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The clinical treatment of chronic diabetic wounds is a long-standing thorny issue. Strategies targeting the diabetic micro-environment have been developed to promote wound healing. However, it remains challenging to reverse the adverse conditions and re-activate tissue regeneration and angiogenesis. In this work, we develop injectable hydrogels that are responsive to acidic conditions, reactive oxygen species (ROS), and high glucose levels in a diabetic wound micro-environment to sustainably deliver tannic acid (TA) to augment antibacterial, anti-inflammatory, and anti-oxidative activities. This triple-responsive mechanism is designed by introducing dynamic acylhydrazone and phenylboronic ester bonds to crosslink modified hyaluronic acid (HA) chains. At a diabetic wound, the acylhydrazone bonds may be hydrolyzed at low pH. Meanwhile, glucose may compete with TA, and ROS may oxidize the C-B bond to release TA. Thus, sustained release of TA is triggered by the diabetic micro-environment. The released TA effectively scavenges ROS and kills bacteria. In vivo experiments on diabetic mice demonstrate that the hydrogel dressing highly promotes angiogenesis and extracellular matrix (ECM) deposition, leading to eventual full healing of diabetic skin wounds. This micro-environment-triggered triple-responsive drug release provides a promising method for chronic diabetic wound healing.
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Antibacterianos , Diabetes Mellitus Experimental , Ácido Hialurônico , Hidrogéis , Cicatrização , Ácido Hialurônico/química , Ácido Hialurônico/farmacologia , Cicatrização/efeitos dos fármacos , Antibacterianos/farmacologia , Antibacterianos/química , Animais , Hidrogéis/química , Hidrogéis/farmacologia , Camundongos , Diabetes Mellitus Experimental/tratamento farmacológico , Neovascularização Fisiológica/efeitos dos fármacos , Colágeno/química , Bandagens , Taninos/química , Taninos/farmacologia , Testes de Sensibilidade Microbiana , Staphylococcus aureus/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Masculino , Espécies Reativas de Oxigênio/metabolismo , Humanos , AngiogêneseRESUMO
Integrase plays an important role in the life cycle of HIV-1, and integrase strand transfer inhibitors (INSTIs) can effectively impair the viral replication. However, drug resistance mutations have been confirmed to decrease the efficacy of INSTI during the antiviral therapy. Herein, indole-2-carboxylic acid (1) was found to inhibit the strand transfer of integrase, and the indole nucleus of compound 1 was observed to chelate with two Mg2+ ions within the active site of integrase. Through optimization of compound 1, a series of indole-2-carboxylic acid derivatives were designed and synthesized, and compound 17a was proved to markedly inhibit the effect of integrase, with IC50 value of 3.11 µM. Binding mode analysis of 17a demonstrated that the introduced C6 halogenated benzene ring could effectively bind with the viral DNA (dC20) through π-π stacking interaction. These results indicated that indole-2-carboxylic acid is a promising scaffold for the development of integrase inhibitors.
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Hydrogel-based wearable flexible pressure sensors have great promise in human health and motion monitoring. However, it remains a great challenge to significantly improve the toughness, sensitivity and stability of hydrogel sensors. Here, we demonstrate the fabrication of hierarchically structured hydrogel sensors by 3D printing microgel-reinforced double network (MRDN) hydrogels to achieve both very high sensitivity and mechanical toughness. Polyelectrolyte microgels are used as building blocks, which are interpenetrated with a second network, to construct super tough hydrogels. The obtained hydrogels show a tensile strength of 1.61 MPa, and a fracture toughness of 5.08 MJ m-3 with high water content. The MRDN hydrogel precursors exhibit reversible gel-sol transitions, and serve as ideal inks for 3D printing microstructured sensor arrays with high fidelity and precision. The microstructured hydrogel sensors show an ultra-high sensitivity of 0.925 kPa-1, more than 50 times that of plain hydrogel sensors. The hydrogel sensors are assembled as an array onto a shoe-pad to monitor foot biomechanics during gaiting. Moreover, a sensor array with a well-arranged spatial distribution of sensor pixels with different microstructures and sensitivities is fabricated to track the trajectory of a crawling tortoise. Such hydrogel sensors have promising application in flexible wearable electronic devices.
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Hidrogéis , Microgéis , Humanos , Fenômenos Biomecânicos , Pé , Impressão TridimensionalRESUMO
Information encryption technologies are very important for security, health, commodity, and communications, etc. Novel information encryption mechanisms and materials are desired to achieve multimode and reprogrammable encryption. Here, a supramolecular strategy is demonstrated to achieve multimodal, erasable, reprogrammable, and reusable information encryption by reversibly modulating fluorescence. A butyl-naphthalimide with flexible ethylenediamine functionalized ß-cyclodextrin (N-CD) is utilized as a fluorescent responsive ink for printing or patterning information on polymer brushes with dangling adamantane group grafted on responsive hydrogels. The photoluminescent naphthalimide moiety is bonded to ß-CD and entrapped in the cavity. Its fluorescence is highly weakened in ß-CD cavity and recovers after being expelled from the cavity by a competing guest molecule to emit bright green photoluminescence under UV. Experiments and theoretical calculations suggest π-π stacking and ICT as the primary mechanism for the naphthalimides assembly and fluorescence, which can be quenched through insertion of conjugated molecules and recover by removing the insert. Such reversible quenching and recovering are used to achieve repeated writing, erasing, and re-writing of information. Supramolecular recognition and hydrogel shape memory are further combined to achieve reversible dual-encryption. This study provides a novel strategy to develop smart materials with improved information security for broad applications.
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OBJECTIVE: In order to solve the problem that the existing oxygen production technology cannot simultaneously produce pure oxygen, high-purity oxygen, ultra-pure oxygen, and the modular expansion of oxygen production capacity, a new type of electrochemical ceramic membrane oxygen production system was discussed and developed. METHODS: Through the design of the ceramic membrane stack, airflow distributor, heater, double spiral exchanger, thermal insulation sleeve, control panel, control box and auxiliary system in the electrochemical ceramic membrane oxygen generator, a modular oxygen production system is formed. RESULTS: The modular design can produce pure oxygen, high-purity oxygen and ultra-pure oxygen to meet various oxygen consumption needs. CONCLUSIONS: The electrochemical ceramic membrane oxygen production system is a new type of oxygen production technology. The main components have no moving parts, no noise, and no pollution. It can produce pure oxygen, high-purity oxygen and ultra-pure oxygen on site, with small size, light weight, and module combination which is suitable for convenient expansion and installation of oxygen consumption.
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Cerâmica , Oxigênio , TecnologiaRESUMO
Thermochromic smart windows are widely developed to modulate building energy exchange to save building energy consumption. However, most smart windows have fixed working temperatures, moderate energy-saving efficiency, and are not suitable for diverse (cold and hot) climates. Here smart windows with strong temperature modulation over a broad range of hydrogels with adjustable transition temperatures for all-weather building temperature regulation in different climates are reported. Thermochromic poly(N-isopropylacrylamide-co-N, N-dimethylacrylamide) hydrogels, with lower critical transition temperatures ranging from 32.5 to 43.5 °C, are developed for smart windows with solar modulation up to 88.84% and intrinsic transmittance up to 91.30% over full spectrum without energy input. Simulated indoor investigations are performed in different cities from 23 °N to 39 °N from winter to summer. The results indicate that smart windows have a strong solar modulation in summer to reduce indoor temperature up to 7.3 °C and efficient heat conservation in winter to save energy up to 4.30 J m-3 , in comparison to glass windows. Smart windows with grid patterns and Chinese kirigami are fabricated by using 3D printing of the hydrogels to achieve both solar modulation and light incidence. The strategy offers an innovative path for thermochromic smart windows for low carbon economy.
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Thermochromic smart windows are considered to be promising energy-saving devices for reducing energy consumption in buildings. The ideal materials for thermochromic smart windows should have high transmittance, high solar modulation, low phase-transition temperature, and excellent high-temperature thermal stability, which are difficult to achieve simultaneously. This work reports a simple one-step low-temperature polymerization method to prepare a thermo-responsive poly(N-isopropylacrylamide)/hydroxypropylmethyl cellulose (PNIPAM/HPMC) hydrogel achieving the above performances simultaneously. The low-temperature polymerization environment endowed the hydrogel with a high luminous transmittance (Tlum) of 90.82%. HPMC as a functional material effectively enhanced the mechanical properties and thermal stability of the hydrogel. Meanwhile, the PNIPAM/HPMC hydrogel showed a low phase-transition temperature (â¼32 °C) and high solar modulation (ΔTsol = 81.52%), which proved that it is an ideal material for thermochromic smart windows. Moreover, a PNIPAM/HPMC smart window exhibited high light transmittance (T380-760 = 86.27%), excellent light modulation (ΔT365 = 74.27%, ΔT380-760 = 86.17%, and ΔT940 = 63.93%), good indoor temperature regulation ability and stability, which indicated that it was an attractive candidate for application in reducing energy consumption in buildings. This work also provides an option and direction for modifying PNIPAM-based thermochromic smart windows.
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Natural gas hydrate has sparked worldwide interest due to its enormous energy potential. Geophysical surveys are commonly used in gas hydrate exploration, and resistivity logging plays an important role in this field. Nevertheless, the electrical response mechanism as a result of the gas hydrate growth in sediment is not well understood. This study develops an apparatus for the in situ resistivity testing of gas hydrate-bearing sediment combined with x-ray computed tomography scanning. Using this equipment, the gas hydrate samples can be synthesized under high-pressure and low-temperature conditions. The sample resistivities of three different layers can also be measured in situ during the gas hydrate formation. Moreover, x-ray computed tomography scanned gray images are acquired, which can be used to calculate the saturation and analyze the microscopic distribution of gas hydrate. A series of experiments are performed to validate the feasibility of the apparatus. The results show that the sample resistivity shows three distinct stages of variation as the gas hydrate grows. The most sensitive saturation range to the electrical response is â¼10.50%-22.34%. Very few gas hydrate particles will not significantly change the pore connectivity. By contrast, too many gas hydrate particles will hinder the pore network blocking. Both situations will not result in a significant change in resistivity.
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As one of the most important features of myocardial ischemia reperfusion (MI/R) injury, the overproduction of reactive oxygen species (ROS) overwhelms the intrinsic antioxidant and impairs the function of mitochondria and, finally, leads to cardiomyocyte death. To improve the damage of cardiomyocyte caused by oxidative stress, a series of α-carboline derivatives were designed and synthesized in this study. The biological studies revealed that most of the α-carbolines exhibited obvious protective activities against H2O2-induced cardiomyocyte injury. Among them, compound 14b significantly increased the cell viability in H2O2-induced oxidative stress in H9c2 cardiomyoblasts with a concentration-dependent manner, which was more potent than polydatin. Pretreatment of 14b obviously inhibited H2O2-induced lactate dehydrogenase (LDH) leakage, enhanced the capacity of endogenous antioxidant defenses, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and reduced the formation of the toxic product of lipid peroxidation (malondialdehyde, MDA). In addition, 14b effectively reduced the overproduction of ROS and restored the mitochondrial membrane potential ΔΨm, better than that of polydatin. Flow cytometry analysis demonstrated that 14b markedly reduced both necrosis and apoptosis in H9c2 cells after the exposure to H2O2. Further Western blot analysis revealed that 14b obviously decreased the ratio of Bax/Bcl-2 and reduced the expression of cytochrome c. Overall, these results revealed the potential of α-carboline 14b as a promising cardioprotective agent against H2O2-induced oxidative injury.
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Traumatismo por Reperfusão Miocárdica , Miócitos Cardíacos , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Apoptose , Carbolinas/metabolismo , Carbolinas/farmacologia , Humanos , Peróxido de Hidrogênio/metabolismo , Peróxido de Hidrogênio/farmacologia , Traumatismo por Reperfusão Miocárdica/tratamento farmacológico , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismoRESUMO
Long-term herbicide application may facilitate the adaptive evolution of weed populations. With Echinochloa crus-galli var. crus-galli population A from a rice field used for the experiment of effectiveness of herbicide in Nanling County, Anhui Province, we conducted common garden experi-ments with seeds of population A and three control populations collected from normal rice fields. Compared with the three control populations, population A had significantly lower seed production for individual plant, but higher 1000-seed weight. Population A had faster in seedling growth, higher in number of reproductive tillers, shorter life span, lower in height and biomass of individual plant, as well as lower in sensitivity to herbicide penoxsulam. Individuals from population A survived from 2× label dose (60 g·hm-2) of penoxsulam treatment at the three- to four-leaf stage showed significantly reduction in plant height, biomass, and mature seed production (1066 seeds per plant), but no difference in heading period, number of reproductive tillers, number of seeds per raceme and 1000-seed weight. The short lifespan, heavy seeds, dwarf architecture, more reproductive tillers and penoxsulam resistance made E. crus-galli var. crus-galli population A extremely adapting to rice planting systems, which should be prevented to spread to normal rice fields.
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Echinochloa , Herbicidas , Características de História de Vida , Oryza , Herbicidas/farmacologia , Humanos , SementesRESUMO
Facile preparation of organohydrogel electrolyte integrated with good anti-freezing property, toughness, transparency, conductivity and thermoplasticity is important and still remains challenging. Novel conductive and tough poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) composite organohydrogel electrolytes were obtained by a simple method in this paper. PVA and SA was firstly dissolved in a mixed solution of distilled water and glycerol and the PVA/SA/Gly organohydrogel was obtained by the freezing-thawing process, then PVA/SA/Gly organohydrogel was immersed into the saturated NaCl aqueous solution. During the soaking process NaCl would enter into the PVA/SA/Gly organohydrogel to increase the gel strength and conductivity. The PVA/SA/Gly organohydrogel electrolytes performed the high toughness with the tensile strength and elongation at break of 1.43 MPa and 558%, respectively. Moreover, the PVA/SA/Gly organohydrogel electrolytes behaved high transparency, anti-freezing property, conductivity and thermoplasticity due to the incorporation of glycerol. This paper provides a new preparation method for the high-performance organohydrogel electrolyte.
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Alginatos/química , Glicerol/química , Hidrogéis , Álcool de Polivinil/química , Hidrogéis/síntese química , Hidrogéis/químicaRESUMO
Hydrogels are important for stretchable and wearable multifunctional sensors, but their application is limited by their low mechanical strength and poor long-term stability. Herein, a conductive organohydrogel with a 3D honeycomb structure was prepared by integrating carbon nanotubes (CNTs) and carbon black (CB) into a poly(vinyl alcohol)/glycerol (PVA/Gly) organohydrogel. Such a nanocomposite organohydrogel is built on a physical cross-linking network formed by the hydrogen bonds among PVA, glycerol, and water. CNTs and CB had an add-in synergistic impact on the mechanical and electrical performances of the PVA/Gly organohydrogel because of the distinct aspect ratios and geometric shapes. The prepared organohydrogel integrated with a tensile strength of 4.8 MPa, a toughness of 15.93 MJ m-3, and flexibility with an elongation at break up to 640%. The organohydrogels also showed good antifreezing feature, long-term moisture retention, self-healing, and thermoplasticity. Sensors designed from these organohydrogels displayed high stretching sensitivity to tensile strain and temperature, with a gauge factor of 2.1 within a relatively broad strain range (up to â¼600% strain), a temperature coefficient of resistance of -0.935%·°C-1, and long-term durability. The sensors could detect full-range human physiological signals and respond to the change in temperature, which are highly desired for multifunctional wearable electronic devices.
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Facile preparation of super tough hydrogels with low temperature tolerance and anti-swelling property is still a challenging task for researchers. Meanwhile, the vast majority of tough hydrogels were obtained though chemical crosslinking and complicated synthesis or processing method accompanying a large number of harmful chemical reagents. Herein, the super tough chitosan/poly(vinyl alcohol) (CS/PVA) hydrogels (the maximum compressive strength of 18.97â¯MPa at a strain of 80% and the maximum tensile strength of 4.02â¯MPa at a strain of 406.4%) were successfully prepared via a simple post-treatment method. CS/PVA hydrogels were firstly prepared by freezing-thawing process and then soaking in saturated sodium chloride aqueous solution. The resultant hydrogels possess excellent swelling resistance and low temperature tolerance. This work shows that the post-treatment of immersing in saline solution is a feasible way to prepare super tough hydrogels with low temperature tolerance and anti-swelling property. This also would enlarge the application areas of CS.
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Quitosana/química , Hidrogéis/química , Fenômenos Mecânicos , Álcool de Polivinil/química , TemperaturaRESUMO
Leukemia remains a fatal disease for most patients and effective therapeutic strategies are urgently required. Typhaneoside (TYP) is a major flavonoid in the extract of Pollen Typhae, showing significant biological and pharmacological effects. In the present study, we explored the effects of TYP on acute myeloid leukemia (AML) progression. The results indicated that TYP markedly reduced the cell viability of AML cells and arrested the cell cycle at the G2/M phase by regulating the expression of associated proteins. In addition, TYP significantly induced apoptosis in AML cells by promoting the activation of Caspase-3. Intracellular and mitochondrial reactive oxygen species (ROS) accumulation were highly detected in AML cells after treatment with TYP. Moreover, TYP clearly induced ferroptosis in AML cells, and this process was iron-dependent and attendant with mitochondrial dysfunction. We also found that TYP significantly triggered autophagy in AML cells by promoting the activation of AMP-activated protein kinase (AMPK) signaling, contributing to ferritin degradation, ROS accumulation and ferroptotic cell death ultimately. In conclusion, the findings above provided solid evidences that TYP could be a promising therapeutic agent to prevent AML progression by inducing apoptosis, ROS production, autophagy and ferroptosis.
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Autofagia/efeitos dos fármacos , Ferroptose/efeitos dos fármacos , Glicosídeos/farmacologia , Leucemia Mieloide Aguda/tratamento farmacológico , Animais , Apoptose , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular , Células HL-60 , Humanos , Células K562 , Masculino , Potencial da Membrana Mitocondrial , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Transplante de Neoplasias , Espécies Reativas de Oxigênio/metabolismoRESUMO
BACKGROUND: Alopecurus japonicus is a serious grass weed species in wheat fields in eastern Asia, and has evolved strong resistance to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Although target-site resistance (TSR) to ACCase inhibitors in A. japonicus has been reported, non-target site resistance (NTSR) has not. This study investigated both TSR and NTSR in a fenoxaprop-P-ethyl-resistant A. japonicus population (AHFD-3), which was collected in Feidong County, Anhui Province, China. RESULTS: We found that AHFD-3 exhibited high resistance to fenoxaprop-P-ethyl and low resistance to flucarbazone-sodium. The sensitivity of AHFD-3 to fenoxaprop-P-ethyl increased significantly after treatment with cytochrome P450 (P450) inhibitors; however, such synergies between P450 inhibitors and fenoxaprop-P-ethyl were not found in two control populations. Sequences of the entire carboxyltransferase domain of A. japonicus ACCase were obtained, and AHFD-3 plants showed an Asp-2078-Gly substitution in the ACCase. With the derived cleaved amplified polymorphic sequence (dCAPS) method, we found that 85.4% of the plants of AHFD-3 carried this mutation. The P450 content in AHFD-3 plants was significantly higher than those of the two control populations after treatment with fenoxaprop-P-ethyl. Ten partial sequences of P450 genes in A. japonicus were cloned. Three P450 genes were up-regulated 12 h after fenoxaprop-P-ethyl treatment, which were all from the P450 subfamily CYP72A. Moreover, a P450 gene from the P450 family CYP81 was up-regulated after fenoxaprop-P-ethyl treatment in all populations studied. CONCLUSION: Fenoxaprop-P-ethyl resistance in AHFD-3 plants was conferred by up-regulation of cytochrome P450s in the CYP72A subfamily and target site mutation of the ACCase gene. © 2018 Society of Chemical Industry.
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Acetil-CoA Carboxilase/genética , Sistema Enzimático do Citocromo P-450/genética , Resistência a Herbicidas/genética , Oxazóis/farmacologia , Proteínas de Plantas/genética , Poaceae/efeitos dos fármacos , Poaceae/genética , Propionatos/farmacologia , Acetil-CoA Carboxilase/metabolismo , China , Sistema Enzimático do Citocromo P-450/metabolismo , Herbicidas/farmacologia , Mutação , Proteínas de Plantas/metabolismoRESUMO
Echinochloa phyllopogon proliferation seriously threatens rice production worldwide. We combined a restriction-site associated DNA (RAD) approach with Illumina DNA sequencing for rapid and mass discovery of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for E. phyllopogon. RAD tags were generated from the genomic DNA of two E. phyllopogon plants, and sequenced to produce 5197.7 Mb and 5242.9 Mb high quality sequences, respectively. The GC content of E. phyllopogon was 45.8%, which is high for monocots. In total, 4710 putative SSRs were identified in 4132 contigs, which permitted the design of PCR primers for E. phyllopogon. Most repeat motifs among the SSRs identified were dinucleotide (>82%), and most of these SSRs were four motif-repeats (>75%). The most frequent motif was AT, accounting for 36.3%-37.2%, followed by AG and AC. In total, 78 putative polymorphic SSR loci were found. A total of 49,179 SNPs were discovered between the two samples of E. phyllopogon, 67.1% of which were transversions and 32.9% were transitions. We used eight SSRs to study the genetic diversity of four E. phyllopogon populations collected from rice fields in China and all eight loci tested were polymorphic.
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Arable areas are commonly susceptible to alien plant invasion because they experience dramatic environmental influences and intense anthropogenic activity. However, the limited reports on relevant factors in plant invasion of croplands have addressed single or a few invasive species and environmental factors. To elucidate key factors affecting plant invasions in croplands, we analyzed the relationship between 11 effective factors and changes in composition of alien plants, using field surveys of crop fields in Anhui Province conducted during 1987-1990 (historical dataset) and 2005-2010 (recent dataset), when rapid urbanization was occurring in China. We found that in the past few decades, the dominance and richness of alien plant populations approximately doubled, despite differences among the 4 regions of Anhui Province. Among the 38 alien invasive plant species observed in the sites, the dominance values of 11 species increased significantly, while the dominance of 4 species decreased significantly. The quantity of chemical fertilizer and herbicide applied, population density, agricultural machinery use, traffic frequency, and annual mean temperature were significantly related to increased richness and annual dominance values of alien plant species. Our findings suggest that the increase in alien plant invasions during the past few decades is primarily a result of increased application of chemical fertilizer and herbicides.