Evaluating seasonal variations of fecal coliform colonization in Magallana hongkongensis and Crassostrea rhizophorae: A preliminary study of oyster quality grown in Hong Kong aquacultural farms.

The objective of this research is to investigate the spatial and temporal patterns of bacteriological quality in raw oysters sampled from different aquacultural farms located in Aberdeen Typhoon Shelter, Carp Gates, Lau Fau Shan, Ma Wan, and Mui Wo in Hong Kong. Magallana hongkongensis and Crassostrea rhizophorae were collected and analyzed for fecal coliforms. Throughout the 13-month monitoring period, all samples had generally high bacterial loads, ranging from 1.4 × 107 cfu/g to 8.9 × 107 cfu/g and exceeded the guideline suggested by the HKSAR government (i.e. 700 MPN/100 g). Besides, a linear regression analysis showed that the amount of fecal coliforms in raw oysters had strong correlations (p < 0.05) to the monthly rainfall records throughout the monitoring period. Such findings illustrate the high loading of pathogenic microorganisms in the tissue of oysters which represent a potential threat of people contracting foodborne diseases.

An assessment of heavy metal contamination in the marine soil/sediment of Coles Bay Area, Svalbard, and Greater Bay Area, China: a baseline survey from a rapidly developing bay.

The objective of this research is to investigate the pollution levels of tin (Sn), arsenic (As), cadmium (Cd), copper (Cu), lead (Pb), mercury (Hg), and zinc (Zn) of soil/sediment taken in Coles Bay Area (CBA), Svalbard, and Great Bay Area (GBA), China, in an attempt to evaluate the pollution potential related to recent development in the areas. A total of 150 soil/sediment samples were collected in each location. Heavy metal concentrations were detectable levels at all sites and the values of Cd of all soil/sediment samples were higher than Dutch Target and Intervention Values. Heavy metal concentration in soil/sediment was further analyzed by principal component analysis (PCA). It was revealed that three components were found in two studying sites and contributed 60.2% and 75.9% of variations to reflect soil/sediment quality in CBA and GBA, respectively. Based on the results of KMO (0.52) and Bartlett's test (p < 0.000), there are 32.1%, 15.6%, and 11.1% and 39.45%, 19.01%, and 17.52% of the variance in the first, second, and third component explained that the metal concentration of Pb, Cd, and Sn was highly correlated with the soil/sediment quality in CBA and GBA, respectively. Among these three heavy metals, Cd concentration was the common dominant factor to affect soil/sediment quality in these two study sites. It is recommended that investigation of the sources of pollution (either point or non-point source) during CBA or GBA development and management together with consideration of abiotic (soil)-biotic (organisms) interactions should be taken into account when choosing suitable remediation strategies in the future.

Feasibility of Sijunzi Tang (Chinese medicine) to enhance protein disulfide isomerase activities for reactivating malate dehydrogenase deactivated by polycyclic aromatic hydrocarbons.

The objective of this research is to investigate the enzymatic activities between protein disulfide isomerase (PDI) found in animals and plants and the properties found in a commonly used Chinese medicine called Sijunzi Tang. During the investigation, PDI, which is a monomer with a molecular mass of 57.0 kDa, was used to reactivate malate dehydrogenase (MDH). However, with the interference of polycyclic aromatic hydrocarbons (PAHs), evidence indicates that such chemicals are carcinogenic, mutagenic, and toxic to humans. The enzymatic activity of PDI found in animal's liver and plant was 1657 folds of purification; 0.284 unit/mg of enzyme activity, and 5694.4 folds of purification; 1.00 unit/mg of enzyme activity, respectively. PDI extracted in treated animal and plant tissue revealed 2.40% and 80.44% of regaining MDH enzymatic activity, respectively. Although in its initial phase of investigation, it is assumed that the properties found in Sijunzi Tang can help regain enzymatic activity in those affected by xenobiotic substances, thus, making it a potential ingredient in assisting with PDI functions.

Behavioural toxicity studies of Cyclope neritea and Nassarius mutabilis exposed to polycyclic aromatic hydrocarbons.

The objective of the work was to study behavioural change of Cyclope neritea (sea snail) and Nassarius mutabilis (land snail) upon exposure to different levels of PAHs. Snail's behaviour was translated and expressed in Behavioural State Score (BSS) where the score ranged from "0" to "5" points refers to the ascending level of locomotion of a snail. A significant difference was found in snail's behaviour in 25.0 mg/L than in 0.5 mg/L with p value smaller than 0.01. BSS scores appear most frequent on the treatment and control group were 5 (61.5-64.5%) and 2 (41.0-45.0%), respectively. Intersex behaviour was found in all species (i.e. the same sex was grouped together) regardless of PAH concentrations. This is the first reported to study the behavioural change of snail sampled in Hong Kong area when exposed to PAHs. Further studies should be carried on the impact of snail's behaviour exposure on each congener in the family of PAHs.

The potential of Mikania micrantha (Chinese creeper) to hyperaccumulate heavy metals in soil contaminated by electronic waste.

The objective of this study is to investigate the contributions of Mikania micrantha (chinese creeper) to remediate heavy metal pollutants present in an e-waste-contaminated soil. Different proportions of e-waste soil (0%, 25%, 50%, 75%, and 100% w/w) planted with Cynodon dactylon together with the test species were prepared for testing relative seed germination (RSG), relative root growth (RRG), and germination index (GI) tests. A significant higher value of GI (77.8%) was found in M. micrantha than that of the other species when planting in 100% e-waste-contaminated soil. A significant correlation (< 0.05) was found between heavy metal concentration and germination assays in M. micrantha. A significant decrease in heavy metal concentration of the polluted soil after the experiment indicated that biomolecule development studies to determine the aggregate benefit of M. micrantha for phytoremediation remain to be studied in future.

Spatial distribution of polycyclic aromatic hydrocarbons in soil, sediment, and combusted residue at an e-waste processing site in southeast China.

The environmental pollution and health impacts caused by the primitive and crude recycling of e-waste have become urgent global issues. Guiyu, China is a major hotspot of e-waste recycling. In this study, the levels and distribution of polycyclic aromatic hydrocarbons in soil in Guiyu were determined to investigate the effect of e-waste activities on the environment and to identify possible sources of these pollutants. Sediment samples from a local duck pond, water gullies, a river tributary, and combusted residue from e-waste burning sites were also investigated. The general trend found in soil (Σ16 PAHs) was acid leaching site > duck pond > rice field > printer roller dump site > reservoir (control site) and ranged from 95.2 ± 54.2 to 5,210 ± 89.6 ng/g (dry wt). The highest average total PAH concentrations were found in combusted residues of wires, cables, and other computer electrical components located at two e-waste open burning sites (18,600 and 10,800 ± 3,940 ng/g). These were 195- and 113-fold higher than the PAH concentrations of soil at the control site. Sediment PAH concentrations ranged from 37.2 ± 6 to 534 ± 271 ng/g. Results of this study provide further evidence of significant input of PAHs to the environment attributed to crude e-waste recycling.

Contamination and risk assessment (based on bioaccessibility via ingestion and inhalation) of metal(loid)s in outdoor and indoor particles from urban centers of Guangzhou, China.

Road dust, household air-conditioning (AC) filter dust and PM2.5 were collected to investigate the contamination of metal(loid)s (Cr, Mn, Ni, Cu, Zn, As, Cd, Sn, Sb, Hg and Pb) in outdoor and indoor urban environments of Guangzhou. Zinc was found to be the most abundant element in road dust and household PM2.5, while the concentration of Pb was the highest in AC filter dust. Enrichment factor (EF) was used to assess the influence of human activity on the contamination of these metal(loid)s. Ingestion and inhalation were the two exposure pathways applied for risk assessment. Physiologically based extraction test (PBET) was used to estimate the oral bioaccessibilities of metal(loid)s in road dust and AC filter dust. Respiratory bioaccessible fraction of metal(loid)s via household PM2.5 was extracted with lung simulating solution. Household AC filter dust was more hazardous to human health than road dust, especially to children. Arsenic was found to be the most risky element based on the risk assessment.

Size fraction effect on phthalate esters accumulation, bioaccessibility and in vitro cytotoxicity of indoor/outdoor dust, and risk assessment of human exposure.

Indoor and outdoor dusts from two urban centers in the Pearl River Delta, China, were analyzed and phthalate esters varied from 4.95 to 2,220 µg g(-1) in indoor dust, significantly higher than outdoor dust (1.70-869 µg g(-1)). Di-2-ethylhexyl phthalate (DEHP) was the dominant phthalate found and the highest distribution factor (DF) (1.56 ± 0.41) was noted in the <63 µm fraction (p<0.05). In vitro cytotoxicity of dust extract on human T cell lymphoblast leukemic cell line (CCRF-CEM) indicated by Lethal Concentration 50 (LC50) decreased with particle size. The power model was found as a better fit for explaining the relationship between LC50 and phthalates (R(2)=0.46, p<0.01). Bioaccessibility of phthalates in dust varied with different particle sizes, with the greatest bioaccessible fraction (2.49-38.6%) obtained in <63 µm. Risk assessment indicated that indoor dust ingestion accounted for the major source for DEHP exposure (81.4-96.4% of non-dietary exposure and 36.5% of total exposure), especially for toddlers. The cancer risks associated with DEHP via home dust were high (10(-6)-10(-4)), with 10% of houses estimated with unacceptable risks (>10(-4)). After corrected with the bioaccessibility of phthalates, the cancer risks of dust exposure were moderate (10(-7)-10(-5)).

Exposure assessment and distribution of polychlorinated biphenyls (PCBs) contained in indoor and outdoor dusts and the impacts of particle size and bioaccessibility.

The size fraction, bioaccessibility and associated human daily intake of PCBs via indoor and outdoor dust collected from two most populated urban centers of Pearl River Delta (PRD), China, were studied. The ΣPCBs levels (ng g(-1)) in indoor (51.9-264) and outdoor (4.02-228) dust in Guangzhou (GZ) were found higher than those in indoor (17.4-137) and outdoor (7.75-114) dust of Hong Kong (HK). Hexa-PCB was the largest contributor in dust samples (29-64%), followed by tri-PCB. The size fraction of PCBs indicated a high accumulation effect of particles less than 63 µm, while the lowest was found in 280-2000 µm. Toxic equivalency (TEQ) of dioxin-like PCBs in indoor dust of GZ and HK was 2 to 13 times higher than that in outdoor dust. The bioaccessibility of PCBs was determined as 5-61% depending on individual PCB congeners under study and bioaccessible PCB exposure was significantly lower than the estimate for total PCB. The daily intake of bioaccessible PCBs via dust ranged in 0.02-8.95 and 0.37-17.8 ng day(-1) in GZ while 0.01-4.95 and 0.16-9.83 ng day(-1) in HK for adults and children, respectively. Dust ingestion contributed to 0.49-10.6% of overall non-dietary PCB exposure (dust ingestion and inhalation) for adults while 12.9-35% for children, indicating the dominant contribution from inhalation.

Risk assessment of human exposure to bioaccessible phthalate esters via indoor dust around the Pearl River Delta.

There is limited information on the bioaccessible fractions of phthalate esters in indoor dust in order to estimate human exposure. In the present study, workplace dust and settled house dust samples from Hong Kong, Shenzhen, and Guangzhou, the three major cities scattered around the Pearl River Delta (PRD) were collected. Chemical analyses showed that the phthalates in workplace dust ranged from 144 to 1810 µg/g, with dust from shopping malls containing the highest level, and in home dust ranged from 181 to 9240 µg/g. The most abundant phthalate ester found was bis(2-ethylhexyl) phthalate (DEHP) in both workplace dust and home dust, followed by di-n-butyl phthalate (DBP) and di-iso-butyl phthalate (DIBP). Principal Components Analysis (PCA) indicated that indoor dust around PRD showed similar phthalate esters patterns of composition. A significant correlation was observed between total phthalate esters concentrations in home dust and the number of year of house construction (p < 0.05). The oral bioaccessibility of phthalate esters in indoor dust ranged from 10.2% (DEHP) to 32% (DMP). Risk assessment indicated that the dominant exposure routes varied in different phthalate esters exposure profiles and the dermal contact exposure pathway was identified as an important route for indoor DEHP exposure.

Mercury levels in road dust and household TSP/PM2.5 related to concentrations in hair in Guangzhou, China.

Road dust, household total suspended particulate matters (TSP) and PM2.5 were collected in urban area of Guangzhou, south of China, to investigate the concentrations of total mercury (THg) and methyl mercury (MeHg). The household PM2.5 concentrations varied between 16.2 and 623 µg/m³. The average PM2.5 level (174 µg/m³) from all of the locations exceeded 24-h concentration from WHO air quality guideline The average concentrations of THg and MeHg were: 235 µg/kg and 0.392 µg/kg in road dust, 600 µg/kg and 1.49 µg/kg in TSP; 1006 µg/kg (104 pg/m³) and 1.40 µg/kg (0.134 pg/m³) in PM2.5. Risk assessment showed that the Hazardous quotients (HQs) of exposure to Hg and MeHg via road dust and TSP were less than 1, indicating that no adverse risk was manifested. Ingestion of dust was found to constitute a relatively minor pathway of Guangzhou residents' exposure to Hg. Furthermore, human hair samples from 88 Guangzhou citizens were also analyzed to investigate the mercury accumulation in human body in Guangzhou. The average concentrations of THg and MeHg in human hair samples were 869±831 µg/kg and 104±108 µg/kg respectively. However, no significant correlations of the mercury species were noted between human hair and road dust, TSP and PM2.5.

Biological analysis of endocrine-disrupting chemicals in animal meats from the Pearl River Delta, China.

Endocrine disrupting chemicals (EDCs) consist of a diverse group of industrial chemicals and pharmacological agents. The use of instrumental analyses as the first screening tool might not be cost-effective to identify the existence of enormous numbers of chemical contaminants in environments. Also, knowledge of the concentration of individual residues is difficult to use to evaluate biological impacts of contaminants to wildlife and humans. The primary objective of the present study was to develop and to test the feasibility of using a battery of exposure biomarkers for the rapid-screening of various endocrine disrupting activities present in food. The measurement of the EDC-elicited activities involved various (i) receptor-mediated responses, including androgenic, estrogenic, dioxin-like, glucocorticoid-like, progesterone-like, peroxisome proliferator-like and retinoid-like as well as (ii) the non-receptor mediated responses through modulation of cellular reactive oxygen species (ROS) and ATP content. Samples of both local and imported pork, beef and chicken as well as freshwater and seawater fishes were collected. Extracts of different foods exhibited various dioxin-like and "hormonal" activities. Fish and chicken skin were found to be the major source of exogenous "hormonal" and dioxin-like substances in diets. Extracts of beef and pork contained lesser potencies of hormonally-active agents. Our data suggest that the proposed EDC-screening platform may be useful in a risk assessment for the routine monitoring of EDCs in foods. Continuous monitoring and research is warranted to assess the physiological consequences of the consumption.

Polycyclic aromatic hydrocarbons (PAHs) in urban surface dust of Guangzhou, China: Status, sources and human health risk assessment.

Ninety-six urban surface dust samples collected from Guangzhou, a typical urban center in South China, were analyzed for 16 PAHs (2-6 rings). ∑PAHs concentrations in the urban surface dust ranged from 0.84 to 12.3µg/g with a mean of 4.80µg/g. High molecular weight compounds (4-6 rings) contributed to 62 to 94% of ∑PAHs mass in the surface dust samples. Four hotspots with highest ∑PAHs were identified via kriging prediction mapping, representing the highly-urbanized regions: central downtown, highway and industrial area. Two major origins of PAHs inputs to urban surface dust were identified as vehicle emissions (51.9%) and coal combustion (26.8%). The 95% UCL of Incremental Lifetime Cancer Risk (ILCR) due to human exposure to urban surface dust PAHs in central South China was 3.03×10(-6) for children and 2.92×10(-6) for adults.

Health risk assessment of organochlorine pesticides with emphasis on DDTs and HCHs in abandoned agricultural soils.

The aim of this study was to evaluate the consequence of changing land use from agriculture land to other use purposes with respect to OCPs non-cancer and cancer risk on human health, based on concentrations of DDTs and HCHs in soils collected from 55 locations representing 12 different land use types. There were no non-cancer risks of DDTs and γ-HCHs on adults and children, and there were very low cancer risks of DDD, o,p'-DDE, DDT, α-HCH, ß-HCH, γ-HCH based on their total concentrations in all samples. Nonetheless, there were significant correlations of DDT to its metabolites (DDE and DDD) (r = 0.506 and r = 0.648) and DDE to DDD (r = 0.438) both at p < 0.01. OCP levels should be routinely monitored in different environmental media and food in order to verify whether there is fresh input. Their potential risks on human health should also be assessed.

Chemical and bioanalytical characterization of dioxins in indoor dust in Hong Kong.

In the present work, air-conditioner filter dust samples collected from commercial office, secondary school, shopping mall, electronic factory and manufacturing plant in Hong Kong were collected for 7-ethoxyresorufin O-deethylase (EROD) assay using a hepatoma cell line (H4IIE) and chemical analysis of dioxins including polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and PCBs with dioxin-like structure. The result of EROD assay showed that bioassay derived TEQ of 2,3,7,8-TCDD (TEQ(bio)) of dust samples varied from 320 to 730 pg/g. Chemical analyses revealed that chemical derived TEQ of 2,3,7,8-TCDD (TEQ(cal)) of dust samples ranged from 134 to 531 pg/g. In addition, the TEQ(cal) of samples were significantly correlated with TEQ(bio) of samples (R=0.83, P<0.01). The average daily doses (ADDs) of dioxins via indoor dust with the estimated ADDs of dioxins via air and food were compared. The results showed that indoor dust is an important medium of exposure to dioxins.

Mutagenicity, genotoxicity and carcinogenic risk assessment of indoor dust from three major cities around the Pearl River Delta.

The mutagenicity and genotoxicity of workplace dust including commercial office, secondary school, shopping mall, hospital, electronics factory and manufacturing plant in Hong Kong and settled house dust from Hong Kong, Shenzhen and Guangzhou were measured. Results indicated that indoor dust contained both frameshift and base pair substitution mutagens. Dust from manufacturing plant showed highest mutagenic potency on TA98±S9 and TA100±S9 activation, whereas, electronics factory showed highest genotoxicity with and without S9 activation. TA100 (-S9) mutagenic potency was significantly correlated with genotoxicity expressed as SOSIP (-S9) of workplace dust (r(2)=0.37, p<0.01). The total PAHs concentration of settled house dust from PRD ranged from 1.63 to 29.2µg/g. Linear regression analyses indicated that the PAHs likely accounted for about 45% of the TA98 with S9 mutagenic activity of workplace dust. TA98 (-S9) mutagenicity (r(2)=0.27, p<0.05) and SOSIP (-S9) of house dust (r(2)=0.41, p<0.01) were both significantly correlated with the number of inhabitants in the house. To achieve a more accurate cancer risk assessment, the oral bioaccessibility of B(a)A, Chry, B(b+k)F, B(a)P, D(ah)A and I(cd)P in different dust ranging from 1.3% to 17% was taken into account. Risk assessments indicated that about 26% of house dust samples resulted in unacceptable cancer risk (>1×10(-6)) for preschool children.

The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust.

Human cytokine array was used to investigate the cytokine profile of U937 and KERTr after exposure to indoor dust or dust extracts. The release of MCP-1 was increased while release of IL-8 and IL-1ß on U937 were decreased after exposure to indoor dust. The releases of RANTES, IL-8 and VEGF from KERTr after exposure to dust extract were increased. The results of IL-8 ELISA assay were consistent with the cytokine array. Real-time RT-PCR was performed to analyze relative changes in gene expression. The MCP-1 mRNA levels were increased after U937 exposure to 18 indoor dust samples, whereas, IL-8 and IL-1ß mRNA level showed both up-regulation and down-regulation. The dose-related increase and decrease response was observed on MCP-1 and IL-8, respectively. Most indoor dust extracts increased RANTES, IL-8 and VEGF mRNA levels on KERTr. The dose-dependent response was observed on RANTES and IL-8. A significant correlation (r=0.48, p<0.05) was obtained between the total PAHs concentration in dust extracts and the induction of RANTES mRNA.

The effect of arbuscular mycorrhizal fungi and phosphate amendement on arsenic uptake, accumulation and growth of Pteris vittata in As-contaminated soil.

This study investigated the contributions of mixed arbuscular mycorrhizal fungi (AMF) inoculum-i.e., mixed populations of indigenous mycorrhiza (Glomus intraradices, Glomus geosporum, Glomus mosseae) (IM) isolated from arsenic (As) contaminated soil and non-indigenous mycorrhiza such as G. mosseae (GM), which possess metal tolerance characteristics-and the addition of phosphate rock (PR) towards the uptake and accumulation of As by Pteris vittata (As hyperaccumlator) grown in As-contaminated soil. Regardless of As levels added to soil, plant growth was substantially improved in amended treatments when compared with the control. In addition, root surface area (0 mg/kg As: 15.2 cm2; 150 mg/kg As: 16.9 cm2; 300 mg/kg As: 20.7 cm2), chlorophyll contents (0 mg/kg As: 1.16 mg/g; 150 mg/kg As: 1.46 mg/g; 300 mg/kg As: 1.81 mg/g) and As translocation factor (0 mg/kg As: 0; 150 mg/kg As: 4.29; 300 mg/kg As: 5.22) in P. vittata of PR+IM/GM were also increased. Such combination could further enhance plant growth (indicated by higher N, P and chlorophyll contents) and As uptake by P. vittata.

Health risk assessment of abandoned agricultural soils based on heavy metal contents in Hong Kong, the world's most populated city.

The objective of this study was to evaluate the consequence of changing and using agricultural soils to other purposes in Hong Kong with respect to risk to human health. This study established concentrations of the following priority elements: As, Cu, Cd, Cr, Pb and Zn in terms of total burden (using mixed acid microwave digestion) and with respect to metal bioaccessibility (using an in vitro simulated gastric solution). 55 locations were sampled representing 12 different land use types, namely, agricultural (A), abandoned agricultural (Ab), organic farm (OF), container storage (CS), construction waste (CW), e-waste storage (EW (S)), e-waste dismantling workshop (EW (DW)), e-waste open burning site (EW (OBS)), open burning site (OBS), petrol station (PS), metal recycling workshop (MRW) and car dismantling workshop (CDW). The elemental concentrations were subsequently used to establish Hazard Indices (for adults and children). 95th percentile values of total elemental concentrations were used to derive a combined (ingestion, dermal and inhalation) Hazard Index (HI) only for adults where the EW (DW) land use type indicated the potential for increased harm (HI=1.16). On the other hand, where 5th percentile values of total elemental concentrations were used to derive a combined Hazard Index (HI) for children the HI values exceeded 1 for CS, MRW, PS, EW (DW), EW (OBS) and CDW land use types (respectively, 1.21, 1.19, 1.52, 1.21, 1.81 and 2.04).

Polycyclic aromatic hydrocarbons (PAHs) in different indoor dusts and their potential cytotoxicity based on two human cell lines.

In this study, 55 air-conditioner filter dust samples from six different workplaces including commercial office, secondary school, shopping mall, hospital, electronic factory and manufacturing plant in Hong Kong were collected for analyses of PAH concentration and cytotoxicity. Chemical analyses showed that the total PAHs ranged from 1.17 to 25.5 microg/g, with the dust samples from manufacturing plant having the highest concentration. MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrasodium bromide) assay was performed to evaluate the cytotoxicity of organic dust extracts using human hepatocellular liver carcinoma cell line (HepG2) and human skin keratinocyte cell line (KERTr). Each organic dust extract showed marked dose-related response. Dust samples from the manufacturing plant showed the highest cytotoxicity. Curve estimation indicated that power model was fit for explaining the relationship between the total PAH concentration and lethal concentration 50 (LC(50)). In addition, a significant negative correlation was observed between the total PAH concentration and LC(50) both on HepG2 (r=-0.65, p<0.01) and KERTr (r=-0.63, p<0.01) cell lines. Source analyses demonstrated that the PAHs in dust were derived from pyrogenic origins.