Oral Microbial Shift: Factors affecting the Microbiome and Prevention of Oral Disease.

BACKGROUND: Recently, oral microbiome has gained popularity among scientists. Microorganisms are no longer considered as disease-producing pathogens, rather they are now considered as partners of human in maintaining health. Since ancient times, changes in our lifestyle have affected our microbiome and the balance with their human host has been perturbed. The present review includes the description about factors affecting oral microbiome and establishing symbiosis with the human host so that they contribute in maintaining health rather than eliciting diseases. MATERIALS AND METHODS: A comprehensive literature search was performed on databases such as Google Scholar, PubMed and Medline until April 2015. First, articles were selected on the basis of their titles and then abstracts were screened and unwanted articles were excluded. Articles obtained from all the databases were checked and duplicate articles were removed. Articles obtained from various databases: PubMed = 35, Google Scholar=8. Out of these 43 articles, total 29 articles were finally selected for this review. RESULTS: The published literature suggests that the modern oral microbiome is less biodiverse, and possess more pathogenic bacterial species and lesser beneficial bacteria. The possible factors mainly responsible for this shift in microbiome were found to be change in diet, industrial revolution and indiscriminate use of antibiotics. CONCLUSION: Various changes in lifestyles have affected oral microbiome adversely and perturb the symbiosis between the microbiome and their hosts. The present oral microbiome is found to be less diverse and more pathogenic. The present review may be helpful in understanding the relationship between the microbiome and their human hosts so that microbiome contributes in maintaining healthy state of the body.

Effect of Various Sugary Beverages on Salivary pH, Flow Rate, and Oral Clearance Rate amongst Adults.

Introduction. Diet is a major aetiological factor for dental caries and enamel erosion. This study was undertaken with the aim of assessing the effect of selected locally available beverages on salivary pH, flow rate, and oral clearance rate amongst adults. Materials and Method. This clinical trial comprised 120 subjects. Test beverages undertaken were pepsi, fruit drink, coffee, and sweetened milk. Statistical analysis was carried out using SPSS version 17. Descriptive statistics, one-way ANOVA, and post hoc Tukey's test were applied in the statistical tests. Results. It was found that salivary pH decreased for all the beverages immediately after consumption and the salivary flow rate increased after their consumption. The oral clearance rate of sweetened milk was found to be the least at 6.5 minutes and that of pepsi was found to be 13 minutes. However, the oral clearance rates of fruit drink and coffee were found to be equal at 15 minutes. Conclusion. Although it was found out that liquids cleared rapidly from the oral cavity, they had a significant cariogenic and erosive potential. Hence, it is always advised to minimise the consumption of beverages, especially amongst children and young adults to maintain a good oral health.

Essential oils, their therapeutic properties, and implication in dentistry: A review.

BACKGROUND: Antibacterial treatments currently used for treatment cause several side effects, and bacterial resistance to the antibiotics is also increasing. Therefore, there is need to find better alternatives. Essential oils (EOs) have been used for treatment of various ailments since ancient times and have gained popularity over the years. Safety and efficacy of EOs have been proved by several clinical trials. This review gives an overview on the EOs, their uses, and adverse effects. MATERIALS AND METHODS: A literature search was performed in the PubMed for clinical trial studies and review articles on EOs published up to February 2015. The search was performed during March 2015. The following keywords were used: "Lavender essential oil," "cinnamon oil," "clove oil," "eucalyptus oil," "peppermint oil," "lemon EOs," and "tea tree oil." RESULTS: Total 70 relevant articles were found in PubMed database. After screening of abstracts, 52 articles were selected to be included in the present review. CONCLUSION: On the basis of the available information, it can be concluded that EOs have the potential to be developed as preventive or therapeutic agents for various oral diseases, but further clinical trials are required to establish their safety and efficacy.

Utilization of Dental Services in Public Health Center: Dental Attendance, Awareness and Felt Needs.

BACKGROUND: In rural India, dental diseases occur due to many factors, which includes inadequate or improper use of fluoride and a lack of knowledge regarding oral health and oral hygiene, which prevent proper screening and dental care of oral diseases. The objective of the study was to evaluate the dental attendance, awareness and utilization of dental services in public health center. MATERIALS AND METHODS: A cross-sectional study was conducted among 251 study subjects who were visiting dental outpatient department (OPD) of public health centre (PHC), Guda Bishnoi, and Jodhpur using a pretested proforma from month of July 2014 to October 2014. A pretested questionnaire was used to collect the data regarding socioeconomic status and demographic factors affecting the utilization of dental services. Pearson's Chi-square test and step-wise logistic regression were applied for the analysis. RESULTS: Statistically significant results were found in relation to age, educational status, socioeconomic status and gender with dental attendance, dental awareness and felt needs. p-value <0.05 was kept as statistically significant. CONCLUSION: The services provided in public health center should be based on the felt need of the population to increase attendance as well as utilization of dental services, thereby increasing the oral health status of the population.

Oral Paleomicrobiology: Study of Ancient Oral Microbiome.

BACKGROUND: Paleomicrobiology is a special branch of micropaleontology concerned with the study of bacterial fossils. We have used the term 'oral paleomicrobiology', as in this review we have focused on the ancient oral microflora. Recently, dental calculus and dental pulp have been identified as rich sources of ancient microbial DNA. Study of this ancient genetic material opens a new door to the ancient world. This review gives an overview of history of ancient DNA research, various techniques of analyzing ancient DNA in dental calculus and dental pulp, and the implications of the oral paleomicrobiology. MATERIALS AND METHODS: A comprehensive literature search was performed in the following databases-pubmed, medline and google scholar for studies published before 10 April, 2015. The following keywords were used- 'ancient DNA', 'ancient oral flora, 'oral paleomicrobiology' and 'oral microbiome', '16S rRNA sequencing'. To obtain additional data, a manual search was performed using the reference lists of selected articles. RESULT: As a result of literature search, 27 articles were found in pubmed, 12 in google scholar and one in medline. Eight more articles were selected from the reference list of selected articles. CONCLUSION: The combination of microbiology and paleontology has brought a revolution in the study of human evolution and microbial communities. The naturally well-preserved samples of microbial DNA from dental pulp and microbial colonies trapped in dental calculus are a potential source of microbial genetic material, which will prove invaluable in resolving mysteries of the past. This may be a beginning of a new era of oral paleomicrobiology, which will contribute in our studies about prevention of disease by establishing symbiosis between human beings and their microbiome.

Occurrence of domestic violence among women and its impact on oral health in Jodhpur City.

BACKGROUND: This study had a two objectives to determine the prevalence of domestic violence and to know the impact of the same on the oral health. MATERIALS AND METHODS: An observational cross-sectional study was done in Jodhpur, Rajasthan; among 150 married women of 18 to 60 years of age using a predesigned pretested proforma from 18th January 2014 to 27th February 2014. Pretested semistructured open-ended questionnaire used for collecting the data from the study subjects. Pearson's Chi-square test and p-value were used to calculated the occurrence and impact of domestic violence on oral health among women. RESULTS: The most common form of domestic violence found was physical abuse (54.7%) followed by emotional abuse (20.0%) and financial abuse (14.0%). The most important risk factor was alcoholism followed by literacy status and having a girl child. Study shows that physical abuse is more in graduate than lower level of educational females. Females living in urban area show highest frequency of physical abuse than those living in rural area. Injury to the face were highly reported (38.7%) followed by injury to lip (13.3%) and nose fracture/bleeding nose (9.3%). CONCLUSION: Government has to take stringent action to prevent domestic violence by making women more self-reliant especially by making women more literate and more financially independent.

Oral health knowledge, attitude and practices of children and adolescents of orphanages in jodhpur city rajasthan, India.

OBJECTIVES: This study had twin objectives of assessing the oral health knowledge, attitude and practices and to assess the dental caries status and treatment needs among the orphan children of orphanages of Jodhpur city, Rajasthan, India. MATERIALS AND METHODS: This cross- sectional study was carried out on 100 children to assess the oral health knowledge, attitude and practices of children and adolescents of orphanages in Jodhpur city, Rajasthan, India. The data was collected on a pre-tested questionnaire which included 20 closed ended multiple-choice questions on perceived oral health status, knowledge of oral health and attitude, oral health practices, dietary habits and behaviour towards dental treatment. On completion of the questionnaire, each child underwent an oral examination and Dentition status and treatment needs index (WHO Oral Health Surveys- 1997) was recorded for each subject. RESULTS: Almost 93% of the children felt the necessity of maintaining oral hygiene. There were 69% of the children who believed that it was necessary to brush teeth after every meal, 51% children believed that regular tooth-brushing prevents all tooth problems and 93% children knew that tobacco is carcinogenic in nature. Also, it was found that 77% of the children believed that regular dental visits help in maintaining oral hygiene. CONCLUSION: Many of them had acquired knowledge on oral health. More than half of the study subjects were aware of the importance of keeping good oral hygiene, regular dental visits and harmful effects of tobacco.

Effect of tetracycline HCl in the treatment of chronic periodontitis - A clinical study.

OBJECTIVE: This study was aimed to evaluate the efficacy of the adjunctive use of tetracycline fibers (Periodontal Plus AB(®)) as a local drug delivery with scaling and root planing, as compared with the results of one episode of scaling and root planing for the treatment of chronic periodontitis. MATERIALS AND METHODS: The effectiveness of Periodontal Plus AB (tetracycline fiber) was assessed in 100 patients suffering from chronic periodontitis using split-mouth technique. STATISTICAL ANALYSIS: The relative efficacy of the two treatment modalities was evaluated using the paired Student's t-test, and the comparative evaluation between the two groups was done using the independent Student's t-test. RESULTS: Significant improvement was found in all the variables, including reduction in pocket depth and gain in clinical attachment level, in both test and control groups in 3 months, which was statistically significant. Mean reduction in pocket depth and gain in clinical attachment level were more in test than in control group. CONCLUSION: Tetracycline fiber therapy along with scaling and root planing improves the healing outcome, namely, reduction in pocket depth and gain in clinical attachment level, when compared to scaling and root planing alone.

Possible use of essential oils in dentistry.

How to cite the article: Dagli N, Dagli R. Possible use of essential oils in dentistry. J Int Oral Health 2014;6(3):i-ii.

Is Alcohol an independent risk factor for Oro-Pharyngeal and Pulmonary Carcinogenesis - An Acetaldehyde concentrations based Double Blinded Randomized Control Trial.

BACKGROUND: There is increasing evidence that a major part of the tumour-promoting action of alcohol is mediated via its first, toxic and carcinogenic metabolite acetaldehyde. MATERIALS & METHODS: The double blinded randomized control trial was designed for 82 male volunteers aged 20-29 years. Exclusion criteria were individual under antibiotic therapy, smokers, mutant Aldehyde Dehydrogenase deficient subject or any other systemic disease. Subjects were randomized in experimental (alcohol + soft drink) and control group (soft drink) from each pair of equal body weighted volunteers. The amount of alcohol consumed was calculated to be equivalent to 0.7 g alcohol/kilogram of body weight. Samples of breath for Acetaldehyde concentration (AC) were captured with the aid of a highly reproducible fuel cell gas-sampling device (PST-M1; Lions Laboratories, Cardiff, Wales). In Statistical analysis, mean AC was compared among both groups at different interval using paired t-test and Analysis of variance. RESULTS: Mean acetaldehyde level was recorded higher ([Formula: see text]) among interventional group which can be produced from ethanol during metabolism or by oro-pharyngeal microbes. After 15 minutes of drink, the AC was [Formula: see text] in ethanol group compared to [Formula: see text] in soft-drink group. There was significant increase in AC after 1 hour ([Formula: see text]) which was [Formula: see text] in ethanol group compared to [Formula: see text] in soft-drink group. CONCLUSION: Although acetaldehyde is metabolite of alcohol, its organ specific production with risk for oro-pharyngeal and pulmonary carcinogenesis makes alcohol an independent risk factor of carcinogenesis. How to cite this article: Dagli RJ, Kulkarni S, Duraiswamy P, Dagli NR, Khara NV, Khara BN. Is Alcohol an independent risk factor for Oro-Pharyngeal and Pulmonary Carcinogenesis - An Acetaldehyde concentrations based Double Blinded Randomized Control Trial. J Int Oral Health 2013; 5(4):62-67.

Relationship of body mass index with periodontal health status of green marble mine laborers in Kesariyaji, India.

It is evident from literature that an increased body mass index (BMI) may be a potential risk factor for periodontitis. Association between BMI and periodontitis has been ascribed to unhealthy dietary patterns with insufficient micronutrients and excess sugar and fat content. The present study population has been plagued by unhealthy nutritional practices, hence the present study intended to assess the relation between BMI and periodontal status among green marble mine laborers of Kesariyaji, in the Udaipur district of Rajasthan, India. The study sample comprised of 513 subjects aged 18-54 years, drawn using the stratified cluster sampling procedure. BMI was calculated as the ratio of the subject's body weight (in kg) to the square of their height (in meters). Periodontal status was recorded using the Community Periodontal Index (CPI). Binary multiple logistic regression analysis was executed to assess the relation between body mass index and periodontitis. The dependent variable for logistic regression analysis was categorized into control group (scores 0 - 2 of the CPI) and periodontitis group (scores 3 and 4 of the CPI). The overall prevalence of periodontal disease was 98.2%. Caries status and mean number of teeth present deteriorated with the poor periodontal status. Subjects had an increased risk of periodontitis by 57% for each 1kg/m(2) increase in the body mass index, which means that a higher body mass index could be a potential risk factor for periodontitis among the adults aged 18 to 54 years. In conclusion, evaluation of the body mass index could be used in periodontal risk assessment.

Relationship of Body Mass Index with periodontal health status of green marble mine laborers in Kesariyaji, India

It is evident from literature that an increased body mass index (BMI) may be a potential risk factor for periodontitis. Association between BMI and periodontitis has been ascribed to unhealthy dietary patterns with insufficient micronutrients and excess sugar and fat content. The present study population has been plagued by unhealthy nutritional practices, hence the present study intended to assess the relation between BMI and periodontal status among green marble mine laborers of Kesariyaji, in the Udaipur district of Rajasthan, India. The study sample comprised of 513 subjects aged 18-54 years, drawn using the stratified cluster sampling procedure. BMI was calculated as the ratio of the subject's body weight (in kg) to the square of their height (in meters). Periodontal status was recorded using the Community Periodontal Index (CPI). Binary multiple logistic regression analysis was executed to assess the relation between body mass index and periodontitis. The dependent variable for logistic regression analysis was categorized into control group (scores 0 - 2 of the CPI) and periodontitis group (scores 3 and 4 of the CPI). The overall prevalence of periodontal disease was 98.2 percent. Caries status and mean number of teeth present deteriorated with the poor periodontal status. Subjects had an increased risk of periodontitis by 57 percent for each 1kg/m² increase in the body mass index, which means that a higher body mass index could be a potential risk factor for periodontitis among the adults aged 18 to 54 years. In conclusion, evaluation of the body mass index could be used in periodontal risk assessment.

Oral health status and practices of dentate Bhil adult tribes of southern Rajasthan, India.

AIMS: To assess the oral health status of the Bhil tribal population of Southern Rajasthan and to investigate the association of age, oral hygiene and dental visiting practices with oral health status. DESIGN: A cross sectional study of Bhil tribal adults chosen by a multi stage stratified random sampling procedure. PARTICIPANTS: The total sample size was 1590 male tribal dentate subjects aged 15-54 years. METHODS: Clinical recordings of oral hygiene status (OHI-S), caries status (DMFT and DMFS) and treatment needs, and periodontal status (CPI). The Chi square test was applied to discrete data and one way ANOVA for continuous data. Multivariate analyses were carried out to test the association of age, frequency of cleaning teeth, material used for cleaning teeth and dental visiting habits with caries and periodontal status. RESULTS: Debris, calculus and oral hygiene index scores increased with age. The overall mean DMFT and DMFS scores were 5.34 +/- 6.48 and 18.94 +/- 35.87 respectively. Extraction was the most required treatment (1.74 +/- 3.66 teeth) followed by one surface fillings (1.34 +/- 1.65 teeth). Shallow periodontal pockets were prevalent (40%) among the 35-44 years age group whereas deep pockets were most common (11.6%) in the oldest age group. More than half the sextants (3.15) were excluded amongst the oldest study group. All the independent variables namely age, frequency of cleaning teeth, substance used for cleaning teeth and visiting habits were statistically significantly related to caries and periodontal status. CONCLUSIONS: The study population was characterised by a lack of previous dental care, high treatment needs, high prevalence of periodontal disease and poor oral hygiene. Under these circumstances, the implementation of a basic oral health care programme for the Bhil population is a high priority.

Oral hygiene status in relation to sociodemographic factors of children and adults who are hearing impaired, attending a special school.

This study examined the oral hygiene levels and periodontal status in a group of children and adults with hearing impairment attending a special school in Udaipur, India. Oral hygiene status was assessed by the Simplified Oral Hygiene Index (OHI-S) of Greene and Vermillion and periodontal status by the Community Periodontal Index. An analysis using a bivariate analysis revealed that all the oral hygiene variables varied significantly with age, economic status, and education of the parents. A multiple regression analysis showed that the education of the mother was the single best predictor for oral hygiene status and explained 92% of the variance. These findings show that children with hearing impairment have poor oral hygiene and high levels of periodontal disease. This may be due to a lack of communication; hence, appropriate oral health education should be tailored to the needs of these students with the support of their teachers and their parents.

Dental caries experience and treatment needs of green marble mine laborers in Udaipur district, Rajasthan, India.

BACKGROUND AND OBJECTIVES: The study was undertaken at Kesariyaji, located in Udaipur district of Rajasthan. There are about 3 million workers who marble mine at Rajasthan. Living conditions of these workers are substandard and most of them are immigrant workers living in tiny shacks. Majority of them belong to lower socioeconomic status with poor educational background. The present study was carried out to estimate dental caries prevalence and treatment needs of laborers working in the green marble mines of Udaipur district. BASIC RESEARCH DESIGN: The data was collected using the methods and standards recommended by the WHO. Dentition status and treatment needs along with decayed, missing, and filled teeth (DMFT) index, and decayed, missing, and filled surfaces score were recorded. Standard error of mean was calculated for all the mean values of treatment needs. There were three examiners, who were trained before the survey for inter-examiner variability, and the reliability was tested by means of weighted kappa statistics, which was 90%. PARTICIPANTS: The study population comprised 513 men in four age groups of 18-25, 26-34, 35-44, and 45-54 years, respectively. RESULTS: The mean DMFT for all age groups was 3.13 with highest mean of 4.0 for the age group of 45-54 years. Mean decayed teeth were 2.60, 3.33, 1.46, and 1.5 for the age groups 15-24, 25-34, 35-44, and 45-54 years, respectively. Filled component was nil for all age groups. Most of the subjects required one surface filling with a very less proportion needing pulp care. CONCLUSIONS: The missing component constituted the major part of DMFT index in the 45-54 years age group and the absence of filled component in the whole study population implies that the treatment needs of the study population are unmet. Thus, intervention in the form of oral health promotion and curative services are the need of the hour.