RESUMO
Calcium and bone metabolism remain key concerns for space travelers, and ground-based models of space flight have provided a vast literature to complement the smaller set of reports from flight studies. Increased bone resorption and largely unchanged bone formation result in the loss of calcium and bone mineral during space flight, which alters the endocrine regulation of calcium metabolism. Physical, pharmacologic, and nutritional means have been used to counteract these changes. In 2012, heavy resistance exercise plus good nutritional and vitamin D status were demonstrated to reduce loss of bone mineral density on long-duration International Space Station missions. Uncertainty continues to exist, however, as to whether the bone is as strong after flight as it was before flight and whether nutritional and exercise prescriptions can be optimized during space flight. Findings from these studies not only will help future space explorers but also will broaden our understanding of the regulation of bone and calcium homeostasis on Earth.
Assuntos
Desenvolvimento Ósseo , Reabsorção Óssea/etiologia , Medicina Baseada em Evidências , Modelos Biológicos , Estado Nutricional , Voo Espacial/história , Ausência de Peso/efeitos adversos , Animais , Conservadores da Densidade Óssea/uso terapêutico , Reabsorção Óssea/metabolismo , Reabsorção Óssea/prevenção & controle , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/metabolismo , Cálcio da Dieta/metabolismo , Cálcio da Dieta/uso terapêutico , História do Século XX , História do Século XXI , Humanos , Treinamento Resistido , Vitamina D/metabolismo , Vitamina D/uso terapêuticoRESUMO
High-protein and acidogenic diets induce hypercalciuria. Foods or supplements with excess sulfur-containing amino acids increase endogenous sulfuric acid production and therefore have the potential to increase calcium excretion and alter bone metabolism. In this study, effects of an amino acid/carbohydrate supplement on bone resorption were examined during bed rest. Thirteen subjects were divided at random into two groups: a control group (Con, n = 6) and an amino acid-supplemented group (AA, n = 7) who consumed an extra 49.5 g essential amino acids and 90 g carbohydrate per day for 28 days. Urine was collected for n-telopeptide (NTX), deoxypyridinoline (DPD), calcium, and pH determinations. Bone mineral content was determined and potential renal acid load was calculated. Bone-specific alkaline phosphatase was measured in serum samples collected on day 1 (immediately before bed rest) and on day 28. Potential renal acid load was higher in the AA group than in the Con group during bed rest (P < 0.05). For all subjects, during bed rest urinary NTX and DPD concentrations were greater than pre-bed rest levels (P < 0.05). Urinary NTX and DPD tended to be higher in the AA group (P = 0.073 and P = 0.056, respectively). During bed rest, urinary calcium was greater than baseline levels (P < 0.05) in the AA group but not the Con group. Total bone mineral content was lower after bed rest than before bed rest in the AA group but not the Con group (P < 0.05). During bed rest, urinary pH decreased (P < 0.05), and it was lower in the AA group than the Con group. These data suggest that bone resorption increased, without changes in bone formation, in the AA group.
Assuntos
Aminoácidos Essenciais/administração & dosagem , Aminoácidos Essenciais/efeitos adversos , Reabsorção Óssea/induzido quimicamente , Reabsorção Óssea/metabolismo , Calcificação Fisiológica/efeitos dos fármacos , Suplementos Nutricionais/efeitos adversos , Simulação de Ausência de Peso/efeitos adversos , Adulto , Fosfatase Alcalina/sangue , Aminoácidos/urina , Cálcio/metabolismo , Colágeno/urina , Colágeno Tipo I , Humanos , Masculino , Peptídeos/urinaRESUMO
Because of the variability of collagen crosslinks, their use as markers for bone resorption is often criticized. We hypothesized that the variability could be reduced by collecting urine for 24 hours (or longer) instead of using single voids, and by not normalizing to creatinine. Urine samples were collected from 22 healthy subjects during two or more 24-hour periods. Each 24-hour pool and each 2nd void of the day were analyzed for N-telopeptide (NTX), pyridinium (PYD), and deoxypyridinoline (DPD) crosslinks. Data were analyzed by using linear regression. For NTX, R2 for the two, 2nd-void samples (n = 38) was 0.55, whereas R2 for the two 24-hour pools was 0.51 or 0.52, expressed per day or per creatinine. For PYD and DPD, R2 for the 2nd-void samples was 0.26 and 0.18, R2 for the 24-hour pools expressed per day was 0.58 and 0.74, and R2 for the 24-hour pools expressed per creatinine was 0.65 and 0.76, respectively. Regression of the 2nd void and the corresponding 24-hour pool, expressed per day, yielded R2 = 0.19, 0.19, and 0.08, for NTX, PYD, and DPD, respectively (n = 76 each). For the 2nd-void sample and its corresponding 24-hour pool, expressed per creatinine, R2 = 0.24, 0.33, and 0.08, respectively. In a separate study, the coefficient of variation for NTX was reduced (P < 0.05) when data from more than one 24-hour collection were combined. Thus, the variability inherent in crosslink determinations can be reduced by collecting urine for longer periods. In research studies, the high variability of single-void collections, compounded by creatinine normalization, may alter or obscure findings.
Assuntos
Aminoácidos/urina , Colágeno/urina , Peptídeos/urina , Compostos de Piridínio/urina , Manejo de Espécimes/métodos , Adulto , Biomarcadores/análise , Colágeno Tipo I , Feminino , Humanos , Masculino , Reprodutibilidade dos TestesRESUMO
Adequate nutrition is critical during long-term spaceflight, as is the ability to easily monitor dietary intake. A comprehensive nutritional status assessment profile was designed for use before, during and after flight. It included assessment of both dietary intake and biochemical markers of nutritional status. A spaceflight food-frequency questionnaire (FFQ) was developed to evaluate intake of key nutrients during spaceflight. The nutritional status assessment protocol was evaluated during two ground-based closed-chamber studies (60 and 91 d; n = 4/study), and was implemented for two astronauts during 4-mo stays on the Mir space station. Ground-based studies indicated that the FFQ, administered daily or weekly, adequately estimated intake of key nutrients. Chamber subjects maintained prechamber energy intake and body weight. Astronauts tended to eat 40--50% of WHO-predicted energy requirements, and lost >10% of preflight body mass. Serum ferritin levels were lower after the chamber stays, despite adequate iron intake. Red blood cell folate concentrations were increased after the chamber studies. Vitamin D stores were decreased by > 40% on chamber egress and after spaceflight. Mir crew members had decreased levels of most nutritional indices, but these are difficult to interpret given the insufficient energy intake and loss of body mass. Spaceflight food systems can provide adequate intake of macronutrients, although, as expected, micronutrient intake is a concern for any closed or semiclosed food system. These data demonstrate the utility and importance of nutritional status assessment during spaceflight and of the FFQ during extended-duration spaceflight.
Assuntos
Dieta , Ingestão de Energia , Micronutrientes/administração & dosagem , Avaliação Nutricional , Ausência de Peso/efeitos adversos , Adulto , Biomarcadores , Feminino , Ferritinas/sangue , Ácido Fólico/sangue , Humanos , Masculino , Micronutrientes/sangue , Estado Nutricional , Voo Espacial , Simulação de Ambiente Espacial , Inquéritos e Questionários , Vitamina D/sangue , Redução de PesoRESUMO
The loss of bone during spaceflight is considered a physiological obstacle for the exploration of other planets. This report of calcium metabolism before, during, and after long-duration spaceflight extends results from Skylab missions in the 1970s. Biochemical and endocrine indexes of calcium and bone metabolism were measured together with calcium absorption, excretion, and bone turnover using stable isotopes. Studies were conducted before, during, and after flight in three male subjects. Subjects varied in physical activity, yet all lost weight during flight. During flight, calcium intake and absorption decreased up to 50%, urinary calcium excretion increased up to 50%, and bone resorption (determined by kinetics or bone markers) increased by over 50%. Osteocalcin and bone-specific alkaline phosphatase, markers of bone formation, increased after flight. Subjects lost approximately 250 mg bone calcium per day during flight and regained bone calcium at a slower rate of approximately 100 mg/day for up to 3 mo after landing. Further studies are required to determine the time course of changes in calcium homeostasis during flight to develop and assess countermeasures against flight-induced bone loss.
Assuntos
Cálcio/metabolismo , Voo Espacial , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de TempoRESUMO
This study was designed to validate the utility of a commercial portable clinical blood analyzer (PCBA) in ground-based studies and on the space shuttle. Ionized calcium, pH, electrolytes, glucose, and hematocrit were determined. Results agreed well with those from traditional laboratory methods, and the PCBA demonstrated good between-day precision for all analytes. In-flight analysis of control samples revealed differences in one analyte (sodium). There were few changes in crew members' results during flight, and these were expected. Potassium increased in flight compared with before flight, and potassium, pH, and hematocrit decreased after flight. Ionized calcium was decreased in flight and on landing day. Changes during flight were likely related to sample collection technique. Postflight changes likely reflected the fluid redistribution that occurs after exposure to weightlessness. These data confirm that the PCBA is a reliable instrument for most analytes, and can provide important medical data in remote locations, such as orbiting spacecraft.
Assuntos
Medicina Aeroespacial/métodos , Análise Química do Sangue/instrumentação , Voo Espacial , Adulto , Análise Química do Sangue/métodos , Glicemia/análise , Coleta de Amostras Sanguíneas , Cálcio/sangue , Estudos de Avaliação como Assunto , Feminino , Hematócrito , Humanos , Concentração de Íons de Hidrogênio , Masculino , Potássio/sangue , Reprodutibilidade dos Testes , Sódio/sangueRESUMO
Norepinephrine (NE) turnover rate was determined in several tissues of 5-week-old female mice fed a high carbohydrate diet (58% of energy as carbohydrate, 30% fat) either ad lib or restricted to 34 or 24 kJ/day (36 to 50% restriction) presented as 1 or 2 daily meals. When the restricted intakes were divided into 2 equal meals, daily NE turnover did not differ from that of ad lib-fed mice. When the above restricted amounts were provided as a single daily meal at the beginning of the dark period, NE turnover was 38% and 46% lower, respectively, in the heart only compared to ad lib-fed controls. Serum glucose and total free fatty acids were affected by dietary conditions known to produce sympathetic activation (high carbohydrate and high fat diets) and suppression (high protein diet and energy restriction as a single meal), but the changes were unrelated to fractional NE turnover. Thus, the lower NE turnover seen when food intake is restricted is due to the prolonged overnight fast and not due to the lower energy intake per se, and is not associated with serum concentration of glucose or total free fatty acids.