RESUMO
Chlorogenic acid (3-O-caffeoylquinic acid, CA) is the active component in several botanical beverage, vegetables, fruits, and herbal drugs. The effect of water boiling on the bioactivity of CA was studied. CA could be isomerized to 4-O-caffeoylquinic acid (4-O-CA) and 5-O-caffeoylquinic acid (5-O-CA) in decoctive extraction, and each of the isomers occupied about one-third of the total caffeoylquinic acids. A novel method, using water elution of microsphere resin, was used to purify CA and its 2 isomers. The yield of CA, 4-O-CA, and 5-O-CA was 82%, 5.6%, and 50%, with the purity of 98%, 97%, and 99%, respectively. The DPPH radical scavenging assay showed that 4-O-CA, 5-O-CA, and CA exhibited similar activity. However, there was no significant difference between 4-O-CA and 5-O-CA when used against CCl4-induced toxicity in hepG2 cells. Our studies show that isomerization is the main transformation of CA in boiling, and the decoction could not decrease the anti-oxidant activity of CA.
Assuntos
Ácido Clorogênico/análogos & derivados , Sequestradores de Radicais Livres/farmacologia , Hepatócitos/efeitos dos fármacos , Ácido Quínico/análogos & derivados , Intoxicação por Tetracloreto de Carbono/tratamento farmacológico , Sobrevivência Celular/efeitos dos fármacos , Ácido Clorogênico/química , Ácido Clorogênico/isolamento & purificação , Ácido Clorogênico/farmacologia , Ácido Clorogênico/uso terapêutico , Estabilidade de Medicamentos , Etnofarmacologia , Flores/química , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/isolamento & purificação , Sequestradores de Radicais Livres/uso terapêutico , Células Hep G2 , Temperatura Alta , Humanos , Concentração de Íons de Hidrogênio , Hidrólise , Isomerismo , Lonicera/química , Medicina Tradicional Chinesa , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Ácido Quínico/química , Ácido Quínico/isolamento & purificação , Ácido Quínico/farmacologia , Ácido Quínico/uso terapêuticoRESUMO
Naturally occurring plant alkaloids, in particular those identified from herbal medicines, are finding therapeutic use. Heart diseases can be well managed with specific formulations of herbal medicines. The combined action of multiple constituents of herbal medicines works with therapeutic benefits in humans. The established formulations of Traditional Chinese medicines show efficacy in treatment of diseases. However, individual herbal principles seldom show pharmacological activity. Nevertheless, some of the active alkaloids and terpenoids from medicinal herbs have been identified. The pharmacological activities of these herbal compounds have been studied. These active constituents of herbal medicine are also used in nutrient supplements, but the modes of action of the active component remain sketchy. The present review describes the recent development of those active principles from herbal medicines as cardiovascular agents. The study will provide insights into herbal medicines for drug development for the treatment of cardiovascular disease.