[Report of Mycobacterium avium-intracellulare associated with kidney mycobacteriosis]. / Reporte de Mycobacterium avium-intracellulare asociado a micobacteriosis renal.

Consecutive samples of urine from a patient presenting radiological images of multicystic kidneys were microbiologically analyzed searching the possible presence of acid-alcohol resistant bacilli. The patient had already undergone complementary tests, which evidenced a kidney failure. As a result, it was isolated a slow-growth non pigmented mycobacterial strain corresponding to group III of Runyon. It was classified as Mycobacterium avium-intracellulare, according to conventional and alternative bacteriological studies, such as the analysis of fractions of mycobacterial mycolic acids by thin layer chromatography technique.

[Disseminated mycobacteriosis associated with Mycobacterium avium-intracellular (MAI) complex in HIV infected patients]. / Micobacteriosis diseminada asociada al complejo Mycobacterium avium-intracellulare (MAI), en paciente infectado con el virus de inmunodeficiencia humana.

Different types of samples from a 27-year-old female patient who was diagnosed AIDS after presenting an acute respiratory picture caused by Pneumocistis carinii, in 1997, were studied. From that moment on, the patient continued having respiratory pictures repeatedly, until she was admitted at "Pedro Kouri" Institute of Tropical Medicine with a chronic diarrheical picture, significant loss of weight and extreme anorexia, together with a constant wet cough, fever, etc. The necessary complementary tests were made and a hyperergic Mantoux and very low levels of CD4 cells (under 200 cells/m3) outstood. Microbiological studies were also conducted with samples from sputum, hemocultures and cerebrospinal fluid to discard the presence of acid-alcohol resistant bacilli (AARB). As a result of these studies, the repeated isolation was obtained in all types of worked samples from a slow-growth, creamy and non pigmented strain corresponding to Runyon's group III, which was classified as from the Mycobacterium avium-intracellulare complex. The patient was treated with antibacillary drugs without results, since she dies 4 weeks later due to the total dissemination of this infection. It should be stressed that it is the first case reported and microbiologically diagnosed of a generalized mycobacteriosis in a HIV positive patient due to Mycobacterium avium-intracellulare.

Micobacteriosis por Mycobacterium gordonae. Primer caso reportado en Cuba / Mycobacteriosis caused by Mycobacterium gordonae. First case reported in Cuba

Since 1990, repeated samples of a positive HIV patient were studied, who had skin lesions with ulcers and scabs, dirty bottom and prominent edges; hyperergic Mantoux and very low CD levels. As a result, a slowly growing pigmented strain was isolated and classified as Mycobacterium gordonae by using standard as well as alternative bacteriological studies such as the mycobacterial mycolic acid analysis. The patient was treated with 4 antibacilar drugs and 6 weeks after the starting of the treatment, all reported skin lesions had disappeared.

Análisis de ácidos grasos de cepas de Mycobacterium habana y Mycobacterium simiae / Analysis of fatty acids from strains of Mycobacterium habana and Mycobacterium simiae

A comparative analysis of mycobacterial fatty acid fractions of Mycobacteriun habana and Mycobacterium sineae strains was made. This study used the gas-liquid chromatography coupled with mass spectrometry. Chromatographic profiles obtained from this technique were exposed and compared. This technique proves to be valuable as an alternative element in mycobacterial characterization and makes it possible to analyze the possible differences that may exist among mycobacterial species and to identify the present fatty acid fractions. The outcome proved that the studied strains had quantifiable quantities of over 20 C atom chain fatty acids. There are small differences among the strains in terms of these organic components. It was confirmed that each of then describes a characteristic chromatographic pattern although the composition of present fatty acids is very similar in both studied species.