RESUMO
The main purpose of this research was to clarify the influence of the addition of iron (Fe) alone (0-100 mg/L) or 50 mg/L of Fe with 2 mg/L each of cobalt (Co), copper (Cu) and nickel (Ni) on the methanogenic activity of a mesophilic two-stage UASB system treating ethanol wastewater at a fixed chemical oxygen demand (COD) loading rate of 16 kg/m3/day under a continuous mode of operation and steady state condition. The addition of Fe provided the dual benefits of a reduction in both the dissolved sulfide and the hydrogen sulfide (H2S) content in produced gas, resulting in marginally improved hydrogen (H2) and methane (CH4) productivities. When the Fe dosage was increased beyond the optimum value of 50 mg/L, the process performance drastically declined, as a consequence of the high total volatile fatty acid (VFA) concentrations that inhibited both the acidogens and methanogens predominantly present in the 1st and 2nd reactors, respectively. The chemical precipitation of iron sulfide was responsible for the reduction of produced H2S in both the aqueous and gaseous phases as well as the minimization of added amounts of all other micronutrients to fulfil the sufficiency of all micronutrients for anaerobic digestion (AD). The addition of 2 mg/L each of Co, Cu and Ni together with 50 mg/L Fe resulted in the greatest enhancement in process performance, as indicated by the improved CH4 yield (mL/g COD applied) to about 42.3%, compared to that without micronutrient supplement.
Assuntos
Reatores Biológicos , Oligoelementos , Esgotos/química , Anaerobiose , Micronutrientes , Metano/química , Enxofre , GasesRESUMO
Residual oil in palm oil mill effluent (POME) poses difficulties in its treatment chain. Non-ionic surfactants containing different hydrophobic tail structures and their optimal concentrations were evaluated for effectiveness in biohydrogen production. By adding the surfactants at their critical micelle concentration in synthetic oily wastewater, the maximal H2 yield was increased by 2.2 and 3.5 times for Triton X-100 and Tergitol 15-S-9, respectively, compared to the control. Using real POME, the supplemental Tergitol 15-S-9 resulted in a 56.4 % improvement in H2 production. For continuous digestion studies, pure POME and Tergitol 15-S-9 supplemented POME (sPOME) were fed to thermophilic anaerobic sequencing batch reactors (ASBRs) under hydraulic retention time (HRT) of 32-12.5 days. Optimally at HRT 19 days, H2 content in the biogas from sPOME-fed ASBR was noticeably higher, which gave a superior yield of 203.4 mLH2/gCODremoved (+15 %).
Assuntos
Reatores Biológicos , Águas Residuárias , Anaerobiose , Fermentação , Óleo de Palmeira , Poloxaleno , TensoativosRESUMO
A new UASB (upflow anaerobic sludge blanket) system with three-stage concept and granular sludge formation was developed to provide a very high process activity, in terms of a high energy yield and a large optimum COD loading rate, from ethanol wastewater at 37 °C and a controlled pH of 5.5 in the first bioreactor with recirculation of the final overflow from the third to the first two bioreactors to lower the amount of sodium hydroxide used for pH control in the first bioreactor and to increase the total alkalinity of the whole system. The proper volumetric ratio of the three-stage UASB with flocculant sludge provided the dominance of the three sequencial steps of acidogenesis, acetognesis and methanogenesis to occur in the respective bioreactors, causing a higher process performance and giving the optimum COD loading rate of 15 kg/m3d. The presence of granular sludge in all three bioreactors, resulted from the slow increment in the COD loading rate over a long operation period with high levels of microbial concentrations in all three bioreactors under the continuous feed operation and steady sate condition. This further increased the optimum COD loading rate from 15 to 25 kg/m3d (calculated from the total liquid volume) with an extremely high energy yield of 11,740 kJ/kg COD applied.
Assuntos
Esgotos , Eliminação de Resíduos Líquidos , Anaerobiose , Reatores Biológicos , Metano , Águas ResiduáriasRESUMO
In this research, the separate production of hydrogen (H2) and methane (CH4) from cassava wastewater with added cassava residue was investigated using a two-stage upflow anaerobic sludge blanket (UASB) system under thermophilic temperature (55 °C) in a continuous mode of operation and steady state condition. The two-stage UASB system was operated under an optimum chemical oxygen demand (COD) loading rate of 10.29 kg/m3d (based on the total volume of both bioreactors) of the cassava wastewater with different concentrations of added cassava residue. The recycle ratio of the effluent from the second bioreactor to the feed flow rate was fixed at 1:1 (v/v). In addition, the solution pH in the first bioreactor was controlled at 5.5, while that in the second bioreactor was not controlled. Under the optimum cassava residue concentration of 1200 mg/L, the produced gas from the first bioreactor contained 42.3% H2, 55% carbon dioxide (CO2) and 2.70% CH4, while that from the second bioreactor contained 70.5% CH4, 28% CO2 and 1.5% H2. Apart from a high H2 and CH4 production performance (45.2 and 150% improvement, respectively, as compared to the system without added cassava residue) under the optimum cassava residue concentration (1200 mg/L) and the controlled COD loading rate (10.29 kg/m3d) of the cassava wastewater, the degradation performance of cellulose and hemicellulose were 41% and 22%, respectively, for the first bioreactor and 23% and 11%, respectively, for the second bioreactor. The digestibility of the cassava residue at thermophilic operation was higher than that at mesophilic temperature.
Assuntos
Reatores Biológicos , Celulose/metabolismo , Hidrogênio/metabolismo , Manihot , Metano/metabolismo , Águas Residuárias , Temperatura , Eliminação de Resíduos Líquidos/métodosRESUMO
Nonionic surfactants have been utilized to improve the enzymatic hydrolysis of lignocellulosic materials. However, the role of surfactant adsorption affecting enzymatic hydrolysis has not been elaborated well. In this work, nonionic surfactants differing in their molecular structures, namely the polyoxyethylene sorbitan monooleate (Tween 80), the secondary alcohol ethoxylate (Tergitol 15-S-9), and the branched alcohol ethoxylate (Tergitol TMN-6), were studied for their effects on the enzymatic hydrolysis of palm fruit bunch (PFB). The PFB was pretreated with a 10% w/v sodium hydroxide solution and then hydrolyzed using the cellulase enzyme from Trichoderma reesei (ATCC 26921) at 50 °C and pH 5. The optimal conditions providing similar yields of reducing sugar required Tween 80 and Tergitol TMN-6 at 0.25% w/v, while Tergitol 15-S-9 was required at 0.1% w/v. All the surfactants improved the enzymatic conversion efficiency and reduced unproductive binding of the enzyme to lignin. In addition, the adsorption isotherm of cellulase was fit well by the Freundlich isotherm, while adsorption of the three nonionic surfactants agreed well with the Langmuir isotherm. Adsorption capacities of the three nonionic surfactants were consistent with their enhancement efficiencies in hydrolysis. The critical micelle concentration was observed as a key property of nonionic surfactant for adsorption capacity.
Assuntos
Arecaceae/química , Celulase/química , Frutas/química , Proteínas Fúngicas/química , Tensoativos/química , Trichoderma/enzimologia , HidróliseRESUMO
Laundry detergency of solid non-particulate soil on polyester and cotton was investigated using a microemulsion-based formulation, consisting of an anionic extended surfactant (C12,13-4PO-SO4Na) and sodium mono-and di-methyl naphthalene sulfonate (SMDNS) as the hydrophilic linker, to provide a Winsor Type III microemulsion with an ultralow interfacial tension (IFT). In this work, methyl palmitate (palmitic acid methyl ester) having a melting point around 30°C, was used as a model solid non-particulate (waxy) soil. A total surfactant concentration of 0.35 wt% of the selected formulation (4:0.65 weight ratio of C12,13-4PO-SO4Na:SMDNS) with 5.3 wt% NaCl was able to form a middle phase microemulsion at a high temperature (40°C),which provided the highest oil removal level with the lowest oil redeposition and the lowest IFT, and was much higher than that with a commercial detergent or de-ionized water. Most of the detached oil, whether in liquid or solid state, was in an unsolubilized form. Hence, the dispersion stability of the detached oil droplets or solidified oil particles that resulted from the surfactant adsorption played an important role in the oil redeposition. For an oily detergency, the lower the system IFT, the higher the oil removal whereas for a waxy (non-particulate) soil detergency, the lower the contact angle, the higher the solidified oil removal. For a liquefied oil, the detergency mechanism was roll up and emulsification with dispersion stability, while that for the waxy soil (solid oil) was the detachment by wettability with dispersion stability.
Assuntos
Detergentes/química , Lavanderia , Palmitatos , Fibra de Algodão , Emulsões , Interações Hidrofóbicas e Hidrofílicas , Material Particulado , Poliésteres , Tensão Superficial , Tensoativos , Temperatura , TêxteisRESUMO
Background: Endoglucanase plays a major role in initiating cellulose hydrolysis. Various wild-type strains were searched to produce this enzyme, but mostly low extracellular enzyme activities were obtained. To improve extracellular enzyme production for potential industrial applications, the endoglucanase gene of Bacillus subtilis M015, isolated from Thai higher termite, was expressed in a periplasmic-leaky Escherichia coli. Then, the crude recombinant endoglucanase (EglS) along with a commercial cellulase (Cel) was used for hydrolyzing celluloses and microbial hydrolysis using whole bacterial cells. Results: E. coli Glu5 expressing endoglucanase at high levels was successfully constructed. It produced EglS (55 kDa) with extracellular activity of 18.56 U/mg total protein at optimal hydrolytic conditions (pH 4.8 and 50°C). EglS was highly stable (over 80% activity retained) at 4050°C after 100 h. The addition of EglS significantly improved the initial sugar production rates of Cel on the hydrolysis of carboxymethyl cellulose (CMC), microcrystalline cellulose, and corncob about 5.2-, 1.7-, and 4.0-folds, respectively, compared to those with Cel alone. E. coli Glu5 could secrete EglS with high activity in the presence of glucose (1% w/v) and Tween 80 (5% w/v) with low glucose consumption. Microbial hydrolysis of CMC using E. coli Glu5 yielded 26 mg reducing sugar/g CMC at pH 7.0 and 37°C after 48 h. Conclusions: The recombinant endoglucanase activity improved by 17 times compared with that of the native strain and could greatly enhance the enzymatic hydrolysis of all studied celluloses when combined with a commercial cellulase.
Assuntos
Bacillus subtilis/enzimologia , Celulase/metabolismo , Isópteros/microbiologia , Tailândia , Proteínas Recombinantes/metabolismo , Celulase/genética , Celulose , Amplificação de Genes , Agricultura , Escherichia coli/metabolismo , HidróliseRESUMO
The objective of this study was to investigate the separate hydrogen and methane productions from cassava wastewater by using a two-stage upflow anaerobic sludge blanket (UASB) system under thermophilic operation. Recycle ratio of the effluent from methane bioreactor-to-feed flow rate was fixed at 1:1 and pH of hydrogen UASB unit was maintained at 5.5. At optimum COD loading rate of 90 kg/m3 d based on the feed COD load and hydrogen UASB volume, the produced gas from the hydrogen UASB unit mainly contained H2 and CO2 which provided the maximum hydrogen yield (54.22 ml H2/g COD applied) and specific hydrogen production rate (197.17 ml/g MLVSSd). At the same optimum COD loading rate, the produced gas from the methane UASB unit mainly contained CH4 and CO2 without H2 which were also consistent with the maximum methane yield (164.87 ml CH4/g COD applied) and specific methane production rate (356.31 ml CH4/g MLVSSd). The recycling operation minimized the use of NaOH for pH control in hydrogen UASB unit.
Assuntos
Anaerobiose , Hidrogênio/metabolismo , Manihot , Metano/metabolismo , Esgotos , Águas ResiduáriasRESUMO
The effect of cholesterol on the vesicle formation of a rhamnolipid biosurfactant extracted from the liquid culture of Pseudomonas aeruginosa SP4 was investigated. The rhamnolipid vesicles were prepared in a phosphate-buffer saline (PBS) solution (pH 7.4) at a biosurfactant concentration of 2.6mM, or 6.5 times the critical micelle concentration (CMC), with various amounts of cholesterol. The biosurfactant solution was characterized using turbidity, zeta potential, and dynamic light scattering (DLS) measurements. The morphology of the rhamnolipid vesicles formed at different cholesterol concentrations was examined with the use of transmission electron microscopy (TEM). The results showed that the rhamnolipid biosurfactant formed spherical vesicles both in the absence and presence of cholesterol, but the incorporation of the cholesterol into the bilayer membrane reduced the vesicle size. Sudan III, a water-insoluble dye, was used as a model hydrophobic compound in the encapsulation experiment. The encapsulation efficiency (E%) of the rhamnolipid vesicles was affected by the cholesterol concentration and the initial Sudan III concentration. The maximum E% of nearly 90% was achieved at the cholesterol concentration of 100µM and the initial Sudan III concentration of 8.8µM.
Assuntos
Fatores Biológicos/metabolismo , Colesterol/química , Glicolipídeos/metabolismo , Pseudomonas aeruginosa/metabolismo , Tensoativos/metabolismo , Compostos Azo/química , Fatores Biológicos/química , Glicolipídeos/química , Concentração de Íons de Hidrogênio , Micelas , Tensoativos/químicaRESUMO
The three highest hydrolysis-capacity-value isolates of Bacillus subtilis (A 002, M 015, and F 018) obtained from Thai higher termites, Microcerotermes sp., under different isolation conditions (aerobic, anaerobic, and anaerobic/aerobic) were tested for cellulase activities--FPase, endoglucanase, and ß-glucosidase--at 37 °C and pH 7.2 for 24 h. Their tolerance to an ionic liquid, 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), was also investigated. The results showed that the isolate M 015 provided the highest endoglucanase activity whereas the highest FPase and ß-glucosidase activities were observed for the isolate F 018. The isolate F 018 also showed the highest tolerance to [BMIM]Cl in the range of 0.1-1.0 vol.%. In contrast, the isolate A 002 exhibited growth retardation in the presence of 0.5-1.0 vol.% [BMIM]Cl.
Assuntos
Adaptação Fisiológica/efeitos dos fármacos , Bactérias/efeitos dos fármacos , Bactérias/enzimologia , Celulase/biossíntese , Imidazóis/toxicidade , Líquidos Iônicos/toxicidade , Isópteros/microbiologia , Animais , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Proteínas de Bactérias/biossíntese , Temperatura , TailândiaRESUMO
In this research, a continuous multistage ion foam fractionation column with bubble-cap trays was employed to remove cadmium ions from simulated wastewater having cadmium ions at a low level (10 mg/L). In this study, sodium dodecyl sulfate (SDS) was used to generate the foam. An increase in feed SDS/Cd molar ratio enhanced the removal of Cd. However, the SDS concentration above a certain level resulted in wetter foams, leading to having a high volume of generated foam that lowered both the enrichment ratio and separation factor of the Cd. The SDS recovery tended to increase with increasing feed SDS/Cd molar ratio. The molar ratio of SDS/Cd in foamate was found to be close to the theoretical adsorption molar ratio of 2/1 on the air-water interface of foam when the system was operated at a feed SDS/Cd molar ratio in the range of 2/1-7/1. Ion foam fractionation has been demonstrated in this study to be a promising technique for high heavy metal removal (more than 99%) for a feed having a low heavy metal concentration in the ppm (mg/L) level.
Assuntos
Cádmio/isolamento & purificação , Dodecilsulfato de Sódio/químicaRESUMO
Pseudomonasaeruginosa SP4 was cultivated to produce a rhamnolipid biosurfactant from a nutrient broth with palm oil. The foam fractionation technique in batch mode was used for the recovery of the excreted biosurfactant from the free-cell culture medium. The effects of air flow rate, initial foam height, the pore size of the sintered glass disk, initial liquid volume, and operation time on the process performance were studied. The results showed that the operating conditions were optimized at an air flow rate of 30 ml/min, an initial foam height of 60 cm, a pore size of the sintered glass disk in the range of 160-250 microm (No. 0), an initial liquid volume of 25 ml, and an operation time of 4 h, providing a biosurfactant recovery of 97% and an enrichment ratio of 4. The HPLC results also indicated that the rhamnolipid was concentrated by using the foam fractionation technique.
Assuntos
Fracionamento Químico/métodos , Glicolipídeos/isolamento & purificação , Glicolipídeos/metabolismo , Pseudomonas aeruginosa/classificação , Pseudomonas aeruginosa/metabolismo , Tensoativos/isolamento & purificação , Tensoativos/metabolismo , Gases/química , Especificidade da EspécieRESUMO
A biosurfactant produced by Pseudomonas aeruginosa strain SP4 was previously reported as a mixture of 11 types of rhamnolipid compounds. Among them, the major component in the biosurfactant was characterized as l-rhamnosyl-3-hydroxydecanoyl-3-hydroxydecanoate, or monorhamnolipid (Rha-C(10)-C(10)). In this present study, solution properties of the biosurfactant were investigated in a phosphate-buffer saline (PBS) solution (pH 7.4) by using surface tension, turbidity, electrical conductivity, and dynamic light scattering (DLS) measurements. It was found that spherical biosurfactant vesicles of various sizes (ranging from 50 to larger than 250 nm) were spontaneously formed at a biosurfactant concentration greater than its critical micelle concentration (CMC), which was 200mg/l. The encapsulation efficiency (E%) of the biosurfactant vesicles was preliminarily studied by using Sudan III, a water-insoluble dye, as a model hydrophobic substance. The obtained results showed that the vesicle formed in the PBS solution at a biosurfactant concentration of 1280 mg/l could entrap about 10% of the initial hydrophobic dye concentration. The effects of salt and alcohol on the vesicle formation of the biosurfactant and its encapsulation efficiency were also observed by using sodium chloride (NaCl) and ethanol (C(2)H(5)OH), respectively. In the presence of either NaCl or C(2)H(5)OH, the vesicle size was reduced from larger than 250 nm to 50-250 nm. The encapsulation efficiency of the biosurfactant vesicle was slightly influenced by the addition of NaCl, but was significantly increased, up to nearly 30%, in the presence of C(2)H(5)OH.
Assuntos
Glicolipídeos/química , Pseudomonas aeruginosa/metabolismo , Tensoativos/química , Compostos Azo/química , Condutividade Elétrica , Etanol/farmacologia , Luz , Nefelometria e Turbidimetria , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/ultraestrutura , Espalhamento de Radiação , Cloreto de Sódio/farmacologia , Soluções , Propriedades de Superfície/efeitos dos fármacos , Tensão Superficial/efeitos dos fármacosRESUMO
Mannosylerythritol lipids (MELs) are glycolipid biosurfactants (BS) abundantly produced from renewable resources by yeast strains of the genus Pseudozyma. In this study, the ternary phase behaviors of two types of MELs, i.e. MEL-A and MEL-B, mixed with water and oil were investigated at 25 degrees C based on polarized optical microscopy and small-angle X-ray scattering (SAXS). When n-decane was used as an oil phase, diacetylated MEL-A formed single-phase water-in-oil (W/O) microemulsion in a remarkably large region. MEL-A, with a negative spontaneous curvature, also formed sponge (L(3)), reverse bicontinuous cubic (V(2)), and lamellar (L(alpha)) phases. Meanwhile, monoacetylated MEL-B, with the opposite configuration of the erythritol moiety, gave single-phase bicontinuous microemulsion and showed a triangular phase diagram dominated by the L(alpha) phase, suggesting that MEL-B has an almost zero spontaneous curvature. Moreover, we succeeded in preparation of oil-in-liquid crystal (O/LC) emulsion in the biphasic L(alpha)+O region of the MEL-B/water/n-decane system. The obtained gel-like emulsion was stable for at least 1 month. These results clearly demonstrated that the difference in the number of acetyl group on the headgroup and/or the chirality of the erythritol moiety drastically changed the phase behavior of MELs. Accordingly, these MELs would be quite distinctive from conventional BS hitherto reported, and would have great potential for the preparation of microemulsion and LC-based emulsion.
Assuntos
Glicolipídeos/química , Óleos/química , Transição de Fase , Água/química , Alcanos/química , Cristais Líquidos/química , TemperaturaRESUMO
In this present study, sequencing batch reactors (SBRs) were used for biosurfactant production from Pseudomonasaeruginosa SP4, which was isolated from petroleum-contaminated soil in Thailand. Two identical lab-scale aerobic SBR units were operated at a constant temperature of 37 degrees C, and a mineral medium (MM) with palm oil was used as the culture medium. The effects of oil loading rate (OLR) and cycle time on the biosurfactant production were studied. The results indicated that the optimum conditions for the biosurfactant production were at an OLR of 2 kg/m(3)days and a cycle time of 2 days/cycle, which provided a surface tension reduction of 59%, a chemical oxygen demand (COD) removal of 90%, and an oil removal of 97%. Under the optimum conditions, it was found that the biosurfactant production was maximized at an aeration time of 40 h. These preliminary results suggest that the SBR can potentially be adapted for biosurfactant production, and perhaps further developed, potentially for large-scale biosurfactant production.
Assuntos
Reatores Biológicos/microbiologia , Óleos de Plantas/metabolismo , Pseudomonas aeruginosa/metabolismo , Tensoativos/metabolismo , Óleo de Palmeira , Fatores de TempoRESUMO
Mannosylerythritol lipids (MELs) are one of the most promising glycolipid biosurfactants produced by yeast strains of the genus Pseudozyma. In this study, the aqueous-phase behavior of a new monoacetyl MEL derivative, 1-O-beta-(2',3'-di-O-alka(e)noyl-6'-O-acetyl-d-mannopyranosyl)-d-erythritol (MEL-B), was investigated using polarized optical microscopy, small-angle X-ray scattering (SAXS), confocal laser scanning microscopy (CLSM), and differential scanning calorimetry (DSC). The present MEL-B was found to self-assemble into a lamellar (L(alpha)) phase over remarkably wide concentration and temperature ranges. According to SAXS measurement, the interlayer spacing (d) was estimated to be almost constant (about 4.7 nm) at the low MEL-B concentration (
Assuntos
Glicolipídeos/química , Tensoativos/química , Lipossomas Unilamelares/síntese química , Varredura Diferencial de Calorimetria , Espalhamento a Baixo Ângulo , Estereoisomerismo , Ustilaginales/química , Ustilaginales/metabolismo , Difração de Raios XRESUMO
Ozone is a strong oxidant, which can oxidize both biodegradable and non-biodegradable organics. The main objective of this study was to use iron oxide as a heterogeneous catalyst to enhance the ozone oxidation process. The wastewater used in this study was distillery wastewater, which was diluted 20 times before use. The diluted distillery wastewater was fed continuously in a downflow direction in an ozonation column. The iron oxide catalyst was coated on 10.3mm diameter alumina balls (5.5 m2/g specific surface area) by using Fe(NO3)3 as a precursor. The prepared catalyst was in the form of ferric oxide, and its loading was 0.07%. From the experimental results of both with and without the iron oxide catalyst, an increase in hydraulic retention time resulted in an increase in the treatment efficiencies of both chemical oxygen demand (COD) and color reduction, since the residence time of ozone increased. When the ozone mass flow rate increased, both COD and color reduction increased, resulting from an increase in the hydroxyl radical available in the system. The ozonation system with the iron oxide catalyst gave the highest efficiency in both COD and color removals because the hydroxyl free radical generated from the catalyst is more reactive than the ozone molecule itself.
Assuntos
Cor , Compostos Férricos/química , Resíduos Industriais , Ozônio/química , Poluentes Químicos da Água/isolamento & purificação , Catálise , Espectrofotometria Ultravioleta , Difração de Raios XRESUMO
Pseudomonas aeruginosa strain SP4, isolated from petroleum-contaminated soil in Thailand, was used to produce a biosurfactant from a nutrient broth with palm oil as the carbon source. The key components of the crude biosurfactant were fractionated by using HPLC-ELSD technique. With the use of ATR-FTIR spectroscopy, in combination with (1)H NMR and MS analyses, chemical structures of the fractionated components of the crude biosurfactant were identified as rhamnolipid species. When compared to synthetic surfactants, including Pluronic F-68, which is a triblock nonionic surfactant containing poly(ethylene oxide) and poly(propylene oxide), and sodium dodecyl sulfate, the crude biosurfactant showed comparable physicochemical properties, in terms of the surface activities. The crude biosurfactant reduced the surface tension of pure water to 29.0 mN/m with a critical micelle concentration of approximately 200 mg/l, and it exhibited good thermal and pH stability. The crude biosurfactant also formed stable water-in-oil microemulsions with crude oil and various types of vegetable oils, but not with short-chain hydrocarbons.
Assuntos
Biotecnologia/métodos , Pseudomonas aeruginosa/metabolismo , Poluentes do Solo/química , Tensoativos/química , Biodegradação Ambiental , Poluição Ambiental , Hidrocarbonetos/química , Concentração de Íons de Hidrogênio , Petróleo , Poloxâmero/química , Polietilenoglicóis/química , Polímeros/química , Propilenoglicóis/química , Dodecilsulfato de Sódio/química , Espectroscopia de Infravermelho com Transformada de Fourier , Tensão SuperficialRESUMO
Mannosylerythritol lipid-A (MEL-A) is a glycolipid biosurfactant abundantly produced from soybean oil by microorganisms at a yield of up to 100 g/L. In this study, the formation of water-in-oil (W/O) microemulsion based on the single component of MEL-A was confirmed using dynamic light scattering (DLS) and freeze fracture electron microscopy (FF-EM). DLS and FF-EM measurements revealed that the diameter of the microemulsion increases with an increase in water-to-surfactant mole ratio (W(0)) ranging from 20 to 60 nm, and the maximum W(0) value was found to be 20, which is as high as that of soybean lecithin. Glycolipid biosurfactant has a great potential for the formation of W/O microemulsion without using any cosurfactants.
Assuntos
Glicolipídeos/química , Lipídeo A/química , Óleo de Soja/química , Tensoativos/química , Microscopia Crioeletrônica , Emulsões , Água/químicaRESUMO
A simple route of combined sol-gel process with surfactant-assisted templating technique was successfully employed for the first time to synthesize nanocrystalline mesoporous Dy(2)O(3) with narrow monomodal pore size distribution under mild conditions. The nanocrystalline Dy(2)O(3) with monomodal mesoporous characteristic was ultimately achieved by controlling the hydrolysis and condensation steps of dysprosium n-butoxide modified with acetylacetone in the presence of laurylamine hydrochloride surfactant aqueous solution. The synthesized material was methodically characterized by thermogravimetry and differential thermal analysis (TG-DTA), X-ray diffraction (XRD), scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM), selected-area electron diffraction (SAED), N(2) adsorption-desorption, Brunauer-Emmett-Teller (BET) surface area analysis, and Barrett-Joyner-Halenda (BJH) pore size distribution analysis. The particle size of the synthesized Dy(2)O(3) in nanosized range obtained from the SEM and HRTEM micrographs was in good accordance with the crystallite size estimated from the XRD result. The N(2) adsorption-desorption result exhibited hysteresis pattern with single loop, indicating the existence of monomodal mesopore. The extremely narrow pore size distribution with mean pore diameter in the mesopore region of the synthesized Dy(2)O(3) was also confirmed by the BJH result.