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1.
Harmful Algae ; 78: 1-8, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30196917

RESUMO

Three strains of the toxic benthic dinoflagellate Prorocentrum hoffmannianum were isolated in the Canary Islands (north-east Atlantic Ocean, Spain). The identity of the strains was determined by phylogenetic analyses of partial LSU rDNA (D1-D2 regions) but their morphology based on SEM images corresponded to P. maculosum. Their toxin profiles were analyzed by liquid chromatography and high resolution mass spectrometry analysis (LC-HRMS) on cell extracts and culture media. Okadaic acid and three analogs were detected in all strains. Rather, in culture media the detected compounds were variable among strains, two of them being okadaic acid analogs not found on cell extracts. As a result, the taxonomy of the species was revised and P. maculosum is proposed as a junior synonym of P. hoffmannianum whose description is emended.


Assuntos
Dinoflagellida/química , Dinoflagellida/classificação , Toxinas Marinhas/química , Ácido Okadáico/química , Cromatografia Líquida , Dinoflagellida/citologia , Dinoflagellida/genética , Espectrometria de Massas , Filogenia , RNA de Algas/análise , RNA de Protozoário/análise , RNA Ribossômico/análise , Espanha
2.
Mar Drugs ; 12(1): 176-92, 2014 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-24402177

RESUMO

Marine organisms are an increasingly important source of novel metabolites, some of which have already inspired or become new drugs. In addition, many of these molecules show a high degree of novelty from a structural and/or pharmacological point of view. Structure determination is generally achieved by the use of a variety of spectroscopic methods, among which NMR (nuclear magnetic resonance) plays a major role and determination of the stereochemical relationships within every new molecule is generally the most challenging part in structural determination. In this communication, we have chosen okadaic acid as a model compound to perform a computational chemistry study to predict 1H and 13C NMR chemical shifts. The effect of two different solvents and conformation on the ability of DFT (density functional theory) calculations to predict the correct stereoisomer has been studied.


Assuntos
Produtos Biológicos/química , Ácido Okadáico/química , Algoritmos , Animais , Biologia Computacional , Cristalografia por Raios X , Dinoflagellida , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Conformação Molecular , Teoria Quântica , Solventes , Estereoisomerismo
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