Participation of osteoclastogenic factors in immunopathogenesis of human chronic periapical lesions.

BACKGROUND: Chronic periapical lesions (CPLs) are common lesions of the oral cavity and are the result of caries, tooth fracture, iatrogenic causes, or factors causing contamination and pulp necrosis. Inflammatory cells participate in the expansion of CPLs by releasing factors that stimulate or inhibit osteolytic activity. The objective of this study was to investigate the participation of RANKL, TNF-α, cathepsin K, IL-33, and OPG in the development of radicular cysts (RCs) and periapical granulomas (PGs). METHODS: Paraffin-embedded sections of 30 RCs and 22 PGs were submitted to immunohistochemistry. RESULTS: Immunoexpression of the proteins studied was observed in the epithelium and capsule of RCs, as well as in connective tissue of PGs. The expression of the osteoclastogenic factors studied differed significantly in RCs and PGs (P < .001), with lower expression of OPG in RCs. In PGs, the lowest expression was observed for cathepsin K. Comparison of the 2 lesions showed a similar participation of RANKL and IL33, while a significant difference was observed for OPG (P < .001), TNF-α (P = .002), and cathepsin K (P = .016). No association of the expression of the proteins with lesions size was observed. CONCLUSIONS: This study demonstrated the participation of RANKL, TNF-α, IL-33, cathepsin K, and OPG in the development of RCs and PGs, with emphasis on the highest immunoreactivity of cathepsin in RCs and TNF-α and OPG in PGs. OPG possibly determines the slower growth of PGs compared to RCs.

Honey bee attractants and pollination in sweet orange, Citrus sinensis (L.) Osbeck, Var. Pera-rio

This experiment studied the frequency and behavior of insects on sweet orange flowers, Citrus sinensis (L.) Osbeck, their effect on fruit production (quantity and quality), nectar and pollen collection

[Anatomo-clinical diagnosis correlation. Retrospective assessment of the clinical diagnosis in necropsies]. / Correlação diagnóstica anatomoclínica. Aferição retrospectiva do diagnóstico clínico em necrópsias.

The post mortem findings in 200 autopsies were compared with the clinical diagnoses. Twenty diseases were analysed with respect to clinico-pathological correlation. In relation to the respective totals the diseases most frequently missed clinically were (false negative) pyelonephritis (100%), pulmonary embolus (87.50%) and bronchopneumonia (58.16%). In relation to the respective totals the clinical diagnoses less frequently confirmed (false positive) were tuberculosis (69.56%), paracoccidioidomycosis (57.14%), sepsis (53.13%) and Chagas' disease (44.44%). There was clinicopathological agreement in 97 autopsies (48.50%). In 19 cases (9.50%) if the diagnostic error had been detected during life this probably would have changes the prognosis. The findings are discussed in the light of previous studies. The importance of routine post-mortem examination and clinico-pathological correlation is stressed.