RESUMO
The diploid Lotus japonicus was previously suggested as a model for the legume plant family. We present here the nucleotide sequence and the derived gene organization of a small part of the genome in this model plant. Two functional genes with the same transcriptional orientation were identified within the 23326-bp genomic region analysed. The LjGln1 gene encodes a cytosolic glutamine synthetase and the LjKrm (Kinesin repeat motif) gene encodes a polypeptide with similarity to a repeated motif present in the microtubule-associated kinesin light chain protein. Transcripts of the glutamine synthetase gene are found primarily in roots and root nodules, while transcripts of the Krm gene are found in roots, root nodules and leaves. In the region between Gln1 and Krm, the presence of a third gene, Pge1, was suggested by analysis with the Grail exon recognition program. Upstream of the Gln1 gene a segment carrying two apparently non-functional, fragmented copia-like retroelements, dRtp1 and dRtp2, was identified. Sequence similarity to reverse transcriptase- and RNaseH-coding regions defined the defective retro-elements dRtp1 and dRtp2 within this segment. Terminal repeats were not found but three different sets of short direct repeats are located adjacent to the dRtp1 element. The three genes and the incomplete retroelements occupy most of the analysed DNA, leaving approximately one-fifth of the chromosome fragment without recognisable genome constituents.
Assuntos
Fabaceae/enzimologia , Fabaceae/genética , Genes de Plantas , Glutamato-Amônia Ligase/genética , Plantas Medicinais , Sequência de Aminoácidos , Sequência de Bases , Mapeamento Cromossômico , DNA de Plantas/genética , Expressão Gênica , Genoma de Planta , Cinesinas/genética , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Sequências Repetitivas de Ácido Nucleico , Retroelementos/genética , Homologia de Sequência de AminoácidosRESUMO
Soluble fibrin (SF) has attracted considerable interest as a marker for haemostatic activation. Two new enzyme immunoassays for SF, Enzymun-Test FM (Boehringer Mannheim, Germany) and Fibrinostika Soluble Fibrin (Organon Teknika, Belgium) have recently become commercially available. We measured plasma levels of SF in clinically obtained blood samples in order to compare the two new assays. Blood was drawn from 10 healthy volunteers and from 149 patients on the first day after surgery for fractures of the upper part of the femur. Collection and processing was done according to the manufacturers' recommendations. In the patients, levels found by the two assays were significantly different. The Enzymun-Test assay reported a median (range) of 11.87 (2.66 - > 62.20) mg/l, whereas the Fibrinostika assay found a median (range) of 3.34 (1.08 - >10) mg/l. The correlation coefficient was 0.38 (Spearman). A poor correlation was thus found between values obtained by the two assays in the patient category chosen. Further validation of the assays is necessary.
Assuntos
Ensaio de Imunoadsorção Enzimática , Fibrina/análise , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , SolubilidadeRESUMO
In order to compare levels of Prothrombin Fragment 1 + 2 (F1 + 2), Thrombin-antithrombin III complex (TAT), Fibrinogen Degradation Products (FgDP) and Fibrin Degradation Products (FbDP) in plasma from blood drawn into sodium citrate with and without the protease inhibitor D-Phe-Pro-Arg-Chloromethylketone (PPACK), blood samples were collected from 41 patients on the first day after elective gastric surgery. Levels of F1 + 2, TAT and FbDP were not significantly different in plasma with and without PPACK. FgDP levels were significantly higher in plasma with PPACK. Our results did not support previous suggestions that PPACK should be used as a routine anticoagulant for measurement of haemostatic activation products.