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1.
Methods Mol Biol ; 566: 109-21, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-20058168

RESUMO

Posttranslational modifications such as phosphorylation and ubiquitination serve, independently or together, as gatekeepers of protein transport and turnover in normal and disease physiologies. Aberrant protein phosphorylation is one of the defining pathological hallmarks of more than 20 different neurodegenerative disorders, including Alzheimer's disease (AD). The disruption of the phosphorylation of neurotransmitter receptors has been implicated as one of the causal factors of impaired memory function in AD (1-3). Another feature of AD is the aberrant accumulation of proteins that are normally degraded by the ubiquitin proteasome system upon being conjugated to ubiquitin. Thus, elucidating the protein targets of phosphorylation and ubiquitination that can serve as AD biomarkers will aid in the development of effective therapeutic approaches to the treatment of AD. This chapter provides details pertaining to the qualitative and quantitative liquid chromatography tandem mass spectrometry-based analysis of an affinity purified, phosphorylated, and ubiquitinated protein, paired-helical filament tau.


Assuntos
Doença de Alzheimer/fisiopatologia , Processamento de Proteína Pós-Traducional , Proteoma/análise , Proteínas tau , Doença de Alzheimer/patologia , Cromatografia Líquida/métodos , Eletroforese em Gel de Poliacrilamida/métodos , Humanos , Espectrometria de Massas/métodos , Fosforilação , Ubiquitinação , Proteínas tau/química , Proteínas tau/metabolismo
2.
J Proteome Res ; 7(7): 2803-11, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18549264

RESUMO

Phosphopeptide identification and phosphorylation site localization are crucial aspects of many biological studies. Furthermore, multiple phosphorylations of peptides make site localization even more difficult. We developed a probability-based method to unambiguously determine phosphorylation sites within phosphopeptides using MS2/3 pair information. A comparison test was performed with SEQUEST and MASCOT predictions using a spectral data set from a synthetic doubly phosphorylated peptide, and the results showed that PhosphoScan analysis yielded a 63% phosphopeptide localization improvement compared with SEQUEST and a 57% improvement compared with MASCOT.


Assuntos
Fosfopeptídeos/análise , Doença de Alzheimer/metabolismo , Encéfalo/metabolismo , Cromatografia Líquida , Bases de Dados Factuais , Humanos , Fosforilação , Probabilidade , Processamento de Proteína Pós-Traducional , Espectrometria de Massas em Tandem , Proteínas tau/metabolismo
3.
Biochemistry ; 46(45): 13031-40, 2007 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-17944490

RESUMO

Both metal and flavin-dependent sulfhydryl oxidases catalyze the net generation of disulfide bonds with the reduction of oxygen to hydrogen peroxide. The first mammalian sulfhydryl oxidase to be described was an iron-dependent enzyme isolated from bovine milk whey (Janolino, V.G., and Swaisgood, H.E. (1975) J. Biol. Chem. 250, 2532-2537). This protein was reported to contain 0.5 atoms of iron per 89 kDa subunit and to be completely inhibited by ethylenediaminetetraacetate (EDTA). However the present work shows that a soluble 62 kDa FAD-linked and EDTA-insensitive sulfhydryl oxidase apparently constitutes the dominant disulfide bond-generating activity in skim milk. Unlike the metalloenzyme, the flavoprotein is not associated tightly with skim milk membranes. Sequencing of the purified bovine enzyme (>70% coverage) showed it to be a member of the Quiescin-sulfhydryl oxidase (QSOX) family. Consistent with its solubility, this bovine QSOX1 paralogue lacks the C-terminal transmembrane span of the long form of these proteins. Bovine milk QSOX1 is highly active toward reduced RNase and with the model substrate dithiothreitol. The significance of these new findings is discussed in relation to the earlier reports of metal-dependent sulfhydryl oxidases.


Assuntos
Leite/enzimologia , Oxirredutases/metabolismo , Sequência de Aminoácidos , Animais , Bovinos , Galinhas , Cromatografia em Gel , Clara de Ovo/análise , Feminino , Dados de Sequência Molecular , Oxirredutases/isolamento & purificação , Alinhamento de Sequência
4.
J Biol Chem ; 281(16): 10825-38, 2006 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-16443603

RESUMO

One of the key pathological hallmarks of Alzheimer disease (AD) is the accumulation of paired helical filaments (PHFs) of hyperphosphorylated microtubule-associated protein Tau. Tandem mass spectrometry was employed to examine PHF-Tau post-translational modifications, in particular protein phosphorylation and ubiquitination, to shed light on their role in the early stages of Alzheimer disease. PHF-Tau from Alzheimer disease brain was affinity-purified by MC1 monoclonal antibody to isolate a soluble fraction of PHF-Tau in a conformation unique to human AD brain. A large number of phosphorylation sites were identified by employing a data-dependent neutral loss algorithm to trigger MS3 scans of phosphopeptides. It was found that soluble PHF-Tau is ubiquitinated at its microtubule-binding domain at residues Lys-254, Lys-311, and Lys-353, suggesting that ubiquitination of PHF-Tau may be an earlier pathological event than previously thought and that ubiquitination could play a regulatory role in modulating the integrity of microtubules during the course of AD. Tandem mass spectrometry data for ubiquitin itself indicate that PHF-Tau is modified by three polyubiquitin linkages, at Lys-6, Lys-11, and Lys-48. Relative quantitative analysis indicates that Lys-48-linked polyubiquitination is the primary form of polyubiquitination with a minor portion of ubiquitin linked at Lys-6 and Lys-11. Because modification by Lys-48-linked polyubiquitin chains is known to serve as the essential means of targeting proteins for degradation by the ubiquitin-proteasome system, and it has been reported that modification at Lys-6 inhibits ubiquitin-dependent protein degradation, a failure of the ubiquitin-proteasome system could play a role in initiating the formation of degradation-resistant PHF tangles.


Assuntos
Doença de Alzheimer/metabolismo , Lisina/química , Ubiquitina/química , Proteínas tau/química , Algoritmos , Sequência de Aminoácidos , Cromatografia Líquida , Humanos , Immunoblotting , Imunoprecipitação , Espectrometria de Massas , Dados de Sequência Molecular , Degeneração Neural/metabolismo , Emaranhados Neurofibrilares/metabolismo , Peptídeos/química , Fosforilação , Complexo de Endopeptidases do Proteassoma/metabolismo , Conformação Proteica , Estrutura Terciária de Proteína , Homologia de Sequência de Aminoácidos , Serina/química , Solventes/química , Treonina/química , Tripsina/química
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