The Impact of Laser Thermal Effect on Histological Evaluation of Oral Soft Tissue Biopsy: Systematic Review.

The aim of the study is to review the literature to observe studies that evaluate the extent of the thermal effect of different laser wavelengths on the histological evaluation of oral soft tissue biopsies. An electronic search for published studies was performed on the PubMed and Scopus databases between July 2020 and November 2022. After the selection process, all the included studies were subjected to quality assessment and data extraction processes. A total of 28 studies met the eligibility criteria. The most studied laser was the carbon dioxide (CO2) laser, followed by the diode laser 940 nm-980 nm. Six studies were focused on each of the Erbium-doped Yttrium Aluminium Garnet (Er:YAG), Neodymium-doped Yttrium Aluminum Garnet (Nd:YAG) lasers, and diode lasers of 808 nm and 445 nm. Three studies were for the Potassium Titanyl Phosphate (KTP) laser, and four studies were for the Erbium, Chromium-doped Yttrium, Scandium, Gallium, and Garnet (Er,Cr:YSGG) laser. The quality and bias assessment revealed that almost all the animal studies were at a low risk of bias (RoB) in the considered domains of the used assessment tool except the allocation concealment domain in the selection bias and the blinding domain in the performance bias, where these domains were awarded an unclear or high score in almost all the included animal studies. For clinical studies, the range of the total RoB score in the comparative studies was 14 to 23, while in the non-comparative studies, it was 11 to 15. Almost all the studies concluded that the thermal effect of different laser wavelengths did not hinder the histological diagnosis. This literature review showed some observations. The thermal effect occurred with different wavelengths and parameters and what should be done is to minimize it by better adjusting the laser parameters. The extension of margins during the collection of laser oral biopsies and the use of laser only in non-suspicious lesions are recommended because of the difficulty of the histopathologist to assess the extension and grade of dysplasia at the surgical margins. The comparison of the thermal effect between different studies was impossible due to the presence of methodological heterogeneity.

A Systematic Review of Cementation Techniques to Minimize Cement Excess in Cement-Retained Implant Restorations.

BACKGROUND: The most used types of retention of implant-supported prostheses are screw-retained or cement-retained restorations. The advantages and disadvantages of both have been identified by various authors over the years. However, cement-retained implant crowns and fixed partial dentures are among the most used types of restorations in implant prostheses, due to their aesthetic and clinical advantages. When cemented prostheses are made on implants, the problem of cement residues is important and often associated with biological implant pathologies. The objective of this research was to establish to what extent the techniques to reduce excess cement really affect the volume of cement residues. MATERIALS AND METHODS: This review was written following the PRISMA statement; a detailed search was carried out in three different electronic databases-PubMed, Scopus, and Cochrane Library. The inclusion criteria were prospective clinical studies, with at least 10 participants per group, and with at least 6 months of the follow-up period. RESULTS: There have been many proposals for techniques supposed to reduce the amount of excess cement in the peri-implant sulcus and on the prosthetic components, but of these, which are exceptional in their in vitro capabilities, very few have been clinically validated, and this represents the real limitation and a great lack of knowledge regarding this topic. Three articles met the inclusion criteria, which were analyzed and compared, to obtain the information necessary for the purposes of the systematic review. DISCUSSION: Extraoral cementation can reduce the excess cement, which, after a normal excess removal procedure, is, nevertheless, of such size that it does not affect the possibility of peri-implant pathologies developing. All these studies concluded that a small amount of cement residue is found in the gingival sulcus, and using eugenol-free oxide cements, the residues were only deposited on the metal surfaces, with a better peri-implant tissues health. CONCLUSION: Despite the limitations of this study, it was possible to carefully analyze these characteristics and obtain valuable suggestions for daily clinical practice. Resinous cements are considered, due to the free monomers present in them, toxic for the soft tissues. The provisional zinc-oxide cements, also eugenol-free, represent the ideal choice. The different grades of retentive forces provided by these cements do not seem to have clinical effects on the decementation of restorations.

In Silico Novel Drug Design Targeting the Oral Microbiome: Endodontic and Periodontal Pathogenic Bacteria.

The oral cavity is composed of about 300 million species of bacteria that occupy various ecological niches [...].

Ultrasound Imaging in Dentistry: A Literature Overview.

(1) Background: the frequency with which diagnostic tests are prescribed with exposure to ionizing radiation, a cause of biological damage, has been studied, and with much more attention, patients are subjected to these diagnostic tests for diagnosis and follow-up. This review aimed, given the recent developments of this technology, to evaluate the possible use of ultrasound in different branches of dentistry. The possibility of applying ionizing-radiation-free diagnostic exams in dentistry, overcoming the limits of this application, has led scientific research in this area to obtain interesting results that bode well for the future. (2) Methods: a search for articles on the application of ultrasounds in dentistry was performed using the PubMed electronic database. (3) Results: only 32 studies were included, and these clearly stated that this examination is widely usable and in great progress. (4) Conclusions: regarding the modern application techniques of this diagnostic test, it is essential to consider technological evolution as an objective to reduce the damage and side effects of necessary diagnostic tests. The use of ultrasound in dentistry can represent a valid radiation-free alternative, in certain contexts, to the other most used exams.

An Update of the Possible Applications of Magnetic Resonance Imaging (MRI) in Dentistry: A Literature Review.

This narrative review aims to evaluate the current evidence for the application of magnetic resonance imaging (MRI), a radiation-free diagnostic exam, in some fields of dentistry. BACKGROUND: Radiographic imaging plays a significant role in current first and second level dental diagnostics and treatment planning. However, the main disadvantage is the high exposure to ionizing radiation for patients. METHODS: A search for articles on dental MRI was performed using the PubMed electronic database, and 37 studies were included. Only some articles about endodontics, conservative dentistry, implantology, and oral and craniofacial surgery that best represented the aim of this study were selected. RESULTS: All the included articles showed that MRI can obtain well-defined images, which can be applied in operative dentistry. CONCLUSIONS: This review highlights the potential of MRI for diagnosis in dental clinical practice, without the risk of biological damage from continuous ionizing radiation exposure.

Lingual Flap Protection during Third Molar Surgery: A Literature Review.

The purpose of this review was to analyze how the retraction and protection of lingual flap (LF+) could influence the incidence of lingual nerve injury (LNI) during third molar extraction, as compared with protocols that do not involve handling of lingual tissue (LF). A literature review was performed from the "Medline" and "Scopus" medical databases, using the keywords "lingual nerve" and "third molar surgery." From the selected articles, the mean values for transitory and permanent LNI's incidence were elaborated, taking into account the group treated with LF+ technique and the group treated with LF technique. Of 480 articles, 11 studies were included in the review. The LF+ group counted 3,866 surgeries and it resulted in a transitory LNI's mean incidence of 2.98 ± 0.03% and a mean incidence of 0.1 ± 0.003% for permanent LNI. The LF group counted 5,938 surgeries with, respectively, 1.92 ± 0.02 and 0.49 ± 0.006% of transitory and permanent LNI's incidence mean values. The results of this study suggest that the application of LF+ techniques reduces the risk of damage and injuries of lingual nerve.

Five Years Follow-up of Short Implants Placed in Atrophic Maxilla with Simultaneous Sinus Floor Transcrestal Elevation.

OBJECTIVE: Many authors have tried to face the anatomical limitations resulting from maxillary bone atrophy. Up to five millimeters bone height, the lateral sinus floor elevation is the most commonly used and validated strategy to achieve the prosthetic rehabilitation. However, the disadvantages of this technique are its invasiveness and delayed rehabilitation. The aim of this paper was to assess 5 years clinical outcome of implants placed with a technique that allows the percrestal sinus floor elevation and the immediate implant placement. MATERIALS AND METHODS: 30 transcrestal sinus floor elevations with immediate implant placement were performed in severely atrophic maxillae. Implant survival, marginal bone level variation, harvested bone height variation and periodontal indices were assessed. RESULTS: After a five year follow up none of the thirty implants were lost. The mean value of vertical harvested bone loss was 5%. The mean crestal bone loss was -0.33 mm (Standard Deviation (SD) 0.11 mm). The mean value of periodontal indices was respectively: PD 1.22 mm (SD 0.72 mm), PI 17.47% (SD 15.01 mm), BOP 9, 87%.,(SD 19.17 mm). CONCLUSION: The results obtained are comparable with success criteria in implant rehabilitation. The reported technique proved to be successful in the population observed, with minimal trauma and reduced invasiveness.

Operative Torque Analysis to Evaluate Cutting Efficiency of Two Nickel-Titanium Rotary Instruments for Glide Path: An In Vitro Comparison.

AIM AND OBJECTIVE: The aim and objective of this study was to evaluate and compare the cutting efficacy of two different nickel titanium rotary instruments by a novel methodology: the operative torque (torque and time needed to progress toward the apex). MATERIALS AND METHODS: Ten fresh extracted upper first premolars with two canals were instrumented with a KaVo (Biberach, Germany) and a KaVo 1:1 handpiece at 300 rpm with maximum torque set at 2 N. One canal was instrumented with ProGlider NiTi rotary instruments (Dentsply Sirona Endodontics, Ballaigues, Switzerland), with tip size of 16.02, and the other one with EgdeGlidePath rotary instrument (EdgeEndo, Albuquerque, New Mexico). Mean instrumentation time, mean torque values, and maximum torque values were evaluated for each instrument. The significance level was set at p <0.05. RESULTS: EdgeGlidePath instruments reached the working length in significantly less time with a significantly smaller amount of torque when compared to ProGlider (p >0.05). No instruments exhibited flute deformation or underwent intracanal failure. CONCLUSIONS: Operative torque is related to the capability to cut dentin and progress toward the apex: the smaller the torque values, the higher the cutting ability (and safety). Operative torque is also dependent on debris removal and irrigation techniques. Nevertheless, both operative torque and instrumentation time are clinically relevant parameters for evaluating instruments' performance (i.e., cutting ability). CLINICAL SIGNIFICANCE: Operative torque during endodontic instrumentation helps understanding the overall performance in terms of both cutting efficiency and safety.

An Update of Possible Radiation-free Imaging Techniques in Dentistry.

Cone-beam computed tomography (CBCT) represents today the most widespread and most used 3D examination in dentistry. It is precise because of its usefulness in orthodontics and orthognathic surgery with large field of view (FOVs); in oral surgery and implantology for the 3D evaluation of bone volumes and proximity to noble structures; and in endodontics for the understanding of the often difficult root canal system anatomy with reduced FOV and greater resolution that this imaging technique, also considering the wide diffusion and availability in dental offices, in addition to the short examination execution times, allows its wide use.1-4.

Mechanical Characteristics, Testing, and Future Perspective of Nickel-Titanium Rotary Instruments.

The main drawback of several studies is the limited analysis performed for the comparison of nickel-titanium (NiTi) rotary systems. Most often, research articles in this area are focalized on the static characteristics such as static cyclic fatigue, torsional loads, and flexibility, not considering their reciprocal interaction and all dynamic factors.1 Recent evidence in the literature defines the reciprocal interaction between these parameters as a fundamental element in the study of their mechanical characteristics.2 However, static mechanical behavior is fundamental to assess the basic performance of NiTi instruments, but its interpretation during clinical practice is pointless as it does not resemble the real clinical scenario.1,3,4 Results arising from static tests, certainly valid as a first approach to the evaluation of an instrument recently introduced on the market, and comparative evaluations, should be considered in a general and more complete view, considering their reciprocal relationships.3-6 For these reasons, different types of researches that investigate both static and dynamic performance of NiTi instruments should be strongly recommended in order to give to original research and in vitro study an actual clinical significance, certainly much more indicative in view of a subsequent clinical application of NiTi rotary instruments.7-9.

Nonsurgical Retreatment Using Regenerative Endodontic Protocols: A Case Report.

AIM AND OBJECTIVE: The aim of this case report was to describe regenerative endodontic procedures (REPs) of the previously treated mature teeth with long-term results. BACKGROUND: Regenerative endodontic procedures are aimed to treat apical periodontitis and regenerate the pulp-dentin complex in necrotic teeth. However, there is no consensus in using REPs in the previously treated mature teeth. The aim of this case report was to describe REPs of the previously treated mature teeth with long-term results. CASE DESCRIPTION: A 25-year-old woman presented pain on chewing on 15 and swelling (WHO numbering system). The tooth had been endodontically treated and restored 4 years ago. After removing the old root canal filing, the roots were irrigated with 2.5% sodium hypochlorite and 17% EDTA using sonic activation. Calcium hydroxide (CH) was used as medicament for 3 weeks. At the second visit, CH was removed, and canals were irrigated as like as the first visit. Apical bleeding was induced, and concentrated growth factors (CGFs) were placed inside the root canal. The tooth has been followed up to 3 years. CONCLUSION: The tooth was functional and asymptomatic at the recall visits. Since the first-year follow-up, the tooth responded to the electric pulp test and the thermal test. Radiological examinations revealed healing of apical lesion and hard tissue deposition. CLINICAL SIGNIFICANCE: Regenerative endodontic procedures can offer an advantage over traditional endodontic procedures in terms of tertiary healing, with a predictable, user-friendly procedure also for retreatment cases.

Successful Management of Peri-Implantitis around Short and Ultrashort Single-Crown Implants: A Case Series with a 3-Year Follow-Up.

INTRODUCTION AND AIM: In case of peri-implantitis, resective surgery is contraindicated for short and ultrashort implants, limiting the treatment options to regenerative surgery or to implant removal. This retrospective case series presents the clinical and radiographic outcomes of a surgical regenerative procedure to treat peri-implantitis around short and ultrashort implants. MATERIALS AND METHODS: The study is a retrospective evaluation of patients suffering from peri-implantitis and those who underwent access flap surgery, concomitant chemical and mechanical decontamination of implant surface, and bone grafting using a self-hardening mixture of bone substitutes and biphasic calcium sulfate. No membranes were applied to cover the grafting material, and primary tension-free closure was achieved. The retrospective protocol was reviewed and approved by the Ethics Committee for Clinical Sperimentation (CESC) of Verona and Rovigo, Italy (based in the University of Verona) (Prog. 1863CESC. Date of approval: 2018-07-04). RESULTS: 15 patients (17 implants) have been diagnosed with peri-implantitis after a mean follow-up of 24 months after loading. Implant length was between 5 and 8 mm. 8 patients (10 implants) had a history of periodontitis. At baseline, the mean PD (probing pocket dept) at the deepest site was 8.12 mm, with an average mBI (modified bleeding index) of 2.35 and a mean BD (bone defect depth) of 3.04 mm. At the 3-year follow-up, the CSR was 100%, the mean mBI was 0.88 (average reduction: -1.47), the mean PD was 3.35 mm (mean PD reduction: 4.77 mm), and the mean bone defect was reduced by 1.74 mm, with a mean bone fill of 55%. CONCLUSIONS: The results of the present case series suggest that if accurate surface decontamination is achieved, high survival rate and good clinical and radiographic results can be obtained after 3 years. However, only the histological examination could confirm the growth of new bone in direct contact with the implant surface or if the grafted material only fills the space left by the peri-implant defect.

Metal free, full arch, fixed prosthesis for edentulous mandible rehabilitation on four implants.

PURPOSE: The goal of this work is to describe an implant-prosthetic protocol for rehabilitation of edentulous mandible, by using a fixed prosthesis made of fiber-reinforced composite material (FRC). The protocol contemplates a minimal invasive surgery and ensures predictable and safe results, with good aesthetic and performance combined to cost savings. METHODS: FRC material is used to build the substructure of a prosthetic framework supported by four short implants (5mm long and 4mm wide). The prosthesis substructure is made of Trinia immersed in a matrix of epoxy resin (FRC). It is supplied in milling blocks (pre-cured) for the CAD/CAM (computer-aided design/computer-aided manufacturing) technique. Implants are placed in lower edentulous jaw in position of first molar and canine, each side. Four month after, a resin bar is build based on a stone model, denture teeth are placed and the occlusion is checked. The resin bar and the stone model with milled abutments are scanned and a FRC bar is achieved with the CAD/CAM technique. The teeth are mounted to the substructure trough denture resin. Temporary cementation of framework is achieved on the abutments connected to the implants. CONCLUSION: A protocol for a fixed mandibular implant-prosthetic rehabilitation is described. The protocol contemplates a minimal invasive surgery and ensures predictable and safe results, with good aesthetic and performance combined to cost savings. In addition, this technique allows performing basic surgery also in presence of atrophy.

An anatomical radiographic evaluation of the posterior portion of the mandible in relation to autologous bone harvest procedures.

The purpose of the study was to evaluate the course of the mandibular canal and the thickness of the vestibular cortical plate in the posterior region of the mandible in relation to autologous bone harvest procedures. The study was performed on a cohort of 30 cone-beam computed tomography hemimandible images. For each hemimandible, the course of the mandibular canal and the thickness of the vestibular cortical plate have been evaluated in 4 regions: the retromolar region, the second molar region, the first molar region, and the second premolar region. The analyzed variables show a characteristic trend: the thickness of the cortical vestibular plate and the horizontal distance of the canal from the cortical vestibular plate are higher in the second molar region specifically in the area bordering on the retromolar region. In fact, the maximum thickness reaches the average value of 3.46 mm on 30 hemimandibles for slice (SD, 0.56 mm; range, 2.36-4.83 mm), and the horizontal distance reaches the average value of 6.06 mm on 30 hemimandibles for slice (SD, 1.34 mm; range, 3.65-9.27 mm); both variables decrease in more distal slices of the retromolar region. The vertical distance of the canal from the cortical crest shows the average value of 14.25 mm on 22 slices of the second molar and retromolar regions (SD of average values, 1.03 mm; range of average values, 12.92-16.25 mm; range of absolute values, 7.11-22.92 mm) exactly in regions potentially suitable for procedures of bone harvest (second molar and retromolar regions).

Intraosseous vascularization of anterior mandible: a radiographic analysis.

The anterior mandible was initially considered a safe zone from a surgical point of view. Over time, serious complications resulting from dental implantology procedures have been reported. This has led to a greater focus on vascularization of the interforaminal region. The purpose of this article was to evaluate the anterior mandibular region in relation to the presence of canals perforating the buccal and lingual cortical plates. DICOM (Digital Imaging and Communications in Medicine) files of 100 cone beam computed tomography scans were analyzed by imaging software. All radiographic cross-sections between the mental foramina were examined to detect the presence and location of perforating buccal or lingual canals. Lingual perforating canals are present in 98% of the cohort. The most common site is the symphysis region. The presence of 2 lingual canals cranially and caudally to the genial apophysis has been found in 39%. Vestibular perforating canals are absent in 61%. The anterior mandible has several lingual perforating canals, which are variable in number and location. Imaging software is a valuable aid in presurgical planning. Because of the higher prevalence of perforating canals in the symphysis region, this site should be preserved.

Micro-CT and PET analysis of bone regeneration induced by biodegradable scaffolds as carriers for dental pulp stem cells in a rat model of calvarial "critical size" defect: Preliminary data.

Bone regeneration strategies in dentistry utilize biodegradable scaffolds seeded with stem cells able to induce bone formation. However, data on regeneration capacity of these tissue engineering constructs are still deficient. In this study micro-Computed tomography (micro-CT) and positron emission tomography (PET) analyses were used to investigate bone regeneration induced by two scaffolds [Granular deproteinized bovine bone (GDPB) and Beta-tricalcium phosphate (ß-TCP)] used alone or in combination with dental pulp stem cells (DPSC) in a tissue engineered construct implanted in a rat critical calvarial defect. Bone mineral density (BMD) and standard uptake value (SUV) of tracer incorporation were measured after 2, 4, 8, and 12 weeks post-implant. The results showed that: (1) GDPB implants were mostly well positioned, as compared to ß-TCP; (2) GDPB induced higher BMD and SUV values within the cranial defect as compared to ß-TCP, either alone or in combination with stem cells; (3) addition of DPSC to the grafts did not significantly induce an increase in BMD and SUV values as compared to the scaffolds grafted alone, although a small tendency to increase was observed. Thus our study demonstrates that GDPB, when used to fill critical calvarial defects, induces a greater percentage of bone formation as compared to ß-TCP. Moreover, this study shows that addition of DPSC to pre-wetted scaffolds has the potential to ameliorate bone regeneration process, although the set of optimal conditions requires further investigation.

Radiographic evaluation of the symphysis menti as a donor site for an autologous bone graft in pre-implant surgery.

PURPOSE: This study was performed to obtain a quantitative evaluation of the cortical and cancellous bone graft harvestable from the mental and canine regions, and to evaluate the cortical vestibular thickness. MATERIALS AND METHODS: This study collected cone-beam computed tomographic (CBCT) images of 100 Italian patients. The limits of the mental region were established: 5 mm in front of the medial margin of each mental foramen, 5 mm under the apex of each tooth present, and above the inferior mandibular cortex. Cortical and cancellous bone volumes were evaluated using SimPlant software (SimPlant 3-D Pro, Materialize, Leuven, Belgium) tools. In addition, the cortical vestibular thickness (minimal and maximal values) was evaluated in 3 cross-sections corresponding to the right canine tooth (3R), the median section (M), and the left canine tooth (3L). RESULTS: The cortical volume was 0.71±0.23 mL (0.27-1.96 mL) and the cancellous volume was 2.16±0.76 mL (0.86-6.28 mL). The minimal cortical vestibular thickness was 1.54±0.41 mm (0.61-3.25 mm), and the maximal cortical vestibular thickness was 3.14±0.75mm(1.01-5.83 mm). CONCLUSION: The use of the imaging software allowed a patient-specific assessment of mental and canine region bone availability. The proposed evaluation method might help the surgeon in the selection of the donor site by the comparison between bone availability in the donor site and the reconstructive exigency of the recipient site.

A comparative morphometric analysis of biodegradable scaffolds as carriers for dental pulp and periosteal stem cells in a model of bone regeneration.

Bone regeneration and bone fixation strategies in dentistry utilize scaffolds containing regenerating-competent cells as a replacement of the missing bone portions and gradually replaced by autologous tissues. Mesenchymal stem cells represent an ideal cell population for scaffold-based tissue engineering. Among them, dental pulp stem cells (DPSCs) and periosteal stem cells (PeSCs) have the potential to differentiate into a variety of cell types including osteocytes, suggesting that they can be used with this purpose. However, data on bone regeneration properties of these types of cells in scaffold-based tissue engineering are yet insufficient.In this study, we evaluated temporal dynamic bone regeneration (measured as a percentage of bone volume on the total area of the defect) induced by DPSCs or PeSCs when seeded with different scaffolds to fill critical calvarial defects in SCID Beige nude mice. Two commercially available scaffolds (granular deproteinized bovine bone with 10% porcine collagen and granular ß;-tricalcium phosphate) and one not yet introduced on the market (a sponge of agarose and nanohydroxyapatite) were used. The results showed that tissue-engineered constructs did not significantly improve bone-induced regeneration process when compared with the effect of scaffolds alone. In addition, the data also showed that the regeneration induced by ß;-tricalcium phosphate alone was higher after 8 weeks than that of scaffold seeded with the 2 stem cell lines. Altogether these findings suggest that further studies are needed to evaluate the potential of DPSCs and PeSCs in tissue construct and identify the appropriate conditions to generate bone tissue in critical-size defects.

3D imaging reconstruction and impacted third molars: case reports.

There is a debate in the literature about the need for Computed Tomagraphy (CT) before removing third molars, even if positive radiographic signs are present. In few cases, the third molar is so close to the inferior alveolar nerve that its extraction might expose patients to the risk of post-operative neuro-sensitive alterations of the skin and the mucosa of the homolateral lower lip and chin. Thus, the injury of the inferior alveolar nerve may represent a serious, though infrequent, neurologic complication in the surgery of the third molars rendering necessary a careful pre-operative evaluation of their anatomical relationship with the inferior alveolar nerve by means of radiographic imaging techniques. This contribution presents two case reports showing positive radiographic signs, which are the hallmarks of a possible close relationship between the inferior alveolar nerve and the third molars. We aim at better defining the relationship between third molars and the mandibular canal using Dental CT Scan, DICOM image acquisition and 3D reconstruction with a dedicated software. By our study we deduce that 3D images are not indispensable, but they can provide a very agreeable assistance in the most complicated cases.

Stem cells from oral niches: a review.

AIM: Stem cell research in recent years have been considered the most advanced sort of medical-scientific research and early results have aroused great expectations. Also in dentistry many studies were performed with the final aim of obtaining new bone and new teeth. In this work we describe the state of the art in dental science stem cell research. METHODS: We have performed a web-based research on MEDLINE within (www.pubmed.gov). We have used "stem cells from human exfoliated deciduous teeth" (24 paper found), "periodontal ligament stem cells" (32 paper found), "stem cell apical papilla" (16 paper found), "dental pulp stem cells" (136 paper found) as keywords for research. For each keyword we have performed a complete review focusing on knowledge upgrade. RESULTS: For each topic was created a selection of papers in chronological order of publication date so to give a timetable of the development of the research for each niche. CONCLUSION: Research about stem cell from oral niches began in 2000 and every year papers publicated were more than the precedent. This review analysed about 180 articles most of which in the last 5 years. Dentla pulp from adult as from deciduous teeth seems to be the most valuable font of stem cells due to the pluripotential type of cells.