RESUMO
Hepatitis Delta is a disease caused by exposure to hepatitis B (HBV) and hepatitis D (HDV) viruses, usually with a more severe clinical outcome when compared to an HBV monoinfection. To date, the real prevalence of HDV infection is underestimated and detection methods are poorly available, especially in more endemic regions. Therefore, a one-step RT-qPCR method for quantification of HDV-RNA was developed. Biological samples were selected between 2017 and 2023 from patients at the Ambulatório Especializado em Hepatites Virais of the Centro de Pesquisa em Medicina Tropical de Rondônia and Serviço de Assistência Especializada and underwent the test developed by this study and a second quantitative RT-qPCR assay. The slope of the initial quantitative assay was - 3.321 with an efficiency of 100.04% and amplification factor equal to 2. Analysis of the repeatability data revealed a Limit of Quantification of 5 copies/reaction and Limit of Detection (95%) of 2.83 copies per reaction. In the diagnostic sensitivity tests, there was an accuracy of 97.37% when compared to the reference test. This assay proved to be highly efficient and reproducible, making it a valuable tool to monitor hepatitis Delta patients and assess the risk of disease progression, as well as the effectiveness of treatment.
Assuntos
Hepatite A , Hepatite B , Hepatite D , Humanos , Vírus Delta da Hepatite/genética , RNA Viral/genética , Hepatite D/epidemiologia , Vírus da Hepatite B/genéticaRESUMO
Tem como principal objetivo promover a avaliaçäo de uma metodologia educativa para identificar e refletir, criticamente sobre os riscos à saúde de adolescentes causados pelo trabalho em garimpos de ouro. O projeto foi realizado com adolescentes que estudavam na Escola Estadual Cônego Braga do Distrito de Monsehor Horta, Município de Mariana, Minas Gerais, Brasil. A avaliaçäo dos resultados através de testes estatísticos revelou um significativo acréscimo de respostas corretas da primeira para a última fase sobre a definiçäo de acidentes de trabalho e sua equiparaçäo com as doenças do trabalho, os acidentes de trajeto e as agressöoes no local de trabalho.