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1.
J Biomed Mater Res B Appl Biomater ; 95(1): 161-70, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20737557

RESUMO

A variety of nerve conduits incorporated with chemical and biological factors have been developed to further stimulate nerve regeneration. Although most of the nerve guides in studies are basically limited to bridge a short gap of nerve defect in rat models, it is vital to evaluate effects of conduits on nerve regeneration over distance greater than 20 mm, or more clinically relevant nerve gap lengths in higher mammals. In this study, a poly(lactide-co-glycolide) (PLGA) nerve conduit, treated with pulsed plasma and coated with ciliary neurotrophic factor (CNTF) as well as chitosan, was used to repair 25-mm-long canine tibial nerve defects in eighteen cross-bred dogs. The canines were randomly divided into three groups (n = 6), a 25-mm segment of the tibial nerve was removed and replaced by a PLGA/chitosan-CNTF nerve conduit, PLGA/chitosan conduit and autologous nerve grafts were performed as the control. The results were evaluated by general observation, electromyogram testing, S-100 histological immunostaining, and image analysis at 3 months after operation. The histological results demonstrated that the PLGA/chitosan-CNTF conduits and PLGA/chitosan conduits were capable of leading the damaged axons through the lesioned area. Through the comparison of the three groups, the results in PLGA/chitosan-CNTF conduits group were better than that of PLGA/chitosan conduits group, while they were similar to autologous nerve grafts group. Therefore, CNTF-coated PLGA/chitosan nerve conduits could be an alternative artificial nerve conduit for nerve regeneration.


Assuntos
Quitosana , Fator Neurotrófico Ciliar , Regeneração Tecidual Guiada/métodos , Ácido Láctico , Regeneração Nervosa/efeitos dos fármacos , Ácido Poliglicólico , Nervo Tibial/fisiologia , Animais , Materiais Revestidos Biocompatíveis/química , Cães , Teste de Materiais , Nervos Periféricos/fisiologia , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Nervo Tibial/lesões
2.
Neuroreport ; 15(14): 2167-72, 2004 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-15371726

RESUMO

Autografts have been extensively studied to facilitate optic nerve (ON) regeneration in animal experiments, but the clinical application of this approach to aid autoregeneration has not yet been attempted. This study aims to explore the guided regeneration by an artificial polyglycolic acid-chitosan conduit coated with recombinant L1-Fc. Consistent with previous studies; in vitro assay showed that both chitosan, a natural biomaterial, and the neural cell adhesion molecule L1-Fc enhanced neurite outgrowth. Rat optic nerve transection was used as an in vivo model. The implanted PGA-chitosan conduit was progressively degraded and absorbed, accompanied by significant axonal regeneration as revealed by immunohistochemistry, anterograde and retrograde tracing. The polyglycolic acid-chitosan conduit coated with L1-Fc showed more effective to promote axonal regeneration and remyelination. Taken together, our observations demonstrated that the L1-Fc coated PGA-chitosan conduits provided a compatible and supportive canal to guild the injured nerve regeneration and remyelination.


Assuntos
Quitosana/uso terapêutico , Regeneração Nervosa/efeitos dos fármacos , Molécula L1 de Adesão de Célula Nervosa/uso terapêutico , Traumatismos do Nervo Óptico/tratamento farmacológico , Ácido Poliglicólico/uso terapêutico , Animais , Materiais Biocompatíveis/uso terapêutico , Linhagem Celular Tumoral , Feminino , Regeneração Nervosa/fisiologia , Nervo Óptico/citologia , Nervo Óptico/efeitos dos fármacos , Nervo Óptico/fisiologia , Traumatismos do Nervo Óptico/patologia , Ratos , Ratos Wistar , Proteínas Recombinantes/uso terapêutico
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