Bone health and body composition in transgender adults before gender-affirming hormonal therapy: data from the COMET study.

PURPOSE: Preliminary data suggested that bone mineral density (BMD) in transgender adults before initiating gender-affirming hormone therapy (GAHT) is lower when compared to cisgender controls. In this study, we analyzed bone metabolism in a sample of transgender adults before GAHT, and its possible correlation with biochemical profile, body composition and lifestyle habits (i.e., tobacco smoke and physical activity). METHODS: Medical data, smoking habits, phospho-calcic and hormonal blood tests and densitometric parameters were collected in a sample of 125 transgender adults, 78 Assigned Females At Birth (AFAB) and 47 Assigned Males At Birth (AMAB) before GAHT initiation and 146 cisgender controls (57 females and 89 males) matched by sex assigned at birth and age. 55 transgender and 46 cisgender controls also underwent a complete body composition evaluation and assessment of physical activity using the International Physical Activity Questionnaire (IPAQ). RESULTS: 14.3% of transgender and 6.2% of cisgender sample, respectively, had z-score values < -2 (p = 0.04). We observed only lower vitamin D values in transgender sample regarding biochemical/hormonal profile. AFAB transgender people had more total fat mass, while AMAB transgender individuals had reduced total lean mass as compared to cisgender people (53.94 ± 7.74 vs 58.38 ± 6.91, p < 0.05). AFAB transgender adults were more likely to be active smokers and tend to spend more time indoor. Fat Mass Index (FMI) was correlated with lumbar and femur BMD both in transgender individuals, while no correlations were found between lean mass parameters and BMD in AMAB transgender people. CONCLUSIONS: Body composition and lifestyle factors could contribute to low BMD in transgender adults before GAHT.

Comparative analyses in Lotus: the cytogenetic map of Lotus uliginosus Schkuhr.

A comparative cytogenetic map was built for Lotusuliginosus (2n = 12), expanding previous analyses that revealed intra- and interspecific chromosomal rearrangements in the model legume L. japonicus, L. filicaulis, and L. burttii. This species is positioned in a sister clade of the previously-mapped species and is proposed as one of the progenitors of L. corniculatus, the main forage crop of the genus. The cytogenetic map allowed the location of 12 genomic regions to be compared between these species. A high macrosynteny was revealed, but it was interrupted by a translocation involving chromosomes 3 and 5, a new rearrangement for the genus. Also, a transposition on chromosome 2 was found in L. japonicus 'Miyakojima'. Furthermore, changes in the number, size, and position of rDNA sites were observed, as well as an intraspecific size heteromorphism of the 5S rDNA site on L. uliginosus chromosome 6. The karyotype differences observed are proportional to the phylogenetic distance among these species.

Microsatellite loci for Paspalum atratum (Poaceae) and cross-amplification in other species.

PREMISE OF THE STUDY: Paspalum atratum is a perennial, cespitose, tropical grass native to Central and South America. This species belongs to a polyploid complex (Plicatula group) little known at the genetic level. The characterized microsatellite markers provide new informative tools for further studies of the hybridization, mating systems, and structure of the population. • METHODS AND RESULTS: Using the microsatellite-enriched library method, we isolated and characterized 19 microsatellite markers from P. atratum. Eleven of them were polymorphic, showing a variable degree of variation, while eight were monomorphic in the samples analyzed. Additionally, the transferability of these microsatellite markers was tested in other species. • CONCLUSIONS: These results suggest that the characterized markers have enough discriminatory potential to be used in genetic characterizations of Paspalum taxa, which are based on an understanding of their mating systems and genetic structure, as well as in understanding the evolutionary processes involved in the evolution of groups of Paspalum.

Roles for Fis and YafK in biofilm formation by enteroaggregative Escherichia coli.

Enteroaggregative Escherichia coli (EAEC) forms thick biofilms on the intestinal mucosa. Here, we show that most EAEC strains form a biofilm on glass or plastic surfaces when grown in cell culture medium with high sugar and osmolarity. Biofilm-forming ability in two prototype EAEC strains required aggregative adherence fimbriae (AAF), although many other EAEC strains that do not express AAF also developed biofilms under these conditions. Ten thousand transposon mutants of EAEC strain 042 were isolated, and 100 were found to be deficient in biofilm formation. Of these, 93 were either deficient in in vitro growth or mapped to genes known to be required for AAF/II expression. Of the seven remaining insertions, five mapped to one of two unsuspected loci. Two insertions involved the E. coli chromosomal fis gene, a DNA-binding protein that is involved in growth phase-dependent regulation. Using reverse transcription-polymerase chain reaction (RT-PCR), we determined that the effect of fis was at the level of transcription of the AAF/II activator aggR. Biofilm formation also required the product of the yafK gene, which is predicted to encode a secreted 28 kDa protein. The yafK product is required for transcription of AAF/II-encoding genes. Our data do not suggest a role for type 1 fimbriae or motility in biofilm formation. EAEC appears to form a novel biofilm, which may be mediated solely by AAF and may reflect its interactions with the intestinal mucosa.

Child labor and health: problems and perspectives.

Child labor remains a widespread problem. Although it can have positive effects, in some situations it has negative effects on health and development of the children. Although mainly a problem in developing countries, it is also possible to find child workers, some working in hazardous activities, in developed countries. The authors describe the child labor profiles in developed and developing countries, the principal occupations of children, and their concomitant hazards. They summarize the epidemiologic evidence for a greater impact of some occupational exposures on the health of children as compared with adults, and the theoretical concerns about the impact of child labor on health, and suggest policies that can be used to combat harmful child labor.

[Association between individual and socioeconomic characteristics and work-related accidents in Pelotas, Southern Brazil]. / Associação entre as características individuais e sócio-econômicas e os acidentes do trabalho em Pelotas, Rio Grande do Sul, Brasil.

A case-control study was conducted to study the association between individual and socioeconomic characteristics and occupational accidents. Cases were 264 workers who had suffered a "typical" occupational accident registered at the National Institute of Social Security in the city of Pelotas in 1996. Workers were interviewed in their homes with a standardized questionnaire. Two fatal accidents were excluded, as were those leading to absence from work for less than seven days. For each case, three controls were selected: a colleague from the same workplace, a neighbor, and a population control. These were matched to cases by age (+/-5 years) and sex and had not suffered a work-related accident in the preceding month. All cases and controls had formal employment contracts and were residents in the urban area. Conditional logistic regression was used in the data analyses. Schooling and monthly income were the main determinants of occupational accidents. Effects of age, skin color, smoking, alcohol dependence (CAGE questionnaire), and stressful life events disappeared after adjustment for schooling and income.

[Workers' perception of exposure to workload and risks of accidents in a southern Brazilian city]. / Percepção de exposição a cargas de trabalho e riscos de acidentes em Pelotas, RS (Brasil).

OBJECTIVE: The study of the association between workers' perceptions of occupational hazards and the risk of occupational accidents. DESIGN: Case control study. POPULATION: The cases were 264 workers who presented a "typical" occupational accident, registered at the National Institute of Social Security in the city of Pelotas, between January and July, 1996. Fatal accidents (two) were excluded, as were those leading to an absence of less than seven days from work. The cases were interviewed in their homes with a standard questionnaire. For each case, three controls were chosen: a fellow-work, a neighbor and a population control. Controls were matched to the cases by age (+/- 5 years) and sex; workers who had suffered an occupational accident in the preceding month were excluded from the control group. All cases and controls were formally employed and lived in the urban area. The data were analyzed using conditional logistic regression. RESULTS AND CONCLUSIONS: The risk of occupational accidents was found to double among workers who reported having faced emergency situations at work, working in high places, facing constant danger or noisy environments. Working in uncomfortable positions or intense physical activities were associated with a 50% increase in risk. The remaining occupational hazards under study were not significantly associated with the risk of accidents. All of the above results were adjusted for confounding factors.

The Brazilian cohort of pulp and paper workers: the logistic of a cancer mortality study.

The International Agency for Research on Cancer (IARC) proposed this international historical cohort study trying to solve the controversy about the increased risk of cancer in the workers of the Pulp and Paper Industry. One of the most important aspects presented by this study in Brazil was the strategies used to overcome the methodological challenges, such as: data access, data accuracy, data availability, multiple data sources, and the large follow-up period. Through multiple strategies it was possible to build a Brazilian cohort of 3,622 workers, to follow them with a 93 percent success rate and to identify in 99 percent of the cases the cause of death. This paper, has evaluated the data access, data accuracy and the effectiveness of the strategies used and the different sources of data.