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1.
Antonie Van Leeuwenhoek ; 105(5): 987-94, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24664663

RESUMO

A Gram-negative, aerobic, non-motile and rod-shaped bacterial strain, D-17(T), was isolated from mud flats in the Yellow Sea in Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain D-17(T) belongs to the genus Pseudoruegeria and it shared 97.5 % similarity with the type strain of Pseudoruegeria haliotis WM67(T). The sequence similarities with Pseudoruegeria litimaris HD-43(T) and Pseudoruegeria aquimaris SW-255(T) were 96.9 and 96.1 %, respectively. Strain D-17(T) was found to grow with 0.5-6 % (w/v) NaCl, at 20-30 °C, and at pH 6.5-8.0. Strain D-17(T) was determined to contain Q-10 as the predominant ubiquinone and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c, as defined by the MIDI system) as the major fatty acids. The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid, an unidentified lipid and four unidentified phospholipids. The DNA G+C content was determined to be 63.6 mol%. The DNA-DNA relatedness with P. haliotis WM67(T) was 32.5 %. The differential phenotypic properties revealed that strain D-17(T) can be separated from other Pseudoruegeria species. Based on the data presented in this study, strain D-17(T) represents a novel species, for which the name Pseudoruegeria limi sp. nov. is proposed. The type strain is D-17(T) (=KCTC 32460(T) =JCM 19487(T)).


Assuntos
Sedimentos Geológicos/microbiologia , Rhodobacteraceae/classificação , Rhodobacteraceae/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Coreia (Geográfico) , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Oceanos e Mares , Fosfolipídeos/análise , Filogenia , Quinonas/análise , RNA Ribossômico 16S/genética , Rhodobacteraceae/genética , Rhodobacteraceae/fisiologia , Análise de Sequência de DNA
2.
J Microbiol ; 51(6): 736-40, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24385349

RESUMO

A Gram-staining-negative, non-motile, catalase- and oxidasepositive bacterium, designated strain I-15(T), was isolated from a crab of the Yellow Sea, Korea. On the basis of a 16S rRNA gene sequence analysis, strain I-15(T) was shown to belong to Bacteroidetes, related to the genus Aestuariibaculum. Sequence similarity between strain I-15(T) and the only type strain of the genus Aestuariibaculum, Aestuariibaculum suncheonense SC17(T), was 96.7%. Strain I-15(T) grew at 0.5-6.0% (w/v) NaCl, at 10-42°C and at pH 4.5-8.0. It could hydrolyze starch and Tweens 80. Menaquinone-6 was the only respiratory quinone, and summed features 3 (C16:1 ω7c/C16:1 ω6c) (16.4%), iso-C15:0 (15.6%), and iso-C15:1 G (12.6%) were the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 39.0 mol%. Polyphasic data allowed genotypic and phenotypic distinction of strain I-15(T) from the only validly published Aestuariibaculum species. Therefore, the organism is considered a novel species of the genus Aestuariibaculum, for which the name Aestuariibaculum scopimerae sp. nov. is proposed. The type strain is I-15(T) (=KCTC 32459(T) =JCM 19486(T)).


Assuntos
Braquiúros/microbiologia , Flavobacteriaceae/classificação , Flavobacteriaceae/isolamento & purificação , Animais , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/metabolismo , Flavobacteriaceae/genética , Flavobacteriaceae/metabolismo , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética
3.
J Environ Biol ; 29(4): 581-4, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19195401

RESUMO

Three species of the unrecorded octopus and squids, Octopus megalops, Berryteuthis magister and Gonatopsis makko were collected for the first time from the East/Japan Sea in June, 2005. New Korean names proposed for these three species are "Big-eye octopus, Mako gonate squid, Schoolmater gonate squid" for the O. megalops, B. magister and G. makko, respectively. We report detailed taxonomic descriptions of these species.


Assuntos
Cefalópodes/classificação , Cefalópodes/fisiologia , Animais , Cefalópodes/anatomia & histologia , Geografia , Japão , Oceano Pacífico , Frutos do Mar , Especificidade da Espécie
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