RESUMO
With the increasing use of small animals for human disease studies, small-animal whole-body molecular imaging plays an important role in biomedical research. Currently, none of the existing imaging modalities can provide both anatomical and glucose molecular information, leading to higher costs of building dual-modality systems. Even with image co-registration, the spatial resolution of the molecular imaging modality is not improved. Utilizing a ring-shaped confocal photoacoustic computed tomography system, we demonstrate, for the first time, that both anatomy and glucose uptake can be imaged in a single modality. Anatomy was imaged with the endogenous hemoglobin contrast, and glucose metabolism was imaged with a near-infrared dye-labeled 2-deoxyglucose.
Assuntos
Glucose/metabolismo , Neoplasias Experimentais/metabolismo , Neoplasias Experimentais/patologia , Técnicas Fotoacústicas/métodos , Tomografia Computadorizada por Raios X/métodos , Imagem Corporal Total/métodos , Animais , Linhagem Celular Tumoral , Camundongos , Camundongos Nus , Imagem Molecular , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.