RESUMO
The dysregulation of miR-532-5p is involved in the development of several cancers. Nevertheless, the roles of miR-532-5p in osteosarcoma (OS) have yet to be illuminated. In the present study, we found that miR-532-5p was significantly downregulated in both OS tissues and cell lines. The low level of miR-532-5p was associated with advance clinical stage and poor overall survival in patient with OS. The functional experiments implied that upregulation of miR-532-5p restrained OS U2OS cell growth and metastatic ability in vitro; induced apoptosis, and impaired OS cell growth in vivo. Mechanistically, chemokine (C-X-C Motif) ligand 2 (CXCL2) was proved as a target gene of miR-532-5p. The inhibitory effects of miR-532-5p on OS cell were rescued by CXCL2 overexpression. Altogether, we demonstrated that miR-532-5p exerted tumor-inhibitory functions in OS cell via regulating CXCL2.
Assuntos
Neoplasias Ósseas/genética , Quimiocina CXCL2/genética , MicroRNAs/genética , Osteossarcoma/genética , Adolescente , Adulto , Antagomirs/genética , Antagomirs/metabolismo , Apoptose/genética , Pareamento de Bases , Sequência de Bases , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/metabolismo , Neoplasias Ósseas/mortalidade , Osso e Ossos/metabolismo , Osso e Ossos/patologia , Linhagem Celular Tumoral , Proliferação de Células , Quimiocina CXCL2/agonistas , Quimiocina CXCL2/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Metástase Linfática , Masculino , MicroRNAs/agonistas , MicroRNAs/antagonistas & inibidores , MicroRNAs/metabolismo , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Oligorribonucleotídeos/genética , Oligorribonucleotídeos/metabolismo , Osteossarcoma/diagnóstico , Osteossarcoma/metabolismo , Osteossarcoma/mortalidade , Fenótipo , Plasmídeos/química , Plasmídeos/metabolismo , Prognóstico , Transdução de Sinais , Análise de SobrevidaRESUMO
To investigate the clinical efficacy of antibiotic impregnated beads on the tibial chronic osteomyelitis, so as to search for a more applicable method for the treatment. Through comparative analysis, we divided 72 patients with chronic tibial osteomyelitis who received treatment in hospital between January 2016 and December 2016 randomly into two groups: Control group (n=36) and the experiment group (n=36). Patients in the control group underwent closed lavage plus drainage for treatment, while those in the experiment group received the antibiotic impregnated beads. After treatment, we compared the times of treatment, average length of stay in hospital and the efficacy between two groups, and data were analyzed using SPSS 15.0 software. In the control group, average length of stay in hospital was ((3.3±0.9) months, average time of surgery was (2.9±1.8) times, cure rate was 25.0% and elimination rate of bacteria was 88.0%; in the experiment group, average length of stay in hospital was ((2.2±1.3) months, average time of surgery was (2.4±1.0) times, cure rate was 47.2%, and elimination rate of bacteria was 93.8%. Differences in the average length of stay in hospital, the cure rate and elimination rate of bacteria between two groups showed statistical significance (p<0.05). For tibial chronic osteomyelitis, antibiotic impregnated bead implantation can reduce the chance of secondary infection after operation and shorten the hospitalization time, showing a more promising effect than the closed lavage and drainage, and this method is worthy of being promoted in clinical practice.