Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
J Microbiol Methods ; 189: 106320, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34478762

RESUMO

Exposure to enteric pathogens in the environment poses a serious risk for infection and disease. The accurate detection and quantification of enteric pathogens in environmental samples is critical for understanding pathogen transport and fate and developing risk assessment models. In this study, we successfully applied TaqMan real-time PCR assays to quantitatively detect five human-specific pathogens (Shigella/EIEC, Salmonella Typhi, Vibrio cholera, Norovirus, and Giardia) in samples from open drains, canals, floodwater, septic tanks, and anaerobic baffled reactors (ABR) collected in Mirpur, Dhaka, Bangladesh from April to October 2019. Overall, the grab and sediment samples showed low inhibition but the ultrafiltration samples collected from open drain had significantly higher (P = 0.0049) degree of PCR inhibition (median Ct = 31.06) compared to the extraction controls (Ct = 28.54). We developed a two-step method to adjust underestimation of pathogen quantities due to PCR inhibition and non-optimum PCR efficiency. Compared to other sample types, ultrafiltration samples demonstrated a wide range of concentration increase (1.0%-182.5%) by pathogens after adjusting for PCR inhibition and non-optimum efficiencies. These quantitative qPCR assays are successful in quantifying multiple enteric pathogens in environmental samples, and the adjustment method would be useful for correcting underestimates of pathogen quantities due to partial PCR inhibition and non-optimum efficiency.


Assuntos
Genoma Bacteriano/genética , Genoma Viral/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Reação em Cadeia da Polimerase em Tempo Real/normas , Manejo de Espécimes/métodos , Bactérias/genética , Bactérias/isolamento & purificação , Microbiologia Ambiental , Fezes/microbiologia , Fezes/parasitologia , Fezes/virologia , Giardia/genética , Giardia/isolamento & purificação , Ultrafiltração , Vibrio cholerae/genética , Vibrio cholerae/isolamento & purificação , Vírus/genética , Vírus/isolamento & purificação , Águas Residuárias/microbiologia , Águas Residuárias/parasitologia , Águas Residuárias/virologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA