Unskilled and unaware: second-order judgments increase with miscalibration for low performers.

Overestimation and miscalibration increase with a decrease in performance. This finding has been attributed to a common factor: participants' knowledge and skills about the task performed. Researchers proposed that the same knowledge and skills needed for performing well in a test are also required for accurately evaluating one's performance. Thus, when people lack knowledge about a topic they are tested on, they perform poorly and do not know they did so. This is a compelling explanation for why low performers overestimate themselves, but such increases in overconfidence can also be due to statistical artifacts. Therefore, whether overestimation indicates lack of awareness is debatable, and additional studies are needed to clarify this issue. The present study addressed this problem by investigating the extent to which students at different levels of performance know that their self-estimates are biased. We asked 653 college students to estimate their performance in an exam and subsequently rate how confident they were that their self-estimates were accurate. The latter judgment is known as second-order judgments (SOJs) because it is a judgment of a metacognitive judgment. We then looked at whether miscalibration predicts SOJs per quartile. The findings showed that the relationship between miscalibration and SOJs was negative for high performers and positive for low performers. Specifically, for low performers, the less calibrated their self-estimates were the more confident they were in their accuracy. This finding supports the claim that awareness of what one knows and does not know depends in part on how much one knows.

Levels of Trust in Information Sources as a Predictor of Protective Health Behaviors During COVID-19 Pandemic: A UAE Cross-Sectional Study.

Health information sources and the level of trust in a particular source may influence the subsequent adoption of advocated health behaviors. Information source preference and levels of trust are also likely to be influenced by sociodemographic (culture, age, gender) variables. Understanding these source-trust-behavior relationships across various national and cultural contexts is integral to improved health messaging. The present study identified the sources most frequently consulted to obtain information about COVID-19 during the pandemic's early stages in the United Arab Emirates (UAE). The study quantified levels of trust across an array of information sources, factoring in sociodemographic variables. Finally, the study explored the relationship between sociodemographic variables, levels of trust in information sources, and the adoption of COVID-19 related protective behaviors. Participants (n = 1585) were recruited during the first 2 weeks of April 2020 via announcements in the UAE media and through email networks. All participants completed a web-based survey presented in English or Arabic, as preferred. The most frequently consulted information sources were websites (health information websites), social media, government communications, and family and friends. The sources rated most trustworthy were: personal physicians, health care professionals, and government communications. There were differences in the use of sources and levels of trust according to age, gender, and education. The levels of trust in sources of information were associated with the adoption of protective behaviors, significantly so for citizens of the UAE. These findings may help inform the improvement of pandemic-related health messaging in multicultural contexts.

Contextual cueing is not flexible.

Target detection is faster when search displays repeat, but properties of the memory representations that give rise to this contextual cueing effect remain uncertain. We adapted the contextual cueing task using an ABA design and recorded the eye movements of healthy young adults to determine whether the memory representations are flexible. Targets moved to a new location during the B phase and then returned to their original locations (second A phase). Contextual cueing effects in the first A phase were reinstated immediately in the second A phase, and response time costs eventually gave way to a repeated search advantage in the B phase, suggesting that two target-context associations were learned. However, this apparent flexibility disappeared when eye tracking data were used to subdivide repeated displays based on B-phase viewing of the original target quadrant. Therefore, memory representations acquired in the contextual cueing task resist change and are not flexible.

Dunning-Kruger Effect: Intuitive Errors Predict Overconfidence on the Cognitive Reflection Test.

The Cognitive Reflection Test (CRT) is a measure of analytical reasoning that cues an intuitive but incorrect response that must be rejected for successful performance to be attained. The CRT yields two types of errors: Intuitive errors, which are attributed to Type 1 processes; and non-intuitive errors, which result from poor numeracy skills or deficient reasoning. Past research shows that participants who commit the highest numbers of errors on the CRT overestimate their performance the most, whereas those with the lowest error-rates tend to slightly underestimate. This is an example of the Dunning-Kruger Effect (DKE). The present study examined how intuitive vs. non-intuitive errors contribute to overestimation in the CRT at different levels of performance. Female undergraduate students completed a seven-item CRT test and subsequently estimated their raw score. They also filled out the Faith in Intuition (FI) questionnaire, which is a dispositional measure of intuitive thinking. Data was separated into quartiles based on level of performance on the CRT. The results demonstrated the DKE. Additionally, intuitive and non-intuitive errors predicted miscalibration among low, but not high performers. However, intuitive errors were a stronger predictor of miscalibration. Finally, FI was positively correlated with CRT self-estimates and miscalibration, indicating that participants who perceived themselves to be more intuitive were worse at estimating their score. These results taken together suggest that participants who perform poorly in the CRT and also those who score higher in intuitive thinking disposition are more susceptible to the influences of heuristic-based cues, such as answer fluency, when judging their performance.

Experiential avoidance, committed action and quality of life: Differences between college students with and without chronic illness.

This study aimed to explore, through structural equation modelling, experiential avoidance and committed action's effects on the association between anxiety and psychological quality of life and whether this relationship presents significant differences across a sample of 115 college students with chronic illness and a sample of 232 students without illness. Students with chronic illness presented higher levels of anxiety and experiential avoidance and lower levels of quality of life. The association between anxiety and psychological quality of life was partially explained by experiential avoidance and committed action. This path model was shown to be invariant between the two groups of students.

Rhipicephalus microplus serpins interfere with host immune responses by specifically modulating mast cells and lymphocytes.

Rhipicephalus microplus ticks feed on a bovine host for three weeks. At the attachment site, inflammatory and immune responses are triggered resulting in the recruitment of cells and production of a set of immunological mediators. To oppose the host's immune responses, ticks inoculate bioactive salivary molecules capable of interfering with these defense mechanisms. Serpins are among the most frequent molecules present in tick saliva and have been shown to negatively affect the host's anti-tick immunity. R. microplus has at least eighteen full-length serpins (RmS) and eleven are transcribed during blood feeding. Among them, RmS-3, RmS-6, and RmS-17 are present in the saliva of engorged females. Here, the effect of these serpins on the immune responses was evaluated in cells involved in innate/inflammatory (mast cells and macrophages) and adaptive (T cells) immunity. RmS-3 modulated mast cells due to its inhibitory activity on peritoneal rat chymase and on vascular permeability in acute inflammation. In addition, both RmS-6 and RmS-17 inhibited vascular permeability. Of the three serpins studied, neither affected activation nor inflammatory cytokine production by murine macrophages. On the other hand, RmS-3 and RmS-17 presented an inhibitory effect on the metabolic activity of lymphocytes, with the latter being the most potent, while RmS-6 had no effect on it. This activity was associated with a decrease in lymphocyte proliferation, but not with induction of cell death. The present study highlights the powerful modulatory role of tick salivary serpins in the host's immune system and inspire the discovery of targets for the treatment of inflammatory/immune disorders.

Religious/spiritual coping and spiritual distress in people with cancer / El coping religioso/espiritual y la angustia espiritual en individuos con cáncer / Coping religioso/espiritual e a angústia espiritual em pessoas com câncer

ABSTRACT Objective: To investigate the relation between the presence of spiritual distress and use of RSC and sociodemographic, clinical and religious/spiritual variables in people with cancer. Method: Cross-sectional study conducted in an association for support to people with cancer. The data obtained with the tools were analyzed using the Spearman's correlation coefficient and the Mann-Whitney Test. Results: 129 volunteers participated in the study, of which 57% showed moderate spiritual distress, 96% used medium and high positive religious/spiritual coping. Spiritual distress showed positive correlation with negative religious/spiritual coping (P<0.001) and inverse correlation with age (p 0.002). The use of positive religious coping was statistically significant in people who have religious practices (p 0.001). Conclusão: Spiritual distress is a phenomenon that is present in the lives of people with cancer and has significant relation with the use, in a negative manner, of religion/spirituality as a way of coping with the disease.

RESUMEN Objetivo: Examinar la relación entre la presencia de angustia espiritual y el uso del coping religioso/espiritual con variables sociodemográficas, clínicas y de aspectos religioso/espiritual en individuos con cáncer. Método: Estudio transversal, realizado en una asociación de apoyo a la persona con cáncer. En el análisis de datos recolectados se utilizó el coeficiente de correlación de Spearman y el test de Mann-Whitney. Resultados: En el estudio participaron 129 voluntarios; el 57% presentó moderada angustia espiritual, y el 96% utilizaba medio y elevado coping religioso/espiritual positivo. La angustia espiritual presentó una correlación positiva con el coping religioso/espiritual negativo (P < 0,001) e inversa con la edad (p 0,002). Se observó que el uso del coping religioso positivo fue estadísticamente significativo en los sujetos que participan en prácticas religiosas (p 0,001). Conclusión: La angustia espiritual es un fenómeno presente en la vida de los individuos con cáncer y tiene una significativa relación con el uso negativo de la religión/espiritualidad como forma de luchar contra la enfermedad.

RESUMO Objetivo: Investigar a relação entre presença de angústia espiritual e uso do coping religioso/espiritual com variáveis sociodemográficas, clínicas e de aspectos religioso/espiritual em pessoas com câncer. Método: Estudo transversal, realizado em uma associação de apoio à pessoa com câncer. Os dados obtidos dos instrumentos foram analisados por meio do coeficiente de correlação de Spearman e do teste Mann-Whitney. Resultados: Participaram do estudo 129 voluntários; 57% apresentaram moderada angústia espiritual, e 96% faziam uso de médio e alto coping religioso/espiritual positivo. A angústia espiritual apresentou correlação positiva com o coping religioso/espiritual negativo (P < 0,001) e inversa com a idade (p 0,002). O uso do coping religioso positivo foi estatisticamente significativo em pessoas que desenvolvem práticas religiosas (p 0,001). Conclusão: A angústia espiritual é um fenômeno presente na vida de pessoas com câncer e tem significativa relação com o uso, de maneira negativa, da religião/espiritualidade como forma de enfrentamento da doença.

Religious/spiritual coping and spiritual distress in people with cancer.

OBJECTIVE: To investigate the relation between the presence of spiritual distress and use of RSC and sociodemographic, clinical and religious/spiritual variables in people with cancer. METHOD: Cross-sectional study conducted in an association for support to people with cancer. The data obtained with the tools were analyzed using the Spearman's correlation coefficient and the Mann-Whitney Test. RESULTS: 129 volunteers participated in the study, of which 57% showed moderate spiritual distress, 96% used medium and high positive religious/spiritual coping. Spiritual distress showed positive correlation with negative religious/spiritual coping (P<0.001) and inverse correlation with age (p 0.002). The use of positive religious coping was statistically significant in people who have religious practices (p 0.001). CONCLUSÃO: Spiritual distress is a phenomenon that is present in the lives of people with cancer and has significant relation with the use, in a negative manner, of religion/spirituality as a way of coping with the disease.

The impact of illness-related shame on psychological health and social relationships: Testing a mediational model in students with chronic illness.

This study explores the impact of illness-related shame on the quality of social relationships and psychological health in chronic patients. We aimed to examine the roles of fear of receiving compassion from others and experiential avoidance as potential mediators of this relationship. Although some studies have demonstrated the negative impact of chronic illness-related shame on psychological functioning, the mechanisms that may underlie this link remain understudied. The sample was comprised by 115 college students, which had been diagnosed with at least 1 chronic illness. Participants completed self-report measures on an online platform. This study's design was cross-sectional. A path analysis was conducted using structural equation modelling. Results showed that the impact of illness-related shame on both psychological health (R2  = .45) and the quality of social relationships (R2  = .33) was fully accounted by fear of compassion from others and experiential avoidance. This model revealed an excellent fit. Fear of receiving compassion from others was the main mediator of the illness-related shame link with the quality of social relationships (ß = -.22). The main mediator of the association between shame-related chronic illness and psychological health was experiential avoidance (ß = -.21).This study shed light on possible psychological mechanisms linking feelings of shame associated with having a chronic condition and impaired social relationships and mental health. On one hand, resisting feelings of compassion and care from others and, on the other hand, avoiding difficult internal experiences and situations that might trigger them seem to underlie the impact of shame on psychological and social functioning in chronic patients.

The dark side of dialog.

We agree that the self is constructed through a collaborative dialog. But hostile interlocutors could use various cognitive techniques to hijack the dialog, resulting in beliefs, values, and even selves that are out of line with reality. The implications of this problem are dire, but we suggest that increased metacognitive awareness could help guide this process to a truthful conclusion.

There Is No Impact of Diabetes on the Endothelial Function of Chronic Kidney Disease Patients.

BACKGROUND: Patients with chronic kidney disease (CKD) and type 2 diabetes mellitus (DM) have increased risk of endothelial dysfunction, cardiovascular disease, and mortality. Several studies have separately analyzed endothelial function in these populations. However, data of patients with both CKD and DM are scarce. The aim of this study was to evaluate whether the presence of DM has any additional effect on the endothelial dysfunction of CKD patients. METHODS: We measured endothelial progenitor cells (EPCs), stromal-derived factor 1 alpha (SDF-1α), serum and urinary nitric oxide (NO), flow-mediated dilation (FMD), and pulse wave velocity (PWV) in 37 CKD patients with DM (CKD-DM group) and in 37 without DM (CKD group). RESULTS: CKD-DM group had a higher prevalence of obesity (P < 0.01), previous myocardial infarction (P = 0.02), myocardial revascularization (P = 0.04), and a trend for more peripheral artery disease (P = 0.07). Additionally, CKD-DM group had higher EPC (P = 0.001) and PWV (P < 0.001) values. On the other hand, no difference in SDF-1α and serum or urinary NO and FMD was observed between the groups. CONCLUSIONS: Endothelial dysfunction is frequent in CKD patients, and an additive effect of diabetes cannot be implicated, suggesting the predominant role of uremia in this condition.

The putative role of Rhipicephalus microplus salivary serpins in the tick-host relationship.

Inflammation and hemostasis are part of the host's first line of defense to tick feeding. These systems are in part serine protease mediated and are tightly controlled by their endogenous inhibitors, in the serpin superfamily (serine protease inhibitors). From this perspective ticks are thought to use serpins to evade host defenses during feeding. The cattle tick Rhipicephalus microplus encodes at least 24 serpins, of which RmS-3, RmS-6, and RmS-17 were previously identified in saliva of this tick. In this study, we screened inhibitor functions of these three saliva serpins against a panel of 16 proteases across the mammalian defense pathway. Our data confirm that Pichia pastoris-expressed rRmS-3, rRmS-6, and rRmS-17 are likely inhibitors of pro-inflammatory and pro-coagulant proteases. We show that rRmS-3 inhibited chymotrypsin and cathepsin G with stoichiometry of inhibition (SI) indices of 1.8 and 2.0, and pancreatic elastase with SI higher than 10. Likewise, rRmS-6 inhibited trypsin with SI of 2.6, chymotrypsin, factor Xa, factor XIa, and plasmin with SI higher than 10, while rRmS-17 inhibited trypsin, cathepsin G, chymotrypsin, plasmin, and factor XIa with SI of 1.6, 2.6, 2.7, 3.4, and 9.0, respectively. Additionally, we observed the formation of irreversible complexes between rRmS-3 and chymotrypsin, rRmS-6/rRmS-17 and trypsin, and rRmS-3/rRmS-17 and cathepsin G, which is consistent with typical mechanism of inhibitory serpins. In blood clotting assays, rRmS-17 delayed plasma clotting by 60 s in recalcification time assay, while rRmS-3 and rRmS-6 did not have any effect. Consistent with inhibitor function profiling data, 2.0 µM rRmS-3 and rRmS-17 inhibited cathepsin G-activated platelet aggregation in a dose-responsive manner by up to 96% and 95% respectively. Of significant interest, polyclonal antibodies blocked inhibitory functions of the three serpins. Also notable, antibodies to Amblyomma americanum, Ixodes scapularis, and Rhipicephalus sanguineus tick saliva proteins cross-reacted with the three R. microplus saliva serpins, suggesting the potential of these proteins as candidates for universal anti-tick vaccines.

Heterogeneity in perceptual category learning by high functioning children with autism spectrum disorder.

Previous research suggests that high functioning (HF) children with autism spectrum disorder (ASD) sometimes have problems learning categories, but often appear to perform normally in categorization tasks. The deficits that individuals with ASD show when learning categories have been attributed to executive dysfunction, general deficits in implicit learning, atypical cognitive strategies, or abnormal perceptual biases and abilities. Several of these psychological explanations for category learning deficits have been associated with neural abnormalities such as cortical underconnectivity. The present study evaluated how well existing neurally based theories account for atypical perceptual category learning shown by HF children with ASD across multiple category learning tasks involving novel, abstract shapes. Consistent with earlier results, children's performances revealed two distinct patterns of learning and generalization associated with ASD: one was indistinguishable from performance in typically developing children; the other revealed dramatic impairments. These two patterns were evident regardless of training regimen or stimulus set. Surprisingly, some children with ASD showed both patterns. Simulations of perceptual category learning could account for the two observed patterns in terms of differences in neural plasticity. However, no current psychological or neural theory adequately explains why a child with ASD might show such large fluctuations in category learning ability across training conditions or stimulus sets.

The interplay between uncertainty monitoring and working memory: Can metacognition become automatic?

The uncertainty response has grounded the study of metacognition in nonhuman animals. Recent research has explored the processes supporting uncertainty monitoring in monkeys. It has revealed that uncertainty responding, in contrast to perceptual responding, depends on significant working memory resources. The aim of the present study was to expand this research by examining whether uncertainty monitoring is also working memory demanding in humans. To explore this issue, human participants were tested with or without a cognitive load on a psychophysical discrimination task that included either an uncertainty response (allowing the participant to decline difficult trials) or a middle-perceptual response (labeling the same intermediate trial levels). The results demonstrated that cognitive load reduced uncertainty responding, but increased middle responding. However, this dissociation between uncertainty and middle responding was only observed when participants either lacked training or had very little training with the uncertainty response. If more training was provided, the effect of load was small. These results suggest that uncertainty responding is resource demanding, but with sufficient training, human participants can respond to uncertainty either by using minimal working memory resources or by effectively sharing resources. These results are discussed in relation to the literature on animal and human metacognition.

Kinetics of Leptospira interrogans infection in hamsters after intradermal and subcutaneous challenge.

BACKGROUND: Leptospirosis is a zoonosis caused by highly motile, helically shaped bacteria that penetrate the skin and mucous membranes through lesions or abrasions, and rapidly disseminate throughout the body. Although the intraperitoneal route of infection is widely used to experimentally inoculate hamsters, this challenge route does not represent a natural route of infection. METHODOLOGY/PRINCIPAL FINDINGS: Here we describe the kinetics of disease and infection in hamster model of leptospirosis after subcutaneous and intradermal inoculation of Leptospira interrogans serovar Copenhageni, strain Fiocruz L1-130. Histopathologic changes in and around the kidney, including glomerular and tubular damage and interstitial inflammatory changes, began on day 5, and preceded deterioration in renal function as measured by serum creatinine. Weight loss, hemoconcentration, increased absolute neutrophil counts (ANC) in the blood and hepatic dysfunction were first noted on day 6. Vascular endothelial growth factor, a serum marker of sepsis severity, became elevated during the later stages of infection. The burden of infection, as measured by quantitative PCR, was highest in the kidney and peaked on day 5 after intradermal challenge and on day 6 after subcutaneous challenge. Compared to subcutaneous challenge, intradermal challenge resulted in a lower burden of infection in both the kidney and liver on day 6, lower ANC and less weight loss on day 7. CONCLUSIONS/SIGNIFICANCE: The intradermal and subcutaneous challenge routes result in significant differences in the kinetics of dissemination and disease after challenge with L. interrogans serovar Copenhageni strain Fiocruz L1-130 at an experimental dose of 2×106 leptospires. These results provide new information regarding infection kinetics in the hamster model of leptospirosis.

Endodontic-related facial paresthesia: systematic review.

Paresthesia is a neurosensitivity disorder caused by injury to the neural tissue. It is characterized by a burning or twinging sensation or by partial loss of local sensitivity. Paresthesia related to endodontic treatment can occur because of extravasation of filling material or the intracanal dressing, as a consequence of periapical surgery or because of periapical infection. A literature review of paresthesia in endodontics was undertaken, with a view to identifying and discussing the most commonly affected nerves, the diagnostic process and the treatment options. Among reported cases, the most commonly affected nerves were those passing through the jaw: the inferior alveolar nerve, the mental nerve and the lingual nerve. To diagnose paresthesia, the endodontist must carry out a complete medical history, panoramic and periapical radiography, and (in some cases) computed tomography, as well as mechanoceptive and nociceptive tests. To date, no specific treatment for endodontic-related paresthesia has been described in the literature, since the problem may be related to a variety of causes.

Decision deadlines and uncertainty monitoring: the effect of time constraints on uncertainty and perceptual responses.

The behavioral uncertainty response has grounded the study of animal metacognition and influenced the study of human psychophysics. However, the interpretation of this response is debated--especially whether it is a behavioral index of metacognition. The authors advanced this interpretation using the dissociative technique of response deadlines. Uncertainty responding, if it is higher level or metacognitive, should depend on a slower, more controlled decisional process and be more vulnerable to time constraints. Humans performed sparse-uncertain-dense or sparse-middle-dense discriminations in which, respectively, they could decline difficult trials or positively identify middle stimuli. Uncertainty responses were sharply and selectively reduced under a decision deadline, as compared to primary perceptual responses (i.e., "sparse," "middle," and "dense" responses). This dissociation suggests that the uncertainty response does reflect a higher-level, decisional response. It grants the uncertainty response a distinctive psychological role in its task and encourages an interpretation of this response as an elemental behavioral index of uncertainty that deserves continuing research.

Tissue expression and the host's immunological recognition of a Rhipicephalus microplus paramyosin.

Rhipicephalus microplus is a parasite that causes economic losses in cattle herds, and immunological control is the most promising alternative to replace chemical control. The muscular protein paramyosin has been additionally found in non-muscle tissues and characterized as presenting activities that enable the evasion of the host's immune system in various parasites. This report investigated the recognition level of paramyosin by sera of infested bovines, its expression in tissues, organs and different life stages of R. microplus. ELISA analyses showed that paramyosin and salivary gland extract were recognized by infested Bos taurus and B. indicus sera. Paramyosin gene expression was evaluated in egg, larvae, adult male, and several tissues of partially- and fully-engorged females by qRT-PCR, showing the highest expression levels in fat body. These results show that R. microplus paramyosin is immunologically recognized during the tick infestation and together with the high transcription rate found in organs that do not present a highly developed musculature, further suggests that it may possess additional, non-muscle functions in the tick-bovine relationship.