on sand and calcium carbonate free with 1 % total organic carbon basis, suggesting low metal toxicity. This study provides locality adapted SQG values for supporting sediment quality management specifically in Bandon Bay, potentially serving as a model for other coastal areas.

The comparative plastisphere microbial community profile at Kung Wiman beach unveils potential plastic-specific degrading microorganisms.

Background: Plastic waste is a global environmental issue that impacts the well-being of humans, animals, plants, and microorganisms. Microplastic contamination has been previously reported at Kung Wiman Beach, located in Chanthaburi province along with the Eastern Gulf of Thailand. Our research aimed to study the microbial population of the sand and plastisphere and isolate microorganisms with potential plastic degradation activity. Methods: Plastic and sand samples were collected from Kung Wiman Beach for microbial isolation on agar plates. The plastic samples were identified by Fourier-transform infrared spectroscopy. Plastic degradation properties were evaluated by observing the halo zone on mineral salts medium (MSM) supplemented with emulsified plastics, including polystyrene (PS), polylactic acid (PLA), polyvinyl chloride (PVC), and bis (2-hydroxyethyl) terephthalate (BHET). Bacteria and fungi were identified by analyzing nucleotide sequence analysis of the 16S rRNA and internal transcribed spacer (ITS) regions, respectively. 16S and ITS microbiomes analysis was conducted on the total DNA extracted from each sample to assess the microbial communities. Results: Of 16 plastic samples, five were identified as polypropylene (PP), four as polystyrene (PS), four as polyethylene terephthalate (PET), two as high-density polyethylene (HDPE), and one sample remained unidentified. Only 27 bacterial and 38 fungal isolates were found to have the ability to degrade PLA or BHET on MSM agar. However, none showed degradation capabilities for PS or PVC on MSM agar. Notably, Planococcus sp. PP5 showed the highest hydrolysis capacity of 1.64 ± 0.12. The 16S rRNA analysis revealed 13 bacterial genera, with seven showing plastic degradation abilities: Salipiger, Planococcus, Psychrobacter, Shewanella, Jonesia, Bacillus, and Kocuria. This study reports, for the first time of the BHET-degrading properties of the genera Planococcus and Jonesia. Additionally, The ITS analysis identified nine fungal genera, five of which demonstrated plastic degradation abilities: Aspergillus, Penicillium, Peacilomyces, Absidia, and Cochliobolus. Microbial community composition analysis and linear discriminant analysis effect size revealed certain dominant microbial groups in the plastic and sand samples that were absent under culture-dependent conditions. Furthermore, 16S and ITS amplicon microbiome analysis revealed microbial groups were significantly different in the plastic and sand samples collected. Conclusions: We reported on the microbial communities found on the plastisphere at Kung Wiman Beach and isolated and identified microbes with the capacity to degrade PLA and BHET.

Heavy metal bioaccumulation and risk assessment in fishery resources from the Gulf of Thailand.

The muscle tissues of 19 fish species, two crab species, and one shrimp species collected from the Gulf of Thailand (GoT) were analyzed to determine the levels of heavy metals, including Cu, Zn, Fe, Mn, Ni, Pb, Cd, and Hg. The results revealed that the mean concentrations of the heavy metals, in descending order, were Zn > Cu > Fe > Cd > Hg > Mn > Pb > Ni. Among the examined metals, zinc was found to be the most prevalent in fish tissues. Based on the risk assessment indices, the estimated average daily doses (ADD) of the heavy metals were found to be below the provisional tolerable daily intake (PTDI) recommended by the joint Committee of the Food and Agriculture Organization (FAO) and the World Health Organization (WHO) on food contaminants. The results of the target cancer risk analysis revealed no related cancer risk from the consumption of the fishes considered for the study. However, the target hazard quotient (THQ) values exceeded the threshold of 1 (THQ > 1) specifically for mercury in Gymnothorax spp. and Terapon spp. Furthermore, the calculated hazard index (HI) values for fish muscles were all below 1, indicating that there is no significant health risk for humans at the current consumption rates, except in Terapon species for both normal and habitual consumers. Notably, habitual consumers of Gymnothorax species showed the highest HI value (>1), suggesting potential long-term effects on human health when consuming larger quantities of these fishes.

Riverine and submarine groundwater nutrients fuel high primary production in a tropical bay.

River discharge has long been recognized as a major source of nutrients supporting high primary production (PP) in Bandon Bay, while submarine groundwater discharge (SGD) and atmospheric deposition have largely been overlooked. In this study, we evaluated contributions of nutrients via river, SGD and atmospheric deposition, and their roles on PP in the bay. Contribution of nutrients from the three sources during different time of the year was estimated. Nutrients supply from Tapi-Phumduang River accounted for two-fold the amount from SGD while very little supply was from atmospheric deposition. Significant seasonal difference in silicate and dissolved inorganic nitrogen were observed in river water. Dissolved phosphorous in river water was mainly (80 % to 90 %) of DOP in both seasons. For the bay water, DIP in wet season was two-fold higher than in dry season while dissolved organic phosphorus (DOP) was only one half of those measured in dry season. In SGD, dissolved nitrogen was mostly inorganic (with 99 % as NH4+), while dissolved phosphorous was predominantly (DOP). In general, Tapi River is the most important source of nitrogen (NO3-, NO2-, and DON), contributing >70 % of all considered sources, especially in wet season, while SGD is a major source for DSi, NH4+ and phosphorus, contributing 50 % to 90 % of all considered sources. To this end, Tapi River and SGD deliver a large quantity of nutrients and support high PP in the bay (337 to 553 mg-C m-2 day-1).

Preindustrial levels and temporal enrichment trends of mercury in sediment cores from the Gulf of Thailand.

Four sediment cores in the middle of Gulf of Thailand (GOT) and one core close to Bang Pakong River mouth were examined for total mercury (T-Hg) using direct thermal decomposition coupled with the atomic absorption spectrometry (DTD-AAS) method and acid digestion (acid-CVAAS) method, and sediment chronologies using 210Pb dating. T-Hg in the river mouth core ranged 44.49-52.76 µg/kg and higher than the cores from the middle of GOT (18.26-36.68 µg/kg). The age span obtained from the cores dated back to the 1940s with the sediment accumulation rates of 0.15-0.76 cm/year. The preindustrial levels of T-Hg showed an initial slow increase followed by a rapid elevation since the 1960s which marked the start of the industrialized period in the country. To this end, we posit that T-Hg in the GOT sediment can be attributed to not only land-based sources but also offshore activities including petroleum exploration and frequent accidental oil spills.

Abundance, composition, and fate of microplastics in water, sediment, and shellfish in the Tapi-Phumduang River system and Bandon Bay, Thailand.

Microplastic contamination in the environment is a global problem, as evidenced by the increasing amount of research worldwide. To our knowledge, this study is the first to investigate the microplastic distribution in Bandon Bay, one of the most important maricultural areas of Thailand. Water and sediment samples from the Tapi-Phumduang River system (n = 10) and Bandon Bay (n = 5) were collected. Water sampling at the river mouth was carried out during a complete tidal cycle to estimate the microplastic flux to the bay during the wet season. Moreover, two commercial bivalve species grown in the bay, the green mussel (Perna viridis) and lyrate Asiatic hard clam (Meretrix lyrata), were analyzed. More items of microplastics were found in the river system than in the bay. During the tide cycle, one-third of the microplastics entering the bay were washed back upstream during high tide. This backflow consisted mainly of larger microplastics. The average daily load of microplastics to the bay was 22.4 × 109 items day-1. The load during low tide was approximately 4-5 times higher than that during high tide. The overall accumulation of microplastics in the bottom sediments of the river and in the bay was similar (p < 0.05). Green mussels showed significantly higher contamination with microplastics than clams. Notably, the small-sized shellfish contained more particles (items/g) than the large ones (p < 0.05). Fibers were detected in virtually all samples: water (98%), sediment (94%), mussels (100%), and clams (95%). Among these, microfibers (<1 mm) were detected in water (71%), sediment (63%), green mussels (63%), and clams (52%). Blue and white particles were the two most frequently observed colors, while the most dominant polymers were rayon, followed by polypropylene (PP) or polyethylene (PE), polyethylene terephthalate (PET), and nylon. To this end, we posit that river discharge was a significant source of microplastics in Bandon Bay, with minor additional contributions from fishing and mariculture activities within the bay. Ultimately, these microplastics may end up in the sediments and living organisms.

Evidence for fungal and chemodenitrification based N2O flux from nitrogen impacted coastal sediments.

Although increasing atmospheric nitrous oxide (N2O) has been linked to nitrogen loading, predicting emissions remains difficult, in part due to challenges in disentangling diverse N2O production pathways. As coastal ecosystems are especially impacted by elevated nitrogen, we investigated controls on N2O production mechanisms in intertidal sediments using novel isotopic approaches and microsensors in flow-through incubations. Here we show that during incubations with elevated nitrate, increased N2O fluxes are not mediated by direct bacterial activity, but instead are largely catalysed by fungal denitrification and/or abiotic reactions (e.g., chemodenitrification). Results of these incubations shed new light on nitrogen cycling complexity and possible factors underlying variability of N2O fluxes, driven in part by fungal respiration and/or iron redox cycling. As both processes exhibit N2O yields typically far greater than direct bacterial production, these results emphasize their possibly substantial, yet widely overlooked, role in N2O fluxes, especially in redox-dynamic sediments of coastal ecosystems.