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2.
BMC Biol ; 15(1): 63, 2017 07 31.
Artigo em Inglês | MEDLINE | ID: mdl-28756777

RESUMO

BACKGROUND: Helicoverpa armigera and Helicoverpa zea are major caterpillar pests of Old and New World agriculture, respectively. Both, particularly H. armigera, are extremely polyphagous, and H. armigera has developed resistance to many insecticides. Here we use comparative genomics, transcriptomics and resequencing to elucidate the genetic basis for their properties as pests. RESULTS: We find that, prior to their divergence about 1.5 Mya, the H. armigera/H. zea lineage had accumulated up to more than 100 more members of specific detoxification and digestion gene families and more than 100 extra gustatory receptor genes, compared to other lepidopterans with narrower host ranges. The two genomes remain very similar in gene content and order, but H. armigera is more polymorphic overall, and H. zea has lost several detoxification genes, as well as about 50 gustatory receptor genes. It also lacks certain genes and alleles conferring insecticide resistance found in H. armigera. Non-synonymous sites in the expanded gene families above are rapidly diverging, both between paralogues and between orthologues in the two species. Whole genome transcriptomic analyses of H. armigera larvae show widely divergent responses to different host plants, including responses among many of the duplicated detoxification and digestion genes. CONCLUSIONS: The extreme polyphagy of the two heliothines is associated with extensive amplification and neofunctionalisation of genes involved in host finding and use, coupled with versatile transcriptional responses on different hosts. H. armigera's invasion of the Americas in recent years means that hybridisation could generate populations that are both locally adapted and insecticide resistant.


Assuntos
Genoma de Inseto , Herbivoria , Mariposas/genética , Animais , Perfilação da Expressão Gênica , Genômica , Espécies Introduzidas , Larva/genética , Larva/crescimento & desenvolvimento , Mariposas/classificação , Mariposas/crescimento & desenvolvimento , Análise de Sequência de DNA
3.
Plant Sci ; 188-189: 97-101, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22525249

RESUMO

The location of the phloem within a plant, and its vulnerability to disruption, make it a difficult tissue to study and therefore non-invasive studies of phloem functionality are important. Here we compare, phloem transport, measured non-invasively, in wild type Arabidopsis thaliana, and transposon-insertion mutants for AtSUC1 or AtSUC2, giving in vivo information on the importance of these sucrose transporters for phloem transport. The suc2 mutant showed an increase in both phloem leakage and transport time, consistent with reduced sucrose uptake into both transport and collection phloem. The results are consistent with the AtSUC2 transporter being important for retrieval of leaked sucrose in the transport phloem of Arabidopsis. There was no difference in phloem transport properties between the wild type and the suc1 mutants, implying that the AtSUC1 transporter does not play a significant role within the transport phloem of Arabidopsis under the conditions of our study.


Assuntos
Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Proteínas de Membrana Transportadoras/metabolismo , Floema/metabolismo , Proteínas de Plantas/metabolismo , Sacarose/metabolismo , Arabidopsis/genética , Transporte Biológico , Carbono/metabolismo , Radioisótopos de Carbono , Técnicas de Inativação de Genes , Proteínas de Membrana Transportadoras/genética , Mutagênese Insercional , Proteínas de Plantas/genética
4.
Insect Mol Biol ; 19(1): 9-25, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20002216

RESUMO

We have identified lipase-like genes from an Epiphyas postvittana larval midgut EST library. Of the 10 pancreatic lipase family genes, six appear to encode active lipases and four encode inactive lipases, based on the presence/absence of essential catalytic residues. The four gastric lipase family genes appear to encode active proteins. Phylogenetic analysis of 54 lepidopteran pancreatic lipase proteins resolved the clade into five groups of midgut origin and a sixth of non-midgut lipases. The inactive proteins formed two separate groups with highly conserved mutations. The lepidopteran midgut lipases formed a ninth subfamily of pancreatic lipases. Eighteen insect and human gastric lipases were analysed phylogenetically with only very weak support for any groupings. Gene expression was measured in the larval midgut following feeding on five artificial diets and on apple leaves. The artificial diets contained different levels of triacylglycerol, linoleic acid and cholesterol. Significant changes in gene expression (more than 100-fold for active pancreatic lipases) were observed. All the inactive lipases were also highly expressed. The gastric lipase genes were expressed at lower levels and suppressed in larvae feeding on leaves. Together, protein motif analysis and the gene expression data suggest that, in phytophagous lepidopteran larvae, the pancreatic lipases may function in vivo as galactolipases and phospholipases whereas the gastric lipases may function as triacylglycerol hydrolases.


Assuntos
Dieta , Proteínas de Insetos/metabolismo , Lipase/metabolismo , Mariposas/enzimologia , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Digestão , Ensaios Enzimáticos , Feminino , Trato Gastrointestinal/enzimologia , Expressão Gênica , Genes de Insetos , Humanos , Proteínas de Insetos/genética , Larva/enzimologia , Lipase/genética , Masculino , Dados de Sequência Molecular , Mariposas/genética , Filogenia , Alinhamento de Sequência
5.
Insect Mol Biol ; 18(5): 635-48, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19754741

RESUMO

Host cell and virus gene expression were measured five days after per os inoculation of 3rd instar lightbrown apple moth (LBAM) larvae with the Epiphyas postvittana nucleopolyhedrovirus (EppoNPV). Microarray analysis identified 84 insect genes that were up-regulated and 18 genes that were down-regulated in virus-infected larvae compared with uninfected larvae. From the 134 viral open reading frames represented on the microarray, 81 genes showed strong expression. Of the 38 functionally identifiable regulated insect genes, 23 coded for proteins that have roles in one of five processes; regulation of transcription and translation, induction of apoptosis, and maintenance of both juvenility and actin cytoskeletal integrity. Of the 34 functionally identifiable viral genes that were most strongly expressed, 12 had functions associated with these five processes, as did a further seven viral genes which were expressed at slightly lower levels. A survey of the LBAM-expressed sequence tag library identified further genes involved in these processes. In total, 135 insect genes and 38 viral genes were analysed by quantitative polymerase chain reaction. Twenty-one insect genes were strongly up-regulated and 31 genes strongly down-regulated. All 38 viral genes examined were highly expressed. These data suggest that induction of apoptosis and regulation of juvenility are the major 'battlegrounds' between virus and insect, with the majority of changes observed representing viral control of insect gene expression. Transcription and translational effects seem to be exerted largely through modulation of mRNA and protein degradation. Examples of attempts by the insect to repel the infection via changes in gene expression within these same processes were, however, also noted. The data also showed the extent to which viral transcription dominated in the infected insects at five days post inoculation.


Assuntos
Regulação da Expressão Gênica , Malus/parasitologia , Mariposas/genética , Mariposas/virologia , Nucleopoliedrovírus/fisiologia , Animais , Apoptose/genética , Citoesqueleto/genética , Regulação Viral da Expressão Gênica , Genes Virais , Hormônios de Inseto/genética , Larva/genética , Larva/virologia , Nucleopoliedrovírus/genética , Análise de Sequência com Séries de Oligonucleotídeos , Biossíntese de Proteínas/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transcrição Gênica
6.
J Invertebr Pathol ; 101(3): 215-21, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19465026

RESUMO

Amber disease of the New Zealand grass grub Costelytra zealandica (Coleoptera: Scarabaeidae) is caused by ingestion of pADAP plasmid carrying isolates of Serratia entomophila or Serratia proteamaculans (Enterobacteriaceae) and causes infected larvae to cease feeding and clear their midgut to a pale amber colour where midgut serine protease activities are virtually eliminated. Using bacterial strains and mutants expressing combinations of the anti-feeding (afp) and gut clearance (sep) gene clusters from pADAP, we manipulated the disease phenotype and demonstrated directly the relationship between gene clusters, phenotype and loss of enzyme activity. Treatment with afp-expressing strains caused cessation of feeding without gut clearance where midgut protease activity was maintained at levels similar to that of healthy larvae. Treatment with strains expressing sep-genes caused gut clearance followed by a virtual elimination of trypsin and chymotrypsin titre in the midgut indicating both the loss of pre-existing enzyme from the lumen and a failure to replenish enzyme levels in this region by secretion from the epithelium. Monitoring of enzymatic activity through the alimentary tract during expression of disease showed that loss of serine protease activity in the midgut was matched by a surge of protease activity in the hindgut and frass pellets, indicating a flushing and elimination of the midgut contents. The blocking of enzyme secretion through amber disease appears to be selective as leucine aminopeptidase and alpha-amylase were still detected in the midgut of diseased larvae.


Assuntos
Besouros/enzimologia , Proteínas de Insetos/metabolismo , Serratia/fisiologia , Animais , Besouros/microbiologia , Cinética , Larva/enzimologia , Larva/microbiologia , Família Multigênica/fisiologia , Nova Zelândia , Peptídeo Hidrolases/metabolismo , Fenótipo , alfa-Amilases/metabolismo
7.
Insect Mol Biol ; 17(4): 375-85, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18651919

RESUMO

Rapid elimination of midgut luminal proteinase activity and gut clearance are the two major symptoms of amber disease in Costelytra zealandica larvae because of the three-subunit protein toxin complex produced in Serratia entomophila and Serratia proteamaculans. Quantitative PCR analysis of mRNA from the major serine proteinase gene families showed that loss of proteinase activity did not result from transcriptional downregulation. Unexpectedly, protein levels and rates of protein synthesis increased, rather than decreased, in the midgut of diseased insects. Proteomic analysis of midgut tissues showed marked differences between healthy and diseased midguts. Large increases in soluble forms of both actin and tubulin were identified from 2D-gels, together with concurrent decreases in the levels of polymeric actin-associated proteins: actin depolymerizing factor and cyclophilin. These results suggest that the Serratia toxin acts to cause degradation of the cytoskeletal network and prevent secretion of midgut gut digestive proteinases as both the actin cytoskeleton and microtubules are involved in exocytosis. Proteinases synthesized in the diseased midgut must be rapidly degraded because they do not accumulate in an inactive form.


Assuntos
Besouros/microbiologia , Besouros/fisiologia , Exocitose/fisiologia , Serratia/isolamento & purificação , Animais , Proteínas do Citoesqueleto/metabolismo , Trato Gastrointestinal/enzimologia , Trato Gastrointestinal/microbiologia , Larva/microbiologia , Controle Biológico de Vetores , Serina Endopeptidases/metabolismo , Fatores de Tempo
8.
Insect Mol Biol ; 17(3): 247-59, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18477240

RESUMO

Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larval midgut tissue was constructed and analysed to determine the composition and regulation of proteases in the C. zealandica larval midgut. Gene mining identified three trypsin-like and 11 chymotrypsin-like SPs spread among four major subgroups. Representative SPs were examined by quantitative PCR and enzyme activity assayed across developmental stages. The serine protease genes examined were expressed throughout feeding stages and downregulated in nonfeeding stages. The study will improve targeting of protease inhibitors and bacterial disruptors of SP synthesis.


Assuntos
Besouros/enzimologia , Besouros/crescimento & desenvolvimento , Etiquetas de Sequências Expressas , Trato Gastrointestinal/enzimologia , Regulação da Expressão Gênica no Desenvolvimento , Biblioteca Gênica , Serina Endopeptidases/genética , Regiões 3' não Traduzidas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Besouros/genética , Larva/enzimologia , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Serina Endopeptidases/química , Serina Endopeptidases/metabolismo
9.
J Insect Physiol ; 54(2): 518-28, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18199450

RESUMO

Tri-trophic impacts on adult predatory carabid beetles, Ctenognathus novaezelandiae, of insect-resistant transgenic tobacco plants expressing a serine protease inhibitor, bovine spleen trypsin inhibitor (BSTI), or a biotin-binding protein, avidin, were investigated. Both proteins could potentially affect this beetle, since avidin is known to be insecticidal to many beetle species and C. novaezelandiae midguts were shown to contain high levels of trypsin, a protease powerfully inhibited by bovine pancreatic trypsin inhibitor (a BSTI homologue) in vitro. Newly emerged field-collected adult C. novaezelandiae were fed exclusively for 280 days on Spodoptera litura larvae raised either on non-transgenic control, transgenic avidin (55 ppm) or transgenic BSTI (68 ppm) tobacco. Despite this long-term exclusive diet, there was no treatment effect on survival or fecundity and only minor and transient effects on beetles were observed. Data pooled across time and genders showed control-prey-fed beetles weighed 3% more than BSTI-prey-fed beetles and avidin-prey-fed beetles consumed 3-4% fewer prey than control- or BSTI-prey-fed individuals. Females in all treatments gained more mass and survived longer than males. Low exposure to the proteins because of dilution and deactivation within the prey is the most likely explanation for the lack of tri-trophic effects observed. Aditionally, the presence of a digestive chymotrypsin only partially inhibited by BSTI may provide an alternative path for proteolysis.


Assuntos
Avidina/metabolismo , Besouros/efeitos dos fármacos , Nicotiana/genética , Nicotiana/metabolismo , Comportamento Predatório/efeitos dos fármacos , Inibidores da Tripsina/metabolismo , Animais , Avidina/genética , Avidina/farmacologia , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Proteínas de Transporte/farmacologia , Feminino , Larva/efeitos dos fármacos , Masculino , Mariposas/efeitos dos fármacos , Controle Biológico de Vetores , Plantas Geneticamente Modificadas , Reprodução/efeitos dos fármacos , Fatores de Tempo , Nicotiana/parasitologia , Inibidores da Tripsina/farmacologia
10.
Insect Mol Biol ; 16(6): 675-90, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18092997

RESUMO

The midgut is a key tissue in insect science. Physiological roles include digestion and peritrophic membrane function, as well as being an important target for insecticides. We used an expressed sequence tag (EST) approach to identify candidate genes and gene families involved in these processes in the light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae). Two cDNA libraries were constructed from dissected midgut of third to fifth instar larvae. Clustering analysis of 6416 expressed sequence tags produced 1178 tentative unique genes comprising 725 tentative contigs and 453 singletons. The sequences show similar codon usage to sequences from other lepidopterans, a Kozak consensus sequence similar to Drosophila and single nucleotide polymorphisms (SNPs) were detected at a frequency of 1.35/kb. The identity of the most common Interpro families correlates well with major known functions of the midgut. Phylogenetic analysis was conducted on representative sequences from selected multigene families. Gene families include a broad range of digestive proteases, lipases and carbohydrases that appear to have degradative capacity against the major food components found in leaves, the diet of these larvae; and carboxylesterases, glutathione-S-transferases and cytochrome P450 monooxygenases, potentially involved in xenobiotic degradation. Two of the larger multigene families, serine proteases and lipases, expressed a high proportion of genes that are likely to be catalytically inactive.


Assuntos
Lepidópteros/genética , Aminopeptidases/genética , Animais , Sequência de Bases , Carboxipeptidases/genética , DNA Complementar/genética , Sistema Digestório/metabolismo , Etiquetas de Sequências Expressas , Biblioteca Gênica , Genes de Insetos , Proteínas de Insetos/genética , Metabolismo dos Lipídeos , Repetições Minissatélites , Família Multigênica , Filogenia , Serina Endopeptidases/genética
11.
J Insect Physiol ; 48(12): 1093-1101, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12770032

RESUMO

To investigate the potential non-target impacts of transgenic pest-resistant plants, prey-mediated impacts of a protease inhibitor (PI) on the predatory carabid, Nebria brevicollis, were investigated. The PI used was aprotinin, a serine PI of mammalian origin with insecticidal properties when incorporated in artificial diet or expressed in transgenic plants. Field-collected N. brevicollis adults, kept at 23 degrees C, 16:8 L:D, were fed, over their pre-aestivation activity period of 24 days, with Helicoverpa armigera larvae reared on an artificial diet containing 0.5% (w:w, fresh mass) aprotinin. These larvae contained 22.62 &mgr;g aprotinin/g insect. Control prey was reared on diet without aprotinin. Beetle survival and body mass were unaffected by prey type. Beetles consuming PI-fed prey lost significantly more mass than the control beetles during two periods of mass loss, but gained significantly more mass during the final period of mass gain. This was not due to differences in amounts of prey supplied or consumed. The final mass gain coincided with increased consumption of PI-prey. Female beetles were significantly heavier than males, but we found no consistent gender-based differences in response to PI-prey. At the end of the experiment, body mass of all beetles was similar to field-collected ones (approximately 55 mg). All experimental beetles had significantly lower activities of digestive cysteine proteases and the serine proteases chymotrypsin and trypsin than field-collected ones. Beetles consuming PI-fed prey had significantly lower levels of trypsin and higher levels of chymotrypsin and elastase than the control beetles.

12.
J Insect Physiol ; 48(3): 327-336, 2002 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12770107

RESUMO

Ultrastructural changes to the midgut epithelium of nymphs of the black field cricket (Teleogryllus commodus) after ingestion of potato protease inhibitor II (PPI-II) (0.6% (w/v) in artificial diet) were determined by light and electron microscopy. Crickets fed diet containing PPI-II grew more slowly than those fed control diet and changes observed to the PPI-II-fed nymphs included reduction of midgut wall depth, vacuolisation of the epithelial cells, swelling of the microvilli, cellular protrusions into the midgut and eventual rupture of individual or small groups of epithelial cells. These changes were first seen 2 days after PPI-II ingestion. Complete disintegration of the midgut to the basement membrane was not seen during the 27-day observation period and repair and regeneration of pockets of epithelial cells was observed. Immunocytochemistry revealed that PPI-II was localised within the ectoperitrophic matrix space of the gut. The location of the peritrophic matrix was determined by labelling with wheat germ agglutinin (WGA), but no rupture of this structure was observed in PPI-II-fed nymphs.

13.
Lett Appl Microbiol ; 32(1): 36-41, 2001 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11169039

RESUMO

In two of eight Hofmannophila pseudospretella specimens studied by microscopy, the larval midgut contained an unidentified micro-organism. Although not seen microscopically in midgut sections, bacteria were isolated from dissected midgut. Microscopy, carbohydrate utilization and ribosomal sequence data all separated the isolates into the same three classes. These were identified as Lactococcus lactis, Carnobacterium piscicola and, tentatively, Bacillus subtilis, the first two being facultative anaerobes and the latter, an aerobe. The bacteria were therefore not specifically adapted to the reducing conditions of the midgut.


Assuntos
Bactérias/classificação , Bactérias/isolamento & purificação , Sistema Digestório/microbiologia , Larva/microbiologia , Mariposas/microbiologia , Animais , Bacillus subtilis/classificação , Bacillus subtilis/genética , Bacillus subtilis/isolamento & purificação , Bactérias/genética , Metabolismo dos Carboidratos , DNA Ribossômico/análise , Lactococcus lactis/classificação , Lactococcus lactis/genética , Lactococcus lactis/isolamento & purificação , Microscopia Eletrônica , Mariposas/crescimento & desenvolvimento , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
14.
Eur J Biochem ; 254(1): 160-7, 1998 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-9652409

RESUMO

Phloem exudate from squash fruit contains heat-inactivated material which inhibits pepsin activity. This inhibitory activity was purified by mild acid treatment, chromatography on trypsin-agarose, Sephadex G-75 and reverse-phase HPLC, resulting in the elution of three peaks with pepsin-inhibitory activity. N-terminal sequencing indicated a common sequence of MGPGPAIGEVIG and the presence of minor species with seven- or two-amino-acid N-terminal extensions beyond this point. Microheterogeneity in this end sequence was exhibited within and between two preparations. Internal sequencing of a major peak after a trypsin digestion gave the sequence FYNVVVLEK. The common N-terminal sequence was used to design a degenerate primer for 3' rapid amplification of cDNA ends and cDNA clones encoding two isoforms of the inhibitor were obtained. The open reading frames of both cDNAs encoded proteins (96% identical) which contained the experimentally determined internal sequence. The amino acid content calculated from the predicted amino acid sequence was very similar to that measured by amino acid analysis of the purified inhibitor. The two predicted amino acid sequences (96 residues) had neither similarity to any other aspartic proteinase inhibitor nor similarity to any other protein. The inhibitors have a molecular mass of 10,552 Da, measured by matrix-assisted laser-desorption ionisation time-of-flight mass spectrometry and approximately 10,000 Da by SDS/PAGE, and behave as dimers of approximately 21,000 Da during chromatography on Superdex G-75 gel-filtration medium. The calculated molecular masses from the predicted amino acid sequences were 10,551 Da and 10,527 Da. The inhibitor was capable of inhibiting pepsin (Ki = 2 nM) and a secreted aspartic proteinase from the fungus Glomerella cingulata (Ki = 20 nM). The inhibitor, which is stable over acid and neutral pH, has been named squash aspartic proteinase inhibitor (SQAPI).


Assuntos
Ácido Aspártico Endopeptidases/antagonistas & inibidores , Cucurbitaceae/química , Proteínas de Plantas/química , Aminoácidos/análise , Clonagem Molecular , Peso Molecular , Pepsina A/antagonistas & inibidores , Inibidores de Proteases/química , Alinhamento de Sequência , Análise de Sequência de DNA , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
15.
J Insect Physiol ; 44(2): 141-147, 1998 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12769886

RESUMO

Potato protease inhibitors, POT-1 and POT-2, were fed to newly emerged adult honey bees in cages at different doses in either sugar syrup (0.2 or 0.01% w:v) or pollen food (1 or 0.2% w:w). In vivo activities of three digestive endopeptidases (trypsin, chymotrypsin and elastase) and one exopeptidase (leucine aminopeptidase; LAP) were measured after 3 or 8days' exposure of bees to inhibitor. Enzyme activities were significantly lower at day 8 than at day 3, except for elastase, which did not change. POT-2 significantly reduced the activity of all endopeptidases at both timepoints, regardless of the dose level or the medium in which the inhibitor was administered. POT-1 acted in a similar manner, except that 0.01% POT-1 in syrup had no effect on bees. There was no consistent trend in changes in LAP activity. Bees fed either inhibitor at 1% in pollen or at 0.2% in syrup had significantly reduced lifespans, with the effect of the pollen treatment being greater than the syrup treatment. The survival of bees fed POT-1 or POT-2 at 0.2% in pollen or 0.01% in syrup did not differ from the controls.

16.
Plant Physiol ; 114(2): 715-722, 1997 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12223739

RESUMO

A glutamyl proteinase was partially purified from Percoll gradient-purified spinach (Spinacia oleracea) chloroplast preparations and appeared to be predominantly localized in the chloroplast stroma. The enzyme degraded casein, but of the 11 synthetic endopeptidase substrates tested, only benzyloxycarbonyl-leucine-leucine-glutamic acid-[beta]-napthylamide was hydrolyzed at measurable rates. In addition, the enzyme cleaved the oxidized [beta]-chain of insulin after a glutamic acid residue. There was no evidence that native ribulose-1,5-bisphosphate carboxylase/oxygenase was cleaved by this proteinase. The apparent Km for benzyloxycarbonyl-leucine-leucine-glutamic acid-[beta]NA at the pH optimum of 8.0 was about 1 mM. Cl-ions were required for both activity and stability. Of the proteinase inhibitors covering all four classes of the endopeptidases, only 4-(2-aminoethyl)-benzenesulfonyl-fluoride HCl and L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone significantly inhibited the proteinase. The partially purified enzyme had a molecular weight of about 350,000 to 380,000, based on size-exclusion chromatography. The enzyme has both similar and distinctive properties to those of the bacterial glutamyl proteinases. To our knowledge, this is the first description of a plant glutamyl proteinase found predominantly or exclusively in the chloroplast.

17.
Insect Biochem Mol Biol ; 27(11): 929-44, 1997 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9501417

RESUMO

A Helicoverpa armigera larval midgut cDNA library from larvae raised on an artificial, protein-rich, inhibitor-free diet contained very large numbers of serine proteinase positive clones. DNA sequencing of six random positive cDNAs and 12 PCR derived products identified trypsin genes classifiable into three families, and chymotrypsin and elastase genes classifiable into a single family each. Genomic blots established that the most highly expressed of the trypsin families contained about 18 genes, and that the chymotrypsin and elastase families contained about 14 and 2 genes respectively. The levels of mRNA corresponding to the highly expressed trypsin and chymotrypsin families were determined following chronic ingestion of four proteinase inhibitors. Compared to insects on an inhibitor-free diet, chymotrypsin mRNA was increased by all inhibitors, and trypsin mRNA levels decreased. This occurred independent of whether the inhibitor was solely a trypsin inhibitor (aprotinin), an inhibitor of both trypsin and chymotrypsin (proteinase inhibitor II, soybean trypsin inhibitor) or predominantly a chymotrypsin inhibitor (proteinase inhibitor I). Changing the protein level of the diet did not affect trypsin mRNA levels, but chymotrypsin mRNA levels decreased with increasing dietary protein.


Assuntos
Sistema Digestório/enzimologia , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Lepidópteros/enzimologia , Serina Endopeptidases/biossíntese , Serina Endopeptidases/genética , Inibidores de Serina Proteinase/farmacologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Quimotripsina/biossíntese , Sequência Conservada , DNA Complementar , Genes de Insetos , Larva , Lepidópteros/genética , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , RNA Mensageiro/biossíntese , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Serina Endopeptidases/química , Tripsina/biossíntese
18.
J Insect Physiol ; 43(9): 833-842, 1997 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12770495

RESUMO

The ornamental tobacco (Nicotiana alata) produces one 6-kDa chymotrypsin inhibitor and four 6-kDa trypsin inhibitors from a single 40.3-kDa precursor protein. Three different approaches have been used to assess the potential of these proteinase inhibitors (PIs) in insect control. The first was an in-vitro approach in which all five inhibitors, the single chymotrypsin inhibitor or three of the four trypsin inhibitors were tested for their ability to inhibit gut protease activity in insects from four orders. The second approach was to incorporate the N. alata PIs in the artificial diet of the native budworm (Helicoverpa punctigera) and the black field cricket (Teleogryllus commodus). H. punctigera larvae and T. commodus nymphs had a significant (P<0.01) reduction in growth after ingestion of the PI and were more lethargic than insects on the control diet. Several of the H. punctigera larvae also failed to complete moulting at the third or fourth instar. The third approach was to express the N. alata PIs in transgenic tobacco under the control of the 35S CaMV promoter. When H. punctigera larvae were fed tobacco leaves expressing the N. alata PIs at 0.2% soluble protein, significant (P<0.01) differences in mortality and/or growth rate were observed.

19.
Biol Chem Hoppe Seyler ; 376(5): 281-7, 1995 May.
Artigo em Inglês | MEDLINE | ID: mdl-7662170

RESUMO

The major trypsin inhibitor from pumpkin (Cucurbita maxima cv Supermarket Hybrid) fruit phloem exudate was purified by affinity and reverse phase chromatography. The protein has a molecular weight of approximately 8100 by SDS-PAGE and is blocked at the N-terminal serine. Following sequencing of a CNBr fragment, 3'- and 5'-RACE were used to isolate full length cDNAs corresponding to a trypsin inhibitor and to two chymotrypsin inhibitors. The three genes are similar, both in their translated and non-translated regions. Comparison of the full length translated proteins show that they are members of the proteinase inhibitor I family and almost identical apart from the P1 site in the proteinase binding loop. The genes encode proteins of 67 amino acids and appear to lack not only both pre- and prepro-peptide sequences but also the single disulphide present in most proteinase inhibitor I family members.


Assuntos
Quimotripsina/antagonistas & inibidores , DNA de Plantas/metabolismo , Exsudatos e Transudatos/química , Plantas/química , Inibidores da Tripsina/isolamento & purificação , Sequência de Aminoácidos , Sequência de Bases , Cromatografia de Afinidade , Clonagem Molecular , DNA de Plantas/isolamento & purificação , Eletroforese em Gel de Poliacrilamida , Dados de Sequência Molecular , Sondas de Oligonucleotídeos , Reação em Cadeia da Polimerase , Inibidores da Tripsina/química
20.
Magn Reson Imaging ; 13(3): 471-9, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-7791557

RESUMO

We propose a simple method of distinguishing Zeeman broadening arising from susceptibility inhomogeneity and chemical shift variation, applicable to NMR microscopy. The method is based on the use of a specially built probe-head in which orthogonal sample alignment is possible using the same radiofrequency (RF) coil. This allows the investigation of alignment effects in image distortion and relies on the fact that the isotropic chemical shift is invariant under reorientation, whereas the susceptibility-related local field will depend strongly on relative orientation of bounding surfaces with the external polarizing field. We apply this approach to the study of a simple phantom, and an insect larva (Spodoptera litura Fabricius), demonstrating in the latter case that susceptibility variations are sufficiently small to allow chemical shift imaging on a scale greater than 1 ppm.


Assuntos
Espectroscopia de Ressonância Magnética/métodos , Microscopia/métodos , Spodoptera/metabolismo , Animais , Larva/metabolismo , Modelos Estruturais
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