RESUMO
The nutritional composition of bovine liver, particularly in terms of vitamins E, ß-carotene, and cholesterol concentration, is significantly influenced by the cattle-rearing system and diet. This study aimed to elucidate the impact of four predominant cattle-rearing systems in the Eastern Amazon region on the vitamin E, ß-carotene content, and cholesterol levels in bovine liver during the rainy season. Liver-tissue samples were collected from 48 cattle, with twelve specimens representing each rearing system. The systems encompassed two native pastures located in flood-prone areas, cultivated pastures on upland terrain and a confinement-rearing system. Our findings indicate that, when considering all rearing systems collectively, there were no significant differences in the levels of vitamins and cholesterol in the liver tissues (p > 0.05). However, a marked disparity in cholesterol levels emerged when comparing intensive rearing systems to extensive ones, with the former demonstrating notably higher concentrations (p = 0.01). Additionally, the intensive rearing system was associated with elevated levels of ß-carotene (p < 0.01), α-tocopherol (p = 0.01), and ß-tocopherol (p = 0.01) relative to the extensive systems. No significant variations were observed amongst the different extensive systems (p > 0.05). These results suggest that, while all rearing systems yielded liver tissues with typical concentrations of vitamins and cholesterol, the intensive rearing system led to a higher accumulation of certain vitamin compounds. This study highlights the substantial nutritional implications of different cattle-rearing systems in the Eastern Amazon and provides valuable insights for developing dietary strategies to optimize the nutritional quality of bovine liver. Therefore, the generated results are groundbreaking in the Eastern Amazon, Brazil, and inspire the development of new research projects to address other demands in this field and achieve additional outcomes.
RESUMO
O objetivo deste trabalho foi detectar a presença de DNA do Vírus da Imunodeficiência Felina (FIV) em gatos domesticos (Feliz catus) assintomáticos. Foi realizada a tecnica de reação em cadeia da polimerase (PCR) em 50 animais. Para tal, foram coletadas amostras de sangue, por venopunção da jugular, de forma asséptica para armazenamento de 1-2 mL de sangue total. Os animais que participaram do estudo fizeram parte do projeto de castração "Vida digna" da Universidade Federal Rural da Amazônia. E a escolha dos animais foi realizada de maneira aleatória, sem distinção por sexo ou idade, resultando em 29 foram fêmeas e 21 machos. Para o diagnóstico, foi realizada a extração do DNA, em seguida as amostras foram testadas em duas reações de PCR utilizando- se dois conjuntos de primers do Gene gag de FIV. Achou-se uma prevalência de 2% (1/50), confirmando assim a presença do vírus na cidade de Belém. Assim, evidenciando a importância de testar os felinos mesmo sendo assintomáticos. Desta forma, faz-se necessário a realização de trabalhos futuros que amplie o número amostral dos animais testados para assim elucidar o perfil epidemiológico da doença na região de Belém do Pará, considerando a relevância clínica desta infecção e a correta conduta médica veterinária para evitar novas infecções.
The objective of this work was to detect the presence of Feline Immunodeficiency Virus (FIV) proviral DNA in asymptomatic domestic cats (Feliz catus). The polymerase chain reaction technique was performed from 50 animals. For this, blood samples were collected by jugular venipuncture, aseptically for storage of 1-2 mL of whole blood. The animals that participated in the study were part of the castration project "Vida digna" at the Universidade Federal Rural da Amazônia. And the choice of animals was performed randomly, without distinction by sex or age, resulting in 29 females and 21 males. For diagnosis, DNA extraction was performed, then the samples were tested in two PCR reactions using two sets of FIV gag gene primers. A prevalence of 2% (1/50) was observed, thus confirming the presence of the virus in the city of Belém. Thus, highlighting the importance of testing the felines even if they are asymptomatic. Therefore, it is necessary to carry out future work that expands the sample number of animals tested in order to elucidate the epidemiological profile of the disease in the region of Belém do Pará, considering the clinical relevance of this infection and the correct veterinary medical conduct to avoid new infections.