Kidney biopsy in subsaharan Africa / Ponctions biopsies rénales en Afrique subsaharienne

Materials and methods: We carried out a retrospective and descriptive study on biopsies examined between January 2015 and December 2019, in the pathological departments of University Teaching Hospital of Bouaké and Cocody-Abidjan. The KB came from four countries (Côte d'Ivoire, Togo, Guinea-Conakry and Burkina Faso). Optical microscopy and/or direct immunofluorescence techniques were used. All biopsy samples including epidemiological, clinical and pathological data and an optical microscopy and/or direct immunofluorescence study were included. The parameters studied were indications for KB, epidemiological profile, clinic, proteinuria and pathological aspects. Results: Over the study period, we collected 179 KB, i.e. 35.8 KB/year. The mean age of the patients was 32.9 ±13.8 years (range 11-70 years). The sex ratio (M/F) was 1.03. Pure nephrotic syndrome was the main indication (64.2 %, n = 115) for KB, followed by impure nephrotic syndrome (11.7 %, n = 21), acute renal failure (ARF) (7.8 %, n = 14) and rapidly progressive glomerulonephritis (RPGN) (7.8 %, n = 14). Glomerulonephritis (GN) occurred in 86 % (n = 158), vascular nephropathy in 11.7 % (n = 21) and tubulointerstitial nephritis in 2.2 % (n = 4). The nephropathies were preferentially focal segmental glomerulosclerosis (34.6 %, n = 62), nephroangiosclerosis (10.6 %, n = 19), membranous GN (10 %, n = 18), post-infectious GN (8.9 %, n = 16) and lupus GN (7.3 %, n = 13). Conclusion: The KB is an essential step in the diagnosis of nephropathies. Focal segmental glomerulosclerosis is frequent in our study. The establishment of a Kidney registry would allow better knowledge of renal pathologies in sub-Saharan Africa.

La ponction biopsie rénale (PBR) constitue une avancée notable dans la prise en charge des néphropathies. En Afrique subsaharienne, peu d'études ont été réalisées. L'objectif de notre travail était d'évaluer les indications de la PBR et de déterminer les caractéristiques épidémiologiques et histologiques des néphropathies diagnostiquées en Afrique subsaharienne. Matériels et méthodes: Nous avons mené une étude rétrospective et descriptive portant sur les PBR examinées entre janvier 2015 et décembre 2019, dans les services d'anatomie et cytologie pathologiques des CHU de Cocody-Abidjan et de Bouaké. Les PBR provenaient de quatre pays africains (Côte d'Ivoire, Togo, Guinée-Conakry et Burkina Faso). Les techniques de microscopie optique et/ou d'immunofluorescence directe ont été utilisées. Nous avons inclus l'ensemble des PBR contributives sur cette période et pour lesquelles nous disposions de données cliniques et biologiques. Les paramètres étudiés étaient les données cliniques et biologiques, l'indication de la PBR et les résultats histologiques. Résultats: Sur la période d'étude, nous avons colligé 179 PBR, soit 35,8 PBR/an. L'âge moyen des patients était de 32,9 ± 13,8 ans (extrêmes de 11 à 70 ans). Le sex ratio (H/F) était de 1,03. Le syndrome néphrotique pur était la principale indication (64,2 %, n = 115) à la réalisation d'une PBR, suivi du syndrome néphrotique impur (11,7 %, n = 21), de l'insuffisance rénale aiguë (IRA) (7,8 %, n = 14) et de la glomérulonéphrite rapidement progressive (GNRP) (7,8 %, n = 14). Les glomérulonéphrites (GN) s'observaient dans 86 % des cas (n = 158), les néphropathies vasculaires dans 11,7 % (n = 21) et les néphrites tubulo-interstitielles dans 2,2 % (n = 4). Les néphropathies les plus fréquentes étaient la hyalinose segmentaire et focale (34,6 %, n = 62), la néphroangiosclérose (10,6 %, n = 19), la GN extramembraneuse (10 %, n = 18), la GN post-infectieuse (8,9 %, n = 16) et la GN lupique (7,3 %, n = 13). Conclusion: La PBR est un geste capital pour le diagnostic des néphropathies. La hyalinose segmentaire et focale est la principale nosologie retrouvée dans notre cohorte. La mise en place d'un registre Rein permettrait une meilleure connaissance et prise en charge des pathologies rénales en Afrique subsaharienne.

Predictive factors of difficult biliary cannulation: An experience of a tunisian tertiary center.

Introduction: Selective biliary cannulation is a prerequisite for a successful endoscopic retrograde cholangiopancreatography (ERCP). However, conventional biliary access can be difficult. The aims of our study were to determine the prevalence of difficult biliary cannulation (DBC) and its associated factors and to describe the efficiency and safety of used standard and advanced cannulation techniques. Methods: We conducted a single-center retrospective study including all patients with naïve papilla who had an ERCP procedure in Gastroenterology department of Mohamed Taher Maamouri Hospital from June 2019 to December 2021. Efficiency was defined as successful selective deep biliary cannulation. DBC was defined based on the presence of one or more of the European Society of Gastrointestinal Endoscopy (ESGE) criteria (5-5-1): more than five cannulation attempts, more than 5 min before cannulation and more than one accidental passage in the wirsung. Prevalence was measured using ESGE 5-5-1 cutoffs and chinese set cutoffs 15-10-2. Predictors of DBC were sought by univariate and multivariate analysis (SPSS software, p significant if < 0.05). Results: We included 664 patients (mean age 62 years and sex ratio M/W = 0.8). Main indication for ERCP was choledocholithiasis (67%, n = 442) followed by malignant biliary stenosis (21%, n = 138). Based on ESGE criteria, prevalence of DBC was 42.62% (n = 283). Prevalence was 21.15% when 15-10-2 cutoffs are applied in trainee-involved procedure. Cumulative biliary success rate was 96.46%. Standard cannulation method achieved access in 98.2% while advanced methods permitted success in 92.2% in fistulotomy, 94.1% in papillotomy and 77.3% in transpancreatic sphincterotomy. Independent predictive factors of DBC in multivariate analysis were: Trainee presence OR 1.80 [1.24-2.65], SOD OR 4.71 [1.11-19.88], biliary stenosis found on imaging examinations (OR 2.53 [1.63-3.92], small papilla OR 4.09 [1.82-9.17] and difficult orientation of the papilla OR 14.90 [3.28-67.62]. Conclusion: DBC is a frequent endoscopic situation. Predictors of DBC can be related to trainee involvement in the procedure, anatomical and clinical factors. A thorough understanding of these factors can actively contribute to ERCP management plans.

[Efficacy of highly active antiretroviral therapy alone in the treatment of diffuse lymphocytic infiltration syndrome in an ivorian patient living with HIV: a case report]. / Efficacité du traitement antirétroviral hautement actif seul dans le traitement du syndrome d'infiltration lymphocytaire diffus chez une patiente ivoirienne vivant avec le VIH : à propos d'un cas.

Objective: We report in this work the efficacy of highly active antiretrovirals (ARVs) alone in the treatment of diffuse infiltrative lymphocytosis syndrome (DILS) without the use of corticosteroids, which appears risky in patients living with HIV. Observation: This is a 60-year-old HIV-positive patient, discovered during the etiological workup of renal failure, which revealed a non-nephrotic glomerular profile. The renal biopsy found an interstitial infiltrate of CD8 suggestive of DILS. Management consisted in starting ARV treatment alone (lamuvidine, abacavir and raltegravir) without associated corticosteroid therapy. The clinical evolution under treatment was marked by a recovery of the renal function with a creatininemia at 99 µmol/l, a regression of the proteinuria, a CD4 rate at 293/mm3 and an HIV viral load at 533.3 copies or 1.6 log in the space of 3 months. Conclusion: DILS is a diffuse systemic disease in HIV patients who are usually under poor virological control. In view of the strong immunosuppression and the absence of other infiltrative diseases, it appeared to us to be risky and unjustified to add a corticosteroid therapy.

[Dyslipidemia in uremic patients at the National Hospital of Donka-Conakry]. / Profil lipidique des urémiques a l'Hôptal National de Donka à Conakry.

The prevalence of the dyslipidemia is higher at the patients in chronic renal failure (CRF) than in the general population. The objective of this study was to determine the prevalence and the lipidic anomalies among uraemic patients and to evaluate the others associated factors of cardiovascular risk. The study was descriptive with prospective recruitment (November 2004-June 2005); concerned 60 patients having an advanced chronic renal failure whose calculated clearance of creatinin was lower than 30 ml/mn as well as the hemodialysed patients. The lipidic disturbances on the whole 9/16 dialysed (56%) and 31 uraermia /44 were not dialysed (70%). This majority triglyceride dyslipidemia comprised 8 cases of hypertriglyceridemia in the group of dialysed patients against 19 in the group of not dialysed; 1 case of mixed hyperlipidemia against 6 cases and 4 cases of hypercholesterolemia HDL in the non dialysed group with 1 case of HDL low cholesterol. The patients with dyslipidemia were 4 females (25%) and 5 males (31%) among dialysed group; and of 13 female (29%) and 18 male (41%) among not dialysed. The average age was of 54.3 +/- 0.7 years for dialysed against the 46.6 +/- 0.2 years for the dyslipidémia in the non dialysed. Among others factors of cardiovascular risk, the arterial high blood pressure was noted at 9 dialysed patient's dyslipidémia and 20 case / 31 to the not dialysed dyslipidémia; and the diabetes was observed in 1 and 3 cases respectively. The addiction to smoking was noted in both groups except at the dialysis patients not dyalipidémiques.