Mercury Stable Isotopes Reveal the Vertical Distribution and Trophic Ecology of Deep-Pelagic Organisms over the North-East Atlantic Ocean Continental Slope.

Deep-pelagic species are central to marine ecosystems and increasingly vulnerable to global change and human exploitation. To date, our understanding of these communities remains limited mainly due to the difficulty of observations, calling for complementary innovative tools to better characterize their ecology. We used mercury (Δ199Hg, δ202Hg, Δ201Hg, and Δ200Hg), carbon (δ13C), and nitrogen (δ15N) stable isotope compositions to segregate deep-pelagic species caught on the continental slope of the Bay of Biscay (NE Atlantic) according to their foraging depth and trophic ecology. Decreasing fish Δ199Hg values with corresponding depth estimates from the surface to down to 1,800 m confirmed that mercury isotopes are able to segregate deep species over a large vertical gradient according to their foraging depth. Results from isotopic compositions also identified different mercury sources, likely reflecting different trophic assemblages over the continental slope, in particular, the demersal influence for some species, compared to purely oceanic species. Overall, our results demonstrate how mercury stable isotopes can inform the vertical foraging habitat of little-known species and communities feeding in the deep.

Trophic guilds and niche segregation among marine megafauna in the Bay of Biscay.

The structure and functioning of ecosystems are largely determined by the interactions between species within a biological community. Among these interactions, species exhibiting similar vertical and spatial prey preferences can be identified, thereby belonging to the same trophic guild. Our study explored some trophic characteristics of a diverse megafaunal community (cetaceans, tunas, seabirds) in the Bay of Biscay (BoB). Using stable isotope analysis (SIA), we explored the dietary habits and niche overlap among predators. The degree of isotopic niche overlap was generally low, but with certain species exhibiting large and narrow isotopic niche areas (long-finned pilot whales and Balearic shearwaters, respectively). Our results revealed a diversity of dietary preferences leading to the identification of three distinct trophic guilds based on prey functional groups and spatial preferences: cephalopod feeders (e.g. long-finned pilot whales, Cuvier's beaked whales, striped dolphins), crustacean feeders (e.g. fin whales, albacores), and piscivores (e.g. common dolphins, harbour porpoises, bottlenose dolphins, Atlantic bluefin tunas, Balearic shearwaters). Our findings showed resource partitioning and niche differentiation among the megafaunal community, highlighting the complexity of BoB's marine ecosystem. The insights derived from this study hold important implications for ecosystem management and the implementation of conservation initiatives.

Ontogenetic shift or not? Different foraging trade-offs within the meso- to bathypelagic fish community.

During ontogeny, the increase in body size forces species to make trade-offs between their food requirements, the conditions necessary for growth and reproduction as well as the avoidance of predators. Ontogenetic changes are leading species to seek out habitats and food resources that meet their needs. To this end, ontogenetic changes in nocturnal habitat (vertical use of the water column) and in the type of food resources (based on stable isotopes of nitrogen) were investigated in 12 species of deep pelagic fish from the Bay of Biscay in the Northeast Atlantic. Our results revealed the existence of major differences in the ontogenetic strategies employed by deep pelagic fishes. Some species showed ontogenetic changes in both vertical habitat use and food resources (e.g. Jewel lanternfish (Lampanyctus crocodilus) and Atlantic soft pout (Melanostigma atlanticum)). In contrast, other species showed no ontogenetic change (e.g. Koefoed's searsid (Searsia koefoedi) and Lancet fish (Notoscopelus kroyeri)). Some species only changed food resources (e.g. Spotted lanternfish (Myctophum punctatum), Spotted barracudina (Arctozenus risso) and Stout sawpalate (Serrivomer beanii)), while others seemed to be influenced more by depth than by trophic features (e.g. Bluntsnout smooth-head (Xenodermichthys copei) and Olfer's Hatchetfish (Argyropelecus olfersii)). These results suggest that to meet their increasing energy requirements during ontogeny, some species have adopted a strategy of shifting their food resources (larger prey or prey with a higher trophic level), while others seemed to maintain their food resources but are most likely increasing the quantity of prey ingested. As fish species can have different functional roles during their development within ecosystems, characterising ontogenetic changes in mesopelagic fish species is a crucial step to be considered in future research aimed at understanding and modelling the complexity of deep-pelagic food webs.

Composition of cetacean communities worldwide shapes their contribution to ocean nutrient cycling.

Defecation by large whales is known to fertilise oceans with nutrients, stimulating phytoplankton and ecosystem productivity. However, our current understanding of these processes is limited to a few species, nutrients and ecosystems. Here, we investigate the role of cetacean communities in the worldwide biological cycling of two major nutrients and six trace nutrients. We show that cetaceans release more nutrients in mesotrophic to eutrophic temperate waters than in oligotrophic tropical waters, mirroring patterns of ecosystem productivity. The released nutrient cocktails also vary geographically, driven by the composition of cetacean communities. The roles of small cetaceans, deep diving cetaceans and baleen whales differ quantitatively and functionally, with contributions of small cetaceans and deep divers exceeding those of large whales in some areas. The functional diversity of cetacean communities expands beyond their role as top predators to include their role as active nutrient vectors, which might be equally important to local ecosystem dynamics.

Variation of 210-polonium in the cephalopod community from the Bay of Biscay, North-East Atlantic.

Among natural radionuclides, 210Po is the major contributor to the radiation dose received by marine organisms. In cephalopods, 210Po is concentrated in the digestive gland, which contains over 90% of the whole-body burden of the nuclide. Although previous studies showed that 210Po was taken up independently of 210Pb, its parent nuclide, very little is known about the factors influencing its levels in cephalopods. To the best of our knowledge, no studies investigated 210Po levels in different species at the same time. In the present study, 210Po was analysed in the digestive gland of 62 individuals from 11 species representing a large range of feeding ecologies and habitats, including squids, cuttlefish and octopus species from coastal to deep-oceanic habitats. Among species, the highest activity was measured in Loligo vulgaris (5720 ± 3606 Bq/kg) and the lowest in T. megalops (188 Bq/kg). However, considering the habitats (benthic vs pelagic and neritic vs oceanic), no significant differences appeared. At the species level, no differences between sexes were found so both sexes were plotted together to test the size effect for species with at least 8 individuals (i.e., Eledone cirrhosa, L. vulgaris, L. forbesi and Sepia officinalis). In the first three species, 210Po levels decreased significantly with increasing size or weight but not in S. officinalis. In squid, this could be related to ontogenetic changes in diet from a high proportion of crustaceans (high Po content) in small individuals to fish (low Po content) in larger individuals, while the high dietary plasticity of S. officinalis at all stages of its life cycle could explain the lack of decrease in 210Po with size. In comparison to the few data from the literature, the levels of 210Po concentrations in the cephalopod community of the Bay of Biscay were overall in the same range than those reported in other cephalopods, varying across 4 orders of magnitude. Further studies are needed to understand the mechanism of retention in the cephalopod digestive gland.

Development of an integrated indicator to assess chemical contamination in different marine species: The case of mercury on the French Atlantic continental shelf.

Good Environmental Status (GES) for Descriptor 8 (D8) of the Marine Strategy Framework Directive (MSFD) is considered to be achieved when concentrations of contaminants are at levels not giving rise to pollution effects. This study proposes a framework to assess GES in marine waters adjacent to France, including four groups of species (bivalves, fish, birds and mammals) living on the continental shelf and covering different dimensions of the marine environment. This framework is applied to mercury (Hg) in the three marine regions along the French Atlantic coast and includes two assessment types: i) an absolute assessment by comparing contamination levels with environmental thresholds, and ii) a relative assessment by comparing contamination levels over time, performed for bivalves and mammals that had long time-series available. Mercury concentrations were higher than environmental thresholds for bivalves and fish in all the three studied regions. Plus, they significantly increased since the 2000s for most bivalve stations and for the common dolphin Delphinus delphis. Our results therefore indicate that Hg concentrations have increased in marine waters and have reached levels possibly giving rise to pollution effects in biota from the three marine regions. The present study also highlighted the complementarity of monitoring Hg concentrations in each group of species and each type of assessment, making it possible to propose a conceptual framework for assessing the environmental pressure of bioaccumulated and biomagnified contaminants over the continental shelf.

Trace elements, dioxins and PCBs in different fish species and marine regions: Importance of the taxon and regional features.

Chemical contaminant concentrations in wild organisms are used to assess environmental status under the European Marine Strategy Framework Directive. However, this approach is challenged by the complex intra- and inter-species variability, and the different regional features. In this study, concentrations in trace elements (As, Cd, Hg and Pb), polychlorinated biphenyls (PCBs), polychlorodibenzo-para-dioxines (PCDDs) and polychlorodibenzofuranes (PCDFs) were monitored in 8 fish species sampled on the continental shelf of three French regions: the Eastern English Channel (EEC) and Bay of Biscay (BoB) in the Northeast Atlantic Ocean, and the Gulf of Lions (GoL) in Western Mediterranean Sea. Our objectives were to identify species or regions more likely to be contaminated and to assess how to take this variability into account in environmental assessment. While concentrations were higher in benthic and demersal piscivores, PCB and PCDD/F concentrations (lipid-weight) were similar in most teleost species. For Cd, Hg and Pb, the trophic group accumulating the highest concentrations depended on the contaminant and region. Concentrations in Hg, PCBs and PCDD/Fs were higher in the EEC and/or GoL than in BoB. Cadmium and Pb concentrations were highest in the BoB. Lipid content accounted for 35%-84% of organic contaminant variability. Lipid normalisation was employed to enhance robustness in the identification of spatial patterns. Contaminant patterns in chondrichthyans clearly differed from that in teleosts. In addition, trophic levels accounted for ≤1% and ≤33% of the contaminant variability in teleost fishes in the EEC and BoB, respectively. Therefore, developing taxa-specific thresholds might be a more practical way forward for environmental assessment than normalisation to trophic levels.

MesopTroph, a database of trophic parameters to study interactions in mesopelagic food webs.

Mesopelagic organisms play a crucial role in marine food webs, channelling energy across the predator-prey network and connecting depth strata through their diel vertical migrations. The information available to assess mesopelagic feeding interactions and energy transfer has increased substantially in recent years, owing to the growing interest and research activity in the mesopelagic realm. However, such data have not been systematically collated and are difficult to access, hampering estimation of the contribution of mesopelagic organisms to marine ecosystems. Here we present MesopTroph, a georeferenced database of diet, trophic markers, and energy content of mesopelagic and other marine taxa compiled from 203 published and non-published sources. MesopTroph currently includes data on stomach contents, carbon and nitrogen stable isotopes, major and trace elements, energy density, fatty acids, trophic positions, and diet proportion estimates for 498 species/genera. MesopTroph will be expanded with new data emerging from ongoing studies. MesopTroph provides a unique tool to investigate trophic interactions and energy flow mediated by mesopelagic organisms, and to evaluate the ecosystem services of this community.

Trace metal elements and organic contaminants are differently related to the growth and body condition of wild European sea bass juveniles.

Chemical contaminants are one of the causes of the ongoing degradation of coastal and estuarine nurseries, key functional habitats in which the juveniles of many marine species grow. As chemical contaminants can cause a decrease in the energy available and induce defence mechanisms reducing the amount of energy allocated to life history traits, quantifying their effect on the fitness of juvenile fish is key to understand their population-level consequences. However, these effects are primarily estimated experimentally or in the wild but on a limited number of contaminants or congeners that do not reflect the wide variety of chemical contaminants to which juvenile fish are exposed. To address this issue, we measured concentrations of 14 trace metal elements (TMEs) and bioaccumulative organic contaminants (OCs) in European sea bass juveniles (1-year-old) from three major French nurseries (Seine, Loire and Gironde estuaries). We tested the hypotheses that (i) levels and profiles of contaminants differed among studied nurseries, and ii) fish growth and body condition (based on morphometric measurements and muscle C:N ratio) were lower in individuals with higher contaminant concentrations. Multivariate analyses showed that each nursery had distinct contaminant profiles for both TMEs and OCs, confirming the specific contamination of each estuary, and the large array of contaminants accumulated by sea bass juveniles. Increasing concentrations in some TMEs were associated to decreased growth, and TMEs were consistently related to lower fish body condition. The effect of OCs was more difficult to pinpoint possibly due to operational constraints (i.e., analyses on pooled fish) with contrasting results (i.e., higher growth and decreased body condition). Overall, this study shows that chemical contaminants are related to lower fish growth and body condition at an early life stage in the wild, an effect that can have major consequences if sustained in subsequent ages and associated with a decline in survival and/or reproductive success.

Two cetacean species reveal different long-term trends for toxic trace elements in European Atlantic French waters.

Cetaceans have been naturally exposed to toxic trace elements (TEs) on an evolutionary time scale. Hence, they have developed mechanisms to control and/or mitigate their toxic effects. These long-lived species located at high trophic positions and bioaccumulating toxic elements are assumed to be good biomonitoring organisms. However, anthropogenic emissions have strongly increased environmental levels of toxic TEs in the last decades, questioning the efficiency of the detoxication mechanisms in cetaceans. In this context, temporal trends of mercury (Hg), cadmium (Cd) and lead (Pb) concentrations were studied through the analysis of 264 individuals from two cetacean species the common dolphin (Delphinus delphis) and the harbour porpoise (Phocoena phocoena) and belonging to two different Management Units (MUs) for the latter. These individuals stranded along the French Atlantic coasts from 2000s to 2017. All the trends presented were age- and sex-corrected and stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) were measured as proxies of their feeding ecology. Results showed that Pb concentrations clearly decreased over time in both species and MUs. This decrease agrees with the lead petrol regulation after 2000s, supporting the use of these species as valuable bioindicators of changes for TE levels in the marine environment. A significant long-term increase of total Hg concentrations was only observed in common dolphins. Cadmium concentrations also revealed different trends over the period in both species. The different Hg and Cd trends observed in the two species, probably reflected a contrasted contamination of habitat and prey species than a global increase of the contamination in the environment. These results highlight the necessity and gain of using different species to monitor changes in marine environments, each of them informing on the contamination of its own ecological niche. Lastly, the Se:Hg molar ratios of species suggested a low risk for Hg toxicity over time.

High inter-species variability in elemental composition of the twilight zone fauna varies implications for predators and exploitation by humans.

While the importance of oceanic micronektonic species in biogeochemical cycles and in the transfer of matter in food webs is globally recognized, specific knowledge on elemental concentrations and their variability within this community is still poorly documented. Here, we report for the first time in the Bay of Biscay, North-East Atlantic, the body composition in various biological parameters and chemical elements of a meso-to bathypelagic micronektonic community. Stable carbon and nitrogen isotope compositions (δ13C, δ15N), C:N ratios, energy density, as well as the concentrations in 6 macro-minerals and 13 trace elements including essential (micro-nutrients) and non-essential elements (undesirables, with no know biological function) were measured in whole organisms of 4 crustacean and 11 fish species caught simultaneously around 800 m depth. The results showed a low variability of δ13C values, confirming that all studied species share the same habitat. On the contrary, large differences were observed among species for several elements. Trace elements showed the greatest variability (i.e. larger range of values), especially silver (Ag), arsenic (As), cadmium (Cd), cobalt and vanadium. Significant differences were also revealed among taxa for Ag, As, Cd, copper and strontium concentrations (with crustaceans > fish), as well as for δ15N values and phosphorus concentrations (with fish > crustaceans). Although concentrations varied greatly among species, they could be grouped according to their energy density and composition in 19 chemical elements, through hierarchical clustering analysis. Six functional groups of species have been thus identified, reflecting contrasted nutritional benefit and/or exposure to undesirables for predators feeding on this deep pelagic community. Finally, the concentrations measured for the potentially toxic trace elements (undesirables) exceeded the existing European thresholds for Cd and to a lesser extent mercury (Hg), which point out potential risks in the perspective of a future exploitation of these deep living resources by humans.

Differences in chemical contaminants bioaccumulation and ecotoxicology biomarkers in Mytilus edulis and Mytilus galloprovincialis and their hybrids.

The Mytilus mussels are spread all over the world and many related species coexist in several areas and can produce hybrid offspring. Mussels have been used for decades in national and international programs to monitor chemical contamination in the environment. Differences in bioaccumulation and biotransformation abilities between species and their hybrids should be evaluated to assess the comparability of the results obtained within the international biomonitoring programs. The objective of this study was to characterize bioaccumulation abilities and biomarker responses in Mytilus edulis, Mytilus galloprovincialis and their hybrids via an in situ transplantation experimentation on their progenies. Four mussel groups (M. edulis, M. galloprovincialis and two hybrids batches) issued from genetically characterized parents were transplanted for one year in Charente Maritime (France) to ensure their exposure to identical sources of contamination. The bioaccumulation of several families of contaminants (trace metals, polycyclic aromatic hydrocarbons, polybrominated diphenyl ethers, polychlorinated biphenyls), the response of several biomarkers (DNA strand breaks level, lysosomal membrane stability, metallothionein content, acetylcholine esterase activity) and some physiological parameters (growth, mortality, gonadal development), were analyzed. Differences were observed between species, however they were contaminant-specific. Variations in contaminants levels were observed between progenies, with higher levels of Cu, PBDE, PCB in M. edulis, and higher levels of Cd, Hg, Zn in M galloprovincialis. This study demonstrated that variations in contaminant bioaccumulation and different biomarker responses exist between Mytilus species in the field. Data on species or the presence of hybrid individuals (or introgression) is an important additional parameter to add to biomonitoring programs databases.

Metal stable isotopes in transplanted oysters as a new tool for monitoring anthropogenic metal bioaccumulation in marine environments: The case for copper.

Metal release into the environment from anthropogenic activities may endanger ecosystems and human health. However, identifying and quantifying anthropogenic metal bioaccumulation in organisms remain a challenging task. In this work, we assess Cu isotopes in Pacific oysters (C. gigas) as a new tool for monitoring anthropogenic Cu bioaccumulation into marine environments. Arcachon Bay was taken as a natural laboratory due to its increasing contamination by Cu, and its relevance as a prominent shellfish production area. Here, we transplanted 18-month old oysters reared in an oceanic neighbor area into two Arcachon Bay mariculture sites under different exposure levels to continental Cu inputs. At the end of their 12-month long transplantation period, the oysters' Cu body burdens had increased, and was shifted toward more positive δ65Cu values. The gradient of Cu isotope compositions observed for oysters sampling stations was consistent with relative geographic distance and exposure intensities to unknown continental Cu sources. A binary isotope mixing model based on experimental data allowed to estimate the Cu continental fraction bioaccumulated in the transplanted oysters. The positive δ65Cu values and high bioaccumulated levels of Cu in transplanted oysters support that continental emissions are dominantly anthropogenic. However, identifying specific pollutant coastal source remained unelucidated mostly due to their broader and overlapping isotope signatures and potential post-depositional Cu isotope fractionation processes. Further investigations on isotope fractionation of Cu-based compounds in an aqueous medium may improve Cu source discrimination. Thus, using Cu as an example, this work combines for the first time a well-known caged bivalve approach with metal stable isotope techniques for monitoring and quantifying the bioaccumulation of anthropogenic metal into marine environments. Also, it states the main challenges to pinpoint specific coastal anthropogenic sources utilizing this approach and provides the perspectives for further studies to overcome them.

Patterns of trace metal bioaccumulation and trophic transfer in a phytoplankton-zooplankton-small pelagic fish marine food web.

Trace metal contamination in the European sardine and anchovy food web was investigated in the Gulf of Lions, NW Mediterranean Sea, including seawater and size fractions of plankton. The results highlighted: i) higher and more variable concentrations in the smaller plankton size classes for all metals except cadmium; ii) higher concentrations in anchovy versus sardine for all elements except lead; iii) different patterns of metal bioaccumulation through the food web: cobalt, nickel, copper, silver, lead and zinc displayed continuously decreasing concentrations (with the exception of increased zinc in fish only), while mercury concentrations dropped considerably in larger plankton size classes and rose significantly in fish. Lastly, cadmium concentrations were found to be highest in intermediate plankton size classes, with very low levels in fish. The need to efficiently characterize the biological composition of plankton in order to fully identify its role in the mobilization and transfer of metals was highlighted.

Trace metal concentrations in the muscle of seven marine species: Comparison between the Gulf of Lions (North-West Mediterranean Sea) and the Bay of Biscay (North-East Atlantic Ocean).

Concentrations of 6 trace metals (Ag, Cd, Cu, Ni, Pb, Zn) in the muscle of 2 sharks (Galeus melastomus and Scyliorhinus canicula), 4 teleosts (Helicolenus dactylopterus, Lepidorhombus boscii, Micromesistius poutassou and Phycis blennoides) and 1 crustacean (Nephrops norvegicus) were compared between the Bay of Biscay (Atlantic Ocean) and the Gulf of Lions (Mediterranean Sea). Although average concentrations and the trace element pollution index were generally higher in the Gulf of Lions, significant differences between the two ecosystems were only found for Zn for Helicolenus dactylopterus, and for Ag and Cu for the crustacean N. norvegicus. Moreover, some relationships between trophic level or size and metal concentrations were found for these two species. The absence of clear pattern may result from the blurring effect of contamination and excretion that may act differentially for all species and all elements.

A global perspective on the trophic geography of sharks.

Sharks are a diverse group of mobile predators that forage across varied spatial scales and have the potential to influence food web dynamics. The ecological consequences of recent declines in shark biomass may extend across broader geographic ranges if shark taxa display common behavioural traits. By tracking the original site of photosynthetic fixation of carbon atoms that were ultimately assimilated into muscle tissues of 5,394 sharks from 114 species, we identify globally consistent biogeographic traits in trophic interactions between sharks found in different habitats. We show that populations of shelf-dwelling sharks derive a substantial proportion of their carbon from regional pelagic sources, but contain individuals that forage within additional isotopically diverse local food webs, such as those supported by terrestrial plant sources, benthic production and macrophytes. In contrast, oceanic sharks seem to use carbon derived from between 30° and 50° of latitude. Global-scale compilations of stable isotope data combined with biogeochemical modelling generate hypotheses regarding animal behaviours that can be tested with other methodological approaches.

Carbon and nitrogen elemental and isotopic ratios of filter-feeding bivalves along the French coasts: An assessment of specific, geographic, seasonal and multi-decadal variations.

Primary consumers play a key role in coastal ecosystems by transferring organic matter from primary producers to predators. Among them, suspension-feeders, like bivalve molluscs are widely used in trophic web studies. The main goal of this study was to investigate variations of C and N elemental and isotopic ratios in common bivalves (M. edulis, M. galloprovincialis, and C. gigas) at large spatial (i.e. among three coastal regions) and different temporal (i.e. from seasonal to multi-decadal) scales in France, in order to identify potential general or specific patterns and speculate on their drivers. The observed spatial variability was related to the trophic status of the coastal regions (oligotrophic Mediterranean Sea versus meso- to eutrophic English Channel and Atlantic ocean), but not to ecosystem typology (estuaries, versus lagoons versus bays versus littoral systems). Furthermore, it highlighted local specificities in terms of the origin of the POM assimilated by bivalves (e.g., mainly continental POM vs. marine phytoplankton vs. microphytobenthic algae). Likewise, seasonal variability was related both to the reproduction cycle for C/N ratios of Mytilus spp. and to changes in trophic resources for δ13C of species located close to river mouth. Multi-decadal evolution exhibited shifts and trends for part of the 30-year series with decreases in δ13C and δ15N. Specifically, shifts appeared in the early 2000's, likely linking bivalve isotopic ratios to a cascade of processes affected by local drivers.

Oligotrophy as a major driver of mercury bioaccumulation in medium-to high-trophic level consumers: A marine ecosystem-comparative study.

Mercury (Hg) is a global contaminant of environmental concern. Numerous factors influencing its bioaccumulation in marine organisms have already been described at both individual and species levels (e.g., size or age, habitat, trophic level). However, few studies have compared the trophic characteristics of ecosystems to explain underlying mechanisms of differences in Hg bioaccumulation and biomagnification among food webs and systems. The present study aimed at investigating the potential primary role of the trophic status of systems on Hg bioaccumulation and biomagnification in temperate marine food webs, as shown by their medium-to high-trophic level consumers. It used data from samples collected at the shelf-edge (i.e. offshore organisms) in two contrasted ecosystems: the Bay of Biscay in the North-East Atlantic Ocean and the Gulf of Lion in the North-West Mediterranean Sea. Seven species including crustaceans, sharks and teleost fish, previously analysed for their total mercury (T-Hg) concentrations and their stable carbon and nitrogen isotope compositions, were considered for a meta-analysis. In addition, methylated mercury forms (or methyl-mercury, Me-Hg) were analysed. Mediterranean organisms presented systematically lower sizes than Atlantic ones, and lower δ13C and δ15N values, the latter values especially highlighting the more oligotrophic character of Mediterranean waters. Mediterranean individuals also showed significantly higher T-Hg and Me-Hg concentrations. Conversely, Me-Hg/T-Hg ratios were higher than 85% for all species, and quite similar between systems. Finally, the biomagnification power of Hg was different between systems when considering T-Hg, but not when considering Me-Hg, and was not different between the Hg forms within a given system. Overall, the different parameters showed the crucial role of the low primary productivity and its effects rippling through the compared ecosystems in the higher Hg bioaccumulation seen in organisms from oligotrophic Mediterranean waters.

Chemical contaminants (trace metals, persistent organic pollutants) in albacore tuna from western Indian and south-eastern Atlantic Oceans: Trophic influence and potential as tracers of populations.

Albacore tuna (Thunnus alalunga) is a highly commercial fish species harvested in the world's Oceans. Identifying the potential links between populations is one of the key tools that can improve the current management across fisheries areas. In addition to characterising populations' contamination state, chemical compounds can help refine foraging areas, individual flows and populations' structure, especially when combined with other intrinsic biogeochemical (trophic) markers such as carbon and nitrogen stable isotopes. This study investigated the bioaccumulation of seven selected trace metals - chromium, nickel, copper (Cu), zinc (Zn), cadmium (Cd), mercury (Hg) and lead - in the muscle of 443 albacore tunas, collected over two seasons and/or years in the western Indian Ocean (WIO: Reunion Island and Seychelles) and in the south-eastern Atlantic Ocean (SEAO: South Africa). The main factor that explained metal concentration variability was the geographic origin of fish, rather than the size and the sex of individuals, or the season/year of sampling. The elements Cu, Zn, Cd and Hg indicated a segregation of the geographic groups most clearly. For similar sized-individuals, tunas from SEAO had significantly higher concentrations in Cu, Zn and Cd, but lower Hg concentrations than those from WIO. Information inferred from the analysis of trophic markers (δ13C, δ15N) and selected persistent organic pollutants, as well as information on stomach contents, corroborated the geographical differences obtained by trace metals. It also highlighted the influence of trophic ecology on metal bioaccumulation. Finally, this study evidenced the potential of metals and chemical contaminants in general as tracers, by segregating groups of individuals using different food webs or habitats, to better understand spatial connectivity at the population scale. Limited flows of individuals between the SEAO and the WIO are suggested. Albacore as predatory fish also provided some information on environmental and food web chemical contamination in the different study areas.

An assessment of contaminant concentrations in toothed whale species of the NW Iberian Peninsula: part I. Persistent organic pollutants.

Concentrations and patterns of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in the blubber of the five most common toothed whales off the Northwest Iberian Peninsula (NWIP), specifically common dolphin, long-finned pilot whale, harbour porpoise, striped dolphin and bottlenose dolphin, were investigated. The study revealed that differences in PCB and PBDE concentrations among the species are highly dependent on age and sex but also on ecological factors such as trophic level, prey type and habitat. Of the five species studied, bottlenose dolphin and harbour porpoise showed the greatest concentrations of PCBs. Both species exceed the toxic threshold of 17µgg(-1) lipid weight (PCB Aroclor equivalent) for health effects on marine mammals, for 100% and 75% of the individuals tested, respectively. Overall, the PCB and PBDE levels observed in the NWIP toothed whales were of the same order of magnitude or lower than those reported by previous studies in areas of the NE Atlantic. However, they are often higher than those for toothed whales from the southern Atlantic and Pacific Ocean.